Initial Bacterial Adhesion Research Articles

Quantitative study of early-stage transient bacterial adhesion to bioactive glass and glass ceramics: atomic force microscopic observations

Antimicrobial potential of bioactive glass (BAG) makes it promising for implant applications, specifically overcoming the toxicity concerns associated with traditional antibacterial nanoparticles. The 58S composition of BAG (with high Ca and absence of Na) has been known to exhibit excellent bioactivity and antibacterial behaviour, but the mechanisms behind have not been investigated in detail. In this pioneering study, we are using Atomic Force Microscopy (AFM) to gain insights into 58S BAG’s adhesive interactions with planktonic cells of both gram-positive (Staphylococcus aureus) and gram-negative (Escherichia coli) bacteria; along with the impact of crystallinity on antibacterial properties. We have recorded greater bacterial inhibition by amorphous BAG compared to semi-crystalline glass–ceramics and stronger effect against gram-negative bacteria via conventional long-term antibacterial tests. AFM force distance curves has illustrated substantial bonding between bacteria and BAG within the initial one second (observed at a gap of 250 ms) of contact, with multiple binding events. Further, stronger adhesion of BAG with E.coli (~ 6 nN) compared to S. aureus (~ 3 nN) has been found which can be attributed to more adhesive nano-domains (size effect) distributed uniformly on E.coli surface. This study has revealed direct evidence of impact of contact time and 58S BAG’s crystalline phase on bacterial adhesion and antimicrobial behaviour. Current study has successfully demonstrated the mode and mechanisms of initial bacterial adhesion with 58S BAG. The outcome can pave the way towards improving the designing of implant surfaces for a range of biomedical applications.

Does dental material type influence bacterial adhesion under the same polishing conditions? Direct observation using a fluorescent staining technique: An in vitro study.

Highly polished 3, 4, and 5 mol% yttria-stabilized zirconia and CAD/CAM composite resin samples were prepared, and the influence of surface roughness (Ra and Sa, 21 areas/group), wettability (contact angle and surface energy, 3 samples/group), and surface chemical composition (2 samples/group) on single-strain bacterial adhesion models (Porphyromonas gingivalis, Streptococcus oralis, Streptococcus sanguinis, Streptococcus gordonii, and Streptococcus mutans) were compared via fluorescent staining with graphical analysis (21 areas/group). Statistical analysis was performed using the Shapiro-Wilk test followed by one-way analysis of variance with Tukey's test or the Kruskal-Wallis test with Dunn's test (α=0.05) and linear regression. For dental zirconia with the same surface roughness, the yttria content did not significantly influence the initial bacterial adhesion. However, higher bacterial adhesion was detected for the composite resin owing to its high C, O, and Si contents. There was no correlation between surface energy and bacterial adhesion for any bacterial strain (p<0.005).

A comparative study on the mechanical and antibacterial properties of BPA-free dental resin composites

ObjectiveThe commonly used base monomer utilized in resinous commercial dental restorative products is bis-GMA which is derived from bisphenol-A (BPA) - a well-known compound which may disrupt endocrine functions. To address concerns about its leaching into the oral environment and to optimize the quality of dental composites, a BPA-free alternative base monomer, fluorinated urethane dimethacrylate (FUDMA), was designed by modifying a UDMA monomer system. MethodsNine groups of composites were prepared by mixing the base monomers and TEGDMA in a ratio of 70/30 wt% to which were added silanized glass particles (mean diameter: 0.7 µm) in 3 different volume fractions (40, 45, and 50 vol%). Bis-GMA and UDMA base monomers were used as control groups in the same ratios. Various properties including degree of conversion (DC), flexural strength (FS) and flexural modulus (FM), water sorption (WS), solubility (SL), surface hardness and roughness, and initial adhesion property against S.mutans were investigated. One-way analysis of variance followed by Bonferroni test at α = 0.05 was used to analyze the results. ResultsA significant difference in FS between FUDMA-based composite with 40 vol% filler (120.3 ± 10.4 MPa) and Bis-GMA-based composite with the same filler fraction (105.8 ± 10.0 MPa) was observed but there was no significant difference among other groups. The UDMA based group exhibited the highest WS (1.3 ± 0.3 %). Bis-GMA showed greater initial bacterial adhesion but was not statistically different from the other groups (p = 0.082). SignificanceFUDMA-based resin composites exhibit comparable mechanical and bacterial adhesion properties compared with Bis-GMA and UDMA-based composites. The FUDMA composites show positive outcomes indicating they could be used as substitute composites to Bis-GMA-based composites.

Diclofenac sodium effectively inhibits the biofilm formation of Staphylococcus epidermidis.

Staphylococcus epidermidis is an opportunistic pathogen commonly implicated in medical device-related infections. Its propensity to form biofilms not only leads to chronic infections but also exacerbates the issue of antibiotic resistance, necessitating high-dose antimicrobial treatments. In this study, we explored the use of diclofenac sodium, a non-steroidal anti-inflammatory drug, as an anti-biofilm agent against S. epidermidis. In this study, crystal violet staining and confocal laser scanning microscope analysis showed that diclofenac sodium, at subinhibitory concentration (0.4 mM), significantly inhibited biofilm formation in both methicillin-susceptible and methicillin-resistant S. epidermidis isolates. MTT assays demonstrated that 0.4 mM diclofenac sodium reduced the metabolic activity of biofilms by 25.21-49.01% compared to untreated controls. Additionally, the treatment of diclofenac sodium resulted in a significant decrease (56.01-65.67%) in initial bacterial adhesion, a crucial early phase of biofilm development. Notably, diclofenac sodium decreased the production of polysaccharide intercellular adhesin (PIA), a key component of the S. epidermidis biofilm matrix, in a dose-dependent manner. Real-time quantitative PCR analysis revealed that diclofenac sodium treatment downregulated biofilm-associated genes icaA, fnbA, and sigB and upregulated negative regulatory genes icaR and luxS, providing potential mechanistic insights. These findings indicate that diclofenac sodium inhibits S. epidermidis biofilm formation by affecting initial bacterial adhesion and the PIA synthesis. This underscores the potential of diclofenac sodium as a supplementary antimicrobial agent in combating staphylococcal biofilm-associated infections.

Responsive antibacterial surface based on looped poly(methacrylic acid)

The adhesion of bacteria to surfaces poses a significant challenge to public health and many industries, making the development of antibacterial surfaces an urgent issue. In this work, α,ω-bisthiol poly(methacrylic acid) (HS-PMAA-SH) was synthesized and grafted in loop conformation onto a substrate primed with polydopamine, in conjunction with Ag NPs prepared in-situ to create a pH responsive composite surface with synergistic fouling-resistant and bacterial killing and releasing capabilities. A monofunctional analogue of half the chain length, PMAA-SH, was also synthesized and used to construct the composite surface with the PMAA in linear conformation for comparison. Quartz crystal microbalance with dissipation experiments revealed that PMAA loops allowed much less protein to adsorb than their linear analogues. Confocal laser scanning microscopy results showed that both composite surfaces were effective in killing the bacteria attached due to the presence of Ag NPs, and exhibited good initial resistance to bacterial adhesion and ability to release the killed bacteria under mild conditions due to the PMAA component; the composite surface conjugated with PMAA-loop was superior to the one with PMAA-linear, presenting lower initial bacterial adhesion and higher release rates, maintaining a low bacterial coverage even after five resist/kill/release cycles. The research opens a new avenue for antibacterial surfaces that integrate fouling-resistance, bacterial killing and release functions.

Biofilm Formation on Hybrid, Resin-Based CAD/CAM Materials for Indirect Restorations: A Comprehensive Review.

Hybrid materials are a recent addition in the field of restorative dentistry for computer-aided design/computer-aided manufacturing (CAD/CAM) indirect restorations. The long-term clinical success of modern dental restorative materials is influenced by multiple factors. Among the characteristics affecting the longevity of a restoration, the mechanical properties and physicοchemical interactions are of utmost importance. While numerous researchers constantly evaluate mechanical properties, the biological background of resin-based CAD/CAM biomaterials is scarcely investigated and, therefore, less described in the literature. This review aims to analyze biofilm formation on the surfaces of novel, hybrid, resin-based CAD/CAM materials and evaluate the methodological protocols followed to assess microbial growth. It is demonstrated that the surface structure, the composition and the finishing and polishing procedures on the surface of a dental restorative material influence initial bacterial adhesion; however, most studies focus on in vitro protocols, and in vivo and/or in situ research of microbiomics in CAD/CAM restorative materials is lacking, obstructing an accurate understanding of the bioadhesion phenomenon in the oral cavity.

Microbiologically influenced corrosion can cause a dental implant rejection

In the present study, we apply electrochemical and molecular biology approaches to establish whether microbiologically influenced corrosion (MIC) may be the reason for dental implant rejection. Titanium-based implants have been incubated in vitro in Ringer/glucose solution in the presence and absence of a salivary microbiota and the change in their corrosion potential and current density was monitored over time by potentiodynamic polarization. The charge transfers processes occurring on the Ti-based implant surface during the initial bacterial adhesion and after biofilm formation were analyzed by electrochemical impedance spectroscopy. The components of the equivalent electrical circuit as well as the heterogeneous electron transfer rate constants were estimated. In parallel, bacterial species from the salivary microbiota have been identified by sequencing of the 16S ribosomal amplicon method, and their contribution to the corrosion processes of dental implants was verified and compared to that of native saliva in vitro. The scanning electron microscopy observation and energy dispersive spectroscopy analysis of the implant surface after long-term exposure to a bacterial-containing environment revealed more detailed features of the occurring corrosion processes.

Visible-light photoactivated proanthocyanidin and kappa-carrageenan coating with anti-adhesive properties against clinically relevant bacteria

The increase of bacterial resistance to antibiotics is a growing concern worldwide and the search for new therapies could cost billions of dollars and countless lives. Inert surfaces are major sources of contamination due to easier adhesion and formation of bacterial biofilms, hindering the disinfection process. Therefore, the objective of this study was to develop a photoactivatable and anti-adhesive kappa-carrageenan coating using proanthocyanidin as a photosensitizer. The complete reduction (>5-log10 CFU/cm3) of culturable cells of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa pathogens was achieved after 30 min of exposure to visible light (420 nm; 30 mW/cm2) with 5 % (w/v) of the photosensitizer. Cell membrane damage was confirmed by measuring potassium leakage, epifluorescence microscopy and bacterial motility analysis. Overall, visible light irradiation on coated solid surfaces mediated by proanthocyanidin showed no cytotoxicity and inactivated clinically important pathogens through the generation of reactive oxygen species, inhibiting bacterial initial adhesion. The developed coating is a promising alternative for a wide range of applications related to surface disinfection and food biopreservation.

Factors influencing initial bacterial adhesion to antifouling surfaces studied by single-cell force spectroscopy

Biofilm formation, a major concern for healthcare systems, is initiated when bacteria adhere to surfaces. Escherichia coli adhesion is mediated by appendages, including type-1 fimbriae and curli amyloid fibers. Antifouling surfaces prevent the adhesion of bacteria to combat biofilm formation. Here, we used single-cell force-spectroscopy to study the interaction between E.coli and glass or two antifouling surfaces: the tripeptide DOPA-Phe(4F)-Phe(4F)-OMe and poly(ethylene glycol) polymer-brush. Our results indicate that both antifoulants significantly deter E.coli initial adhesion. By using two mutant strains expressing no type-1 fimbriae or curli amyloids, we studied the adhesion mechanism. Our results suggest that the bacteria adhere to different antifoulants via separate mechanisms. Finally, we show that some bacteria adhere much better than others, illustrating how the variability of bacterial cultures affects biofilm formation. Our results emphasize how additional study at the single-cell level can enhance our understanding of bacterial adhesion, thus leading to novel antifouling technologies.

Nanotopography and oral bacterial adhesion on titanium surfaces: in vitro and in vivo studies.

The present study aimed to evaluate the influence of titanium surface nanotopography on the initial bacterial adhesion process by in vivo and in vitro study models. Titanium disks were produced and characterized according to their surface topography: machined (Ti-M), microtopography (Ti-Micro), and nanotopography (Ti-Nano). For the in vivo study, 18 subjects wore oral acrylic splints containing 2 disks from each group for 24 h (n = 36). After this period, the disks were removed from the splints and evaluated by microbial culture method, scanning electron microscopy (SEM), and qPCR for quantification of Streptococcus oralis, Actinomyces naeslundii, Fusobacterium nucleatum, as well as total bacteria. For the in vitro study, adhesion tests were performed with the species S. oralis and A. naeslundii for 24 h. Data were compared by ANOVA, with Tukey's post-test. Regarding the in vivo study, both the total aerobic and total anaerobic bacteria counts were similar among groups (p > 0.05). In qPCR, there was no difference among groups of bacteria adhered to the disks (p > 0.05), except for A. naeslundii, which was found in lower proportions in the Ti-Nano group (p < 0.05). In the SEM analysis, the groups had a similar bacterial distribution, with a predominance of cocci and few bacilli. In the in vitro study, there was no difference in the adhesion profile for S. oralis and A. naeslundii after 24 h of biofilm formation (p > 0.05). Thus, we conclude that micro- and nanotopography do not affect bacterial adhesion, considering an initial period of biofilm formation.

The influence of surface topography and microstructure of zirconia on initial bacterial adhesion of streptococcus mutans– in vitro study

The purpose of this research in this paper is to study the physical and chemical characteristics of the surfaces and the topography of the surfaces of zirconium materials used for the processing of fixed prosthetic works and their influence on the surface adhesion of Streptococcus mutans bacteria. The samples used for examination are produced from zircon material, they are divided into two groups, the first group after sintering are polished without glaze, while the second group, after sintering, are covered with a layer of glaze. For the realization of the experimental part of this research, the following methods and analyzes were used: surface roughness measurement (SR), contact angle measurement, surface free energy determination (SFE), biofilm formation and preparation for scanning with a microscope electronic (SEM) Specimens produced from zirconia, polished but unglazed showed higher contact angle values 90.340, while glazed samples showed lower values than unglazed ones 61.640, Surface free energy measurements of zirconia samples polished without glaze showed significantly lower values (24.31mJ/m2 ) compared to the values obtained in the zircon samples covered with glaze (40.66mJ/m2 ), The surface roughness values are higher in unglazed zirconia samples and is 0.65μm, while in the polished and glazed zirconia samples, the roughness of the surfaces is less 0.29μm, SEM analysis results show that the smallest number of attached bacteria is found on the surfaces of samples with unglazed zircon (8 bacteria), while in samples with zircon covered with glaze, a much larger number of bacteria (26 bacteria) were found on their surfaces. The roughness of the surfaces was shown to have no effect on the bacterial surface adhesion of these samples.

Food grade disinfectants based on hydrogen peroxide/peracetic acid and sodium hypochlorite interfere with the adhesion of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Listeria monocytogenes to stainless steel of differing surface roughness

This study aimed to evaluate the potential of the bacterium Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Listeria monocytogenes to adhere to stainless steel discs with differing degrees of surface roughness (Ra = 25.20–961.90 nm). Stainless steel is a material commonly used in the food industry for processing equipment, which is regularly exposed to cleaning procedures. The investigation included the commercial disinfectants hydrogen peroxide/peracetic acid and sodium hypochlorite which were evaluated for their antibacterial and anti-adhesion activity. The adhesion was assessed by the standard plate count method, while the broth microdilution method CLSI M07-A10 was used to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the disinfectants. Based on the MIC values, both disinfectants exerted significant inhibitory effects with MIC values for hydrogen peroxide/peracetic acid and sodium hypochlorite of 250 µg ml−1 and 500 µg ml−1, respectively. Whereas the MBC values were equal to the MIC for all bacteria except for E. coli with values 2-fold higher than the MIC. Obtained results also revealed that all tested bacteria were able to adhere to stainless steel surfaces, although differences were found for strains and surface roughness. The lowest adhesion rate of each strain was recorded on the roughest stainless steel disc at a Ra of 961.90 nm. Further, at a concentration of 1 MIC, the disinfectant sodium hypochlorite reduced initial bacterial adhesion to stainless steel surfaces to a significantly greater extent than the disinfectant hydrogen peroxide/peracetic acid. These findings are consistent with the results obtained by Scanning Electron Microscopy (SEM) analysis, which indicates the great applicability of the tested disinfectants for the control of bacterial adhesion in the food industry.

Supramolecular Self-Healing Antifouling Coating for Dental Materials.

In orthodontic treatment, orthodontic appliances are prone to bacterial infections, which pose a risk to oral health. Surface modification of orthodontic appliances has been explored to improve their antifouling properties and impart antibacterial capabilities, inhibiting initial bacterial adhesion and biofilm formation. However, coatings are susceptible to damage in the complex oral environment, leading to a loss of functionality. Here, we have prepared an antifouling self-healing coating based on supramolecular bonding by employing a simple spin coating method. The presence of the hydrophilic zwitterionic trimethylamine N-oxide (TMAO) and the hydrophobic antimicrobial moieties triclosan acrylate (TCSA) imparts to the polymers an amphiphilic structure and enhances the interaction with bacteria, resulting in excellent antimicrobial activity and surface antifouling properties. The multiple hydrogen bonds of ureido-pyrimidinone methacrylate (UPyMA) and ionic interactions contained in the polymers not only increased the adhesion of the coating to the material substrate (approximately 3 times) but also endowed the coating with the intrinsic self-healing ability to restore the antibiofouling properties at oral temperature and humidity. Finally, the polymer coating is biologically safe both in vitro and in vivo, showing no cytotoxic effects on cells and tissues. This research offers a promising avenue for improving the performance of orthodontic appliances and contributes to the maintenance and treatment of oral health.

Enhanced bacterial adhesion force by rifampicin resistance promotes microbial colonization on PE plastic compared to non-resistant biofilm formation

The microbial biofilm formed on plastics, is ubiquitous in the environment. However, the effects of antibiotic resistance on the development of the biofilm on plastics, especially with regard to initial cell attachment, remain unclear. In this study, we investigated the initial bacterial adhesion and subsequent biofilm growth of a rifampin (Rif) resistant E. coli (RRE) and a normal gram-positive B. subtilis on a typical plastic (polyethylene, PE). The experiments were conducted in different antibiotic solutions, including Rif, sulfamethoxazole (SMX), and kanamycin (KM), with concentrations ranging from 1 to 1000 μg/L to simulate different aquatic environments. The AFM-based single-cell adhesion force determination revealed that Rif resistance strengthened the adhesion force of RRE to PE in the environment rich in Rif rather than SMX and KM. The enhanced adhesion force may be due to the higher secretion of extracellular polymeric substances (EPS), particularly proteins, by RRE in the presence of Rif compared to the other two antibiotics. In addition, the higher ATP level of RRE would facilitate the initial adhesion and subsequent biofilm growth. Transcriptome analysis of RRE separately cultured in Rif and SMX environments demonstrated a clear correlation between the expression of Rif resistance and the augmented bacterial adhesion and cellular activity. Biofilm biomass analysis confirmed the promotion effect of Rif resistance on biofilm growth when compared to non-resistant biofilms, establishing a novel association with the augmentation of microbial adhesion force. Our study highlights concerns related to the dissemination of antibiotic resistance during microbial colonization on plastic that may arise from antibiotic resistance.

Tetraspanin CD9-derived peptides inhibit Pseudomonas aeruginosa corneal infection and aid in wound healing of corneal epithelial cells

Pseudomonas aeruginosa is a leading cause of corneal infection both within India and globally, often causing a loss of vision. Increasing antimicrobial resistance among the bacteria is making its treatment more difficult. Preventing initial bacterial adherence to the host membrane has been explored here to reduce infection of the cornea. Synthetic peptides derived from human tetraspanin CD9 have been shown to reduce infection in corneal cells both in vitro, ex vivo and in vivo. We found constitutive expression of CD9 in immortalized human corneal epithelial cells by flow cytometry and immunocytochemistry. The synthetic peptides derived from CD9 significantly reduced bacterial adherence to cultured corneal epithelial cells and ex vivo human cadaveric corneas as determined by colony forming units. The peptides also significantly reduced bacterial burden in a murine model of Pseudomonas keratitis and lowered the cellular infiltration in the corneal stroma. Additionally, the peptides aided corneal wound healing in uninfected C57BL/6 mice compared to control mice. These potential therapeutics had no effect on cell viability or proliferation of corneal epithelial cells and have the potential to be developed as an alternative therapeutic intervention.

Minimizing bacterial adhesion on membrane: Multiscale characterization of surface modifications

The control of biofouling is required to improve membrane performance for water filtration. In this context, developing bioinspired approaches to modify membranes and give them antifouling properties is an interesting alternative. In this study, we elaborated new anti-biofouling membranes by coating them with vanillin, a natural bioactive molecule acting as a Quorum Sensing Inhibitor (QSI). The adsorption of vanillin by filtration was optimized to obtain homogeneous surface modifications. The modified membranes were then characterized in terms of physical-chemical properties: the results showed that they were more hydrophobic and exhibited a 14% decrease in pure water flux. Then, their anti-fouling properties were evaluated using complementary multiscale characterization methodologies: AFM single-cell experiments showed that vanillin adsorption had an effect on both the probability of bacterial cells to adhere to the surface, and on the force of adhesion. Direct EFM and SEM observation and adhesion assays further confirmed the effect of vanillin-modified membranes at the population-scale which showed a decrease in the bacterial coverage rate up to 50%. Altogether, it confirms the multifunctionality of vanillin-coated membranes against biofouling: reduction of bacterial initial adhesion by changing the membranes surface properties and reduction of biofilm formation by quorum sensing inhibition.

Isoreticular chemistry guided enzymodynamic domino effect for biofilm microenvironment-responsive disinfection

Nanozyme therapy holds promise for overcoming daunting antimicrobial resistance, but skills to target the microbial microenvironment are lacking. Herein, an isoreticular principle was introduced in nanozyme discovery to directedly evolve a domino biomimetic reaction specific to drug-resistant bacterial biofilm. The isoreticular Ce-UiO-66-X (X = BDC, BDC-CH3, BDC-OH, BDC-NH2, BDC-NO2, ADC, Fum) MOFs exhibited linker-dependent mimicry of hydrolytic apyrase and redox oxidase, and the phosphorous reactants (ATP/ADP/AMP/Pi) produced by the ATP hydrolysis could spontaneously activate redox reaction in Ce-UiO-66-X. Evidenced by the representative Ce-BDC-X (X = –NO2, -H, and –NH2) nanozymes, the unique domino reaction of Ce-UiO-66-X could simultaneously deplete ATP molecules and trigger enhanced generation of superoxide radicals at the specific time window in the initial bacterial adhesion stage of methicillin-resistant Staphylococcus aureus (MRSA) biofilm formation. Due to the synergistic nanocatalytic mechanism, possible nanoscale penetration effect, and good biocompatibility, Ce-UiO-66-NO2 could effectively target and destroy the metabolic microenvironment of MRSA biofilm in vitro and in vivo, reducing the MRSA biofilm viability to as low as 3.64%. This work demonstrates a new prodrug-like enzymodynamic antibacterial therapeutics based on cooperative multienzyme reactivity.

Smart Implant with Bacteria Monitoring and Killing Ability for Orthopedic Applications.

Bacterial infections around implants constitute a significant cause of implant failures. Early recognition of bacterial adhesion is an essential factor in preventing implant infections. Therefore, an implant capable of detecting and disinfecting initial bacterial adhesion is required. This study reports on the development of an intelligent solution for this issue. We developed an implant integrated with a biosensor electrode based on alternating current (AC) impedance technology to monitor the early growth process of Escherichia coli (E. coli) and its elimination. The biosensor electrode was fabricated by coating polypyrrole (PPy) doped with sodium p-toluenesulfonate (TSONa) on titanium (Ti) surfaces. Monitoring the change in resistance using electrochemical impedance spectroscopy (EIS), combined with an equivalent circuit model (ECM), enables the monitoring of the early adhesion of E. coli. The correlation with the classical optical density (OD) monitoring value reached 0.989. Subsequently, the eradication of bacteria on the electrode surface was achieved by applying different voltages to E. coli cultured on the electrode surface, which caused damage to E. coli. Furthermore, in vitro cellular experiments showed that the PPy coating has good biocompatibility and can promote bone differentiation.

Recent Advances in Dual-Function Superhydrophobic Antibacterial Surfaces.

Bacterial adhesion and subsequent biofilm formation on the surfaces of synthetic materials imposes a significant burden in various fields, which can lead to infections in patients or reduce the service life of industrial devices. Therefore, there is increasing interest in imbuing surfaces with antibacterial properties. Bioinspired superhydrophobic surfaces with high water contact angles (>150°) exhibit excellent surface repellency against contaminations, thereby preventing initial bacterial adhesion and inhibiting biofilm formation. However, conventional superhydrophobic surfaces typically lack long-term durability and are incapable of achieving persistent efficacy against bacterial adhesion. To overcome these limitations, in recent decades, dual-function superhydrophobic antibacterial surfaces with both bacteria-repelling and bacteria-killing properties have been developed by introducing bactericidal components. These surfaces have demonstrated improved long-term antibacterial performance in addressing the issues associated with surface-attached bacteria. This review summarizes the recent advancements of these dual-function superhydrophobic antibacterial surfaces. First, a brief overview of the fabrication strategies and bacteria-repelling mechanism of superhydrophobic surfaces is provided and then the dual-function superhydrophobic antibacterial surfaces are classified into three types based on the bacteria-killing mechanism: i) mechanotherapy, ii) chemotherapy, and iii) phototherapy. Finally, the limitations and challenges of current research are discussed and future perspectives in this promising area are proposed.

Biphasic Textures Reducing Bacterial Surface Colonization in the Human Oral Cavity

Bacterial colonization occurs on all biological and artificial surfaces in the oral cavity. The formation of multicellular biofilms or settlement of misallocated species can cause caries (diet‐depending acidification), periodontal diseases, peri‐implantitis, or denture‐associated stomatitis. Inhibition or delay of initial adhesion should strongly reduce dental interventions. However, no strategy is found to mildly manage bacterial colonization in this complex physiological environment, that is, without toxic, antibacterial, or antiseptic approaches. It is shown in a previous study that micro‐ and submicrometer surface topographies, which can effectively control initial bacterial adhesion in other application areas, do not have any significant impact within the oral cavity. Herein, a simple approach is presented to reduce initial microbial surface colonization by plain biphasic textures with defined combinations of hydrophobic and hydrophilic phases (SiO2, NH2, CH2, CH3, F3). A significant reduction of microbial adhesion on textures in the nano‐ and microscale (150 nm and 2.4 μm) compared to respective monophasic substrates is observed. This might be a new design principle for dental materials, to inhibit microbial colonization in critical scenarios, at least for shorter time scales (&lt;24 h).