The influence of surface topography and microstructure of zirconia on initial bacterial adhesion of streptococcus mutans– in vitro study

Abstract

The purpose of this research in this paper is to study the physical and chemical characteristics of the surfaces and the topography of the surfaces of zirconium materials used for the processing of fixed prosthetic works and their influence on the surface adhesion of Streptococcus mutans bacteria. The samples used for examination are produced from zircon material, they are divided into two groups, the first group after sintering are polished without glaze, while the second group, after sintering, are covered with a layer of glaze. For the realization of the experimental part of this research, the following methods and analyzes were used: surface roughness measurement (SR), contact angle measurement, surface free energy determination (SFE), biofilm formation and preparation for scanning with a microscope electronic (SEM) Specimens produced from zirconia, polished but unglazed showed higher contact angle values 90.340, while glazed samples showed lower values than unglazed ones 61.640, Surface free energy measurements of zirconia samples polished without glaze showed significantly lower values (24.31mJ/m2 ) compared to the values obtained in the zircon samples covered with glaze (40.66mJ/m2 ), The surface roughness values are higher in unglazed zirconia samples and is 0.65μm, while in the polished and glazed zirconia samples, the roughness of the surfaces is less 0.29μm, SEM analysis results show that the smallest number of attached bacteria is found on the surfaces of samples with unglazed zircon (8 bacteria), while in samples with zircon covered with glaze, a much larger number of bacteria (26 bacteria) were found on their surfaces. The roughness of the surfaces was shown to have no effect on the bacterial surface adhesion of these samples.