Bacterial Fermentation Process Research Articles

AKTIVITAS ANTIOKSIDAN METABOLIT SEKUNDER BAKTERI ENDOFIT AKAR TANAMAN Moringa oleifera L (Kelor)

[ANTIOXIDANT ACTIVITY OF SECONDARY METABOLITE FROM ENDOFIT BACTERIA OF Moringa oleifera L (KELOR) ROOTS] The purpose of this research was aims to isolate and measure the ability of antioxidant activity from secondary metabolites produced by endophytic bacteria that grow in the live tissue root of Moringa oleifera L. (kelor). Endophytic bacteria were purified and cultured using a solid Murashige-skoog (MS) medium for 3 days at room temperature. Secondary metabolites were obtained by centrifugation process at a rate of 3000 rpm for 20 minutes. The bacterial fermentation process using Nutrient Broth (NB) medium for 72 hours with a shaker speed at 170 rpm . The suspension supernatant was extracted with a maceration method using 86% ethyl acetate, followed by vacuum rotary evaporator concentration at 40 ° C. The extract antioxidant activity test was performed using the DPPH (1,1-diphenyl-2-picrylhydrazyl) method using a UV-Vis spectrophotometer at 517 nm wavelength and ascorbic acid as standard. The result of DPPH test showed that the antioxidant activity of ethyl acetate extract of endophytic bacterial from root of M. oleifera L root has IC50 value at 315, 396 ppm. Based on these results, it can be concluded that the secondary metabolite extract of endophytic bacterial from M. oleifera L root classified as weak antioxidant (IC50> 250 ppm).

Special Nanoskin-ACT-Biological Membranes from Deep Wounds

Bacterial cellulose (BC) is established as a newest biomaterial, and it can be used for medical and odontology applications. In addition, it has called attention for uses such as membrane for wound care and tissue engineering. In this work, the bacterial cellulose fermentation process is modified by the addition of natural materials before the bacteria are inoculated. In vivo behavior using natural ECM for regenerative medicine is presented and completed wound healing process is 3 months.

521 Enhanced barrier functions and anti-inflammatory effect of cultured coconut extract on human skin

Natural plant oils have been used as a translational alternative to modern medicine. Particularly, virgin coconut oil (VCO) has gained popularity because of its potential benefits in pharmaceutical, nutritional, and cosmetic applications. Cultured coconut extract (CCE) is an alternative end product of VCO, which undergoes a further bacterial fermentation process. This study aimed to investigate the effects of CCE on human skin. We analyzed the expression of skin barrier molecules and collagen after its application on explant human skin. To evaluate the anti-inflammatory properties, the expression of inflammatory markers was analyzed after ultraviolet B (UVB) irradiation. The CCE-treated group showed increased expression of cornified cell envelope components, which contribute to protective barrier functions of the stratum corneum. Furthermore, the expression of inflammatory markers was much lower in the CCE-treated group after exposure to UVB radiation. From such results, we expect an anti-inflammatory effect of CCE against UVB irradiation-induced inflammation. Additionally, the CCE-treated group showed increased collagen and hyaluronan synthase-3 expression. In our study, CCE showed a barrier-enhancing effect and anti-inflammatory properties against ex vivo UVB irradiation-induced inflammation. Due to its beneficial molecular profile, we can expect various clinical implications of CCE in both diseased and healthy skin.

Measurement of abomasal conditions (pH, pressure and temperature) in healthy and diarrheic dairy calves using a wireless ambulatory capsule

This study investigated abomasal luminal parameters in healthy and diarrheic calves by using a wireless ambulatory capsule (WAC). The acetaminophen absorption test (APAT) was used to determine abomasal emptying rate. Four healthy and five diarrheic female Holstein-Friesian calves (age < 14 days) were included in the study. For APAT, calves were fed 2L of milk replacer containing 50mg acetaminophen/kg body weight, and blood samples were taken during a 12-h period afterward. Concomitantly, a WAC in the abomasum continuously measured luminal pH, pressure, and temperature. Five hours post suckling, intraluminal temperature was significantly higher in diarrheic calves than in healthy calves. Abomasal pH and pressure were not significantly different, but intraluminal pressure was always numerically lower in diarrheic calves. During APAT no significant differences in maximum acetaminophen concentrations (Cmax) and time to reach maximum acetaminophen concentration (Tmax) were observed. Nonlinear regression findings revealed a longer acetaminophen half-time (AAP t1/2) in diarrheic calves compared to healthy calves [564 ± 96min vs. 393 ± 84min, respectively; P = 0.04] and lower area under the concentration curve values (e.g., 60min postprandial AUC60 681 ± 244 (µg∙min)/mL vs. 1064 ± 23 (µg∙min)/mL, respectively; P = 0.04). In conclusion, abomasal luminal conditions were different between diarrheic and healthy calves. Significant differences in APAT reflected a delay in abomasal emptying in diarrheic calves. Impaired abomasal movement may induce enhanced bacterial fermentation processes as indicated by a higher abomasal temperature in diarrheic calves, which should be considered in management of their feeding.

Enhanced barrier functions and anti-inflammatory effect of cultured coconut extract on human skin

Natural plant oils have been used as a translational alternative to modern medicine. Particularly, virgin coconut oil (VCO) has gained popularity because of its potential benefits in pharmaceutical, nutritional, and cosmetic applications. Cultured coconut extract (CCE) is an alternative end product of VCO, which undergoes a further bacterial fermentation process. This study aimed to investigate the effects of CCE on human skin. We analyzed the expression of skin barrier molecules and collagens after applying CCE on human explanted skin. To evaluate the anti-inflammatory properties of CCE, the expression of inflammatory markers was analyzed after ultraviolet B (UVB) irradiation. The CCE-treated group showed increased expression of cornified cell envelope components, which contribute to protective barrier functions of the stratum corneum. Further, the expression of inflammatory markers was lower in the CCE-treated group after exposure to UVB radiation. These results suggest an anti-inflammatory effect of CCE against UVB irradiation-induced inflammation. Additionally, the CCE-treated group showed increased collagen and hyaluronan synthase-3 expression. In our study, CCE showed a barrier-enhancing effect and anti-inflammatory properties against ex vivo UVB irradiation-induced inflammation. The promising effect of CCE may be attributed to its high levels of polyphenols and fatty acid components.

Isolation and Characterization of Chitin Nanowhiskers from Fermented Tiger Prawn Waste

The objective of this study is to produce chitin nanowhiskers (CNW) from bacterial fermentation of tiger prawn waste. For this purpose, chitin was first extracted from tiger prawn waste using bacterial fermentation process followed by isolation of CNW using acid hydrolysis process. The isolated CNW from fermented tiger prawn waste (FCNW) were investigated using Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), atomic force microscopy (AFM), x-ray diffractometer (X-RD) and thermogravimetric analysis (TGA). FTIR spectra analysis indicates that the acid hydrolysis of chitin did not altered the chemical structure of isolated FCNW. TEM analysis revealed that the produced FCNW displayed a nanoscale structure with an average length and width of 100 and 10 nm. AFM images of FCNW indicate the presence of spindle-like features. The X-RD analysis revealed that the acid hydrolysis process enhanced the crystallinity of FCNW from 20 to 46 % compared to chitin due to the removal of the amorphous region. The TGA results revealed that the FCNW is more thermally stable than fermented chitin (FC) and CNW from commercial chitin. The relatively good thermal stability of FCNW shows its suitability in a range of applications such as reinforcing fillers in green nanocomposites.

Surface physical chemistry properties in coated bacterial cellulose membranes with calcium phosphate

Bacterial cellulose has become established as a new biomaterial, and it can be used for medical applications. In addition, it has called attention due to the increasing interest in tissue engineering materials for wound care. In this work, the bacterial cellulose fermentation process was modified by the addition of chondroitin sulfate to the culture medium before the inoculation of the bacteria. The biomimetic process with heterogeneous calcium phosphate precipitation of biological interest was studied for the guided regeneration purposes on bacterial cellulose. FTIR results showed the incorporation of the chondroitin sulfate in the bacterial cellulose, SEM images confirmed the deposition of the calcium phosphate on the bacterial cellulose surface, XPS analysis showed a selective chemical group influences which change calcium phosphate deposition, besides, the calcium phosphate phase with different Ca/P ratios on bacterial cellulose surface influences wettability. XTT results concluded that these materials did not affect significantly in the cell viability, being non-cytotoxic. Thus, it was produced one biomaterial with the surface charge changes for calcium phosphate deposition, besides different wettability which builds new membranes for Guided Tissue Regeneration.

Economic comparison of a continuous ABE fermentation with and without the integration of an in situ vacuum separation unit

The development of renewable fuels has achieved enormous progress motivated by the rapid decline in petroleum resources and their oscillating price. After a wave of interest in bioethanol and biodiesel, biobutanol has been intensely investigated over the last decade and is considered a suitable renewable fuel. Biobutanol is most often produced through the Acetone‐Butanol‐Ethanol (ABE) bacterial fermentation process. This process is plagued, however, with high recovery costs because of the low final butanol concentration (∼0.01 g/g, 1 wt %) due to product inhibition on the microorganisms. To partly alleviate this problem and increase productivity, in situ butanol recovery techniques from the fermentation broth were proposed. These integrated methods increase the final concentration of butanol, and as a result improve the separation efficiency of the process. This paper compares the economic feasibility of a large‐scale continuous ABE fermentation process with and without integrating a vacuum separation unit. It is illustrated that based on the current market price of butanol, only the integrated fermentation process is economically feasible for discount rates up to ∼45 %. Adding an in situ butanol recovery technique made this process profitable compared to the conventional fermentation process analyzed under two different scenarios: constant fermenter volume and constant butanol production rate. The net present values (NPV) of the conventional process were found to be negative for both scenarios, whereas the integrated vacuum process had a NPV of US$ 87 000 000 at the end of 10 years of operation based on a discount rate of 10 %.

Study of Nanoskin ECM-Bacterial Cellulose Wound Healing/United Arab Emirates

Natural extracellular matrices (ECMs) perform the tasks necessary for tissue formation, maintenance, regulation and function, providing a powerful means of controlling the biological performance of regenerative materials. In addition, biomedical materials have claimed attention because of the increased interest in tissue engineering materials for wound care and regenerative medicine. Moreover, the nanostructure and morphological similarities with collagen make BC attractive for cell immobilization, cell support and Natural Extracellular Matrix (ECM) Scaffolds. In this work, we present the extracellular matrix (ECM) using the bacterial cellulose (Nanoskin®) which regulates cell behavior by influencing cell proliferation, survival, shape, migration and differentiation. Bacterial cellulose fermentation process is modified before the bacteria are inoculated for mimicking ECM to cells support and built new local material for wound healing. Chemical groups influences and thermal behavior in bacterial cellulose were analyzed using transmission infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA), respectively. Besides, In vivo analysis was evaluated with clinical study at Sharjah Kuwait Hospital.

Nanoskin Extracellular Matrix Modified with Hyaluronic Acid and Chondroitin Sulfate for Regenerative Medicine Applications

Natural extracellular matrices (ECMs) perform the tasks necessary for tissue formation, maintenance, regulation and function, providing a powerful means of controlling the biological performance of regenerative materials. The extracellular matrix represents Mother Nature's biologic scaffold. The ECM is rich in structural and functional proteins that not only maintain the health of differentiated tissues, but support the host response to tissue injury. In this work, we present the extracellular matrix (ECM) using the bacterial cellulose (Nanoskin®) which regulates cell behavior by influencing cell proliferation, survival, shape, migration and differentiation. Bacterial cellulose fermentation process is modified by the addition of vegetal stem cell to the culture medium and natural materials (hyaluronic acid and chondroitin sulfate) before the bacteria are inoculated for mimicking ECM to cells support and built new skin formation.

Natural ECM-Bacterial Cellulose Wound Healing—Dubai Study

Bacterial cellulose (BC) can be used in wide area of applied scientific, especially for tissue regeneration and regenerative medicine, lately, bacterial cellulose mats are used in the treatment of skin conditions such as burns and ulcers, because of the morphology of fibrous biopolymers serving as a support for cell proliferation, its pores allow gas exchange between the organism and the environment. Moreover, the nanostructure and morphological similarities with collagen make BC attractive for cell immobilization, cell support and Natural Extracellular Matrix (ECM) Scaffolds. In this scope, Natural ECM is the ideal biological scaffold since it contains all the components of the tissue. The development of mimicking biomaterials and hybrid biomaterial can further advance directed cellular differentiation without specific induction. The extracellular matrix (ECM) contains several signals that are received by cell surface receptors and contribute to cell adhesion and cell fate which control cellular activities such as proliferation, migration and differentiation. As such, regenerative medicine studies often rely on mimicking the natural ECM to promote the formation of new tissue by host cells, and characterization of natural ECM components is vital for the development of new biomimetic approaches. In this work, the bacterial cellulose fermentation process is modified by the addition of vegetal stem cell to the culture medium and natural materials before the bacteria are inoculated. In vivo behavior using natural ECM for regenerative medicine is presented.

Novel Chemically Modified Bacterial Cellulose Nanocomposite as Potential Biomaterial for Stem Cell Therapy Applications

Bacterial cellulose (BC) has become established as a remarkably versatile biomaterial and can be used in a wide variety of applied scientific applications, especially for medical devices. In this work, the bacterial cellulose fermentation process is modified by the addition of hyaluronic acid and gelatin (1% w/w) to the culture medium before the bacteria is inoculated. Hyaluronic acid and gelatin influence in bacterial cellulose was analyzed using Transmission Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM). Adhesion and viability studies with human dental pulp stem cells using natural bacterial cellulose/hyaluronic acid as scaffolds for regenerative medicine are presented for the first time in this work. MTT viability assays show higher cell adhesion in bacterial cellulose/gelatin and bacterial cellulose/ hyaluronic acid scaffolds over time with differences due to fiber agglomeration in bacterial cellulose/gelatin. Confocal microscopy images showed that the cell were adhered and well distributed within the fibers in both types of scaffolds.

Pandemism of swine flu and its prospective drug therapy

Swine flu is a respiratory disease caused by influenza A H1N1 virus. The current pandemic of swine flu is most probably due to a mutation-more specifically, a re-assortment of four known strains of influenza A virus subtype H1N1. Antigenic variation of influenza viruses while circulating in the population is an important factor leading to difficulties in controlling influenza by vaccination. Due to the global effect of swine flu and its effect on humans, extensive investigations are being undertaken. In this context, Tamiflu is the only available drug used in the prophylaxis of this disease and is made from the compound shikimic acid. Due to the sudden increase in the demand of shikimic acid, its price has increased greatly. Thus, it is necessary to find an alternative approach for the treatment of swine flu. This review presents the overall information of swine flu, beginning from its emergence to the prevention and treatment of the disease, with a major emphasis on the alternative approach (bacterial fermentation process) for the treatment of swine flu. The alternative approach for the treatment of swine flu includes the production of shikimic acid from a fermentation process and it can be produced in large quantities without any time limitations.

Influence of Symbioses Culture between Microorganisms/Yeast Strains on Cellulose Synthesis

Bacterial cellulose (BC), which is produced by some strains of the bacterial genera Acetobacter, represents a potential alternative to plant-derived cellulose. Due to its high water-holding capacity, high crystallinity, high tensile strength and fine web-like network structure, which means that it can be formed into any size or shape, BC is being used as a promising nanofiber biomaterial. The bacterial cellulose fermentation process is achieved by using the sugar as carbohydrate source. Different carbon sources can be used for the cellulose synthesis, namely glucose, fructose and cane sugar. Results of this process would be nanobiocellulose biomass.In order to improve cellulose production, determine the purity and some structural features of the cellulose from this strain; it was isolated and identified the microorganisms from kombucha and their ability to cellulose biosynthesis. It was found that the microorganisms Gluconacetobacter intermedius is the best for cellulose production with higher cellulose levels (14.63g/L) in static culture conditions. These results indicating the G. intermedius strain from Kombucha has industrial and commercial potential for cellulose production

Mechanistic studies on the ring-opening polymerisation of D,L-lactide with zinc guanidine complexes

The increasing shortage of resources has enforced the search for production techniques for biodegradable polymers made of renewable raw materials. An example is polylactide (PLA), an aliphatic polyester, which can be obtained by the metal-catalysed ring-opening polymerisation (ROP) of lactide (Fig. ​(Fig.1).1). The monomer for PLA production is available from corn or sugar beets by a bacterial fermentation process in few steps [1] The PLA can be either recycled or composted after use, making the use CO2-emission-neutral. Most large-scale processes are based on the use of tin compounds as initiators [2]. However, for use in food packaging or similar applications, heavy metals are undesirable [3]. Figure 1 ROP of D,L-lactide In order to substitute heavy-metal-based catalyst systems, especially zinc guanidine complexes are suited for the polymerisation due to their polymerisation activity and non-toxicity [4]. The investigations on the catalytic potential in the bulk polymerisation of lactide have shown that these compounds are able to act as initiators for lactide polymerisation with only few exceptions. Polylactides with molecular weights (MW) between 20.000 and 170.000 g/mol could be obtained. As the polymerisation activity depends strongly on the ligand structure (spacer, guanidine and amine unit), DFT studies on the complex properties have been performed [5]. A correlation between calculated partial charges on the zinc and the guanidine N atoms and the catalytic activity has been found. The guanidine is supposed to open nucleophilically the lactide ring and supersede the presence of alkoxides which is traditionally required. This crucial step in the ROP has been analysed by DFT and a mecha-nism will be presented. The deeper understanding of the catalytic transition state will enable a more efficient access to catalyst design. Figure ​Figure22. Figure 2 General motif for hybridquanidines

Current theories on the pathophysiology of equine laminitis – the link between the gut and foot

Equine laminitis is a painful debilitating condition that seems to be a product of domestication of horses associated with the practice of feeding carbohydrate rich pasture in excess of requirements, particularly in ponies. We have been working on the hypothesis that caecal bacteria, when presented with carbohydrate in large quantities tend to ferment this. Fermentation by gram positive bacteria is associated with a fall in pH of the caecal liquor. As a means of providing themselves with increased intracellular buffering capacity, these bacteria produce amino acid decarboxylase enzymes which convert amino acids present in the caecal fluid into primary amines which can act as intracellular buffers. The reason we thought this might be the case was that foods formed by bacterial fermentation processes (e.g. cheese and wines) have a high content of amines. Primary amines derived from some amino acids have vasoactive properties. In the 1960s it was recognised that human patients taking drugs that inhibit the metabolism of amines (monoamine oxidase inhibitors) could suffer from severe hypertensive crises if they ate cheese or drank red wines with high tyramine content. This so called ‘cheese effect’ is the result of the indirect sympathomimetic effect of tyramine which displaces norepinephrine from sympathetic nerve endings leading to a rise in blood pressure. Monoamine oxidase enzymes present in the epithelial cells lining the gut wall and in liver cells normally protect individuals from suffering these hypertensive crises. We wondered whether amines released from the gut in response to ingestion of excessive amounts of carbohydrate might lead to a similar reaction in ponies whereby dietary amines caused vasoconstriction of the digital vasculature, leading to the prodromal phases of laminitis.

Biological characterization of Sorona polymer from corn-derived 1,3-propanediol

The Sorona family of polymers, based on corn-derived 1,3-propanediol, have recently been developed as novel bio-based materials for use in plastics, films, and fibers. In the present study, Sorona polymers were investigated for their effects on cell survival and inflammatory cell activation using in vitro mouse cell cultures. Cytotoxicity of Sorona polymers was evaluated by placing material samples in direct contact with 3T3 fibroblast cells. Both Sorona plastic and Sorona films were non-cytotoxic to fibroblasts. The inflammatory potential of Sorona samples was evaluated by exposing J774 macrophage cells to material samples, and measuring TNF-alpha release from macrophages. Sorona plastic and Sorona films did not elicit inflammatory TNF-alpha release from macrophages. These results indicate that Sorona polymers are non-cytotoxic and non-inflammatory. While the 1,3-propanediol component of Sorona 3GT is manufactured in a bacterial fermentation process, the absence of an inflammatory response to Sorona film and Sorona plastic is highly encouraging. The results are significant for the design of materials that utilize bio-based polymers.

Butyrate in the treatment of ulcerative colitis

Abstract Butyrate, a substance produced in the colon via bacterial fermentation processes, constitutes an important source of energy for colonic mucosa cells. It also plays an important role in the regulation of intraluminal homeostasis, modulation of inflammation, control of cellular proliferation and differentiation, and repair of mucosal lesions. Butyrate has been studied in clinical applications, particularly inflammatory bowel diseases of the colon. While available data are not entirely conclusive, this substance appears to have a useful therapeutic role complementary to that of standard drugs. A body of experimental data also suggests that this short-chain fatty acid may exert preventive action against colorectal cancer, but for the moment this is still a hypothesis that remains to be verified. More generally, butyrate has been shown to be useful in certain types of diarrhoea, particularly chronic forms, by promoting absorption of water and electrolytes.

Functional Analyses of the Malolactic Wine Yeast ML01

Deacidification of grape musts and wines is important for the production of well-balanced wines. The bacterial malolactic fermentation (MLF) process is unreliable and stuck MLF often leads to spoilage of wines and the production of biogenic amines. The genetically engineered wine yeast, ML01, is a Prise de Mousse strain that contains the malate transport gene (<i>mae1</i>) from <i>Schizosaccharomyces pombe</i> and the malolactic gene (<i>mleA</i>) from <i>Oenococcus oeni</i>, stably integrated into the genome at the <i>URA3</i> locus. Both genes were isolated from wine-related microorganisms and are expressed under control of the <i>Saccharomyces cerevisiae PGK1</i> promoter and terminator sequences. ML01 is capable of decarboxylating up to 9.2 g/L of malate to equimolar amounts of lactate in Chardonnay grape must during the alcoholic fermentation. ML01 contains no antibiotic resistance marker genes or vector DNA sequences. The presence of the malolactic cassette in the genome does not affect growth, ethanol production, fermentation kinetics, or metabolism of ML01. Wines produced by the ML01 yeast have lower volatile acidity and improved color properties than wines produced with the parental yeast and a bacterial MLF. Analysis of the volatile compounds, sensory analyses, and industrial production of wine indicate that ML01 is suitable for the commercial production of quality wine.

Bacterial Symbionts: Prospects for the Sustainable Production of Invertebrate-Derived Pharmaceuticals

Invertebrate animals, such as sponges, tunicates and bryozoans, are among the most important sources of biomedically relevant natural products. However, as these animals generally contain only low quantities of the compounds, further pharmacological development is in most cases difficult. There is increasing evidence that many metabolites, in particular polyketides and nonribosomally synthesized peptides, are not produced by the animals themselves but by associated bacterial symbionts. This symbiont hypothesis currently attracts considerable interest, since it implicates that animal-independent production systems based on bacterial fermentation processes could be created. This review gives an overview about recent developments in the research on natural product symbiosis. Different techniques will be discussed that have been employed to pinpoint the actual producer. Since bacterial symbionts are highly fastidious and have been generally resistant to cultivation attempts, emphasis will be laid on culture-independent strategies, such as cell separation approaches and the cloning of biosynthetic genes. These strategies have provided insights into possible sources of several natural products, e.g. the bryostatins, pederin, the onnamides, swinholide A and theopalauamide. Finally, potential techniques for the generation of renewable supplies of symbiont-derived drug candidates will be discussed. Cultivation approaches and the heterologous expression of cloned biosynthesis genes from uncultured symbionts could in future provide access to several important marine drug candidates, including bryostatin 1, halichondrin or ET-743.