Under the background of economic globalization and cross-cultural management, this paper proposes hypothesis and model of affective identification motivation mechanism in Sino-Japanese bi-cultural teleworking (BCT) teams, based on ample resources of literature and system rational analysis thinking. This paper firstly studies the working process of Sino-Japanese BCT teams by management experimental methods. And then the experiment data and proposed hypothesis are analyzed and verified statically. Finally, a conclusion is obtained that in Sino-Japanese BCT teams, team's affective support, work attitude identification, cultural affective identification can enhance team identification, moderated by team members’ perception level.