Backing Layer Research Articles

Layer-by-layer microneedle patch with antibacterial and antioxidant dual activities for accelerating bacterial-infected wound healing

Bacterial-infected wound healing has always been a huge challenge to humans. Owing to the appearance of antibiotic resistance, there is an emergency need to design antibiotic-free wound dressings to treat such wounds. Herein, a novel antibiotic-free microneedle patch was designed, which its backing layer with antioxidant effect was coated with sodium carboxymethyl cellulose, 2-O-α-D-glucopyranosyl-L-ascorbic acid (GLAA), and 2-hydroxypropyltrimethyl ammonium chloride chitosan through electrostatic interaction based on layer-by-layer self-assembly technique, and its tips consisted of gelatin and tannic acid (TA) via hydrogen bonding interaction (CGH/GTA MN patch). The obtained CGH/GTA MN patch could effectively puncture the skin, and exhibit properties of pH-responsive TA and GLAA release. In vitro experiments showed that the obtained CGH/GTA MN patch has excellent antioxidative (scavenging DPPH efficacy is above 80 %, and scavenging ABTS efficiency reaches about 100 %), antibacterial (antibacterial rates of nearly 100 % for both Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli)), biodegradable, and biocompatible properties. In the S. aureus-infected rat wounds, the CGH/GTA MN patch could efficiently accelerate infected-wound healing by eliminating S. aureus infection, inhibiting inflammation, promoting angiogenesis, and accelerating epidermal regeneration. Thus, this study will provide a promising strategy to heal bacterial-infected wounds.

Ballistic performance of double-layered plates Q235 and 45 steel subjected to impact by projectiles of middle strength

The ballistic resistance behavior of double-layer steel plates incorporated with different materials is experimentally and numerically investigated by using blunt- and ogive-nosed projectiles of middle strength. These materials are Q235 steel (low strength and high ductility) and 45 steel (high strength and low ductility). Experimental results showed that the ballistic resistance is better for the double-layer plates with the front layer of low ductility and high strength and the back layer of high ductility and low strength material than that of the opposite layer order impacted by ogive-nosed projectiles, but the situation is opposite for blunt-nosed projectiles. The ballistic limit of H + A (753.85 m/s) is increased by 29.5% when compared to that of A + H (582.19 m/s) for ogive-nosed projectiles of 45 steel. In the case of blunt projectiles, a 2.8% decrease in the ballistic limit of middle-strength projectile. Ogive-nosed projectiles are more sensitive than blunt-nosed projectiles when it comes to the effect of the layer order of the double-layer plates. Moreover, the ballistic limit velocities of blunt-nosed projectiles are obviously bigger than that of ogive-nosed projectiles for the double-layer plates of the front layer of low strength, while the situation is opposite for the double-layer plates of the front layer of high strength. Furthermore, the projectiles lose some mass and length after impact. The deformation of projectiles is also concerning the nose shape and velocity of projectiles, as well as the layer order of the double-layer plates. Finally, numerical simulation results are obtained and compared using the ABAQUS subroutine VUMAT-modified Johnson-Cook material model. And these results fairly agree with the experiment.

The Optimization of Pressure-Assisted Microsyringe (PAM) 3D Printing Parameters for the Development of Sustainable Starch-Based Patches.

The aim of this work was to develop sustainable patches for wound application, using the biopolymer starch, created using a low-cost 3D printing PAM device. The composition of a starch gel was optimized for PAM extrusion: corn starch 10% w/w, β-glucan water suspension (filler, 1% w/w), glycerol (plasticizer, 29% w/w), and water 60% w/w. The most suitable 3D printing parameters were optimized as well (nozzle size 0.8 mm, layer height 0.2 mm, infill 100%, volumetric flow rate 3.02 mm3/s, and print speed 15 mm/s). The suitable conditions for post-printing drying were set at 37 °C for 24 h. The obtained patch was homogenous but with low mechanical resistance. To solve this problem, the starch gel was extruded over an alginate support, which, after drying, becomes an integral part of the product, constituting the backing layer of the final formulation. This approach significantly improved the physicochemical and post-printing properties of the final bilayer patch, showing suitable mechanical properties such as elastic modulus (3.80 ± 0.82 MPa), strength (0.92 ± 0.08 MPa), and deformation at break (50 ± 1%). The obtained results suggest the possibility of low-cost production of patches for wound treatment by additive manufacturing technology.

Spidroin-based multifunctional microneedles with controlled drug release for efficient wound management

Wound healing remains a serious challenge in the biomedical field due to its complex physiological processes. To address this issue, microneedle patches are proposed as an effective strategy for wound management. Herein, we propose novel breathable microneedle patches containing therapeutic drugs and functional materials for wound healing, which are fabricated using extrusion 3D printing technology. The microneedle patch is composed of needle tips and a breathable scaffold backing layer made of spidroin hydrogel, polyurethane, and aloe vera gel, providing excellent biocompatibility and self-healing ability. Furthermore, eutectic Galium-Indium (EGaIn) was incorporated into the dressing, enabling the near-infrared light response function. The highly efficient photothermal conversion allowed for superfast self-healing in seconds. The microneedle dressing was sensitive enough to monitor large-scale movements at different joints and small-scale motions of the throat during swallowing. Combining biocompatible microneedles with a temperature-responsive hydrogel (pNIPAM), stable controllable drug delivery is achieved using near-infrared (NIR) light. The stable photothermal performance enabled the device to release a controlled dose of the drug over multiple light triggers. With NIR-triggered pulse drug release, VEGF-mediated treatment on wound healing was significantly improved in mouse models by accelerating tissue repair and reducing the inflammatory response.

Preload-Induced Switchable Adhesion.

Animals with robust attachment abilities commonly exhibit stable attachment and convenient detachment. However, achieving an efficient attachment-detachment function in bioinspired adhesives is challenging owing to the complexity and delay of actuators. In this study, a class of multilayer adhesives (MAs) comprising backing, middle, and bottom layers is proposed to realize rapid switching by only adjusting the preload. At low preload, the MAs maintain intimate contact with the substrate. By contrast, a sufficiently large preload results in significant deformation of the middle layer, causing underside buckling and reducing adhesion. By optimizing the structural parameters of the MAs, a high switching ratio (up to 136×) can be achieved under different preloads. Furthermore, the design of the MAs incorporates a film-terminated structure, which prevents the embedding of dirt particles, simplifies cleaning, and maintains the separation and uprightness of the microstructures. Consequently, the MAs demonstrate practical potential for simple and efficient transportation applications, as they achieve switchable adhesion through their structure, exhibiting a high switching ratio and fast switching.

Exploring the Potential of Pure Germanium Kesterite for a 2T Kesterite/Silicon Tandem Solar Cell: A Simulation Study.

Recently, the development of tandem devices has become one of the main strategies for further improving the efficiency of photovoltaic modules. In this regard, combining well-established Si technology with thin film technology is one of the most promising approaches. However, this imposes several limitations on such thin film technology, such as low prices, the absence of scarce or toxic elements, the possibility to tune optical properties and long lifetime stability. Therefore, to show the potential of kesterite/silicon tandems, in this work, a 2 terminal (2T) structure using pure germanium kesterite was simulated with combined SCAPS and transfer matrix methods. To explore the impact of individual modifications, a stepwise approach was adopted to improve the kesterite. For the bottom sub cell, a state-of-the-art silicon PERC cell was used with an efficiency of 24%. As a final result, 19.56% efficiency was obtained for the standalone top kesterite solar cell and 28.6% for the tandem device, exceeding standalone silicon efficiency by 4.6% and justifying a new method for improvement. The improvement observed could be attributed primarily to the enhanced effective lifetime, optimized base doping, and mitigated recombination at both the back and top layers of the CZGSSe absorber. Finally, colorimetric analysis showed that color purity for such tandem structure was low, and hues were limited to the predominant colors, which were reddish, yellowish, and purple in an anti-reflective coating (ARC) thickness range of 20-300 nm. The sensitivity of color variation for the whole ARC thickness range to electrical parameters was minimal: efficiency was obtained ranging from 28.05% to 28.63%.

Timing, Energy, and Spatial Characterization of Highly Sampled Monolithic PET Detectors with Different Thicknesses.

The HyPET project proposes a hybrid dedicated TOF-PET for prostate imaging, with pixelated detector blocks in the front layer and monolithic blocks in the back layer. In this work, four detector configurations have been experimentally evaluated for the rear detector layer. The detector configuration consists of LYSO monolithic blocks with the same size (25.4 mm × 25.4 mm) but different thicknesses (5, 7.5, 10, and 15 mm) coupled to the same SiPM array. Each detector configuration has been experimentally characterized in terms of time, energy and spatial resolution by scanning the crystal surface using a fan beam in steps of 0.25 mm. Regarding spatial resolution, the interaction position was estimated using a Neural Network technique. All resolutions except energy, which remains nearly constant at 17% for all cases, show better values for the 5 mm detector thickness. We have achieved spatial resolution values of FWHM of 1.02 ± 0.10, 1.19 ± 0.13, 1.53 ± 0.17, 2.33 ± 0.55 mm, for the 5, 7.5, 10, and 15 mm blocks, respectively. The detector time resolution obtained was 275 ± 26, 291 ± 21, 344 ± 48, and 433 ± 45 ps respectively, using the energy weighted average method for the time stamps.

Enhancement Quality of Particleboard from Oil Palm Trunk and Mahogany Sawdust with Layering of Talang Bamboo (Schizostachyum brachycladumc)

Oil Palm Trunk (OPT) is a natural fiber used as an alternative material in wood-derived products such as particleboard. The OPT has low characteristics when using complete trunk, it is expected that its application would be a solution to reduce this plantation waste. In this investigation, the raw material of OPT particles was mixed with Mahogany, and Talang bamboo lamina to improve the physical and mechanical properties of the particleboard. This study aims to evaluate the addition of particleboard with various layerings of Talang Bamboo. The particleboard has three layers, with Talang bamboo (bamboo outer and inner part) on the face and back layers. There are six types of particleboard made: control, bamboo inner layering, bamboo outer layering, two bamboo inner layerings, two bamboo outer layerings, and a mixture of inner and outer bamboo layerings. The physical and mechanical properties of particleboard were testing according to JIS A 5908-2003 standard. The results of the physical properties showed that the density was 0.56-0.70 g/cm3, moisture content was 3.03-4.22%, water absorption was 52.01-87.15%, and thickness swelling was 11.62-24.68%. The mechanical properties showed MOE values was 9975.76-84246.63 kg/cm2, MOR was 81.64-670.77 kg/cm2, and IB was 0.91-3.38 kg/cm2. The physical and mechanical properties of particleboard met the JIS A 5908-2003 standard. According to this investigation's findings, Layering with the outer part of the Talang bamboo on the face and back of the particleboard is the best

A terahertz wideband linear-to-circular polarization converter based on meandering-structured metasurface

In the terahertz (THz) band, this paper proposes a transmissive linear-to-circular (LTC) polarization converter based on a bilayer metallic meandering-structured metasurface. In this design, the LTC polarization conversion is achieved by utilizing the meandering structure, and the bandwidth is greatly expanded by inserting a wire grid into the y direction of the back metal layer. Furthermore, this metasurface converter is characterized by its simple structure and ultra-thin thickness. In detail, the axial ratio of the proposed converter is less than 3 dB in the frequency range of 0.4–1.21 THz signifying 100% relative bandwidth. Meanwhile, the emulation polarization conversion efficiency exceeds 60% in the frequency range of 0.68–1.21 THz, and the experimental results are in general agreement with the simulation ones. The proposed converter provides a new idea for the LTC polarization conversion in a wide THz band.

Development and Characterization of Gel-Based Buccoadhesive Bilayer Formulation of Nifedipine.

A promising controlled drug delivery system has been developed based on polymeric buccoadhesive bilayered formulation that uses a drug-free backing layer and a polymeric hydrophilic gel buccoadhesive core layer containing nifedipine. The DSC thermogravimetric analysis confirms the drug's entrapment in the gel layer and reveals no evidence of a potential interaction. Various ratios of bioadhesive polymers, including HPMC K100, PVP K30, SCMC, and CP 934, were combined with EC as an impermeable backing layer to ensure unidirectional drug release towards the buccal mucosa. The polymeric compositions of hydrophilic gel-natured HPMC, SCMC, and CP formed a matrix layer by surrounding the core nifedipine during compression. Preformulation studies were performed for all of the ingredients in order to evaluate their physical and flow characteristics. Ex vivo buccoadhesive strength, surface pH, swelling index, in vitro and in vivo drug release, and ex vivo permeation investigations were performed to evaluate the produced gel-based system. Rapid temperature variations had no appreciable impact on the substance's physical properties, pharmacological content, or buccoadhesive strength during stability testing using actual human saliva. It was clear from a histological examination of the ex vivo mucosa that the developed system did not cause any irritation or inflammation at the site of administration. The formulation NT5 was the best one, with a correlation coefficient of 0.9966. The in vitro and in vivo drug release profiles were well correlated, and they mimic the in vitro drug release pattern via the biological membrane. Thus, the developed gel-based formulation was found to be novel, stable, and useful for the targeted delivery of nifedipine.

Multimaterial Topology Optimization of Adhesive Backing Layers via J-Integral and Strain Energy Minimizations

Abstract Strong adhesives often rely on reduced stress concentrations obtained via specific functional grading of material properties. This can be seen in many examples in nature and engineering. Basic design principles have been formulated based on parametric optimization, but a general design tool is still missing. We propose here the use of topology optimization to achieve optimal stiffness distribution in a multimaterial adhesive backing layer, reducing stress concentration at selected (crack tip) locations. The method involves the minimization of a linear combination of (i) the J-integral around the crack tip and (ii) the strain energy of the structure. This combination is due to the compromise between numerical stability and accuracy of the method, where (i) alone is numerically unstable and (ii) alone cannot eliminate the crack tip stress singularity. We analyze three cases in plane strain conditions, namely, (1) double-edged crack and (2) center crack, in tension, as well as (3) edge crack under shear. Each case evidences a different optimal topology with (1) and (2) providing similar results. The optimal topology allocates stiffness in regions that are far away from the crack tip, and the allocation of softer materials over stiffer ones produces a sophisticated structural hierarchy. To test our solutions, we plot the contact stress distribution across the interface. In all observed cases, we eliminate the stress singularity at the crack tip, albeit generating (mild) stress concentrations in other locations. The optimal topologies are tested to be independent of the crack size. Our method ultimately provides the robust design of flaw tolerant adhesives where the crack location is known.

An Integrated Therapeutic and Preventive Nanozyme-Based Microneedle for Biofilm-Infected Diabetic Wound Healing.

The healing of biofilm-infected diabetic wounds characterized by a deteriorative tissue microenvironment represents a substantial clinical challenge. Current treatments remain unsatisfactory due to the limited antibiofilm efficacy caused by weak tissue and biofilm permeability of drugs and the risk of reinfection during the healing process. To address these issues, an integrated therapeutic and preventive nanozyme-based microneedle (denoted as Fe2 C/GOx@MNs) is engineered. The dissolvable tips with enough mechanical strength can deliver and rapidly release Fe2 C nanoparticles (NPs)/glucose oxidase (GOx) in the biofilm active regions, enhancing tissue and biofilm permeability of Fe2 C NPs/GOx, ultimately achieving highly efficient biofilm elimination. Meanwhile, the chitosan backing layer can not only act as an excellent physical barrier between the wound bed and the external environment, but also prevent the bacterial reinvasion during wound healing with its superior antibacterial property. Significantly, the biofilm elimination and reinfection prevention abilities of Fe2 C/GOx@MNs on wound healing are proved on methicillin-resistant Staphylococcus aureus-biofilm-infected diabetic mouse model with full-thickness wound. Together, these results demonstrate the promising clinical application of Fe2 C/GOx@MNs in biofilm-infected wound healing.

Design and Performance of Layered Heterostructure Composite Material System for Protective Armors.

A new layered heterostructure composite material system (TC4 as front layer and 2024Al alloy as back layer) was developed and analyzed for its design and performance in terms of an enhanced absorption capability and anti-penetration behavior. The Florence model for energy absorption was modified, so that it can be utilized for the layered heterostructure composite material system with more efficacy. Numerical simulation through Ls-Dyna validated the analytical model findings regarding the energy absorption of the system and both were in good agreement. Results showed that two ductile materials with diverse properties, the hardness gradient and varied layer thickness joined together, specifically behaved like a unified structure and exhibited elastic collision after slight bending, which is possibly due to the decreased yield strength of the front layer and increased yield strength of the second layer. To validate the analytical and numerical findings, the samples of the layered heterostructure composite material system were subjected to a SHPB (Split Hopkinson pressure bar) compression test. The deformation behavior was analyzed in the context of the strain energy density and stain rate sensitivity parameter at different strain rates. The encouraging results proposed that two ductile materials with a hardness gradient can be used as an alternate structure instead of a brittle-ductile combination in a layered structure.

Multifunctional Covalent Organic Framework-Based Microneedle Patch for Melanoma Treatment.

Melanoma is resistant to conventional chemotherapy and radiotherapy. Therefore, it is essential to develop a targeted, low-toxic, and minimally invasive treatment. Here, DTIC/ICG-Fe3O4@TpBD BSP/HA microneedles (MNs) were designed and fabricated, which can enhance targeting to melanoma and perform photothermal therapy (PTT) and chemotherapy simultaneously to synergistically exert anticancer effects. The system consisted of magnetic nanoparticles (DTIC/ICG-Fe3O4@TpBD), dissoluble matrix (Bletilla polysaccharide (BSP)/hyaluronic acid (HA)), and a polyvinyl alcohol backing layer. Due to the good magnetic responsiveness of Fe3O4@TpBD, dacarbazine (DTIC) and indocyanine green (ICG) can be better targeted to the tumor tissue and improve the therapeutic effect. BSP and HA have good biocompatibility and transdermal ability, so that the MNs can completely penetrate the tumor tissue, be dissolved by the interstitial fluid, and release DTIC and ICG. Under near-infrared (NIR) light irradiation, ICG converts light energy into thermal energy and induces ablation of B16-OVA melanoma cells. In vivo results showed that DTIC/ICG-Fe3O4@TpBD BSP/HA MNs combined with chemotherapy and PTT could effectively inhibit the growth of melanoma without tumor recurrence or significant weight loss in mice. Therefore, DTIC/ICG-Fe3O4@TpBD BSP/HA MNs are expected to provide new ideas and therapeutic approaches for the clinical treatment of melanoma.

Restoration as reconnection: A relational approach to urban stream repair

AbstractUrban stream environments have been significantly altered through processes of colonisation and urbanisation. In Te Whanganui‐a‐Tara Wellington, Aotearoa New Zealand, there is growing interest in peeling back layers of the city to reconnect with waterways. More‐than‐human geographies can play a critical role in contributing to these efforts, guiding understandings of what it means to restore and live alongside urban streams. In our case study of the Waimapihi Stream, we explore one community's ideas and practices of restoration and how they envision a thriving place through the notion of stream daylighting. The Waimapihi shows us that restoration activities are both product and process of co‐creating an ontologically plural space for the renegotiation of what stream restoration means.

An inflammation-responsive double-layer microneedle patch for recurrent atopic dermatitis therapy

Seeking a potent therapeutic strategy for alleviating atopic dermatitis (AD) attack and preventing its recurrence is highly desired but remains challenging in clinical practice. Here, we propose an inflammation-responsive double-layer microneedle (IDMN) patch in situ delivering VD3 for recurrent AD therapy. IDMN comprises the backing layer part and the double-layer microneedle part, in which the inner layer is gelatin methacryloyl (GelMA) loaded with VD3 while the outer layer is composed of hyaluronic acid (HA). Introduction of the HA backing layer and outer layer around the GelMA tips can not only provide sufficient mechanical strength to penetrate into hardened AD skin with minimal invasiveness, but also exert a strong moisturizing effect after being rapidly dissolved. The inner layer of GelMA is degraded by the matrix metalloproteinase (MMP) in a dose dependent manner, which is secreted according to the disease progression of AD. The responsive degradation of GelMA tips result in corresponding release of VD3 to treat AD, triggering negative feedback against GelMA degradation. The IDMN administration on AD-bearing mice reveals efficient “curing” performances (including suppress erythema, scaling and lichenification, reduce epidermal thickness, inhibit mast cells infiltration, and down-regulate inflammatory factor secretion), which are basically realized through synergistic effect of the released VD3 and the dissolved HA molecules. Importantly, the residual tips of IDMN with VD3 are retained in the skin after the first AD relief, showing promising “warning” ability to inhibit the recurrence of AD. Hence, the developed IDMN patch is expected to be one of the excellent candidates for AD therapy and other relapsing diseases in clinical fields.

Investigating the effect of ceramic-polyurea-aluminum layers on ballistic performance of composite target

In this research, the ballistic performance of ceramic-polyurea-aluminum composite targets under the impact of flat-nose projectile was investigated for different thicknesses. The relevant experiments were designed based on the thickness of the layers and the effect of their configuration was explored. Experimental tests were carried out using two types of gas gun devices with different calibers. Residual velocity of the projectile was extracted using ls-dyna software for all targets and compared with the experimental data and after validation, the ballistic limit velocity was extracted. Taguchi method was used to design experiments and optimization and ballistic limit velocity, surface density, and strength-to-weight ratio were considered as objective functions. Moreover, the residual velocity of the projectile, damage mechanism of layers, the diameter of the hole at the back layer, central displacement of the back layer, absorbed energy, and changes in the projectile velocity were also investigated. Based on the numerical results, ceramic had the greatest effect on reducing the velocity of the projectile (approximately between 55 and 65%). Strength-to-weight ratio and armor weight were considered as two objective functions in the optimization. The effect of each of the armor materials on the target functions was investigated. According to the results, ceramic had the greatest effect on increasing the strength-to-weight ratio (about 83.88%), and polyurea had the least effect (about 14.09%) on increasing the total weight of the armor.

A novel approach to the design of multi section ultra-wideband Jaumann absorber

A novel ultra-wideband Jaumann absorber design and analytical procedure that greatly enhance the absorption bandwidth and absorptivity level using few dielectric layers are described. The absorptivity bandwidth of the proposed structure is much better than the traditional absorbers. In Jaumann absorbers, determining resistivity of resistive sheets is a complex task and optimization techniques are generally utilized for the solution. The new contribution given in this paper is that the reflected waves from each boundary inside the dielectric layers are eliminated. Therefore, input reflection coefficient is obtained analytically using Binomial expansion without any approximation. Then a binomial matching procedure is applied to obtain the ultra-wideband absorption. Absorption enhancement is achieved by quarter wavelength dielectric slabs, resistive sheets with critical resistivities and a metallic ground plane backed final dielectric layer with high permittivity. Proposed structure is designed for normal illumination. Parallel and perpendicular polarizations are also studied for oblique incidences. Absorptivity and reflectivity variations are presented versus frequency in 0–40 GHz band for the 20 GHz design frequency for several structures. It is shown that choosing three layers backed with a final layer (εrb=20) structure gives absorptivity greater than 90% in a 6.8–33.2 GHz band region with a fractional bandwidth of 132%. CST Microwave Studio and AWR programs are used to prove the correctness of the proposed method. Great consistency is observed and demonstrated in the numerical results section.

Experimental and Numerical Investigation of Ballistic Resistance of Polyurea-Coated Aluminum Plates under Projectile Impacts

The effects of the spraying thickness and the position on the response of aluminum plates under impact loading were studied. The impact tests and numerical simulation were conducted for the penetration process of polyurea-coated 2024 aluminum plates with tungsten sphere impacts. The results indicate the impact resistance performance is similar at slower impact velocity (500–1000 m/s), and the front (or double-side) coating has a smaller advantage. When the impact velocity rises to 1500 m/s, the back coating has a better energy-absorbing performance. The polyurea perform more efficiently with the increase in the impact velocity because the elastomer has large-scale deformation. By comparing the different thicknesses of the back coating, the residual velocity of the fragment has small changes and the impact energy absorption increased with the increase in the coating thickness. The separated phenomenon is serious in front of the bonding face with shear compression failure. In the back polyurea layer, the stripping area is smaller than the front bonding face, and the petaloid cracking is formed with tensile failure.

Bi-layered nanofibers loaded with pomegranate flowers extract as a novel wound dressing: Fabrication, characterization, and in vivo healing promotion

Prolonged and impaired wound healing is a serious complication, and there is an emerging demand for strategies to overcome this problem. In this study, we developed electrospun bilayer nanofibers consisting of a chitosan/PVA active layer loaded with pomegranate flower extract (PFE) and a PCL inactive back layer. Structural, morphological, and physicochemical properties of nanofibers were characterized and wound healing effects of PFE-loaded nanofibers were assessed by in vitro and in vivo experiments, including antibacterial tests, wound closure measurements, and histological and immunohistochemical (IHC) analyses. Nanofibers with optimum formulation showed good hydrophilicity, a mean diameter of 404 nm, and an average roughness of 217 nm. TGA and FTIR studies exhibited typical absorption characteristics of PFE, PCL, and PVA and the compatibility of PFE with the components of bilayer nanofibers. The cumulative amount of PFE released from the monolayer and bilayer fibers after 6 h was measured to be 100% and 64%, respectively, indicating the more sustained release of PFE from bilayer scaffolds. Based on mechanical studies, a more ductile behavior was observed for bilayer fibers than monolayer fibers. The antibacterial assay for PFE-loaded nanofibers exhibited an inhibition zone with a diameter of 19.0 ± 0.5 mm and 15.8 ± 0.3 mm for S. aureus and P. aeruginosa, respectively. PFE-loaded nanofiber exposure contributed to faster wound closure (88% in 14 days) and a significant rise in the expression of α-SMA, CD31, collagen I, and vimentin, indicating a higher tissue regeneration rate for PFE-loaded nanofibers over other treatment groups. In conclusion, bilayer nanofibers containing PFE have a promising potential for wound healing acceleration.