Bacitracin Methylene Disalicylate Research Articles

Xylanase and Direct-Fed Microbials (DFM) Potential for Improvement of Live Performance, Energy Digestibility, and Reduction of Environmental Microbial Load of Broilers.

The challenge of identifying alternatives to subtherapeutic levels of antibiotic growth promoters (AGP) in animal feed has led to increased interest in feed additives such as exogenous enzymes and direct-fed microbials (DFM). Six corn soy-based dietary treatments were designed to investigate the effect of high-efficiency xylanase alone, Bacillus spp. probiotics alone, and their combination vs. a commonly used antibiotic growth promoter (bacitracin methylene disalicylate; BMD) on live performance and environmental Clostridium perfringens load of broiler chickens with eight replicate pens per treatment. Diets were as follows: standard diet (positive control; PC); 130 kcal/kg reduced-energy diet (negative control; NC); NC with xylanase (NC + Xy); NC with probiotics (NC + Pro); NC with xylanase and probiotics mix (NC + XyPro); and NC with BMD (NC + BMD). Data were analyzed as one-way ANOVA. At 35 and 42 days, birds fed with NC + XyPro and NC + BMD were heavier (P < 0.05) than birds fed with NC. Improvement in feed conversion ratio (FCR) (P = 0.0001) was observed from 1 to 42 days by ~3 points in both NC + XyPro and NC + BMD compared to NC. The NC + XyPro reduced lesion scores by 66% compared to PC and NC. Litter C. perfringens cell count was reduced by ~16% with supplementation of XyPro or BMD. It can be concluded that a blend of xylanase (10 XU/g feed) and Bacillus spp. [1 × 105 colony forming units (CFU)/g feed] can be used as an alternative to AGP in low-energy broiler diets.

Effects of cLFchimera peptide on intestinal morphology, integrity, microbiota, and immune cells in broiler chickens challenged with necrotic enteritis

Three hundred and sixty 1-day-old male broiler chicks were randomly allocated to 4 treatments of 6 replicates to evaluate the effects of cLFchimera, a recombinant antimicrobial peptide (AMP), on gut health attributes of broiler chickens under necrotic enteritis (NE) challenge. Treatments were as follows: (T1) unchallenged group fed with corn-soybean meal (CSM) without NE challenge and additives (NC); (T2) group fed with CSM and challenged with NE without any additives (PC); (T3) PC group supplemented with 20 mg cLFchimera/kg diet (AMP); (T4) PC group supplemented with 45 mg antibiotic (bacitracin methylene disalicylate)/kg diet (antibiotic). Birds were sampled for villi morphology, ileal microbiota, and jejunal gene expression of cytokines, tight junctions proteins, and mucin. Results showed that AMP ameliorated NE-related intestinal lesions, reduced mortality, and rehabilitated jejunal villi morphology in NE challenged birds. While the antibiotic non-selectively reduced the count of bacteria, AMP restored microflora balance in the ileum of challenged birds. cLFchimera regulated the expression of cytokines, junctional proteins, and mucin transcripts in the jejunum of NE challenged birds. In conclusion, cLFchimera can be a reliable candidate to substitute growth promoter antibiotics, while more research is required to unveil the exact mode of action of this synthetic peptide.

Evaluation of Bacillus licheniformis-Fermented Feed Additive as an Antibiotic Substitute: Effect on the Growth Performance, Diarrhea Incidence, and Cecal Microbiota in Weaning Piglets.

Simple SummaryMany countries have already restricted the use of antibiotics in preventing diarrhea and improving the growth of weaning piglets. Therefore, exploring alternatives to antibiotics is an urgent unmet need. Bacillus licheniformis-fermented feed additive (BLF)-containing probiotics and antimicrobial substances can diminish the diarrhea incidence of weaning piglets. However, limited information is available in terms of the parallel supplementation of antibiotics and BLF in the diet of weaning piglets. For practical application, this information is important to assess whether BLF can be used as an antibiotic substitute. In this study, the effects of BLF or in combination with bacitracin (antibiotics) on weaning piglets were evaluated. The results showed that the half replacement of bacitracin with BLF leads to positive effects on the alleviation of diarrhea incidence and modification of cecal microbiota in weaning piglets.This study investigated the potential of a Bacillus licheniformis-fermented feed additive (BLF) as an antibiotic substitute in weaning piglets. Ninety-six crossbred piglets were randomly allotted into four treatments with three replicate pens per treatment and eight pigs per pen. Piglets were fed diets as follows: a basal diet as control, a basal diet supplemented with bacitracin (30 mg/kg of bacitracin methylene disalicylate), a basal diet supplemented with BLF (1 g/kg of the Bacillus licheniformis-fermented feed additive), and a basal diet supplemented with bacitracin and BLF (15 mg/kg of bacitracin methylene disalicylate and 0.5 g/kg of the Bacillus licheniformis-fermented feed additive). The results showed that replacing all or half the bacitracin with BLF both reduced the incidence of diarrhea in weaning piglets from day 1 to 14. Principal coordinates analysis and a species abundance heat map showed that distinct clusters were formed between groups. Replacing all the bacitracin with BLF reduced bacterial evenness in the cecal digesta of weaning piglets, while the inhibitory effect on bacterial evenness was reversed in the group treated with bacitracin in combination with BLF. These results indicated that the half replacement of bacitracin with BLF was able to decrease the incidence of diarrhea and modify cecal microbiota composition in weaning piglets, suggesting that a Bacillus licheniformis-fermented feed additive has good potential as a suitable alternative to antibiotics use in the swine industry.

Dietary Mannan-oligosaccharides potentiate the beneficial effects of Bifidobacterium bifidum in broiler chicken.

This study investigated the effects of dietary Bifidobacterium bifidum (BFD) and mannan-oligosaccharide (MOS), as a synbiotic, on the production performance, gut microbiology, serum biochemistry, antioxidant profile and health indices of broiler chicken. Six dietary treatments were T1 (negative control), T2 (positive control-20mg antibiotic BMDkg-1 diet; BMD: bacitracin methylene disalicylate), T3 (0·1% MOS+106 CFU BFD per g feed), T4 (0·1% MOS+107 CFU BFD per g feed), T5 (0·2% MOS+106 CFU BFD per g feed) and T6 (0·2% MOS+107 CFU BFD per g feed). Significantly (P<0·01) better growth performance and efficiency was observed in birds supplemented with 0·2% MOS along with 106 CFU BFD per g of feed compared to BMD and control birds. Supplementation with 0·2% MOS along with either 106 or 107 CFU BFD per g feed reduced (P<0·01) the gut coliform, Escherichia coli, total plate count, and Clostridium perfringens count and increased the Lactobacillus and Bifidobacterium count. Significantly (P<0·01) higher serum and liver antioxidant enzyme pool, serum HDL cholesterol and lower serum glucose, triglyceride, total cholesterol, cardiac risk ratio, atherogenic coefficient and atherogenic index of plasma were observed in birds supplemented with 0·2% MOS along with 106 CFU BFD per g of feed compared to control or BMD supplemented birds. Better production performance, gut microbial composition, serum biochemistry, antioxidant profile and health indices were depicted by broiler chicken supplemented with 0·2% MOS and 106 CFU BFD per g of feed.

Effects of Dietary Supplementation of Essential Oils as an Alternative to Antibiotic Growth Promoter on Performance of Commercial Broilers

An experiment was conducted to assess the effects of thyme and peppermint essential oils (EO) as an alternative to antibiotic growth promoter (AGP) on the performance of broiler chicken. Total 256 day-old Vencobb broiler chicks were randomly distributed into eight experimental groups with four replicates of eight birds each. Experimental groups were fed with control diet without supplementation of AGP and essential oils (T1), BMD (Bacitracin Methylene Disalicylate) antibiotic @ 500 mg/kg diet (T2), thyme essential oil @ 150 mg/kg diet (T3), 200 mg/kg diet (T4), 250 mg/kg diet (T5), peppermint essential oil @ 200 mg/kg diet (T6), 250 mg/kg diet (T7) and 300 mg/kg diet (T8). The supplementation of thyme oil @ 200 mg/kg and peppermint oil @ 300 mg/kg broiler diet significantly (p less than 0.05) improved b.wt. and b.wt gain. The peppermint oil supplementation @ 300 mg/kg was found to be more beneficial when compared among different oil-supplemented groups. The feed conversion ratio was significantly (p less than 0.05) improved with peppermint oil supplementation @ 300 mg/kg diet. A significant (p less than 0.05) reduction in the mean abdominal fat was observed in diet supplemented with higher levels of thyme oil (@ 250 mg/kg diet) and peppermint oil (@ 300 mg/kg diet). Return over feed cost was the highest in broilers assigned diet with AGP; however, it was statistically similar to the basal diet. Considering the net return over fed cost of broilers, use of thyme oil was uneconomical, but supplementation of peppermint essential oil @ 200 and 300 mg/kg diet increased the net return over feed cost and were economical, however less as compared to T1 and T2.

Effect of Dietary Supplementation with Coarse or Extruded Oat Hulls on Growth Performance, Blood Biochemical Parameters, Ceca Microbiota and Short Chain Fatty Acids in Broiler Chickens.

Simple SummaryOat hull (OH) is an excellent source of insoluble fiber, which may improve chickens’ gastrointestinal health by promoting the growth of beneficial bacteria. Extrusion may modify OH fiber fractions resulting in a differential effect on gut microbiota. Thus, the purpose of this study was to determine the effect of dietary supplementation with coarse or extruded OH on growth performance, blood biochemistry and cecal short-chain fatty acids (SCFA) and microbiota of broiler chickens. Broiler chickens were assigned four diets: a corn-wheat-soybean-based diet, an antibiotics diet, 3% coarse OH (COH), and 3% extruded OH (EOH) supplemented diets. Birds fed COH and EOH had reduced BWG, but there was no diet effect on FCR. There was a significant increase in relative gizzard weight among birds that were fed COH. Dietary treatments had no effect on blood biochemical parameters and SCFA concentration. Cecal microbial composition of chickens was mostly comprised of Firmicutes and Tenericutes. Seven bacteria species that were differentially abundant among treatments were identifiedThe purpose of this study was to determine the effect of dietary supplementation with coarse or extruded oat hulls on growth performance, blood biochemistry, cecal microbiota, and short chain fatty acids (SCFA) in broiler chickens. Chickens were randomly allotted to four dietary treatments consisting of a corn-wheat-soybean meal-based diet (Basal), Basal + Bacitracin methylenedisalicylate (BMD), Basal +3% coarse OH (COH), and basal +3% extruded OH (EOH). Feed intake (FI), body weight gain (BWG), and feed conversion ratio (FCR) were recorded weekly. On day 36, eight chickens/treatment were euthanized, blood samples were collected, and organ weights were determined. Cecal digesta samples were collected for the determination of SCFA concentration and microbial DNA sequence. Data were subjected to ANOVA using the mixed procedure of SAS. Alpha diversity was estimated with the Shannon index, and the significance of diversity differences was tested with ANOVA. Birds fed COH and EOH had reduced (p < 0.05) BWG, but there was no effect of treatment on FCR. There was a significant increase (p = 0.0050) in relative gizzard empty weight among birds that were fed COH, compared to the other treatments. Dietary treatments had no effect on blood biochemical parameters and SCFA concentration. Cecal microbial composition of chickens was mostly comprised of Firmicutes and Tenericutes. Seven OTUs that were differentially abundant among treatments were identified. In conclusion, supplementation of broiler chickens’ diets with 3% COH or EOH did not affect the FCR, blood biochemical parameters and SCFA concentration, but modified few cecal microbiota at the species level. Dietary supplementation with COH but not EOH significantly increased the relative gizzard weight.

Composting of chicken litter from commercial broiler farms reduces the abundance of viable enteric bacteria, Firmicutes, and selected antibiotic resistance genes

We examined the ability of composting to remove ARGs and enteric bacteria in litter obtained from broiler chickens fed with a diet supplemented with Bacitracin methylene disalicylate (BDM) (conventional chicken litter), or an antibiotic-free diet (raised without antibiotic (RWA) chicken litter). This was done by evaluating the litter before and after composting for the abundance of ten gene targets associated with antibiotic resistance or horizontal gene transfer, the composition of the bacterial communities, and the abundance of viable enteric bacteria. The abundance of gene targets was determined by qPCR and the microbial community composition of chicken litter determined by 16S rRNA gene amplicon sequencing. Enteric bacteria were enumerated by viable plate count. A majority of the gene targets were more abundant in conventional than in RWA litter. In both litter types, the absolute abundance of all of the target genes decreased after composting except sul1, intI1, incW and erm(F) that remained stable. Composting significantly reduced the abundance of enteric bacteria, including those carrying antibiotic resistance. The major difference in bacterial community composition between conventional and RWA litter was due to members affiliated to the genus Pseudomonas, which were 28% more abundant in conventional than in RWA litter. Composting favoured the presence of thermophilic bacteria, such as those affiliated with the genus Truepera, but decreased the abundance of those bacterial genera associated with cold-adapted species, such as Carnobacterium, Psychrobacter and Oceanisphaera. The present study shows that chicken litter from broilers fed with a diet supplemented with antibiotic has an increased abundance of some ARGs, even after composting. However, we can conclude that fertilization with composted litter represents a reduced risk of transmission of antibiotic resistance genes and enteric bacteria of poultry origin to soil and crops than will fertilization with raw litter.

Sanguinarine modulate gut microbiome and intestinal morphology to enhance growth performance in broilers.

Sanguinarine is a bioactive compound as a quaternary benzophenanthridine alkaloid from plant of the Macleaya cordata, Papaveraceae family. The present study was conducted to investigate the effects of dietary sanguinarine supplementation on growth performance, serum biochemistry parameters, intestinal mucosal morphology and gut microbiome in yellow feathered broilers. Two hundred and seventy 1-d-old female broilers were randomly assigned to 3 treatments ① Basal diet (NG); ② Basal diet containing bacitracin methylene disalicylate (50mg/Kg diet) (ANT); ③ Basal diet containing sanguinarine (0.7 mg/ kg of feed) (SAG). The statistical results showed that dietary sanguinarine supplementation enhanced growth performance and decreased glucose, uric acid as well as urea nitrogen levels of broilers at 28d of age (P<0.05). The 16S rRNA gene sequence analysis revealed that sanguinarine significantly decreased the species from the phyla Bacteroidetes, and increased the species from phyla Firmicutes. Moreover, dietary sanguinarine supplementation improved mucosal morphology to achieve higher ratio of intestinal villus height to crypt depth (P < 0.05), and decreased the concentrations of TNF-α and IL-4 in jejunum mucosal. This study demonstrated that sanguinarine supplementation in the diet of yellow feathered broilers improved intestinal morphology and microbiota community structure to promote growth performance on 1-28d.

Chicken Intestinal Mycobiome: Initial Characterization and Its Response to Bacitracin Methylene Disalicylate.

The gastrointestinal (GI) tract harbors a diverse population of microorganisms. While much work has been focused on the characterization of the bacterial community, very little is known about the fungal community, or mycobiota, in different animal species and chickens in particular. Here, we characterized the biogeography of the mycobiota along the GI tract of day 28 broiler chicks and further examined its possible shift in response to bacitracin methylene disalicylate (BMD), a commonly used in-feed antibiotic, through Illumina sequencing of the internal transcribed spacer 2 (ITS2) region of fungal rRNA genes. Out of 124 samples sequenced, we identified a total of 468 unique fungal features that belong to four phyla and 125 genera in the GI tract. Ascomycota and Basidiomycota represented 90% to 99% of the intestinal mycobiota, with three genera, i.e., Microascus, Trichosporon, and Aspergillus, accounting for over 80% of the total fungal population in most GI segments. Furthermore, these fungal genera were dominated by Scopulariopsis brevicaulis (Scopulariopsis is the anamorph form of Microascus), Trichosporon asahii, and two Aspergillus species. We also revealed that the mycobiota are more diverse in the upper than lower GI tract. The cecal mycobiota transitioned from being S. brevicaulis dominant on day 14 to T. asahii dominant on day 28. Furthermore, 2-week feeding of 55 mg/kg BMD tended to reduce the cecal mycobiota α-diversity. Taken together, we provided a comprehensive biogeographic view and succession pattern of the chicken intestinal mycobiota and its influence by BMD. A better understanding of intestinal mycobiota may lead to the development of novel strategies to improve animal health and productivity.IMPORTANCE The intestinal microbiota is critical to host physiology, metabolism, and health. However, the fungal community has been often overlooked. Recent studies in humans have highlighted the importance of the mycobiota in obesity and disease, making it imperative that we increase our understanding of the fungal community. The significance of this study is that we revealed the spatial and temporal changes of the mycobiota in the GI tract of the chicken, a nonmammalian species. To our surprise, the chicken intestinal mycobiota is dominated by a limited number of fungal species, in contrast to the presence of hundreds of bacterial taxa in the bacteriome. Additionally, the chicken intestinal fungal community is more diverse in the upper than the lower GI tract, while the bacterial community shows an opposite pattern. Collectively, this study lays an important foundation for future work on the chicken intestinal mycobiome and its possible manipulation to enhance animal performance and disease resistance.

Effect of Oat Hulls Incorporated in the Diet or Fed as Free Choice on Growth Performance, Carcass Yield, Gut Morphology and Digesta Short Chain Fatty Acids of Broiler Chickens

The use of high fiber agricultural byproducts in poultry nutrition can help not only to reduce feed cost but also to promote gastrointestinal functionality and growth performance and enhance environmental sustainability. This study was conducted to examine the effect of oat hulls (OH) incorporated in the diet or fed as free choice on growth performance, intestinal morphology, cecal short chain fatty acids (SCFA) production and carcass yield of broiler chickens. Day old broiler chickens were assigned to 4 dietary treatment groups which consisted of a corn–soybean meal–wheat based diet (Basal), Basal + bacitracin methylene disalicylate (BMD), Basal + 3% OH (3% OH) and Basal + free choice OH (FCOH). Each group had six replicate pens of 27 chicks and were raised for 39 d. Feed intake (FI), body weight gain (BWG), and feed conversion ratio (FCR) were determined weekly for each pen. One chicken/pen was slaughtered on d 36 to determine organ weights, jejunal morphology, digesta pH and cecal SCFA and two chickens/pen were slaughtered on d 39 to determine carcass weight and yield. Diet had no effect on FI, except on d 28, when the FCOH group had higher FI than the 3% OH groups. During the starter phase, chickens fed 3% OH had the highest BWG and lowest FCR, which were significantly different (p &lt; 0.05) from those fed FCOH. Chickens fed 3% OH had the highest slaughter, hot carcass, and cold carcass weights, which were significantly different (p &lt; 0.05) from those fed FCOH. Diet had no effect on jejunal villus height and crypt depth, ileal and cecal digesta pH, and cecal SCFA. Chickens fed FCOH had a larger (p &lt; 0.0001) gizzard weight and showed a tendency to have a larger (p = 0.09) ceca weight than other treatment groups. In conclusion, the inclusion of 3% OH in an antibiotic-free diet has the potential to enhance growth performance, while free choice OH increases the gizzard weight of broiler chickens.

Comparative Effects of Spray-Dried Plasma and Bacitracin Methylene Disalicylate on Intestinal Development in Broiler Chicks

Comparative Effects of Spray-Dried Plasma and Bacitracin Methylene Disalicylate on Intestinal Development in Broiler Chicks

Efficacy of Trace Mineral-Amino Acid Complexes in a Necrotic Enteritis Challenge Model.

Necrotic enteritis (NE) is a common and costly disease of poultry caused by virulent toxigenic strains of Clostridium perfringens. Although the importance of trace minerals for intestinal integrity and health is well documented, there is little information on their role in ameliorating the effects of NE. The two studies reported here examined the effects of replacing a portion of the dietary zinc (Zn), copper (Cu), and manganese (Mn) supplied as sulfates in the control diets with metal-amino acid-complexed minerals in a NE-challenge model consisting of coccidiosis and Clostridium perfringens. In a 28-day battery study, the treatments were the following: (1) no additional Zn or Mn, unchallenged (negative control); (2) no added Zn or Mn, challenged (positive control); (3) added ZnSO4 and MnSO4 at 100 ppm each, challenged; (4) additional ZnSO4 at 60 ppm, Availa-Zn at 40 ppm (Low), and MnSO4 at 100 ppm, challenged; (5) added ZnSO4 at 60 ppm, Availa-Zn at 60 ppm (high), and MnSO4 at 100 ppm, challenged; and (6) added ZnSO4 at 60 ppm, Availa-Zn at 40 ppm, MnSO4 at 60 ppm, and Availa-Mn at 40 ppm, challenged. None of the treatments ameliorated gross lesion scores, but all reduced NE-associated mortality compared with the positive control. At 28 days, the group supplemented with Availa-Zn at 40 ppm (low) had a lower body weight than challenged groups supplemented with Zn and the negative control. In a floor pen study, the five treatment groups were the following: (1) Zn, Mn, and Cu from sulfate sources at 100, 100, and 20 ppm respectively; (2) Zn, Mn, and Cu from sulfate sources at 40, 100, and 20 ppm, respectively, plus Zn from Availa-Zn at 60 ppm; (3) Zn and Mn from sulfate sources at 40 and 100 ppm, respectively, plus Zn from Availa-Zn at 60 ppm and Cu from Availa-Cu at 10 ppm; (4) Zn, Mn, and Cu from sulfate sources at 60, 60, and 20 ppm, respectively, plus Zn and Mn from Availa-Zn/Mn at 40 and 40 ppm, respectively; and (5) bacitracin methylene disalicylate at 55 g/metric ton with Zn, Mn, and Cu from sulfate sources at 100, 100, and 20 ppm, respectively (Zoetis, Inc., Kalamazoo, MI). None of the treatments reduced lesion scores. The Availa-Zn and Availa-Zn/Mn had lower mortality than the sulfate-supplemented feed, whereas Availa-Zn/Cu and bacitracin methylene disalicylate were intermediate and did not differ from the other groups. Considering both trials together, and by using NE mortality as the discriminating factor, we found that adding Zn and Mn exceeding National Research Council requirements reduced NE-associated mortality, and in the floor pen study, complexed Zn and complexed Zn plus Mn appeared to be superior to sulfates.

Lippia gracilis Schauer essential oil as a growth promoter for Japanese quail

The use of antibiotics as performance enhancers in animal feeding is declining, so Lippia gracilis Schauer essential oil (LGSEO) could be used as a potential substitute for the conventionally used growth promoters. The LGSEO contains components such as carvacrol and thymol, which kill and/or control pathogenic bacteria, increase population of beneficial organisms, act against oxidative processes and onto nutrient digestibility and absorption. The aim of this study was to investigate the action and the effects of LGSEO as a growth promoter in the diet of Japanese quail by examining their productive performance, intestinal microbiology, blood biochemical parameters, hepatic thiobarbituric acid reactive substances (TBARS) content and intestinal gene expression. A total of 252 two-day-old quail (Coturnix coturnix japonica) were assigned to 3 treatments in 7 replicates, using 12 birds per experimental unit. The treatments consisted of a basal diet, basal diet + LGSEO at 400 mg/kg of diet and basal diet + chemical antimicrobial (bacitracin methylene disalicylate) at 500 mg/kg of diet. The experimental period was 34 days. The highest feed intake (P < 0.01) was found in the group receiving the conventional antimicrobial, whereas the best feed conversion (P < 0.01) was shown by the animals receiving LGSEO. Escherichia coli growth was restricted in the quail receiving the growth promoters. Salmonella spp. growth was controlled by the treatment containing the conventional antimicrobial. There was no difference between the treatments (P > 0.05) for the concentration of aspartate aminotransferase and alanine aminotransferase enzymes in the blood or hepatic TBARS content. Birds receiving negative-control treatment exhibited a higher expression of sodium-glucose cotransporter (SGLT1), while those receiving the treatment with essential oil showed lower catalase (CAT) and glutathione peroxidase (GPX7) expressions compared to the conventional antimicrobial and control groups, respectively. Lippia gracilis Schauer essential oil is a powerful performance enhancer for Japanese quail by virtue of its abilities to improve their intestinal environment, balance the microbial population and reduce energy expenditure for oxidative processes.

Suplementation of Antibiotic Growth Promoters versus Supplementation of Functional Oils in Broiler Breeders: Performance and Offspring Effects

ABSTRACT The effects of either functional oil or antibiotic growth promoter supplementation on broiler breeders and their offspring were assessed in 2 experiments. In Experiment I, 16,400 broiler breeders (22 weeks old) were distributed into 2 experimental groups, one supplemented with 1,500 ppm of a commercial blend of functional oils (active ingredients: cashew nutshell liquid and castor oil) and the other with 500 ppm of bacitracin methylene disalicylate (BMD). Laying rates, fertility, embryonic mortality, and egg characteristics were measured from weeks 25 to 46. In Experiment II, 720 one day old male chicks, born from 42 week old breeders, from each one of the two treatments in Experiment I, were supplemented with either 10 ppm of enramycin or 1,500 ppm of the same functional oils as in Experiment I to study whether there were any maternal diet carry-over effects. Functional oils decreased embryonic mortality from 11.01% to 9.64% (P < 0.03) when compared to BMD. However, a functional oil link increase in egg weight did not result in either heavier chicks or statistically significant better offspring performance. Nonetheless, a possible benefit of the functional oil supplementation on the offspring weight at 42 d cannot be excluded. The type of additive supplemented during Experiment II did not affect any performance parameters. In conclusion, functional oils could successfully replace antibiotic growth promoters in broiler breeders as embryonic mortality decreased. Finally, when functional oils were supplemented to the offspring, the performance was similar to that of a commonly used antibiotic growth promoter.

The effects of dietary Bacillus subtilis supplementation, as an alternative to antibiotics, on growth performance, intestinal immunity, and epithelial barrier integrity in broiler chickens infected with Eimeria maxima

The objective of this study was to investigate the effects of dietary Bacillus subtilis supplementation on growth performance, jejunal lesion scores, oocyst shedding, and cytokine and tight junction protein expression in broiler chickens infected with Eimeria maxima. A total of 196 male day-old Ross 708 broilers were given a nonexperimental diet until 14 D of age. Then, all chickens were randomly assigned to one of seven dietary treatments: 2 basal diets (CON and NC); CON + virginiamycin (AB1); CON + bacitracin methylene disalicylate (BMD; AB2); CON + B. subtilis 1781 (PB1); CON + B. subtilis 747 (PB2); or CON + B. subtilis 1781 + 747 (PB3). At day 21, all chickens except those in the CON group were orally inoculated with E. maxima oocysts. At 7 D after E. maxima infection, the body weight gains of chickens fed PB2 and PB3 increased (P = 0.032) as much as those in chickens fed AB2. The body weight gain and feed efficiency of chickens fed PB2 were significantly increased (P < 0.001), and PB2 chickens showed (P = 0.005) the lowest lesion scores after E. maxima infection. Chickens fed PB2 showed (P < 0.05) lower mRNA expression of IL-1β in infected chicken groups. Chickens in the AB1, AB2, PB1, PB2, and PB3 groups showed (P < 0.05) greater mRNA expression of junctional adhesion molecule 2 in jejunal tissue, whereas occludin expression increased (P < 0.05) in the jejunal tissue of chickens fed AB2 or PB2. Dietary B. subtilis supplementation significantly improved the growth performance of young chickens to a level comparable with that induced by virginiamycin or BMD without E. maxima infection. After infection with E. maxima, dietary virginiamycin and BMD significantly enhanced the epithelial barrier integrity, and the dietary B. subtilis 747 showed significantly enhanced growth performance, intestinal immunity, and epithelial barrier integrity. Together our results indicated that certain strains of B. subtilis provide beneficial effects on the growth of young broiler chickens and have the potential to replace antibiotic growth promoters.

Bacillus licheniformis-Fermented Products Reduce Diarrhea Incidence and Alter the Fecal Microbiota Community in Weaning Piglets.

Simple SummaryAntibiotics have been commonly used worldwide as growth promoters and for prophylactic treatment of diarrhea in weaning piglets. However, The European Union has banned the use of antibiotic growth promoters in animal production. Therefore, finding alternative solutions for preventing diarrhea in weaning piglets is urgent. Modulation of gut microbiota composition by probiotics has a beneficial effect on animal health. In this study, we assessed the effects of Bacillus licheniformis-fermented products on diarrhea incidence and the fecal microbiome composition in weaning piglets. Results showed that B. licheniformis-fermented products could improve diarrhea incidence and the fecal microbiota community in weaning piglets. These findings indicate that B. licheniformis-fermented products have the potential for development as feed additives and use as possible substitutes for antibiotics to prevent postweaning diarrhea in the pig industry.Prophylactic use of antibiotics in-feed has been effective in decreasing the incidence of diarrhea in weaning piglets. However, the overuse of antibiotics as prophylactic or therapeutic agents in animal feed leads to the evolution of drug-resistant bacteria and antibiotic residues in pigs. This study investigated the effects of Bacillus licheniformis-fermented products on diarrhea incidence and the fecal microbial community in weaning piglets. A total of 120 crossbred piglets with an average initial body weight of 9.87 ± 1.43 kg were randomly allotted to four dietary treatments consisting of three replicate stalls with 10 piglets in each. The dietary treatments comprised a basal diet as control, control plus 1 g/kg or 4.5 g/kg of B. licheniformis-fermented products, and control plus 30 mg/kg antibiotics (bacitracin methylene disalicylate). Results showed that 4.5 g/kg of B. licheniformis-fermented product supplementation significantly reduced diarrhea incidence in weaning piglets. Principal coordinate analysis and a heatmap of species abundance indicated distinct clusters between the groups treated with antibiotics and B. licheniformis-fermented products. The bacterial richness and evenness in the feces decreased in weaning piglets fed 1 g/kg of B. licheniformis-fermented products and antibiotics. The abundance of the genera [Ruminococcus] gauvreauii group, Ruminococcaceae UCG-005, and Ruminococcaceae UCG-008 in the feces decreased in weaning piglets fed B. licheniformis-fermented products or antibiotics. The average abundance of the genus Prevotella 9 in the feces was positively correlated with the concentration of B. licheniformis-fermented products and negatively correlated with the diarrhea incidence in weaning piglets. Furthermore, the average abundance of the genus Prevotella 9 in the feces was positively correlated with the growth performance of weaning piglets. These results demonstrate that B. licheniformis-fermented products can improve diarrhea incidence and fecal microflora composition in weaning piglets.

Effect of the Addition of Humic Substances as Growth Promoter in Broiler Chickens Under Two Feeding Regimens.

Simple SummaryThe rapid spread of antimicrobial-resistant genes in bacterial communities is a threat to human, animal, and environmental health that continues to progress inflexibly. Humic substances (HS) are promising complex molecules as an alternative to reduce the use of growth promoter antibiotics (GPA) in animal feeds. Improvements in productivity, intestinal health, immune response, and antioxidant status have been reported in broilers supplemented with HS. In the present study, broilers fed with an extract of HS (EHS) from a worm compost had similar carcass yield and excretion of coccidian oocysts but increased Clostridium perfringens and lactic acid bacteria (LAB) compared to broilers fed diets added with GPA. Broilers subjected to feed restriction had reduced growth performance and meat quality. These results confirm the growth-promoting effect of ESH, which could be explained by higher abundance of the beneficial LAB and by reducing the multiplication of harmful parasites in the gut of broilers.Humic substances (HS) from different sources have been evaluated to replace or reduce the use of growth promoter antibiotics (GPA) in the feeds of broiler chickens. The objective was to evaluate the growth performance, tibia measurements, nutrient balance, meat quality, and microbiological status of broiler fed with an HS extract (EHS) under ad libitum (ADLIB) or feed restriction (REST). Individually caged broilers (n = 180, 14–35 day of age) were assigned to a factorial arrangement of three dietary treatments: (1) positive control with bacitracin methylene disalicylate (BMD) and salinomycin; (2) negative control without BMD nor salinomycin, and (3) same as negative control with 0.25% EHS, and two feeding regimens 1) ADLIB or REST for 24 h on d 1, 7, and 14. Results were subjected to ANOVA. Positive control and EHS-fed broilers showed higher carcass yield (p < 0.05) and lower oocyst excretion (p < 0.01) compared to negative control birds. Lactic acid bacteria (LAB) and Clostridium perfringens (C. perfringens) were higher in negative control and EHS-broilers compared to positive control (p < 0.01). In conclusion, higher carcass yield, lower C. perfringens and oocyst excretion were found in positive control and higher carcass yield, higher LAB and lower oocyst excretion were found in EHS-fed broilers. Broilers subjected to REST had reduced growth performance and meat quality. In conclusion, EHS could be used to increase the carcass yield and beneficial LAB in broilers.

PSIX-35 Alternatives to antibiotic supplementation on broiler performance under highland conditions

Abstract There is limited information on performance and gut microbiota balance response to alternatives to antibiotics growth promoters on broiler at high altitude and level of health challenge. Therefore, Two experiments were conducted to evaluate the effects of alternatives to antibiotics supplementation on broiler performance and health at 2500 m.a.s.l. In, Exp. 1, a total of 1080 day-old Cobb-500 male broilers were used in a completely randomized block design. Eight experimental diets containing 1) Negative Control (NC), 2) Bacitracin methylene Disalicylate at 55 mg/kg (BMD), 3) Direct fed microbial at 750 mg/kg (DFM) 2x1011 CFU/kg of lactic acid bacteria, 4) a blend of essential oils (150 mg/kg; EO), 5) combination of permeabilizers, organic acids and phytochemicals (0.1%, CN), 6) DFM+EO, 7) DFM+CN, and 8) EO +CN. Performance was evaluated through the feeding period of 42 d with a new litter. There were no significant differences in performance nor mortality rate during the whole feeding period. In, Exp. 2, 1232 one-day-old Cobb-500 male chicks were randomly divided into 56 experimental units and exposed to similar treatments as described on Exp. 1. Performance variables, and cecal-microbiota population were evaluated during 42-d with a re-used litter. Overall, daily gain was increased for BMD, DFM, and EO+CN compared to NC (P &amp;lt; 0.05). Feed intake was not altered by dietary treatment (P = 0.892). Feed conversion rate was improved by BMD and EO compared to NC (P &amp;lt; 0.05). Total coliform, and E. coli population was increased by DFM and CN compared to NC (P &amp;lt; 0.005). Total Enterobacteriacea was not altered by dietary treatment with exception of EO, that increased its population compared to remaining treatments (P &amp;lt; 0.001). Data form this experiments suggest that positive effect of organic feed additive supplementation, especially EO, depend on bird health challenge at high altitudes.

Effect of dietary supplementation of prebiotic on growth performance, immune response and intestinal microbial load in broiler chickens

The experiment was conducted to study the effect of using prebiotic (mannan oligosachharide -MOS) in place of antibiotic growth promoter (AGP) on performance, immune response and intestinal microbial load in broiler chickens. Day-old broiler chicks (192) were randomly distributed into 20 groups each of 8 chicks (4 treatments × 6 replicates). Four experimental diets T1, T2, T3 and T4 were formulated to contain no additive, bacitracin methylene di-salicylate (BMD) at 20 mg/kg diet, MOS at 0.1 and 0.2%, respectively. Body weight gain (g) was increased by the feeding of diets containing 0.2% levels of MOS, but feed intake (g), feed conversion ratio (FCR) and mortality (%) did not differ significantly. Antibody (28 d), titres were significantly higher after feeding 0.1 or 0.2% MOS and antibiotic (T2) supplemented group. During d 35, the response to intra-dermally injected phyto-hemagglutinin, an index of the in vivo cell-mediated immune response, was increased in the 0.2% MOS supplemented group. Significant reduction was observed in coliforms and total plate count in cecal (28 and 42 d) and excreta (42 d) in MOS (0.1 or 0.2%) or antibiotic (T2) supplemented groups. Lactobacillus count significantly increased in cecal (28 and 42 d) and excreta (42 d) in MOS @0.1 or 0.2% supplemented groups. Thus, it can be concluded that, 0.2% MOS with basal diet has a beneficial effect for growth performance, immune response and gut health status in broiler chickens, and MOS could be a good alternative to antibiotic growth promoter.

Effects of Direct Fed-Microorganisms and Enzyme Blend Co-Administration on Growth Performance in Broilers Fed Diets With or Without Antibiotics

SUMMARY Direct-Fed Microorganisms (DFM) and exogenous enzymes have been demonstrated to improve growth performance in poultry and are potentially important components of management programs for antibiotic-free (ABF) poultry production. In this study, we evaluated the effect of a feed additive composed of a DFM product containing spores of 3 Bacillus amyloliquefaciens strains and a multi-enzyme complex of xylanase, amylase, and protease on the performance of broilers with or without an antibiotic growth promoter (AGP), either bacitracin methylene disalicylate (BMD) or virginiamycin. Growth performance of broilers fed one of 6 treatments arranged in a 3 (AGP) × 2 (additive) factorial was evaluated over a 42 d production period. Overall, administration of the feed additive improved body weight, body weight gain, and feed conversion ratio. Administration of either antibiotic significantly reduced cumulative FCR when compared to the control, while BMD administration reduced foot pad lesions as compared to the control. Our results suggest the co-administration of DFM and enzymes may be an important component of ABF poultry production programs and the benefits of their use are not dependent on the presence or absence of AGP.