Abstract
Sanguinarine is a bioactive compound as a quaternary benzophenanthridine alkaloid from plant of the Macleaya cordata, Papaveraceae family. The present study was conducted to investigate the effects of dietary sanguinarine supplementation on growth performance, serum biochemistry parameters, intestinal mucosal morphology and gut microbiome in yellow feathered broilers. Two hundred and seventy 1-d-old female broilers were randomly assigned to 3 treatments ① Basal diet (NG); ② Basal diet containing bacitracin methylene disalicylate (50mg/Kg diet) (ANT); ③ Basal diet containing sanguinarine (0.7 mg/ kg of feed) (SAG). The statistical results showed that dietary sanguinarine supplementation enhanced growth performance and decreased glucose, uric acid as well as urea nitrogen levels of broilers at 28d of age (P<0.05). The 16S rRNA gene sequence analysis revealed that sanguinarine significantly decreased the species from the phyla Bacteroidetes, and increased the species from phyla Firmicutes. Moreover, dietary sanguinarine supplementation improved mucosal morphology to achieve higher ratio of intestinal villus height to crypt depth (P < 0.05), and decreased the concentrations of TNF-α and IL-4 in jejunum mucosal. This study demonstrated that sanguinarine supplementation in the diet of yellow feathered broilers improved intestinal morphology and microbiota community structure to promote growth performance on 1-28d.
Highlights
Sanguinarine (C20H14NO4) is a quaternary benzophenanthridine alkaloid from phenylalanine in plants of the Macleaya cordata, Papaveraceae family[1]
Basal diet (NG): basal diet; Basal diet containing Bacitracin methylene disalicylate (BMD) (ANT): basal diet group with 50mg/Kg BMD; Basal diet containing sanguinarine (SAG): sanguinarine supplemented with 0.7 mg/kg of diet; BW, body weight; ADG, average daily body weight gain; ADFI, average daily feed intake; Feed/ gain ratio (FCR), feed/gain ratio. a,b within a row, values with different superscripts differ significantly (P < 0.05)
The results showed that the abundance levels of 14 Operational taxonomic unit (OTU) were markedly changed by sanguinarine and BMD supplementation compared with NG group, while abundance levels of 25 OTUs were markedly changed by age (28d vs 56d)
Summary
Sanguinarine (C20H14NO4) is a quaternary benzophenanthridine alkaloid from phenylalanine in plants of the Macleaya cordata, Papaveraceae family[1]. Macleaya cordata was recognized by the European Food Safety Authority as a feed additive for animal production[2]. Sangrovit is a commercial product extracted from Macleaya cordata which is composed of mainly sanguinarine and chelerythrine, and it is standardized to 1.5% sanguinarine[3]. Previous studies reported that sanguinarine displayed a wide range of pharmacological activities, such as antifungal, anti-inflammatory, antimicrobial, analgesic and anti-cancer properties[4,5,6].
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