Ayurvedic Drug Research Articles

TOXICITY DUE TO CHEMOTHERAPY & RADIOTHERAPY & ITS AYURVEDIC APPROACHES

Chemotherapy and radiotherapy are the treatment modalities for cancer that are extensively used in current times due to their effectiveness against many cancers. These therapies can cause severe toxicities, including loss of appetite, diarrhea, anorexia, constipation, vomiting, burning sensations, weakness, alopecia, and fever. Reduction in hemoglobin, white blood cells, and platelets is among other adverse effects of chemotherapy. In this context, Ayurveda can be an evidence-based proven approach to prevent and resist complications of cancer therapies. Several Ayurvedic drugs such as Brahmi, Brihamarasayan, Guduchi, Yasthimadhu, and Ashwagandha can help alleviate the side effects caused by chemotherapy and radiotherapy. These drugs also offer therapeutic benefits against cancer. Ayurvedic medicines reduce the burning sensation induced by chemotherapy, improve digestion, restore blood counts, remove toxins, and induce mental calmness. In this way, Ayurveda plays a significant role in preventing and managing complications of chemotherapy and radiotherapy. There is a need to explore such approaches that counteract the side effects of chemotherapy and radiotherapy. Considering this fact, the present article explores the role of Ayurvedic modalities in preventing and managing chemotherapy and radiotherapy complications.

Web Application for Recommendation of Ayurvedic Drugs and Medicine using ML

This project aims to develop a comprehensive Ayurvedic drug recommendation website that seamlessly integrates traditional Ayurvedic principles with modern technology. The platform will cater to both students seeking educational resources and practitioners offering personalized healthcare guidance. The website's key features include drug prediction algorithms, herbal drug recommendations based on individualized assessments on symptoms, and a user-friendly interface. The initial phase involves meticulous planning, scoping, and research to define project objectives, target audiences, and select an appropriate technology stack. Subsequent weeks focus on design and prototyping, incorporating user feedback to ensure an intuitive and engaging interface. The front-end development phase emphasizes responsiveness and usability, while the back-end development integrates sophisticated algorithms for drug prediction and drug recommendation. User authentication and authorization mechanisms will be implemented to cater to both students and practitioners, ensuring a secure and personalized experience. The website's robustness and reliability will be validated through comprehensive testing, addressing any identified issues to guarantee a seamless user experience. The refinement and optimization phase involve continuous feedback collection, iterative improvements, and performance optimization. Beta testing will be conducted to gather user insights, refining the platform further. The deployment and launch process will involve hosting the website on a reliable platform, setting up monitoring tools, and officially releasing the Ayurvedic drug prediction and drug recommendation website to the public. This project combines the rich heritage of Ayurveda with cutting-edge technology, offering a valuable resource for education and healthcare in the Ayurvedic domain. The website aims to empower both students and practitioners with accurate drug predictions and personalized drug recommendations based on symptoms, fostering a holistic approach to healthcare that aligns with traditional Ayurvedic principle.

Industrial manufacturing method and characterization of Ayurvedic marine drugs: mother pearl, cowry, coral and pearl

Introduction: Ayurvedic marine drugs derived from mollusc shells and coral are regularly used by Ayurvedic physicians to treat several disease conditions like acid peptic disease, irritable bowel syndrome, osteoporosis, etc. However, standard operating procedures for manufacturing these drugs and their complete characterization have not been published in the Ayurvedic Formulary and Ayurvedic Pharmacopeia of India to date. Methods: Present study describes the traditional manufacturing process and thorough characterization using classical and advanced analytical tools. The raw materials characters, in-process parameters, and finished product specifications have been elaborated to develop monographs. Especially, the identity and purity of raw coral and pearl were checked by Raman Spectroscopy and Energy Dispersive X-ray Fluorescence analysis. Results: In the finished product analysis, the X-Ray Diffraction study revealed that incineration after trituration with Aloe barbadensis leaf pulp or rose water converted the aragonite phase of calcium carbonate into calcite phase in mother pearl, cowry, and pearl while the calcite form of raw coral was retained. The prominent bands around 1390, 870, and 712 cm−1 detected by Fourier Transform-Infrared Spectroscopy and mass loss between 39–44% (w/w) revealed by thermogravimetric analysis confirmed the carbonate form of these calcium-based drugs. The finished products were very fine grayish-white powders constituted by irregularly shaped nano-micro particulate calcium carbonate exhibiting particle size between 600 nm (D10 value) to 1.2 µm (D90 value). Conclusion: The quality control and assurance achieved in this study may be further utilized by the pharmaceutical industries to manufacture quality marine drugs and conduct efficacy studies.

Evaluation of Comparative Efficacy of Levothyroxine Versus Kshar Basti and Kanchanar Guggul in the Treatment of Hypothyroidism: Protocol for a Randomized Controlled Trial.

The thyroid gland is an endocrine gland that has an impact on the body's general metabolism. Thus, the secretions of the thyroid gland can modify the overall metabolism of the entire body. The prevalence of hypothyroidism is increasing quickly, with rates of 2%-5% in affluent countries and 11% in India. Individuals diagnosed with hypothyroidism need to take medication for the rest of their lives, resulting in significant stress. Therefore, conducting a study in this area is imperative. This study aims to assess the effectiveness of the therapeutic enema (Kshar Basti) and oral Kanchanar Guggul in the treatment of hypothyroidism. The trial group (n=45) will receive a therapeutic enema (Kshar Basti) followed by oral Ayurvedic drugs for 180 days. The control group (n=45) will be given levothyroxine tablets at a dosage of 1.6 µg/kg/day for the same duration. The objective is to examine the alterations in thyroid stimulating hormone (TSH) levels before and after the treatment. Any deviation of the serum TSH by more than 20% from the initial values, while keeping triiodothyronine (T3), and thyroxine (T4) levels within the normal range, will be deemed statistically significant. Consequently, we anticipate a statistically significant variation in serum TSH levels between the therapeutic enema and Kanchanar Guggul treatments. Presently, the drug preparation operations are in progress. We expect to start enrolling patients in June 2024, do data analysis in December 2025, and acquire results by early 2026, marking the end of this trial. This study will evaluate the efficacy of the therapeutic enema, specifically Kshar Basti, in treating hypothyroidism. Furthermore, more research can determine the efficacy of a therapeutic enema (Kshar Basti) in treating overt hypothyroidism and hypothyroidism during pregnancy. Clinical Trials Registry India CTRI/2023/05/052389; https://ctri.nic.in/Clinicaltrials/pmaindet2.php?EncHid=Nzk1NjY=&Enc=&userName=052389. PRR1-10.2196/57287.

Phytochemical comparison of selected Sida species using spectrophotometric and multiple chromatographic analyses

BackgroundBala is an important Ayurvedic drug used for the treatment of many diseases. Sida cordifolia (L.), Malvaceae family, is the genuine source drug of Bala as per Ayurvedic Pharmacopeia of India. The other species of Sida like Sida acuta, Sida rhombifolia, Sida alnifolia and Sida cordata are also used as adulterants or substitutes of Bala. The objective of the present study is to identify a scientifically validated substitute for Bala (Sida cordifolia) by detailed phytochemical evaluation of its allied species.ResultsPreliminary analysis showed that all the selected species contain similar class of compounds like alkaloids, phenolics, flavonoids, etc. Quantitative estimation of major class of compounds such as total alkaloids, total phenolics and total flavonoids was done by spectrophotometric methods. Polyphenolic contents of selected species are almost comparable. In the case of alkaloids, variations have been observed among the species. Chemical profiles of selected species were compared by HPTLC and HPLC analysis. Major chemical constituents are found to be common for the selected species. HPLC profiles also showed similarity in their peak pattern.ConclusionBased on the phytochemical studies, the species like S. alnifolia, S. acuta and S. rhombifolia contain almost similar types of phytochemicals. However, the chemical constituents of Sida cordata are found to be different from other allied species. Further studies including pharmacological evaluation are required to ensure the therapeutic properties of allied species to confirm the substitute.

Acute and subacute oral toxicity evaluation of Ayurvedic formulation Tapyadi loha in rats

Ayurveda is one of the oldest systems of traditional medicine that provides treatments for a wide range of acute and chronic health problems. It is a common myth amongst people that Ayurvedic drugs have no side effects, whereas the fact is that these drugs can cause adverse effects. Despite their wide use, the safety data of many Ayurvedic formulations are still unavailable. Tapyadi loha is an Ayurvedic formulation traditionally claimed for iron deficiency anemia in pregnant and non-pregnant patients. However, no scientific study has been conducted to evaluate its oral toxicity. Hence, the present study evaluated the acute and subacute oral toxicity of the Tapyadi loha according to the OECD test guidelines 425 and 407, respectively. Tapyadi loha did not cause mortality nor any signs of toxicity when given once orally at a dose of 2000 mg/kg. Subacute toxicity study showed no mortality as well as no behavioral, hematological, biochemical and histopathological abnormalities in rats treated with Tapyadi loha formulation at 250, 500 and 1000 mg/kg for 28 days. It is concluded that the Tapyadi loha is safe at a single dose of 2000 mg/kg and 28 days repeated dose of 1000 mg/kg by oral route in rats.

Comparative stagewise mapping of trace elements using ICP-OES in five Ayurvedic Marine Drugs highlights their posologic and clinical implications

Rasashastra, an Ayurvedic pharmaceutical branch, scientifically converts natural resources into palatable therapeutic dosage forms. Ayurvedic Marine Drugs (AMD) are mainly calcium carbonate/ calcite, but in Ayurvedic treatment modality they are uniquely used for several ailments. Moreover, their detailed microchemical compositions have seldom been reported. In this study, five raw materials, conch, oyster shell, cowry, coral, and pearl, their transitions during the manufacturing process, and the final drugs were analyzed for identification and quantitation of minor elements using a validated inductively coupled plasma optical emission spectrophotometry method. All the five raw materials contained traces of Al, Ag, B, Ba, Cu, Co, Cr, Fe, Hg, Li, K, Mg, Mn, Na, Ni, Pb, S, Sr, Tl, and Zn. Interestingly, B, Co, Cr, Li, Mn, and Ni were absent in the finished products, whereas Pb and Hg exhibited a considerable decrement during the drug formation. The first three components of principal component analysis together explained 72.09% of variance. Raw AMD indicated positive factor scores, whereas their respective purified and incinerated forms exhibited negative scores. Cluster analysis derived a dendrogram typically forming incinerated AMD group but not for the raw forms. Coral with Mg as highest content was a remarkable outlier in both these analyses as compared to Na in others. In discriminant analysis of the presence/absence of elements and periodic table-based classifications, coral and pearl showed higher variation indicating as discriminants when equated with others. Periodic table-based classification was a better model for discrimination. Correlation analysis in both classifications revealed a strong correlation of conch with oyster shells and vice versa, cowry and pearl with oyster shell and a weak correlation of coral with pearl. Coral was a good predictor followed by conch, pearl, oyster shell, and cowry, sequentially, and can be considered a moderately sensitive but highly specific model. Thus, ICP-OES technique is highly precise and accurate for assessing the micro-composition of raw, in-process, and finished products to ensure their transitions, quality, and genuineness. Such characterized traditional medicines should be further investigated in depth for their mechanistic aspects.Graphical abstract

AYURVEDIC MANAGEMENT OF RHEUMATOID ARTHRITIS WSR TO AMAVATA- A CASE REPORT

Introduction: The Ayurveda described Mandagni as the root cause of all the diseases. The Acharyas mentioned Amavata as one of the common disorders caused by the impairment of Agni, formation of Ama and vitiation of Vata Dosha. The Ama is carried by the vitiated Vata and deposited in Shleshmasthanas producing symptoms like Angmarda, Aruchi, Alasya, Sandhiruk and Sandhishoth. On the basis of sign and symptoms Amavata can be equated to Rheumatoid Arthritis in modern era. Rheumatoid arthritis is a systemic autoimmune disease characterised by inflammatory arthritis and extra articular involvement. The onset of disease, beginning with prodrome of fatigue, weakness, joint stiffness, vague arthralgias followed by pain and swelling of joints. Aim: To evaluate the efficacy of Oral Ayurvedic drugs in management of Amavata. Method: In the present case, a female patient aged 36 years presented in OPD Of Asthi-SandhirogaVibhaga of RGGPG Ayurvedic College Paprola Distt. Kangra (H.P.) with the complaints of pain in multiple joints since 2 years. She also complained of generalised weakness and morning stiffness. The investigations were advised and oral Ayurvedic formulations like Vishtindukvati, Amavatari Ras and TrikatuChurna were prescribed to the patient. Result: The Ayurvedic management showed desired results in relieving the symptoms of patient. Discussion: The Ayurvedic drugs are effective in treating Amavata equated to RA and better alternative to NSAIDS, DMARDS and steroids in Rheumatoid arthritis management in todays world.

Study of Nausea and Vomiting can be Symptoms of Many Different Conditions

Abstract: Before the twentieth century, drugs used for the treatment of diseases were obtained from natural sources like plants, animals, microorganisms, and minerals, and among them, plants were the major source of natural drugs. At present, most of the drugs are obtained from synthetic and biosynthetic sources. The nature was once served as the source of all medicaments and plants, especially the higher plants have been continuing the service since antiquity as important sources of novel compounds useful directly as medicinal agents, as model compounds for synthetic or semi-synthetic structure modifications and optimization, as biochemical and/or pharmacological probes, and as sources of inspiration for generations of synthetic organic medicinal chemists. Plant-derived compounds which have recently under- gone development include the anti-cancer agents, taxol and camptothecin, the Chinese antimalarial drug, artemisinin, and the East Indian Ayurvedic drug, for-skolin. These and many other examples serve to illustrate the continuing value of plant-derived secondary metabolites as viable compounds for modern drug devel- opment (Newman et al. 2003; Newman and Cragg 2007). Natural sources are most primitive and abundant. Drugs obtained from the natural sources include a. Plant, b. Animal, c. Microbial, d. Marine, e. Mineral, and f. Geographical sources. Plant, Animal, Microbial and Marine may be put under common heads—the biological sources.

Argemone Mexicana is a Plant with Anti-Cancer & Anti-Inflammatory Properties. A Review of its Traditional uses & Scientific Evidence

Argemonea Mexicana, generally known as the Mexican prickly poppy of the family Papavaraceae, is a plant that's well known for its medicinal quality. The plant is set up in tropical regions and is used in Ayurvedic, Unani, Siddha and Homeopathic drug practies, Each part of the plant contains bioactive composites that help in treating a variety of affections similar as' HIV', malaria, ringworm infection, fungal Infections & cancer. The plant has been studied in vivo & in vitro the results have been positive. The Plant also contains composites like berberine, argemonine & protopine, which are known for their restorative action. It's considerably used traditionally for the treatment of multitudinous conditions.Aregmone Mexicana is reported to have antimicrobial exertion, crack mending capacity in rats, larvicidal and chemosterilant exertion, and Nematicidal & allelopathic eventuality. In Mexico Infusion of the upstanding part of the plant is used as hypoglycemic. Chemical disquisition of the plant has revealed the presence of alkaloids, amino acids, phenolics, & adipose acids. The upstanding corridor of the plant contain" Isoquinoline" and" Benzylisoquinoline ” alkaloids. Medicinal plant are extensively used in the treatment of habitual conditions, metabolic conditions, and cancer. Argemone Mexicana L.(A. mexicana), substantially set up in the remains of Marathwada( Maharashtra, India), has been used in the treatment of speech diseases. In this research, cold water, and methanolic extricates were arranged from the Argemone mexicana stem and taken off. These extricates were tried for their antifungal and anticancer exercises. The antifungal action was tried exercising the Agar well dispersion strategy, whereas the anticancer motion against eternalized cell lines was examined by the Erythrosin blue experiment.

A COMPREHENSIVE STUDY OF MURRAYA KOENIGII (LINN.) ON RESTORATION OF CONSCIOUSNESS BY AUGMENTING GLUCOSE UPTAKE IN THE NEURONS: A REVIEW

Fainting, also known as syncope or passing out, is caused by decreased cerebral blood flow leading to loss of consciousness and muscle strength. It is caused by lack of blood supply to the brain leading to decrease blood sugar supply to the neurons. Symptoms like dizziness, sweating, pale complexion, blurred vision, nausea, vomiting or a warm feeling precede the loss of consciousness[1]. For the brain, glucose serves as its main energy source. In order for glucose to be transported from the blood into the brain, it must pass through the endothelial cells that line the blood-brain barrier and enter the neurons and glia. These processes are mediated by facilitative Glucose transporter protein. Kaidarya is an Ayurvedic drug from the ancient samhitas, commonly known as curry leaves. Due to its grahi properties it increases the glucose uptake activity. When glucose uptake activity increases in the brain, it supplies sufficient glucose to the brain cells, which helps in management of fainting or syncope.

Management of Splenomegaly (Pleehodara) with Classical Herbomineral Drugs

With proper knowledge and timely intervention, Ayurvedic herbomineral drugs can reverse diseases without any side effect. Splenomegaly is a rare disorder that affects 2% of Americans in total, according to estimates of prevalence. It is need of time to work more over the traditional medicine like Ayurveda to search new dimension in this field. Some Ayurvedic seers have mentioned Yakritodar in separate chapters and some others has included under Udararoga heading. It was assumed as Kapha-pitta disorders among various herbal and herbomineral drugs they have focused in prescribing Kshara and drugs which can pacify Kapha-pitta. Drugs like Yakritplihari lauha, Pippali churna, Rohitakarista, Arogyavardhini vati are not only effective in reducing the size of spleen but also provide relief in many other systemic symptoms. The total duration of treatment was 3 months. The patient was assessed for improvement in hematological parameters, ultrasound report and signs and symptoms. Hence, presenting this case is evidence to demonstrate the effectiveness of 3 months of Ayurvedic treatment in managing splenomegaly.

A Comparative Clinical Study on the Effect of Tagar (Valleriana Wallichii Dc.) and Aswagandha (Withania Somnifera) in the Management of Preoperative Anxiety

Preoperative anxiety is a clinical condition which requires medical management apart from reassurance. Surgical patients have the high incidence of anxiety. Relief from anxiety is accomplished by pharmacological and non-pharmacological measures, often there is no need of a therapeutic agents for control of preoperative anxiety. Various drugs are used for control of preoperative anxiety in the ward. Ayurveda has an age proved natural ways for the management of disease. Many Acharya have prescribed Rasayana and Aushadha for treatment of mental illness which is safe and effective under optimal doses. Method: Here is a study has been planned for Rogi Sambandhit Purva Karma in which administration of pre–operative medication and other measures are done to relieve the anxiety and other conditions. So, the present study is conducted to evolve an effective Ayurvedic drugs for management of preoperative anxiety. Here in this study Tagar Churna has been taken for study. Various ancient texts and online journals related to the topic have been reviewed. Result: Results were obtained by analysing the data using student’s t-test. Discussion: Discussions were done regarding entire clinical study and the results obtained with relative opinions and arguments.

Extraction of Chemical constituents from Flaxseeds and Fenugreek seeds by different Extraction methods and screening of their activity

The seeds of fenugreek and flaxseeds are ayurvedic drugs with no side effects that are known to control many diseases. When extracted using harmful chemicals such as petroleum ether, hexane, etc., flaxseed oil and fenugreek oil's had an ability to control blood glucose levels. Hence, we intend to extract these oils using green chemistry principles. Accordingly, we plan to test by using four different methods of extracting flaxseed and fenugreek oil, following green chemistry principles, to determine which yields the most effective results. We used mol inspiration web software to calculate some of the QSAR parameters in this study. Then, using an in vitro method known as DNSA, we screened for anti-diabetic activity with the help of UV. We determined the combined and individual anti-diabetic effects of both oils.

An Ayurvedic Approach in Treatment of Chronic Kidney Disease: A Case Series

Background: Akidney damage or glomerular filtration rate (GFR) < 60ml / min/1.73m for 3 months or more irrespective of cause is define as a chronic kidney disease. In many kidney diseases, kidney damage can be ascertained by the presence of albuminuria, defined as albumin – creatinine ratio > 30 mg / gm in two of three spot urine specimens. Severity of kidney diseases is classified into five stages according to level of GFR from G1 to G5.  Methods- Case series of 10 patients were managed with Ayurvedic treatment. Assessment was done on the basis of KFT, CBC, ESR and GFR  Result- Improvement in GFR and Decreased Sr. creatinine and urea level will be stated in full paper.  Discussion and Conclusion- After administration of Ayurvedic drug changes in KFT observed with changes in GFR, which is mention in full paper. ON this we concluded that Ayurvedic treatment is beneficial in treatment of chronic kidney failure than acute kidney failure.

Pharmacognostic evaluation of ayurvedic drug sariva and its commonly known adulterant and substitutes

Pharmacognostic evaluation of ayurvedic drug sariva and its commonly known adulterant and substitutes

EFFECTIVE AYURVEDIC DRUGS FOR HYPERLIPIDEMIA : REVIEW ARTICLE

Hyperlipidemia is a condition in which blood contains an abnormally high amount of lipids i.e. fats. These factors can accumulate and cause blockages in blood vessels. This is why having high cholesterol may increase the risk of damaging organs that don’t receive enough blood from arteries. Life threatnening emergencies like heart attack and brain stroke can arise from untreated chronic hyperlipidemia. Today’s sedentary lifestyle, ready to eat or instant food items, increasing use of technologies which decreases physical activities, all these factors prone for increase in abnormal lipid levels in body. Foods that have cholesterol, saturated fat, and trans fats can raise blood cholesterol level. These include Cheese, Fried and processed foods, Ice cream, Pastries, Red meat etc. According to WHO, the global prevalence of raised total cholesterol among adults was 39% (37% for males and 40% for females) in 2008. According to a study by ICMR-INDIAB, prevalence of dyslipidemia in a large representative sample of four selected regions in India showed that 79% had abnormalities in one of the lipid parameters. Meda dhatu from Ayurveda is an entity which is correlated to lipids. Medoroga, Medodosha, Sthaulya are the synonyms used for diseases with excessive formation and accumulation of Meda dhatu. Two main treatment modalities present in Ayurveda, Shodhan and Shaman, play an efficient role in treating this disease. So, there is a challenge of treating the disease without interference in the daily routine of an individual. This paper specifically throws light on meeting aforesaid needs with some effective Ayurvedic drugs possessing Medopachaka, Agnideepaka, Vatanulomaka and Rasayana properties.

Therapeutic effect of an Ayurvedic drug in the management of Respiratory Allergic Disorders in children - A comprehensive review

Background: Respiratory allergic disorders (RAD) are the most common allergy symptom seen in children. Respiratory allergies are primarily caused by hyper responsiveness of the respiratory mucosa, which results in histamine release and mast cell activation. In Ayurvedic words, this could be viewed as an abnormality of the body's immune system or Ojus induced by disrupted Vata, Kapha, and Pitta. The prevalence of respiratory allergies in school-aged children in India varies widely, ranging from 5-20%. Methods: This study was completed by gathering the knowledge from classical Ayurvedic literature, research articles, guidelines and PubMed and MedLine databases. Results and Discussion: Dashmoola Katutrayadi drug is mentioned in Sahastra Yogam under Swasa Kasahara Kashaya have Shwas-Kasahar, anti-inflammatory, anti-histaminic and anti-asthmatic effects individually and in combinations. Conclusion: Present review reveals that these drugs have potential to alleviate the severity and frequency of RAD and can be used for the management of the same.

Effective Ayurvedic drugs after Virechana Karma in the management of Hypothyroidism: A Case Report

Hypothyroidism is a common endocrine disorder seen daily in hospital OPDs of Bhopal, in which the thyroid gland does not produce enough thyroid hormone. Levothyroxine is extensively used for the treatment of hypothyroidism but its treatment is unsatisfactory, considering its treatment is symptomatic and is for lifetime. Patients with hypothyroidism come to Ayurveda institutions seeking its treatment through Ayurveda because Ayurvedic management of hypothyroidism is considered much safe and effective and Ayurveda attempts to heal the root imbalance of hypothyroidism as compared to modern management. While analysing the signs and symptoms of hypothyroidism in Ayurvedic view, we note there is involvement of all Strotas. In the present case study, the patient came in the Panchakarma OPD with complaints of tiredness, Generalised weakness, Sudden weight gain, Puffiness of face, Hair fall, Constipation, Lack of concentration for last 2 year. Kanchanar Guggulu is the choice of drug for the study. Along with Aarogyavardhani Vati, Punarnava Mandur and Hanspadadi Kashayam as oral medicines after Virechana Karma done are advised. In this case study the effect of Ayurvedic management in hypothyroidism is evaluated.

Ayurvedic herbal formulations Haridra Khanda and Manjisthadi Kwath (brihat) in the management of allergic rhinitis: A pharmacological study

This study aims to pharmacologically validate Haridra Khanda (HK) and Manjishthadi Kwatham (brihat) (MMK) in allergy management using invivo and invitro studies to rationalize the prescription of these two ayurvedic polyherbal drug formulations, which are currently used in Indian government hospitals. Experimental animals received HK and MMK orally from day 0 to day 14 and histamine (1 mg/kg b.w/i.v) and 1 % evans blue (EB) (0.1 mL) via tail vein on day 14. The compound 48/80 (intracutaneous) challenged mice model followed the same technique. The former mimicked acute anaphylaxis and the latter mast cell degranulation. For both models, EB dye leakage was quantified spectrophotometrically to determine vascular permeability. Plasma histamine was measured in Compound 48/80-induced animals using LC-ESI-MS/MS. The guineapig received HK and MMK p.o. and 0.6 % histamine sprayed in a histamine chamber to simulate allergic rhinitis. Blood eosinophil count and sneeze rate were measured in histamine-challenged guineapigs. Goat R.B.C. membrane stability assay (mammalian cell membrane toxicity) and intracellular histamine-induced cytosolic Ca2+ release assay in Chinese hamster ovary (CHO) cells were performed in vitro. For both histamine and Compound 48/80 challenged animals, HK (22.81 % and 14.58 %) and MMK (19.71 % and 22.40 %) significantly reduced EB dye leakage (p < 0.05). Both formulations, HK and MMK considerably (p < 0.05) decreased plasma histamine (29.62 % and 25.37 % respectively) in mice and eosinophilic count (11.56 % and 9.94 % respectively) and sneeze rate (42.58 % and 29.03 % respectively) in guinea pigs. In membrane stability experiment, HK and MMK reduced RBC lysis. Both HK and MMK raw/dialysate blocked CHO cell cytosolic Ca2+ release. HK and MMK activities mimic mast cell stabilization with possible H1 receptor inactivation seen by decreased Ca2+ efflux and thus indicate potential for allergic rhinitis management. The combination of activities is usually related with curative and prophylactic therapy and might lead future clinical trials and therapies.