This study aims to recognize the influence of source- and path-effects on seismic spectra using the waveform data from the 2009 seismic activity, Harrat Lunayyir (HL), eastern flank of the Red Sea coastline, Saudi Arabia. This seismic activity is considered as a series of continuously evolving events reaching at some stage the maximum on 19 May 2009, with the largest shock of Mw 5.4, and then decayed. A total number of 218 events of magnitudes ML ≥ 2.0 were analyzed, which include the largest shock, 101 and 116 seismic events representing the seismic events within the early and decay stages, respectively. The data analysis using Frequency-time analysis revealed diversity patterns of frequency contents along the recorded seismic stations. Moreover, the modified WADATI diagram showed VP/VS variations of 1.7 ± 0.003 and 1.72 ± 0.002 before and after the occurrence of the largest-sized event among the HL seismic activity, respectively. Using the grid-search method to recover the space parameters representing the average source spectrum of each earthquake over the recorded seismic stations, estimates of source sizes and stress drops imply variations in self-similarity scaling relationship among the HL seismic activity. According to the results obtained from the current analyses, we propose a scenario began with an emplacement of seismic activity due to the local stress accumulation imports from an upward dike injection that is blocked by cooled and hardened magmatic materials in the upper crust from the earlier intrusions. The stress accumulation continued and was then released, at some stage, by the event having the maximum magnitude followed by seismic activities released due to stress relaxations.