It is still challenging to achieve the quantitative analysis with a spatially resolved measurement for a non-uniform fuel retention in Plasma Facing Materials (PFMs). In this work, a long-pulse (τ p ≈ 750 μs) Laser Induced Desorption-Quadrupole Mass Spectroscopy (LID-QMS) has been developed as a diagnostic approach for characterizing inhomogeneous deuterium (D) retention in tungsten (W) target exposed to D plasma for ∼1 h with the total ion flux of 4.0 × 1021 D/(m2 s) at the substrate temperature of 400 K. The behavior of desorption of trapped D during LID was analyzed. 2D-distribution features of D retention in the exposed and shadowed areas of the W-target were investigated with a lateral resolution of about 650 μm and depth resolution of approximately 175 μm. The results show that the D retention is almost uniform along lateral dimension in the D plasma exposure area with an average fluence of (2.51 ± 0.40) × 1020 D m−2, while the value in the shadow area is only (0.25 ± 0.04) × 1020 D m−2 which is one order of magnitude smaller than that in the exposure region. In the depth dimension, about 86.5% of the retained D is desorbed by the first laser pulse, which indicates that the D-retention is mainly distributed in the region less than 6.6 μm based on the diffusion length. The micro-structures of W target exposed to D plasma after the long-pulse laser irradiation present swelling, blistering as well as bubble-bursting, which might be attributed to the thermodynamic interaction due to the rapid release of D particles from bulk to surface of W target during LID.