Autoclave Chamber Research Articles

DISINFECTION BY HYDROGEN PEROXIDE AT LOW CONCENTRATION IN AIR: THE KEY ROLE OF CONDENSATION.

Gazeous hydrogen peroxide (H2O2) is commonly used for disinfection of room surfaces or sterilization of medical devices. Disinfection and sterilization processes are controlled by mean values measured at sterilizer chamber or room level. However, the surface phenomena (adsorption/ condensation) taking place on inoculum are essential and still not well-known. In the present study, a solution of water and H2O2 is sprayed in a room disinfection system (Glosair 400, ASP) for 12min. Condensation mass, H2O2 concentration, relative humidity (RH), macro zoom observations and inactivation kinetics of various microorganisms (Staphyloccocus aureus, Pseudomonas aeruginosa Candida albicans, Aspergillus niger) are reported. Macro-zoom observations reveal condensation and bubbling activities. Microbial inactivation is found optimal at low initial RH, corresponding to high H2O2 vapor concentration and low condensed mass. H2O2 concentration in the condensate is high and probably boosted by fractional condensation. In surface disinfection processes, inactivation of microorganisms occurs in presence of condensation although excessive condensation, due to high initial RH conditions or the presence of salt, decreases the microbial inactivation efficiency by dilution. While the present experimental conditions differ from those prevailing in H2O2 sterilization (59 % H2O2 under vacuum) or industrial disinfection processes (30% H2O2 at atmospheric pressure), they are partially transposable.

Effect of Water on Wear of DLC Coatings in High Temperature and Pressurized Ethanol

The friction and wear characteristics of a-C:H:Si and a-C:H coatings in an ethanol environment at 120 °C and 10 MPa focusing on the effects of water and dissolved oxygen in ethanol were investigated. The friction tests were conducted using an autoclave chamber, with gases (oxygen or nitrogen) and ethanol–water mixtures (0 and 6 vol.%). The wear acceleration of a-C:H:Si took place when it slid in ethanol with 6 vol.% water and pressurized by oxygen, thus the specific wear rate was the highest, approximately 1.8 × 10−7 mm3/Nm. The reason of this wear acceleration was assumed the effect of hardness reduction due to oxidation of the a-C:H:Si, so the O/C ratio by AES and the hardness of topmost surface by AFM nano-scratch were investigated. As a result, the higher O/C ratio showed higher specific wear rate due to the hardness reduction from 13.3 to 6.0 GPa. These findings highlight the role of water and dissolved oxygen in accelerating wear of a-C:H:Si coatings.Graphical abstract

DIELECTRIC BARRIER DISCHARGE BASED PLASMA DEVICE FOR COLD STERILIZATION IN WATER WITH ADDITIONAL ULTRASONIC CAVITATION

A prototype of plasma sterilizer based on dielectric barrier discharge (DBD) with additional ultrasonic cavitation for cold sterilization has been developed. It provides sterilization of reusable small dental instruments, and components made from corrosion-resistant metals and alloys (stainless steel, titanium, etc.), as well as surgical instruments, implants, small drills, plastic sundries, ozone resistant medical rubbers (silicone, etc.), polymers, epoxy resins and glass. The sterilizer is well suited for dentistry and can be used in medical, sanatorium, outpatient and other medical institutions, as well as in military field surgery. The volume of the sterilization bath is 2.5 l. The ozone concentration at the output of the ozone generator is 35…52 mg/l, the ozone concentration in the sterilizer chamber is 6.5…8 mg/l, depending on the water temperature and its volume in the bath. The dependence of the increase of ozone concentration in water on time at different oxygen flow rates is monitored.

Influence of loading on the heat transfer rate and specific water consumption of detached loosed fresh palm fruits during vertical mini-batch sterilization

This study presents the effect of loading on the heat transfer rate and specific water consumption of detached loosed fresh palm fruits during sterilization. Sterilization is an integral process of palm oil extraction that consumes a lot of water. However, most industrial research in the past focused on fresh fruit bunch (FFB) sterilization dynamics. At the same time, the medium- and small-scale industries sterilize already detached loosed fresh palm fruits (LFPF) that have been removed from the spikelet as purchased from aggregators. Therefore, the process data generated from the sterilization of FFB might not be optimal for sterilizing LFPF. This study, therefore, investigates the transient response of variation in LFPF loading of the sterilization chamber to steam injection during steam sterilization of LFPF at 3 ± 0.5 bars using a vertical, single-peak LFPF sterilizer that combines the boiler and sterilizer in a single unit. The LFPF were loaded at mass intervals of 0.2 kg ranging from 0.2 kg to 2 kg. The vapour temperature dynamics, heat transfer rate, steam energy utilization efficiency and specific water consumption parameters were studied. The average vapour temperature varied from 100 °C to 130 °C for the LFPF mass range of 0.2 to 2 kg at steam injection temperature of 140 °C and pressure of 3 ± 0.5 bars. This temperature range matched the saturated vapour temperature of pure water at 1.0––2.8 bars absolute pressure but slightly lower than the saturated vapour temperature of 133.54 °C for pure steam at 3 bars. The percentage of steam energy utilization effectiveness is inversely related to the steam mass flow rate. While energy effectiveness increased with loading mass, the steam mass flow rate decreased with increased LFPF mass. The value of the heat transfer rate ranged between 1.7––55.4 W, while the specific heat transfer rate ranged between 8.5–––41.8 W/kg. The average specific water consumption ranged from 0.014 to 0.09 kg-water/kg-LFPF, with an average value of 0.031 kg-water/kg-LFPF. This value is lower than 0.112–––0.5 kg-water/kg-FFB, with an average value of 0.256 kg-water/kg-FFB for conventional FFB steam sterilization. This represents an 87.9 % reduction in water consumption in conventional FFB steam sterilization. An empirical model deduced for specific heat transfer rate and water consumption for steam sterilization of LFPF at 3 ± 0.5 bars and vapour temperature range of 100––130 °C showed a high coefficient of determination above 99 %.

Analysis of Wet Pack Incidence in Steam Sterilization: A Study in a Chinese Medical Center.

BACKGROUND Central sterile supply departments (CSSDs) play a vital role in hospital infection control. We investigate the factors associated with wet pack occurrence after steam sterilization. MATERIAL AND METHODS We designed a log sheet to record information concerning sterilized packs. The data included the type of sterilized pack; outside weather (sunny, overcast, or rainy); the item in the sterilized pack; packaging material; whether the item had been packaged in compliance with guidelines; whether the pack had been laid flat, upright, or leaning at an acute angle; which sterilizer was used for sterilization of the pack; whether the pack had been placed on the top or bottom shelf inside the sterilizer chamber; whether the pack had been loaded in compliance with guidelines; the drying time following sterilization; and cooling time after sterilization. The sterilized packs in our study were selected from all of the packs that were sterilized in the CSSD of the authors' institution during June to December 2021. RESULTS Factors associated with wet pack occurrence after steam sterilization include: outside weather on the day of sterilization; the item in the sterilized pack; packaging material; whether the item had been packaged in compliance with guidelines; whether the pack had been placed on the top or bottom shelf; and cooling time after sterilization. Statistically significant differences (P<0.05) in wet pack incidence were identified for all of these factors. CONCLUSIONS Various factors are associated with wet pack occurrence after steam sterilization. Recommendations for reducing the risk of wet packs include regular maintenance of the steam pipeline, regular replacement of thermal insulation materials for the steam pipeline, and extension of the drying time.

Применение автоматического парового стерилизатора для обеззараживания отходов иммунобиологического производства, контаминированных патогенными биологическими агентами

One of the ways to neutralize immunobiological production waste is their treatment in sterilizers with saturated water vapor under pressure. Currently, medical and pharmaceutical industries and laboratories use sterilizers with sterilization and disinfection programs preset by manufacturers. Decontamination modes are defined in Federal sanitary rules, norms and hygienic standards, SanPiN 3.3686-21, while the parameters of preliminary phases of the cycle must be tested experimentally and validated for specific conditions. Within the frames of this study, we have tested the modes of disinfection of an automatic steam sterilizer by the example of waste products from the production of cholera vaccine and anti-rabies immunoglobulin manufactured by the FSSI Russian Anti-Plague Institute «Microbe» of the Rospotrebnadzor. The wastes are used nutrient media, carcasses of laboratory animals (rabbits) and consumables. The chamber was loaded with «clean» objects in quantity and configuration, repeating the loading of material with pathogenic biological agents (PBA). The mode of inactivation inside the chamber and the objects was controlled by physical, chemical and biological methods. Based on the results of the experiments, corrective actions were taken to change the program and design of containers for autoclaving. It was concluded that the default disinfection programs set by manufacturers do not fully take into account specific conditions and need to be adjusted with further validation. It is necessary to develop standard operating procedures for personnel involved in the decontamination of pathogenic materials, in which should be stated the order of placement of objects and the means of control in the containers with PBA and the sterilizer chamber. Keywords: steam sterilizers, pathogenic biological agents, disinfection with saturated water vapor, phases of the steam disinfection cycle, validation.

High-Pressure FDM 3D Printing in Nitrogen [Inert Gas] and Improved Mechanical Performance of Printed Components

Fundamentally, the mechanical characteristics of 3D-printed polymeric objects are determined by their fabrication circumstances. In contrast to traditional polymer processing processes, additive manufacturing requires no pressure during layer consolidation. This study looks at how a high-pressure autoclave chamber without oxygen affects layer consolidation throughout the fused deposition modelling process, as well as the mechanical qualities of the products. To attain high strength qualities for 3D-printed components such as injection-molded specimens, an experimental setup consisting of a 3D printer incorporated within a bespoke autoclave was designed. The autoclave can withstand pressures of up to 135 bar and temperatures of up to 185 °C. PLA 3D printing was carried out in the autoclave at two different pressures in compressed air and nitrogen atmospheres: 0 bar and 5 bar. Furthermore, injection molding was done using the same PLA material. Tensile, flexural, and Charpy tests were carried out on samples that were 3D printed and injection molded. In nitrogen, oxidation of the environment was prevented by autoclave preheating before printing, and autoclave pressure during printing considerably promotes layer consolidation. This imprinted mechanical strength on the 3D-printed items, which are virtually as strong as injection-molded components.

SG-APSIC1130: Zero wet pack Pimporn Sirikraiwattanawong, Thailand

Objectives: We noted moisture in Thompson retractor sets after steam sterilization in our hospital. Moisture can cause severe problems leading to potentially contaminated instruments that carry infection risk to patients and cause procedure delays, wasted time and effort, greater workload, and higher costs. We sought to reduce the number of retractor sets with moisture to zero. Methods: The central sterile supply (CSS) team discussed the cause of the problem. We hypothesized that temperature difference between the sterilizer chamber and inside the container might create condensation and thus moisture in the final surgical set. We collected and analyzed data and proposed an experiment to improve the sterilization process. We performed a trial of sterilization process improvements pertaining to proper loading technique and the packaging process. We also evaluated the appropriate drying time for rigid containers. We then rearranged the process and adjusted the cooling time from 30 to 60 minutes after steaming. Results: Moisture in Thompson retractor packs occurred because of thicker, rigid containers. We removed the previous type of lining material to separately steam the rigid surgical instrument, and we extended the cooling time to 60 minutes. We updated standard operation procedures and continued to monitor and re-evaluate the process. Conclusions: We identified the primary cause of moisture in Thompson retractor sets after steam sterilization. We illustrated that avoiding sterilizer overload, avoiding contact with fabric wrapping materials, and proper cooling time kept the pack moisture free. Occurrences of moisture in surgical packs after sterilization should be reported and handled efficiently by CSS personnel to preserve quality and avoid waste.

ПРОЕКТИРОВАНИЕ УСТАНОВКИ ПО ПЕРЕРАБОТКЕ МЕДИЦИНСКИХ ОТХОДОВ ТЕРМОХИМИЧЕСКИМ МЕТОДОМ

The article deals with issues related to the development of the design of a medical waste processing machine based on the thermochemical method, special attention is paid to the cutting equipment used in the machine. The problem statement is formulated as follows: to design a machine that allows realizing the possibility of processing medical waste by the thermochemical method; offer design options for the cutting equipment of the machine and justify the choice of knife material based on finite element modeling. The design of the machine is proposed, consisting of a base on which an electric motor is located, the movement from which is transmitted by means of a V-belt transmission to the tooling drive. Processing and disinfection of medical waste is carried out in a sterilization chamber. The sterilization chamber and the tank associated with it are structurally the most complex. The main structural elements of the tank are the base, the sterilization container and the lid. Direct processing of medical waste is carried out by rotating the rotary assembly with tooling. In the proposed machine tooling includes four movable horizontal and two vertical knives. During the processing of medical waste, the working surfaces of horizontal knives are exposed to a number of factors that lead to their intensive wear. The article offers several designs of knives to increase their durability. In order to check the correctness of the choice of material and the influence of operating conditions, a FE model was developed for the process of heating a knife under the action of a friction force caused by polyethylene chips descending along the front surface. Calculations were made for two knife materials: carbon steel and gray cast iron. The simulation results showed that, according to a number of criteria, gray cast iron is the most preferred knife material. The use of cast iron will ensure the rapid achievement of the required temperature in the sterilization chamber, and lower internal stresses will reduce the likelihood of knife breakage. To improve the reliability of the machine, design options for movable knives that are subject to the most intense wear are given.

ОПРЕДЕЛЕНИЕ МОЩНОСТИ УСТАНОВКИ РОТОРНОГО ТИПА ПО ПЕРЕРАБОТКЕ МЕДИЦИНСКИХ ОТХОДОВ

The article considers the rationale for the required power of rotary-type machines for the processing of medical waste. The problem statement is formulated as follows: to develop an analytical method for calculating the power of rotary devices for waste processing. An analysis of the morphological and structural composition of medical waste made it possible to identify enlarged groups of waste, for the processing of which both cutting with a blade and crushing are used. The calculation procedure was performed for a rotary system consisting of a traverse carrying four horizontal and one U-shaped vertical knives. Due to the peculiarity of the medium being processed (medical waste), it is impossible to use the theoretical and practical experience gained during processing with cutters with chip removal from the surface of the material. The value of the critical cutting force depends on the sharpness of the blade and the breaking contact stress, which can be determined through the reduced breaking contact stress for a group of materials subject to cutting failure. When determining the critical cutting force, it must be taken into account that not the entire length of the blade is involved in the cutting process, since the medical waste loaded into the sterilization chamber has a low bulk density, which changes with time. In the presented technique, the power spent on grinding by cutting is found by integrating along the length of the cutting edge and taking into account the presence of two cutting blades. In addition to the power spent on grinding, in the process of cutting with a blade, there are power losses associated with the movement of the cut material along the bevels of the blade, depending on the thickness of the cut layer, the modulus of elasticity and the Poisson's ratio of the material being processed. The proposed method takes into account that the grinding of brittle materials that make up medical waste is carried out during processing not by cutting, but by crushing. Since the mixing process is combined with the grinding process during the processing of medical waste, the rotating rotor plays the role of a paddle mixer. In turn, the power expended on mixing depends on the resistance force acting on the elementary area of the blade, as well as on the rotation speed. Thus, in the processing of medical waste, the total power costs are defined as the sum of the power costs for grinding and mixing. The obtained analytical expressions make it possible at the design stage not only to choose the rational drive power, but also to justify the variable rotor speed.

Antibacterial Applications of Low-Pressure Plasma on Degradation of Multidrug Resistant V. cholera

The existence of Vibrio cholera (V. cholera) is a major health problem in many parts of the world; therefore, the treatments of V. cholera have always remained necessary for public safety, health, and environmental protection. In the last few decades, plasma discharges have proven to be a novel technique of sterilization against infectious bacteria such as V. cholera. In this research, a low-pressure plasma (LPP) technique has been introduced for the degradation of multidrug resistant V. cholera. The V. cholera strains with 107 CFUs (colony-forming units) were treated by low-pressure plasma, with and without H2O2 injection into the sterilization chamber, to investigate and report the adverse effects of plasma on V. cholera. The results demonstrated that plasma treatment has significant effects on the degradation of V. cholera in the presence of H2O2 vapors inside the plasma sterilization chamber. The time-course study of the bactericidal effects revealed that there is no regeneration or increase in the number of V. cholera colonies after plasma treatment.

Evaluation of Virucidal Efficacy of Cold Plasma on Bacteriophage Inside a Three-Layered Sterilization Chamber

Traditional disinfection methods against pathogens have numerous shortcomings, and inventive methods like cold plasma are required for virus inactivation. Here, an atmospheric pressure 13.56 MHz radio-frequency hollow cathode (RF–HC) cold plasma device has been used to build a cold plasma sterilization device, and its virucidal activity is assessed against P2 bacteriophage, a model surrogate for pathogenic viruses. The heart of this device contains a three-layered sterilization chamber which is a rectangular parallelopiped of 42 × 32 × 30 cm3. Optimization experiments were performed to make each corner of this chamber completely virus free after cold plasma treatment. This two-pronged study was conducted to establish the requirement of minimum vol. % H2O2 in minimum time for the complete elimination of phages inside this sterilization chamber even when plasma is not in the direct line of sight. In initial experiments, the effect of the direct plasma line of sight was seen as the top and bottom layers showing less phage killing as compared to the middle of the sterilization chamber. Complete sterilization of bacteriophage, in all the three layers inside the sterilization chamber, was achieved by plasma treatment with 6% H2O2 for 10 min in 80 watts of plasma operating power. It was also seen that 6% H2O2 mist alone is not sufficient to provide a high degree of sterilization, and normal water mist combined with cold plasma can provide a higher level of sterilization at each corner of the chamber.

Planning Implications Related to Sterilization-Sensitive Science Investigations Associated with Mars Sample Return (MSR).

Planning Implications Related to Sterilization-Sensitive Science Investigations Associated with Mars Sample Return (MSR).

Aplikasi Termokopel untuk Pengukuran Suhu Autoklaf

Autoclave is a tool used to sterilize medical instruments such as surgical knives and scissors. The process of autoclave sterilization is to provide heat energy to the autoclave. Hot steam will rise into the closed chamber. The control system will monitor the temperature until the required temperature is reached (121-124 °C). Temperature measurement in the autoclave is done using a thermocouple sensor. Temperature testing is done by comparing temperature measurements using a thermocouple and a mercury thermometer. Testing with a thermocouple produces output that is linear and comparable to measurements using a mercury thermometer. The correlation coefficient value of the measurement results using a mercury thermometer is 0.99982 and a thermocouple is 0.99894. Furthermore, the test is carried out by looking at the change in the line on the indicator tape to the length of time for sterilization in the autoclave chamber. As a result, the longer the sterilization time, the change in the line on the indicator tape will look darker, which indicates the sterilization process is going well.Keywords: temperature measurement, autoclave, type K thermocouple, indicator tape

Studies on the curing, tensile and adhesion characteristics of cushion gum to carcass in truck retreaded tires: the effect natural rubber types

In order to improve the quality of truck retreaded tyres, the cushion gum (CG) plays an important role as an adhesion medium to bond the carcass of the new tread to the worn old tyre. This study investigates the effect of natural rubber types such as reclaimed skimmed latex (RSL), ribbed smoke sheet (RSS), chloroprene (CR) and RSS added 10 phr of carbon black (CB) towards its CG performance in cold cure systems. The CG was compounded based on ASTM D3182 with 10 phr of Cumaroun Resin, 5 phr of Calcium Silicate, and other chemicals. The 4 types of CG were tested its physical and tensile properties. To determine the curing time, the rheological was analysed. The results show that the addition of carbon black may improve physical and tensile properties, excluding the elongation at break. It is found that the curing time of cushion gum based on SL rubber has the shortest duration. Each CG was applied to retreat the same type of truck’s worn tyres (1000R20). After surface modification, 5 mm of CG was bonded new same carcass the old surface and continued by heating in the autoclave chamber for 3 hours with pressure at 2 atm. The results show that the adhesion strength of cushion gum based on RSL; RSS; CR; RSS/CB is as follows: 13.64; 8.21; 8.04; 8.01 kN/m respectively, while the adhesion strength of commercial cushion gum is 15,38 kN/m. The spectra FTIR of each cushion gum convince that the less dispersion matrix compared to commercial cushion gum. To sum up, the RSL-NR is the recommended polymer for cushion gum compounding. However, in order to improve its quality, it is suggested to do some pre-treatment and increase the number of tackifier and processing oil in the formulation

Properties and microstructure of eco-friendly alkali-activated slag cements under hydrothermal conditions relevant to well cementing applications

Alkali-activated slag (AAS) cements have the potential to be used as eco-friendly sealant materials for oil well cementing applications. Sodium carbonate and calcium carbide residue were applied as the solid alkali activators for preparing the AAS binders in this work. These binders were exposed to the hydrothermal curing at 120 °C and 21 MPa, which was employed in the autoclave chamber with water soaking to simulate the downhole stress conditions at medium-range temperature. The compressive strength, linear volume change and water permeability of the hydrothermally cured binders were tested, and a multi-technique approach was utilized to investigate the microstructure evolution. The results showed that the compressive strength development of binders was sufficient for the target well cementing application according to the American Petroleum Institute standard (above 14 MPa). After 28 days of hydrothermal curing, the binders exhibited favorable volume expansion of 0.0803-0.2007% and water permeability of 5.07×10-4-8.61×10-3 mD. The main binding gel phase of C-A-S-H formed in the binders gradually converted to highly crystalline hibschite (Ca3Al2(SiO4)1.25(OH)7), accompanied by the rapid alkalis (Na) leaching from the matrix due to water soaking. Nevertheless, the hydrothermally cured AAS binders still displayed excellent structure integrity. This study confirms that the eco-friendly AAS binders perform satisfactorily for the oil well cementing operations.

STERILIZER CHAMBER DESIGN WITH TELEGRAM-BASED INTERNET OF THINGS (IoT) APPLICATIONS

In order to reduce the spread of the corona virus in office areas, malls or housing, a sterilizer chamber is installed. It is used to detect someone who will enter an area and he must be sterilized first by spraying the entire body with disinfectant liquid automatically. By combining the sterilizer chamber tool with IoT technology, the sterilizer chamber tool can be monitored continuously at a certain distance using a sensor as a detector which will provide information to one of the operators of the tool condition, about the remaining liquid, the number of people who have been sterilized. The system works by providing information on the liquid spray time of about 5 seconds and information on the liquid level in the tube from level 5-70. The average output pin voltage is 4.89 V when the sensor detects an obstacle in front of the sensor and the NODEMCU will receive a voltage 4.89 V from the output pin and ultrasonic sensor HC-SR04. The highest voltage reaches 673.2 Mv, when the distance to the object is 40 cm with the lowest voltage is 100.3 mV with the closest distance being 5 cm.

Growth performance and mineral analysis of Pleurotus ostreatus from various agricultural wastes mixed with rubber tree sawdust in Malaysia

This study aims to contrast the impacts of various agricultural wastes on the development, yield and mineral content of P. ostreatus. All substrates were added 5% by weight dry wheat bran and 1.5% by weight dry calcium carbonate and sterilized at 100 °C for 8 h using a sterilization chamber. The best substrate to obtain a greater total weight of fresh mushrooms and biological efficiency was 50% SB + 50% SR (207.87 g/bag; 78.44%) with mycelial growth at 26 ± 2 days. All of the agricultural wastes used in this study have abundant potassium (K) content as it is the mineral element needed for the mushroom growth. Therefore, the potassium (K) was the maximum macroelement in concentration as compared to other nutrients and all heavy metal content were not detected in the first fresh mushroom harvested. Therefore, SB seemed to be a better alternative material to grow P. ostreatus.

Design Thinking as a Framework for the Design of a Sustainable Waste Sterilization System: The Case of Piedmont Region, Italy

The development of new methods for the correct disposal of waste is unavoidable for any city that aims to become eco-friendly. Waste management is no exception. In the modern era, the treatment and disposal of infectious waste should be seen as an opportunity to generate renewable energy, resource efficiency, and, above all, to improve the population’s quality of life. Northern Italy currently produces 66,600 tons/year of infectious waste, mostly treated through incineration plants. This research aims to explore a more ecological and sustainable solution, thereby contributing one more step toward achieving better cities for all. Particularly, this paper presents a conceptual design of the main sterilization chamber for infectious waste. The methodology selected was Design Thinking (DT), since it has a user-centered approach which allows for co-design and the inclusion of the target population. This study demonstrates to the possibility of obtaining feasible results based on the user’s needs through the application of DT as a framework for engineering design.

Nine Channel Temperature Data Logger in Measuring the Effectiveness of the Sterilization Process of Medical Instruments with Dry Sterilization

Measuring the temperature on the dry sterilizer is very necessary because the temperature inside the dry sterilizer has the possibility that the temperature is not the same as the temperature that has been set and is displayed on the display. If the temperature in the dry sterilizer does not match the standard setting temperature for the sterilization process, then the sterilization process is said to be imperfect The purpose of this study is to record and monitor whether the distributed temperature in the sterilization chamber corresponds to the setting temperature. The workings of the temperature data logger tool is that the type K thermocouple temperature sensor will detect the temperature which then enters the analog signal conditioning circuit which then enters the ATMegga 2560 which has been given a program and processed in such a way, then the temperature will be displayed on a 4x20 character LCD. Temperature measurement data will be saved to the SD Card every 10 seconds in the form of a TXT file. This research has been used to record 2 sterilizers and compared with the Madgetech OctTemp2000 data logger. Based on data measurements and comparisons, the average error was obtained at a temperature of 50ºC with the smallest error value of 0.7% and the largest value of 3.9%. At a temperature of 100ºC, the smallest error value is 1.6% and the largest is 10.5%. Then at a temperature of 120ºC the smallest error value is 0.0% and the largest is 8.5%. This research can be used to help analyze the distribution of temperature in a room. With these measurement results, it can be said that this study still has afairly high error value at several measurement points.