Au Nanocomposites Research Articles

Enhancing the Efficiency of Doxorubicin -Conjugated with gold nanocomposites for Active Targeting and Laser Treatment for Human Esophagus Cancer Cells

Nanotechnology is being used in cancer treatment to deliver drugs to tumors, improve imaging, and detect cancer cells. Nanoparticles are being used to deliver drugs more effectively to tumors, while also reducing the side effects of the drugs. The current context shows the conceivable biomedical utility of gold nanoparticles (AuNPs) covered with doxorubicin (DOX) for double chemotherapy and photothermal therapy (PTT) of throat cancers (esophagus) in vitro. DOX as FDA-authorized anti-cancer drug was added to AuNPs to enhance the chemotherapy impact. The blended DOX - AuNPs showed exorbitant cytotoxicity at the cancer cells and strong light absorption for temperature expansion in vitro. In the wake of treating DOX– Au nanocomposites with inside the esophagus cell line, PTT-helped DOX-Au nanocomposites involved the fantastical cytotoxicity of the cell strains for 15 min after the laser treatment. Exceptionally cytotoxicity impact in esophagus cell line turned out to be more attractive due to specific light absorption with the aid of using the directed DOX-Au nanocomposites. Thusly, the proposed DOX-Au nanocomposites might be an ability nano-theranostic material for treating throat cancers. The gene expression levels withinside the esophagus cell line from every group with or without laser exposure have been compared individually with control group. Bax has been overexpressed in esophagus cell line organization post 15 min laser irradiation. Conversely, BCL-2 gene has been down regulated with the aid of using RT-PCR analysis post 15 min laser irradiation, while in comparison to control cell line.

Role of Defects of Carbon Nanomaterials in the Detection of Ovarian Cancer Cells in Label-Free Electrochemical Immunosensors.

Developing label-free immunosensors to detect ovarian cancer (OC) by cancer antigen (CA125) is essential to improving diagnosis and protecting women from life-threatening diseases. Four types of carbon nanomaterials, such as multi-wall carbon nanotubes (MWCNTs), vapor-grown carbon fiber (VGCFs), graphite KS4, and carbon black super P (SP), have been treated with acids to prepare a carbon nanomaterial/gold (Au) nanocomposite. The AuNPs@carbon nanocomposite was electrochemically deposited on a glassy carbon electrode (GCE) to serve as a substrate to fabricate a label-free immunosensor for the detection of CA125. Among the four AuNPs@carbon composite, the AuNPs@MWCNTs-based sensor exhibited a high sensitivity of 0.001 µg/mL for the biomarker CA125 through the square wave voltammetry (SWV) technique. The high conductivity and surface area of MWCNTs supported the immobilization of AuNPs. Moreover, the carboxylic (COO-) functional groups in MWCNT improved to a higher quantity after the acid treatment, which served as an excellent support for the fabrication of electrochemical biosensors. The present method aims to explore an environmentally friendly synthesis of a layer-by-layer (LBL) assembly of AuNPs@carbon nanomaterials electrochemical immunoassay to CA125 in a clinical diagnosis at a low cost and proved feasible for point-of-care diagnosis.

Au and zincite assisted silica-titania nanocomposite coated fiber optic pH sensor: Structural thermal, and optical characteristics

Accurate pH evaluation is required in different fields including food science, agriculture, environmental monitoring, and medical research. The low-temperature sol-gel method is employed to synthesize the Au and zincite-assisted silica-titania nanocomposites for the pH assessment. A mixture of indicator dyes (phenol red, creosol red, phenolphthalein, and bromophenol blue) is encapsulated in mesoporous nanocomposites and coated on fiber optic. Crack-free, dense, and porous surface with low roughness and high thermal stability (≤500 °C) is observed for all three nanocomposites. Low roughness 4 nm, low refractive index 1.5, and high surface area 333 m2/g DST nanocomposite exhibited sensitivity 28.3 counts/pH which is greater than 17.5 counts/pH (DAuST) and 14 counts/pH (DZST). The fiber pH devices possessed reversible, stable, and fast response 0.19 s into pH 12 (DST), DZST ∼ 0.18 s, and DZST ∼ 0.22 s, without any leaching.

Gold embedded gum ghatti grafted polyacrylamide nanocomposite for in vitro release study of curcumin

Nanoparticle systems are highly fascinating because of their unique physicochemical properties. Gold nanoparticles (Au NPs) can be applied in medical applications due to its biocompatibility and chemical nontoxicity. In this work, gold-embedded gum ghatti-grafted polyacrylamide (Gg-g-AM(Au)) nanocomposite was synthesized via an in situ polymerization method. The synthesized Gg-g-AM(Au) nanocomposite was characterized by using various techniques such as UV–visible spectroscopy, FTIR, XRD and FESEM. The Gg-g-AM(Au) nanocomposite was used for in vitro release of curcumin at pH 1.2 and 7.4. To evaluate drug release mechanisms and kinetics, the release data were fitted to zero order, first order, Korsmeyer-Peppas, and Higuchi models. The antibacterial behavior of the nanocomposite systems was also investigated. The Gg-g-AM(Au) nanocomposite systems can be considered as a good carrier towards in vitro release of the curcumin.

Enhanced transmembrane electron transfer in Shewanella oneidensis MR-1 using gold nanoparticles for high-performance microbial fuel cells.

Low efficiency of extracellular electron transfer (EET) is a major bottleneck in developing high-performance microbial fuel cells (MFCs). Herein, we construct Shewanella oneidensis MR-1@Au for the bioanode of MFCs. Through performance recovery experiments of mutants, we proved that abundant Au nanoparticles not only tightly covered the bacteria surface, but were also distributed in the periplasm and cytoplasm, and even embedded in the outer and inner membranes of the cell. These Au nanoparticles could act as electron conduits to enable highly efficient electron transfer between S. oneidensis MR-1 and electrodes. Strikingly, the maximum power density of the S. oneidensis MR-1@Au bioanode reached up to 3749 mW m-2, which was 17.4 times higher than that with the native bacteria, reaching the highest performance yet reported in MFCs using Au or Au-based nanocomposites as the anode. This work elucidates the role of Au nanoparticles in promoting transmembrane and extracellular electron transfer from the perspective of molecular biology and electrochemistry, while alleviating bottlenecks in MFC performances.

Bioinspired GO/Au nanocomposite synthesis: Characteristics and use as a high-performance dielectric material in nanoelectronics

• O/Au nanocomposites were successfully synthesised using Ocimum basilicum . (basil) seed extract. • The GO/Au nanocomposite can also be used to demonstrate high-performance electronic devices. • The bioinspired approach enhances the dielectric behaviour of GO/Au nanocomposite matrixes by adding organic macromolecules capable of modest GO reduction. A bioinspired method was used to synthesise graphene oxide (GO) based noble metal (Au) nanocomposite (GO/Au nanocomposite) using chemical exfoliated graphene oxide as the base matrix and biosynthesised gold (Au) nanoparticles. The morphological characterization of GO and the GO/Au nanocomposite was determined using TEM and SEM analysis. The amount of Au nanoparticles conjugated with GO was determined using an EDAX analysis. The absorption and emission properties of GO and GO/Au nanocomposite are revealed through optical studies. FTIR analysis revealed the presence of different functional groups in GO and GO/Au nanocomposite, while XRD analysis confirmed the formation of crystalline GO and GO/Au nanocomposite of different sizes. TGA analysis was used to determine the degradation behaviour of the two products with respect to temperature. An LCR analysis was used to characterise the electrical characteristics of GO dielectric features as a function of frequency between 1 Hz and 1 MHz. The dielectric permittivity and electrical conductivity of GO were very frequency-driven. The results demonstrated that GO has direct current and correlated barrier hopping conductivity processes in the low and high frequency bands. The dielectric constant and loss factors of the GO/Au nanocomposite show that the bioinspired approach includes organic macromolecules capable of modest GO reduction and so modifying the C/O ratio, resulting in an enhancement in the matrix's dielectric characteristics. This study further demonstrates that high-performance electronic devices, as well as electrical and energy storage systems based on high dielectrics, might be scaled using GO-based materials.

Multifunctional Au Modified Ti3C2-MXene for Photothermal/Enzyme Dynamic/Immune Synergistic Therapy.

Ti3C2-MXene-based composites provide multifunctional interfaces in diagnosis and treatment of tumors. Herein, we proposed a multifunctional nanoplatform based on Ti3C2-MXene-Au nanocomposites, which combines photothermal properties and peroxidase-like activity, accomplishing synergistic photothermal therapy (PTT) and enzyme dynamic therapy (EDT) accompanied by photoacoustic (PA) and thermal dual-mode imaging in vivo. Furthermore, PTT induces immunogenic cell death, and EDT promotes cell apoptosis, facilitating dendritic cell (DC) maturation and T cell infiltration into the tumor. On this basis, the antibody OX40 (αOX40) was utilized to further contribute immune therapy for reversing the immunosuppressive tumor microenvironment by activating CD4+ and CD8+ T cells. In summary, a triune of PTT/EDT/antitumor immune therapy is achieved by combining Ti3C2-MXene-Au nanocomposites and αOX40, which possesses several strong features of good biocompatibility, NIR-controlled targeting, significant cancer cell killing, and satisfactory biosafety in vitro and in vivo. Our work might highlight the promising application of MXene-based nanoplatforms for cancer therapy.

Laser-assisted synthesis of Si–Au nanocomposites with trisodium citrate and their enhanced nonlinear optical properties

Laser-assisted synthesis of Si–Au nanocomposites with trisodium citrate and their enhanced nonlinear optical properties

Chitosan-G-Glycidyl Methacrylate/Au Nanocomposites Promote Accelerated Skin Wound Healing.

Herein, we report the synthesis of Au nanoparticles (AuNPs) in chitosan (CTS) solution by chemically reducing HAuCl4. CTS was further functionalized with glycidyl methacrylate (chitosan-g-glycidyl methacrylate/AuNP, CTS-g-GMA/AuNP) to improve the mechanical properties for cellular regeneration requirements of CTS-g-GMA/AuNP. Our nanocomposites promote excellent cellular viability and have a positive effect on cytokine regulation in the inflammatory and anti-inflammatory response of skin cells. After 40 days of nanocomposite exposure to a skin wound, we showed that our films have a greater skin wound healing capacity than a commercial film (TheraForm®), and the presence of the collagen allows better cosmetic ave aspects in skin regeneration in comparison with a nanocomposite with an absence of this protein. Electrical percolation phenomena in such nanocomposites were used as guiding tools for the best nanocomposite performance. Our results suggest that chitosan-based Au nanocomposites show great potential for skin wound repair.

Preparation and Characterization of Chitosan and Inclusive Compound-Layered Gold Nanocarrier to Improve the Antiproliferation Effect of Tamoxifen Citrate in Colorectal Adenocarcinoma (Caco-2) and Breast Cancer (MCF-7) Cells.

Cancer diseases have been linked to a huge number of causes that led to deaths in this century along with cardiovascular and lung diseases. Most death-leading types of cancer are colon, lung, breast, and prostate cancers. Due to the remarkable properties of gold (Au) nanocarrier, they are used to deliver and improve tamoxifen (Tam) citrate activity in Caco-2 and MCF-7 cells. In this study, preparation of Au nanoparticles (NPs), zeta-potential and size, high resolution transient electron microscopy (HRTEM), high-performance liquid chromatography, ultraviolet-visible spectra, fluorescence microscopy, fourier infrared spectroscopy, and real-time cellular analysis xCELLigence technology were investigated. The zeta-average size of the Tam- β-cyclodextrin (β-CD)-hyaluronic acid (HA)-chitosan (Chi)-Au nanocomposite is 82.02 nm with a negative zeta potential of -23.6. Furthermore, HRTEM images showed that, successful formulation of polymer shell around Au core and the Au NP shape is mostly spherical, triangle and irregular. Furthermore, the fluorescence microscope image showed proper cellular uptake of the Tam-β-CD-HA-Chi-Au nanocomposite in MCF-7 and Caco-2 cells. Additionally, Tam-β-CD-HA-Chi-Au nanocomposite significantly improved the cytotoxic activity of Tam citrate on Caco-2 cells. IC50 value of Tam reduced from 8.55 µM to 5.32 µM, after 48 h of incubation time (p value <0.00001). This study showed that Tam-β-CD-HA-Chi-Au nanocomposite is a potential nanocarrier for delivering the drug to Caco-2 and MCF-7 cancer cells, since it has improved Tam citrate activity on colorectal cancer cells. After all, the developed formula showed more effect on Caco-2 than MCF-7. The prepared nanocomposite could be used to improve the cancer therapy in clinical trials.

Gold‐Nanoparticles‐Decorated ZrO2‐CuO Nanocomposites: Synthesis, Characterization and A Novel Platform for Electrocatalytic Formaldehyde Oxidation

AbstractIn this study, gold nanoparticles decorated copper oxide‐ zirconium oxide mixture has been deposited on glassy carbon electrode surface by simple electrochemical technique. The obtained composite surface has been investigated by electrochemical impedance spectroscopy, scanning electron microscopy, energy dispersive X‐ray spectroscopy, X‐ray diffraction spectroscopy, X‐ray photoelectron spectroscopy in detail. The results of SEM images, XPS and XRD data indicated the surface of ZrO2‐CuO composite was successfully decorated with Au nanoparticles. The resulting ZrO2‐CuO/Au nanocomposite contained about 1.48 % Au nanoparticles with 70 nm average diameter. Then, the catalytic performance of ZrO2‐CuO/Au nanocomposites was investigated by using formaldehyde oxidation. The comparative studies have been revealed that the ZrO2‐CuO/Au nanocomposites exhibited a better current density towards formaldehyde oxidation than that of bare, ZrO2‐CuO, and Au modified electrodes in alkaline media. According to the best of our knowledge, this is the first study dealing with the usage of ZrO2‐CuO/Au nanocomposites in formaldehyde oxidation. As the electrochemically fabricated, stable ZrO2‐CuO/Au surface improved catalytic activity towards formaldehyde oxidation, this material is a promising candidate for application in fuel cells and removal of formaldehyde from aqueous solutions.

Thermo-responsive and reversible colorimetric sensors as fever-checkers using low critical solution temperature polymer–Au nanocomposites

Hydrogels have proven to be among the best substrates for thermo-responsive patches owing to their hydrophilic functional groups. Poly(N-isopropylacrylamide) (PNIPAM) is a common thermo-responsive polymer with a low critical solution temperature (LCST). This polymer changes its phase in response to the solution temperature. In this study, a thermo-responsive colorimetric sensor was fabricated by combining Au nanoparticles (AuNPs) with PNIPAM hydrogels to alter the interparticle spacing of AuNPs contained in the polymer matrix in response to temperature stimuli. The as-prepared PNIPAM@Au hydrogel demonstrated excellent reversible swelling–shrinking capabilities when the temperature changed from 25° to 50°C in 1 min. As the hydrogels shrank, water was released near the shrinking hydrogel. The color of the hydrogel changed from red to purple. This color shift revealed that the localized surface plasmon resonance band red-shifted as the interparticle distance of the AuNPs in the PNIPAM@Au hydrogel was reduced owing to the coupling effect of each AuNP. The thermo-responsive and colorimetric characteristics of the PNIPAM@Au hydrogel may be useful in monitoring body temperature changes in infants with high fever.

Semiconductor Metal Oxides Doped with Gold Nanoparticles for Use in Acetone Gas Sensors

The effect of nanosized gold particles on the adsorption-sensitive properties of SnO2–Au sensors under the detection of acetone vapors has been studied. Different techniques for the preparation of SnO2–Au nanocomposites with an average Au particle size of 2 nm were applied. It has been found that a fivefold increase in the sensor response to acetone vapors and threshold sensitivity (C lim) of 0.1 ppm are achieved by adding gold to tin dioxide in the colloidal form during synthesis. While adding gold in ion form (Au (III)) leads to a growth of the sensor response to acetone vapors by 2.7 times and defines C lim of 0.2 ppm. The slope of the calibration curves of the SnO2–Au sensors allows registering acetone vapors at concentrations ranging from C lim to 5 ppm. This concentration range can be used for express diagnostics in diabetes. The enhanced sensitivity of SnO2–Au sensors to acetone vapors can be explained by an increase in the adsorption-catalytic activity of tin ions as a result of the modifying effect of sulfate groups and the envolving of highly dispersed gold in the adsorption – catalytic process of oxidation of acetone molecules.

Synthesis of multi-branched Au nanocomposites with distinct plasmon resonance in NIR-II window and controlled CRISPR-Cas9 delivery for synergistic gene-photothermal therapy

Clinical implementation of photothermal therapy (PTT) is mainly hampered by limited tissue penetration, undesirable thermal damage to normal tissues, and thermotolerence induced by heat shock proteins (HSPs). To overcome these obstacles, we constructed a novel gene-photothermal synergistic therapeutic nanoplatform composed of a multi-branched Au nanooctopus (AuNO) core and mesoporous polydopamine (mPDA) shell, followed by CRISPR–Cas9 ribonucleoprotein (RNP) loading and then polyethylene glycol-folic acid (PEG-FA) coating. AuNO was simply synthesized by adjusting the ratio of cetyltrimethylammonium chloride (CTAC) and cetyltrimethylammonium bromide (CTAB), which showed significant localized surface plasmon resonances in the NIR-II window, and exhibited an excellent tissue penetration capability and high photothermal conversion efficiency (PCE, 47.68%). Even, the PCE could be further increased to 66.17% by mPDA coating. Furthermore, the sequential modification of AuNO@mPDA using RNP and PEG-FA can down-regulate HSP90α expression at tumor sites, enhance apoptosis and reduce the heat resistance of cancer cells. The synergistic effect of enhanced photothermal capacity and reduced thermoresistance addressed the multiple limitations of PTT, and presented excellent in vitro and in vivo antitumor efficacy, having great potential for the clinical application of PTT.

Third order optical nonlinear properties of gold (Au) nanoparticles synthesized by green laser ablation in bio-based Poly(ε-caprolactone) solution

In this work, Poly(ε-caprolactone) (PCL) and gold(Au) nanocomposite has been fabricated by pulsed laser ablation (PLA) of gold target dipped in PCL solution performed by aid of Ti: Sapphire femtosecond (fs) laser to form nanoparticles (NPs). The prepared sample have been investigated by various analyses techniques to evaluate their elemental composition, optical and morphological properties. UV–Vis absorbance spectrum was recorded over a range of wavelength from 300 nm to 900 nm and it has shown a typical Au surface plasmon effect in visible region and PCL:Au nanocomposite absorbed more photons in UV region compared with that in infrared region. Photon energy band gap value has been calculated as 3.5 eV by means of absorbance spectrum. Transmission Electron Microscopy (TEM) and TEM-Energy dispersive X-Ray (TEM-EDX) images proved that the size of Au NPs appears to vary from 5 nm to 50 nm range produced using fs laser ablation in liquid medium. Fourier Transform Infrared Spectroscopy (FT-IR) analysis have been carried out for PCL and PCL:Au composite which they were characterized in over a frequency region from 400 to 4000 cm−1 and characteristic peaks were seen at 2905, 2805, 1755 and 1205 cm−1. Furthermore, nonlinear optical properties of PCL:Au composite was investigated using fs laser z-scan technique changing laser intensity between 9.50 × 1011 W/cm2 and 2.37 × 1012 W/cm2. The nonlinear absorption coefficient (β), nonlinear refractive indexes (n2) have been computed to be ∼10−10 - 10−11 cm2/W and ∼10−16 - 10−19 cm/W, respectively. Third-order susceptibilities have also revealed for PCL:Au nanocomposite as 10−13–10−14 esu. The obtained green synthesized PCL:Au composite material can be applicable in the optoelectronic field as optical limiting materials.

Acetone Vapor Sensors Based on Tin Dioxide Doped by Au Nanoparticles

The effect of nano-sized gold particles on the adsorption-sensitive properties of SnO2–Au sensors under the detection of acetone vapors has been studied. Different techniques for the preparation of SnO2–Au nanocomposites with an average Au particle size of 2 nm were applied. It has been found that a fivefold increase in the sensor response to acetone vapors and threshold sensitivity (Clim) of 0.1 ppm are achieved by adding gold to tin dioxide in the colloidal form during synthesis. While adding gold in ion form (Au (III)) leads to a growth of the sensor response to acetone vapors by 2.7 times and defines Clim of 0.2 ppm. The slope of the calibration curves of the SnO2–Au sensors allows registering acetone vapors at concentrations ranging from Clim to 5 ppm. This concentration range can be used for the express diagnostics in diabetes. The enhanced sensitivity of SnO2–Au sensors to acetone vapors can be explained by an increase in the adsorption-catalytic activity of tin ions as a result of the modifying effect of sulfate groups and the envolving of highly dispersed gold in the adsorption – catalytic process of oxidation of acetone molecules.

Improved Performance for the Electrochemical Sensing of Acyclovir by Using the rGO-TiO2-Au Nanocomposite-Modified Electrode.

An electrochemical sensor for sensitive sensing of acyclovir (ACV) was designed by using the reduced graphene oxide–TiO2–Au nanocomposite-modified glassy carbon electrode (rGO–TiO2–Au/GCE). Transmission electron microscopy, X-ray diffractometer, and X-ray photoelectron spectroscopy were used to confirm morphology, structure, and composition properties of the rGO–TiO2–Au nanocomposites. Cyclic voltammetry and linear sweep voltammetry were used to demonstrate the analytical performance of the rGO–TiO2–Au/GCE for ACV. As a result, rGO–TiO2–Au/GCE exerted the best response for the oxidation of ACV under the pH of 6.0 PB solution, accumulation time of 80 s at open-circuit, and modifier amount of 7 µl. The oxidation peak currents of ACV increased linearly with its concentration in the range of 1–100 µM, and the detection limit was calculated to be 0.3 µM (S/N = 3). The determination of ACV concentrations in tablet samples also demonstrated satisfactory results.

Highly Sensitive Electrochemical Immunosensor Platforms for Dual Detection of SARS-CoV-2 Antigen and Antibody based on Gold Nanoparticle Functionalized Graphene Oxide Nanocomposites.

In this work, we report a facile synthesis of graphene oxide–gold (GO–Au) nanocomposites by electrodeposition. The fabricated electrochemical immunosensors are utilized for the dual detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antigen and SARS-CoV-2 antibody. The GO–Au nanocomposites has been characterized by UV–vis spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy (EIS) for its biosensing properties. The linear detection range of the SARS-CoV-2 antigen immunosensor is 10.0 ag mL–1 to 50.0 ng mL–1, whereas that for the antibody immunosensor ranges from 1.0 fg mL–1 to 1.0 ng mL–1. The calculated limit of detection (LOD) of the SARS-CoV-2 antigen immunosensor is 3.99 ag mL–1, and that for SARS-CoV-2 antibody immunosensor is 1.0 fg mL–1 with high sensitivity. The validation of the immunosensor has also been carried out on patient serum and patient swab samples from COVID-19 patients. The results suggest successful utilization of the immunosensors with a very low detection limit enabling its use in clinical samples. Further work is needed for the standardization of the results and translation in screen-printed electrodes for use in portable commercial applications.

Formulation of a novel anti-human oral squamous cell carcinoma supplement by gold nanoparticles-Kaolin nanocomposite

In this work, we have described the biogenic synthesized gold nanoparticles being supported over Kaolin mediated by Pomegranate peel extract as natural reducing and stabilizing agent under mild conditions without any toxic reagents. Pomegranate peel extract was used as a green reducing agent and an excellent stabilizer of the synthesized Au NPs. Physicochemical characterization of the as-synthesized Kaolin@extract/Au NPs nanocomposite was carried out through electron microscopy (SEM and TEM), energy dispersive X-ray spectroscopy (EDX), elemental mapping, X-ray diffraction (XRD) and inductively coupled plasma-optical emission spectroscopy (ICP-OES). In the cellular and molecular part of the recent study, the treated cells with Au NPs-Kaolin nanocomposite were assessed by MTT assay for 48 h about the cytotoxicity and anti-human oral squamous cell carcinoma properties on normal (HUVEC) and oral squamous cell carcinoma cell lines i.e. HSC-2, HSC-3, HSC-4 and Ca9-22. It has been observed that the Au NPs with spherical shape and size were dispersed homogeneously on the modified-Kaolin surface. The significance of this study is the novel initiative to combine the gold nanoparticle with inexpensive Kaolin clay and utilization of them as anticancer agent. The IC50 of Kaolin@extract/Au nanocomposite were 277, 367, 250 and 545 µg/mL against HSC-2, HSC-3, HSC-4 and Ca9–22 cell lines, respectively. The viability of malignant human oral cell lines reduced dose-dependently in the presence of Kaolin@extract/Au NPs nanocomposite.

Au nanoparticles adorned chitosan-modified magnetic nanocomposite: An investigation towards its antioxidant and anti-hepatocarcinoma activity in vitro

• Supporting of Au NPs on chitosan-encapsulated magnetic Fe 3 O 4 nanoparticles. • The average diameter of the NPs was approximately 15–25 nm. • The cell viability of malignant liver cell line reduced dose-dependently in the presence of Fe 3 O 4 @CS-GA/Au nanocomposite. • Without significant toxicity against normal cell line. Chitosan is a linear polysaccharide and non-toxic bioactive polymer with a wide variety of applications due to its functional properties such as ease of modification and biodegradability. In this study, a green protocol for synthesis of Au nanoparticles (NPs) supported on chitosan-encapsulated magnetic Fe 3 O 4 NP is described. The morphological and physicochemical features of the material were determined using several advanced techniques like Fourier Transformed Infrared Spectroscopy (FT-IR), Field Emission Scanning Electron Microscopy (FE SEM), Transmission Electron Microscopy (TEM), Energy Dispersive X-ray Spectroscopy (EDS), X-ray Diffraction (XRD), Inductively Coupled Plasma (ICP), Vibrating sample Magnetometer (VSM) and elemental mapping. The average diameter of the NPs was approximately 15–25 nm. In addition, the Fe 3 O@CS-GA/Au nanocomposite was engaged in biological assays like study of anti-oxidant properties by DPPH mediated free radical scavenging test using BHT as a reference molecule. Thereafter, on having a significant IC 50 value in radical scavenging assay, we extended the bio-application of the desired nanocomposite in anticancer study of HepG2 liver cancer cell line in-vitro conditions following MTT assay. The cell viability of malignant liver cell line reduced dose-dependently in the presence of Fe 3 O 4 @CS-GA/Au nanocomposite. As a result, synthesized stable Fe 3 O@CS-GA/Au nanocomposite show more significant anticancer activity against HepG2 cells at 161.25 µg concentration of NPs without significant toxicity against normal cell line. The recent results suggest that Fe 3 O 4 @CS-GA/Au nanocomposite have a suitable anticancer activity against liver cell lines.