In this paper, by employing the asymptotic expansion method, we prove the existence and uniqueness of a smoothing solution for a time-dependent nonlinear singularly perturbed partial differential equation (PDE) with a small-scale parameter. As a by-product, we obtain an approximate smooth solution, constructed from a sequence of reduced stationary PDEs with vanished high-order derivative terms. We prove that the accuracy of the constructed approximate solution can be in any order of this small-scale parameter in the whole domain, except a negligible transition layer. Furthermore, based on a simpler link equation between this approximate solution and the source function, we propose an efficient algorithm, called the asymptotic expansion regularization (AER), for solving nonlinear inverse source problems governed by the original PDE. The convergence-rate results of AER are proven, and the a posteriori error estimation of AER is also studied under some a priori assumptions of source functions. Various numerical examples are provided to demonstrate the efficiency of our new approach.