1. Cultures of Aspergillus made a heavier, more rapid growth in a medium containing certain concentrations of manganese, copper, or zinc than in a medium free from these metals. 2. The optimum concentrations of these metals were low, and slightly larger quantities became toxic. 3. Combinations of the optimum concentrations of manganese, copper, and zinc stimulated a greater growth than did any one of the three metals, and all three produced a greater growth than did the combination of any two of these metals. 4. Manganese, copper, and zinc in the medium influenced slightly the assimilation of phosphorus, magnesium, and calcium. 5. The nitrogen content of Aspergillus grown in media containing manganese, copper, and zinc, or combinations of these elements, was less than in control cultures. 6. The fat content (ether extract) of Aspergillus was increased by manganese, copper, and zinc.
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