Various pharmacological activities of Clitoria ternatea (CT) have been reported in literatures such as anti‐oxidant and anti‐diabetic properties. The accumulation of advanced glycation end products (AGEs) in body tissue leads to several degenerations, atherosclerosis and diabetic complications. The objective of this study was to determine the inhibitory effect of CT extract on AGEs formation In Vitro using bovine serum albumin (BSA)‐fructose assay. The aqueous extract of CT was incubated with BSA and fructose at 37 □ for 14 days. The Amadori product (fructosamine) and amyloid cross β structure were determined by nitroblue‐tetrazolium and thioflavin T assay. The results showed that the fluorescent intensity of BSA incubated with fructose increased by 3.6 folds compared to BSA alone, indicating an evidently formation of AGEs. The CT extract (0.25–1.0 mg/ml) significantly inhibited formation of AGEs in a concentration‐dependent manner by approximately 24.11 ± 0.3% −55.97 ± 2.93 %. In addition, CT markedly reduced levels of fructosamine and amyloid cross β‐structure formation. Our results suggest that CT extract may exert anti‐glycation effect that could be offered as a compound for further study as a new natural product for prevention of AGE‐mediated diabetic complications.