Abstract
The construction of an enzyme electrode for one-step determination of fructose has been successful. The enzyme, D-fructose dehydrogenase (FDH), was immobilized on a membrane. Hexacyanoferrate (III) was used as a redox mediator and the change of current due to the presence of fructose was measured amperometrically. The permeability of Fe(CN) 6 −4 through different membranes was studied. The electrode response was linear in the range from 1·0 × 10 −5 to 1·0 × 10 −3 m of fructose with a correlation coefficient of 0·999. The relative standard deviation of the electrode was 0·68%. The developed electrode is stable for several weeks and assay of fructose with the electrode is not influenced by the presence of glucose or other sugars commonly found in food samples. Various conditions for fructose measurement with this assay system were examined and fructose contents in some food samples were determined in comparison with that of the AOAC standard method.
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