Aspergillus Sydowii Research Articles

Exploring the potential of katsuobushi grounds as a source of bioactive peptides through fermentation with Aspergillus sydowii

Dried bonito broth, made from katsuobushi flakes, is a commonly used seasoning in Japanese cuisine. While extracting the broth, high-quality proteins are left behind in the katsuobushi grounds, which could be a valuable source of bioactive substances via a hydrolysis process. The xerophilic Aspergillus sydowii showed potential for protein hydrolysis and production of amino acids and soluble peptides through solid-state fermentation. Several peptidases involved in katsuobushi protein hydrolysis were purified from the crude enzyme preparations and identified as aminopeptidase, trypsin-like serine protease, and elastinolytic serine protease. The hydrolysates from the culture contained glutamic acid, leucine, and lysine and markedly increased soluble peptides with molecular weights ranging from 0.2 to 17 kDa. Electronic tongue analysis indicated that the hydrolysates had substantially stronger salty and umami tastes. Moreover, the hydrolysates exhibited radical-scavenging activities, ferric ion reducing antioxidant power, and angiotensin converting enzyme inhibitory activity, as well as promoting growth in lactic acid bacteria. In addition, de novo peptide sequencing was performed for peptides fractionated from the hydrolysates, and eight peptides were identified. The hydrolysates produced by A. sydowii fermentation could have potential applications in the food industry as natural flavor enhancers and functional ingredients with several bioactivities.

Molecular Detection of Aspergillus Species from Food Sold in Amassoma, Nigeria

Fungal infections are quite common in humans and range from common, superficial infections to invasive and life-threatening infections. With at least 12 phyla and several fungal species globally, more than 500 species are expected to be infectious to man. One such is the ubiquitous Aspergillus species that mainly causes infection in immunocompromised individuals and those with underlying pulmonary disease. This study compared traditional identification methods and molecular biology techniques to isolate Aspergillus species from food samples sold in the University community. Some selected food samples frequently purchased in the community were analysed for the presence of Aspergillus species using both traditional and molecular biology techniques. Amplification of the ITS region of the rRNA genes of the isolate was performed using the ITS gene. A specie identified as Aspergillus niger was phenotypically identified as Aspergillus sydowii, and another species identified as Aspergillus flavus phenotypically was identified as Aspergillus fumigatus genotypically. The results demonstrate that traditional methods might be misleading, especially to a non-taxonomist. Genotypic identification proved to be more reliable and less time-consuming than phenotypic identification

Aflatoxin biosynthetic pathway extrolites in airborneAspergilli seriesVersicolores

TheAspergilli of the seriesVersicolores include several airborne species to which we are exposed daily. Most of them synthesise sterigmatocystin, a mycotoxin precursor of aflatoxins recognised as potentially carcinogenic for humans (group 2B) by the International Agency for Research on Cancer. Our objective was to study the potential role of these moulds in the synthesis of extrolites of the aflatoxin biosynthetic pathway. A total of 124 isolates belonging to the eight species of the seriesVersicolores collected in French bioaerosols were studied:Aspergillus amoenus (n=1),Aspergillus creber (n=45),Aspergillus fructus (n=2),Aspergillus jensenii (n=39),Aspergillus protuberus (n=6),Aspergillus puulaauensis (n=5),Aspergillus sydowii (n=14) andAspergillus tabacinus (n=2). All these isolates and a reference strain of each species were extracted using ethyl acetate acidified with 1% (v/v) acetic acid. In each extract, we screened for the following extrolites of the aflatoxin biosynthetic pathway by UPLC-HRMS: norsolorinic acid, versicolorin A, 6-demethylsterigmatocystin, sterigmatocystin, 8-O-methylsterigmatocystin, 5-methoxysterigmatocystin and aflatoxins B1, B2, G1 and G2. All extrolites, except aflatoxins, were found variably among species. Sterigmatocystin was found in extracts of all eightVersicolores species, including A.sydowii andA. tabacinus whose ability to synthesise sterigmatocystin was questioned or unknown, respectively.

Screening early markers of mildew upon cigar tobacco leaves by gas chromatography–ion mobility spectrometry (GC–IMS) and partial least squares–discriminant analysis (PLS–DA)

This study isolated two dominant molds (Penicillium chrysogenum and Aspergillus sydowii) from cigar tobacco leaves. Gas chromatography–ion mobility spectrometry (GC–IMS) was used for rapid qualitative and semi-quantitative analysis of mold growing from 0 to 7 days on potato dextrose agar (PDA). GC–IMS fingerprint, galleryplot, one-way analysis of variance (ANOVA), and partial least squares – discriminant analysis (PLS-DA) were used to assess different periods of cigar tobacco mildew. ANOVA and PLS-DA were compared to identify possible mildew markers in PDA. The potential mildew markers were verified using 0–7 days of artificial mildew samples. The results showed that 1-octene-3-alcohol, 1-pentanol, and pentanal were characteristic markers of early mildew in cigar tobacco. In conclusion, GC–IMS and chemometrics enables early warning of cigar tobacco mold, which is of great significance for storage and quality control.

Fungal Catastrophe of a Specimen Room: Just One Week is Enough to Eradicate Traces of Thousands of Animals.

Indoor fungi obtain carbon sources from natural sources and even recalcitrant biodegradable materials, such as plastics and synthetic dye. Their vigorous activity may have negative consequences, such as structural damage to building materials or the destruction of precious cultural materials. The animal specimen room of the Seoul National University stocked 36,000 animal resources that had been well-maintained for over 80years. Due to abandonment without the management of temperature and humidity during the rainy summer season, many stuffed animal specimens had been heavily colonized by fungi. To investigate the fungal species responsible for the destruction of the historical specimens, we isolated fungi from the stuffed animal specimens and identified them at the species level based on morphology and molecular analysis of the β-tubulin (BenA) gene. A total of 365 strains were isolated and identified as 26 species in Aspergillus (10 spp.), Penicillium (14 spp.), and Talaromyces (2 spp.). Penicillium brocae and Aspergillus sydowii were isolated from most sections of the animal specimens and have damaged the feathers and beaks of valuable specimens. Our findings indicate that within a week of mismanagement, it takes only a few fungal species to wipe out the decades of history of animal diversity. The important lesson here is to prevent this catastrophe from occurring again through a continued interest, not to put all previous efforts to waste.

Production, characterization and techno-economic evaluation of Aspergillus fusant l-asparaginase

Protoplast fusion is one of the most reliable methods of introducing desirable traits into industrially-promising fungal strains. It harnesses the entire genomic repertoire of fusing microorganisms by routing the natural barrier and genetic incompatibility between them. In the present study, the axenic culture of a thermo-halotolerant strain of Aspergillus candidus (Asp-C) produced an anti-leukemic l-asparaginase (l-ASNase) while a xylan-degrading strain of Aspergillus sydowii (Asp-S) produced the acrylamide-reduction type. Protoplast fusion of the wild strains generated Fusant-06 with improved anti-leukemic and acrylamide reduction potentials. Submerged fed-batch fermentation was preferred to batch and continuous modes on the basis of impressive techno-economics. Fusant-06 l-ASNase was purified by PEG/Na+ citrate aqueous two-phase system (ATPS) to 146.21-fold and global sensitivity analysis report revealed polymer molecular weight and citrate concentration as major determinants of yield and purification factor, respectively. The enzyme was characterized by molecular weight, amino acid profile, activity and stability to chemical agents. Michaelis–Menten kinetics, evaluated under optimum conditions gave Km, Vmax, Kcat, and Kcat/Km as 6.67 × 10–5 M, 1666.67 µmolmin−1 mg−1 protein, 3.88 × 104 min−1 and 5.81 × 108 M−1.min−1 respectively. In-vitro cytotoxicity of HL-60 cell lines by Fusant-06 l-ASNase improved significantly from their respective wild strains. Stability of Fusant-06 l-ASNase over a wide range of pH, temperature and NaCl concentration, coupled with its micromolar Km value, confers commercial and therapeutic value on the product. Free-radical scavenging and acrylamide reduction activities were intermediate and the conferred thermo-halo-stability could be exploited for sustainable clinical and food industry applications.

Fungicidal Activity of Silver and Silica Nanoparticles against Aspergillus sydowii Isolated from the Soil in Western Saudi Arabia

Aspergillus sydowii is a mesophilic soil saprobe that is a food contaminant as well as a human pathogen in immune-compromised patients. The biological fabrication of silica and silver nanoparticles provides advancements over the chemical approach, as it is eco-friendly and cost-effective. In the present study, Aspergillus sydowii isolates were collected from the soil fields of six different sites in the western area of Saudi Arabia and then identified using the PCR technique following sequencing analysis by BLAST and phylogenetic analysis. Then, applied silica and silver nanoparticles were synthesized by biological methods, using Aspergillus niger as a reducer. Silver and silica nanoparticles were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). The antifungal activity of silver and silica nanoparticles against Aspergillus sydowii isolates was evaluated using the disc diffusion method and the minimum inhibitory concentration (MIC). The physiochemical results emphasized the fabrication of silver and silica nanoparticles in spherical shapes with a diameter in the range of 15 and 40 nm, respectively, without any aggregation. MIC of Ag-NPs and Si-NPs against Aspergillus sydowii isolates were 31.25 and 62.5 µg/mL, respectively. Finally, the aim of the study is the use of silver as well as silica nanoparticles as antifungal agents against Aspergillus sydowii.

Study on inoculation fermentation by fungi to improve the taste quality of summer green tea

Summer green tea (SGT) is not favored by consumers due to its strong bitterness and astringency. This study aims to improve the taste of SGT using tea-derived fungi isolated from Liupao tea by solid-state fermentation. Based on 19 detected main compounds, principal component analysis (PCA) and hierarchical cluster analysis (HCA) showed Aspergillus sydowii, Penicillium manginii, Aspergillus intermedius, Aspergillus amstelodami, and Aspergillus niger groups were clustered together with significant differences from the control. Additionally, metabolomics analysis showed P. manginii, A. intermedius, and A. amstelodami strains could reduce the chemical components associated with bitter and astringent flavors, such as epicatechin gallate, epicatechin, (+)-gallocatechin, quercetin, kaempferol, myricetin, procyanidin B2, L-phenylalanine and its glycoside derivatives (VIP >1, P < 0.05), and increase the contents of theabrownins associated with umami taste (P < 0.05). In summary, the taste of SGT can be improved by the above fungi fermentation.

Characterization of erythromycin-degrading strain Aspergillus sydowii W1 revealed by transcriptome analyses

Erythromycin residual, as a persistent, antibiotic pollutant, poses a serious threat to environmental and human health. Thus, developing effective technologies to eliminate erythromycin from the environment is urgent. In this study, a new fungus W1 identified as Aspergillus sydowii which can utilize erythromycin as its only carbon source was isolated from soil contaminated with erythromycin. The degradation efficiency of erythromycin by strain W1 was 84.05% within seven days under optimal conditions (30 °C, pH 6.0 and 100 mg L−1 erythromycin). The degradation pathway of erythromycin was proposed from four intermediates detected by HPLC-MS, and enzymes involved in erythromycin metabolism in strain W1 were preliminarily inferred. Notably, genomic and transcriptomic analysis also confirmed that strain W1 degrades erythromycin via glycoside hydrolase and esterase activity. This study demonstrated that A. sydowii W1 is a novel erythromycin-degrading strain, which has great potential for removing erythromycin from contaminated soils, and also provides a new perspective for other applications of this particular strain.

Phenol derivatives from the cold-seep fungus Aspergillus sydowii 10–31

Two bisabolane-type sesquiterpenoid phenols inclusive of the known (7 S )-flavilane A ( 1 ) and the new (7 S )− 4-iodo-flavilane A ( 2 ), along with a new tetraphenyl ether, bisviolaceol II ( 3 ), were isolated from the cold-seep fungus Aspergillus sydowii 10–31. Their structures were identified through spectroscopic methods, such as MS, IR, and NMR, and the absolute configurations of 1 and 2 were ascertained by comparison of ECD curves. Compounds 1 and 2 represent rarely encountered sulfur-bearing sesquiterpenes in nature, and the iodination in 2 makes it unique in particular. Additionally, 3 is the first tetraphenyl ether derivative from the fungal species A. sydowii . Compounds 1 - 3 inhibited the growth of several Vibrio bacteria and harmful microalgae. • Two new metabolites from a cold-seep isolate of Aspergillus sydowii are reported. • (7 S )− 4-Iodo-flavilane A is the first natural sulfur- and iodine-containing sesquiterpene. • Biviolaceol II represents the first tetraphenyl ether derivative from Aspergillus sydowii .

BIO-MANAGEMENT OF FUNGAL LEAF SPOT OF TOMATO (SOLANUM LYCOPERSICUM L.) USING INDIGENOUS TRICHODERMA ISOLATES

One of the main obstacles to feeding the world is the fungal leaf spot disease, which has a negative impact on plants' photosynthetic areas and significantly lowers crop quality and output. To manage fungi that pose a serious hazard to both humans and the environment, various chemical fungicides are utilized. The goal of the current study was to determine whether local isolates of three Trichoderma spp. (Trichoderma viride, Trichoderma harzianum, and Trichoderma asperellum) had any antagonistic effects in vitro against the pathogenic fungi Alternaria alternata, Fusarium solani, Fusarium oxysporum, Aspergillus flavus, Aspergillus sydowii, and Alternaria sp. Trichoderma viride showed the greatest growth inhibition against all of the tested pathogenic fungi, followed by Trichoderma harzianum and Trichoderma asperellum. It was shown that all three Trichoderma species strongly inhibited the mycelial growth of fungal pathogens. Compared to other isolated fungi, Trichoderma species inhibited Alternaria alternata mycelial growth more, whereas in Fusarium oxysporum least amount of mycelial growth inhibition was observed. These findings imply that Trichoderma species can function as an effective biocontrol agent against the fungi responsible for tomato leaf spot disease.

Interspecific protoplast fusion of atmospheric and room-temperature plasma mutants of Aspergillus generates an L-asparaginase hyper-producing hybrid with techno-economic benefits

The axenic culture of Aspergillus candidus (Asp-C) produced an anti-leukemic L-asparaginase while Aspergillus sydowii (Asp-S) produced the acrylamide-reduction type. Upon mutagenesis by atmospheric and room-temperature plasma (ARTP), their individual L-asparaginase activities improved 2.3-folds in each of Ile-Thr-Asp-C-180-K and Val-Asp-S-180-E stable mutants. Protoplast fusion of selected stable mutants generated fusant-09 with improved anti-leukemic activity, acrylamide reduction, higher temperature optimum and superior kinetic parameters. Submerged (SmF) and solid-state fermentation (SSF) types were compared; likewise batch, fed-batch and continuous fermentation modes; and fed-batch submerged fermentation was selected on the basis of impressive techno-economics. Fusant L-asparaginase was purified by PEG/Na+ citrate aqueous two-phase system and molecular exclusion chromatography to 69.96 and 146.21-fold, respectively, and characterized by molecular weight, specificity, activity and stability to chemical and physical agents. Michaelis–Menten kinetics, evaluated under optimum conditions gave K m, V max, K cat, and K cat/K m as 1.667 × 10−3 M, 1666.67 µmol min−1 mg−1 protein, 645.99 s−1 and 3.88 × 105 M−1 s−1 respectively. In-vitro cytotoxicity of HL-60 cell lines by fusant-09 L-asparaginase improved 3.00 and 18.71-folds from mutants Ile-Thr-Asp-C-180-K and Val-Asp-S-180-E, and from 5.73 and 32.55 from respective original strains. Free-radical scavenging and acrylamide reduction improvements were intermediate. Fusant-09 L-asparaginase is strongly recommended for sustainable economic anti-leukemic and food industry applications.

Isolation and Screening of potent cellulolytic soil fungi from Raipur city of Chhattisgarh State, India

The current research focuses on the diversity of fungal isolates obtained from the soil and their ability to produce cellulolytic enzymes. Out of 76 colonies obtained, 11 fungal colonies were isolated viz. Aspergillus , Humicola and Rhizopus being the major genera of the isolates. From the 11 isolates, the identification of potent cellulase producing cultures was done using qualitative carboxy methyl cellulase screening test. Following the preliminary screening, five cultures viz., Aspergillus sydowii , Humicola sp - 1, Aspergillus niger , Aspergillus ustus , Aspergillus flavus were identified to be potent with enzymatic indices ranging from 1.25 to 2.29. These were further selected for quantitative enzyme analysis. Aspergillus sydowii was found to have highest enzymatic activity and Aspergillus flavus was found to have the least enzymatic activity. Our findings revealed that Aspergillus sydowii appeared to be a promising candidate for cellulase production and can be utilized for waste recycling and other biotechnological applications.

Black gill disease in Litopenaeus vannamei made by various agents

Melanization of shrimp gills is a general symptom in shrimp black bill diseases (sBG); therefore, the cause is not immediately apparent. This study aimed to determine the primary and secondary agents associated with a black gill outbreak in the Delvar complex in the Bushehr Province of Iran. During July and August of 2020, 450 healthy and affected shrimps were collected based on brown to black gill color from three farms of the Bushehr Province in Iran. Characterization of pathogens was performed by gross signs, wet mounts, microbiological tests, histopathology methods, and sequencing of molecular markers. The mean weight and length of infected shrimps was significantly (P < 0.05) lower than the healthy shrimps. The histological examination of sBG indicated the adhesion of secondary lamellae, accumulation of hemocyte cells at the end of the secondary lamellae, and necrosis of epithelial cells with pyknosis and karyorrhexis. According to our results, Aspergillus sydowii as a primary agent correlates with sBG disease in Litopenaeus vannamei from Iran, and other biotic factors, including bacterial (Vibrio owensii and V. parahaemolyticus) probably are secondary agents associated with sBG disease.

Research Advances of Bioactive Sesquiterpenoids Isolated from Marine-Derived Aspergillus sp.

Marine fungi Aspergillus sp. is an important source of natural active lead compounds with biological and chemical diversity, of which sesquiterpenoids are an extremely important class of bioactive secondary metabolites. In this paper, we review the sources, chemical structures, bioactivity, biosynthesis, and druggability evaluation of sesquiterpenoids discovered from marine fungi Aspergillus sp. since 2008. The Aspergillus species involved include mainly Aspergillus fumigatus, Aspergillus versicolor, Aspergillus flavus, Aspergillus ustus, Aspergillus sydowii, and so on, which originate from sponges, marine sediments, algae, mangroves, and corals. In recent years, 268 sesquiterpenoids were isolated from secondary metabolites of marine Aspergillus sp., 131 of which displayed bioactivities such as antitumor, antimicrobial, anti-inflammatory, and enzyme inhibitory activity. Furthermore, the main types of active sesquiterpenoids are bisabolanes, followed by drimanes, nitrobenzoyl, etc. Therefore, these novel sesquiterpenoids will provide a large number of potential lead compounds for the development of marine drugs.

S5.3b Fungal spores: Initiators of colonization and infection

S5.3 Cellular pleomorphism and fungal virulence, September 22, 2022, 3:00 PM - 4:30 PMFungi produce asexual and sexual spores for reproduction and distribution, which can be both in space and time. Distribution in space occurs, by air movement, but also, by water or other vectors such as living organisms. Filamentous fungi from the division Ascomycota that belong to the order Eurotiales produce asexual spores called conidia. Conidia are moderately stress-tolerant cells and are able to survive unfavorable conditions such as thermal stress, dehydration, osmotic pressure, oxidative stress, variations in pH, and UV. For example, conidia of the fungus Penicillium chrysogenum are isolated worldwide and must be regarded as cosmopolitan. In many cases, conidia might ‘land’ closely to the location of production, but still many spores making into the higher air layers. There is indirect evidence that spores may be able to travel large distances through the air. For example, Aspergillus sydowii conidia have been suggested to travel over thousands of kilometers from the Sahara Desert to the Caribbean reefs. Distribution in time is occurring as stress-resistant cells remain dormant at one location for an extended period, awaiting conditions that are more favorable for growth. Some ascospores (sexual spores) are extremely stress-resistant and dormant for very long periods. Other species show extended dormancy in a dried state. As microbial species are inherently variable, stress resistance varies between strains from the same species. For example, conidial heat resistance (D60) of various strains of the fungus Paecilomyces variotii ranged between 3.5 to 27.6 min. This intraspecific variation could have profound consequences on diagnostics, virulence, and antifungal treatment in clinical settings. For conidial germination in most filamentous fungi, the presence of nutrients such as inorganic salts, sugars, and amino acids is required. The swelling phase of conidia is also called isotropic growth. Swollen conidia direct the growth to one side of the cell to grow in a polarized fashion, which leads to the formation of a germ tube (polarized growth). There is a notable drop in stress resistance during isotropic and polarized growth and genes expressed during these stages might represent novel targets for fungal infection.

Bioprospecting endophytic fungi for antifeedants and larvicides and their enhancement by gamma irradiation

The search and discovery of new natural products with antifeedant and larvicidal potentials to mitigate harmful insects are scientific pressing issues in the modern agriculture. In this paper, the antifeedant and larvicidal potentials of 69 fungal isolates were screened against the Egyptian cotton leafworm Spodoptera littoralis. A total of 17 isolates showed the insecticidal potentials with three promising isolates. These strains were Aspergillus sydowii, Lasiodiplodia theobromae, and Aspergillus flavus isolated from Ricinus communis (bark), Terminalia arjuna (Bark), and Psidium guajava (twigs), respectively. The effect of gamma irradiation on the antifeedant and larvicidal activities of the three strains was investigated. Exposure of the fungal spores to 1000 Gy of gamma rays significantly intensified both the antifeedant and larvicidal potentials. To identify compounds responsible for these activities, extracts of the three strains were fractionated by thin layer chromatography. The nature of the separated compounds namely, Penitrem A, 1, 3, 5, 8- tetramethyl- 4, 6-diethyl- 7- [2- (methoxycarbonyl)ethyl] porphyrin (from A. sydowii), Penitrem A, 2, 7, 12, 17-Tetramethyl-3, 5:8, 10:13, 15:18, 20-tetrakis (2,2-dimethylpropano) porphyrin (from A. flavus), N,N-Diethyl-3-nitrobenzamide, and Diisooctyl-phthalate (from L. theobromae) were studied by GC-MS analysis. These findings recommend endophytic fungi as promising sources of novel natural compounds to mitigate harmful insects.

Two sydowic acid derivatives and a sulfonyl metabolite from the endophytic fungus Aspergillus sydowii

Two new sydowic acid derivatives, a pair of enantiomers, involving (+)-sydowiccal (1a) and (−)-sydowiccal (1b), a new sulfonyl metabolite of 2-methoxy-5-methyl-3-(methylsulfonyl)phenol (2), as well as three known sydowic acid derivatives, were isolated from Aspergillus sydowii, an endophytic fungus of Rhododendron mole. The structures of these new compounds were elucidated by analyzing their NMR and HRESIMS data, and the absolute configurations of enantiomers were determined on the basis of the CD spectrum. Three new metabolites showed weak anti-inflammation on nitric oxide (NO) production in LPS-induced RAW 264.7 cells.

Phenotypic and molecular diagnosis using polymerase chain reaction (PCR) technology for some aflatoxin-producing fungi isolated from food

The study was conducted in the laboratories of the College of Education for Pure Sciences for the academic year 2021-2022 on fungi that produce aflatoxin toxin that were isolated from some foodstuffs traded in the local markets in the city of Karbala and were diagnosed molecularly using a technique Polymerase chain reaction (PCR) which successfully doubled NS8 and NS1 with prefixes (SSU-) The same primers were used to determine the sequence of nitrogenous bases of rRNA belonging to the International Center for Information Technology Genbank. National Center of Biotechnology Information (NCBI) it has been deposited in a database (fungi biogenic). The results of isolation, which were collected from imported and local foodstuffs, were contaminated with fungi, and that all isolated fungi were producing aflatoxin toxin. This was inferred by using coconut medium with ammonia solution. hf ifheehehai eifenhihehidnhf eht dna Penicillium oxalicum, Penicillium expansum, Aspergillus flavus, Cladosporium Uredinicola, Aspergillus sydowii, Aspergillus Oryzae, Aspergillus Tamarii, Aspergillus nomius, Alternaria triticina, Penicillium griseofulvum, Aspergillus austwicki, Aspergillus flavus, Cladosporium cladosporioides, Actinomucor elegans, Penicillium brevicompatum, Trichophyton mentagrophytes, Gibberella intermedia, Debaryomyces hansenii, Aspergillus caespitosus, Aspergillus versicolor.

Genome analysis and CAZy repertoire of a novel fungus Aspergillus sydowii C6d with lignocellulolytic ability isolated from camel rumen

BackgroundCamels are known for their survival under harsh and nutrient-deficient climates. Camel rumen ecosystem presents a unique opportunity to study the ruminal microbes helping the camel in this task. The genus Aspergillus is the extensively studied filamentous fungus due to its ability to secret industrially important enzymes. The present study was aimed to isolate and characterize microbes with lignocellulolytic capacity from camel rumen. ResultsThe fungal isolate Aspergillus sydowii C6d, isolated from camel rumen, was sequenced and analysed for its CAZyme content responsible for lignocellulose degradation. The C6d isolate was evaluated for its capacity to produce cellulase, pectinase, xylanase, and amylase with their respective assays and further evaluated for their optimum pH. The genome sequencing and assembly resulted in 32.27 Mb of genome size with a GC % of 50.59. The CAZyme analysis revealed that the C6d produced 543 polysaccharide-degrading CAZymes amongst which, 148 CAZymes were potentially involved in lignocellulose degradation. The genomic comparison of the C6d with 30 commonly used lignocellulolytic fungi (white rot, brown rot, and soft rot fungus) showed the enrichment of cellulolytic and pectinolytic CAZymes in C6d genome as compared to others. The saccharification of lignocellulosic substrate wheat straw resulted in the release of 50.85% of reducing sugars. ConclusionsThe study provides important insights into the CAZymes responsible for lignocellulolytic ability in the novel fungus Aspergillus sydowii C6d isolated from camel rumen and presents a valuable source of CAZymes to be further evaluated for potential biotechnological applications.How to cite: Tulsani NJ, Jakhesara SJ, Hinsu AT, et al. Genome analysis and CAZy repertoire of a novel fungus Aspergillus sydowii C6d with lignocellulolytic ability isolated from camel rumen. Electron J Biotechnol 2022;59. https://doi.org/10.1016/j.ejbt.2022.06.004.