Aspartate Aminotranspherase Research Articles

Effect of Antihistamines (Fexofenadineand Chlopheneramine) on activity some hepatical enzymes by using fertilized hens eggs embryos as a model

The aim of the present study was to investigate the effect of antihistamines(Chlorpheneramineand Fexofenadine) on (alkaline phosphatase, aspartate amino transpherase and alanine amino transferase) in tissue of fertilized hens eggs embryos as indicator to tissue damage. The injection of fertilized hen's eggs with antihistamines Fexofenadine at 12th day of incubation in doses (0,5,0,1mg/ egginto air sell)after 24 hour of treatment induce significantly decrease in tissues activity of alkaline phosphatase (ALP),aspartate amino transpherase (AST) and alanine amino transferase (ALT) compared with control group in adose-dependent manner.Ingection of Chlorpheneramineat dose (0,5,0,1 mg/egginto air cell)induce significantly decrease in tissues activity ofalkaline phosphatase and aspartate amino transpherase compared with control group, but no significant decrease in tissue activity of alanine amino transpharase(ALT)at dose(0,5,0,1mg/egg) compared with control group.Compare the effect of ChlorpheneramineandFexofenadine on some biochemical parameters, chlorpheneramine cause significan decrease intissues activity of alkaline phosphatase (ALP) and alanine amino transferase (ALT) larger than the group which terated withfexofenadine in the same dose, also chlorpheneramine cause significan decrease intissues activity of aspartate amino transferase larger than the group which treated with fexofenadine in same dose.We conclode the antihistamines hasharmful effects to tissueschick embryorepresented by the changes in activity of some biochemical parameter which its consider as a marker for damage this tissue

The Differences of T-Regulator Cells, Alanine Aminotransferase Serum and Aspartate Aminotranspherase Between Hepatitis B Chronic Patients with and without Liver Fibrosis

Background: Hepatitis B is acute or chronic liver inflammation caused by hepatitis B viral and can progress to hepatic chirrosis or liver cancer. Chronic hepatitis B has a high risk for liver fibrosis. Chronic inflammation and liver fibrosis are interrelated processes. This study aimed to determine the differences in T-regulator cells, Alanine Aminotransferase (ALT) and Aspartate Aminotransferase (AST) between chronic hepatitis B patients with and without liver fibrosis.Method: This study used a cross-sectional method for patients diagnosed with chronic hepatitis B in the Inpatient and Outpatient Department of the Internal Medicine Department DR. M. Djamil Padang and other hospitals in Padang city for 6 months. Samples were selected by consecutive sampling according to inclusion and exclusion criteria. Liver fibrosis is identified by fibroscan. Data were analyzed by SPSS 21.0.Results: thirty-two patients were diagnosed with chronic hepatitis B and 50% had liver fibrosis. The levels of T-regulator cells in chronic hepatitis B patients without liver fibrosis were 2.08% and liver fibrosis 2.25%, but this difference was not statistically significant (p 0.05). Mean ALT levels in the group without fibrosis were 19 IU/L (7IU/L-71IU/L) and liver fibrosis 61 IU / L (13IU/L-625IU/L). The mean AST level in the group without fibrosis were 15.5 IU/L (10IU/L-32IU/L) and liver fibrosis 35.5 IU/L (10IU/L-476IU/L). The difference between ALT and AST in the two groups was significant (p 0.05). Hepatitis B patients with liver fibrosis had higher ALT and AST levels than without fibrosis.Conclusion: There were differences levels of T-regulator cells in the two groups, but it was not statistically significant. ALT and AST levels were higher in the liver fibrosis group and statistically significant.

Evaluation of the effects of hair colouring products on the oxidative status in rats

IntroductionPermanent hair dyes, oxidant creams, and henna are usually used for cosmetic purposes and sometimes for therapeutic expectations. The effects of these products, which are used to change hair colour and can be absorbed percutaneously on the oxidative status is not known exactly.AimTo investigate the effects of these products, which have various contents, on the oxidative status using an in vivo rat model.Material and methodsThe products used for hair colouring were prepared as recommended for human use and applied to the back region of Wistar albino rats. Superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) levels were measured in serum and liver samples of rats. The Kruskal-Wallis test showed significant differences in serum SOD, aspartate aminotranspherase (AST), alanine aminotranspherase (ALT), and liver MDA levels among the study groups.ResultsThere were statistically significant positive correlations between hepatic MDA values and AST and ALT values. Hair dyes, oxidant creams, and henna were found to have oxidative and hepatotoxic effects. Surprisingly, comparisons revealed that oxidative effect and hepatic toxicity of the oxidant cream and henna were similar. The oxidant cream was more oxidating and hepatotoxic than the hair dye.ConclusionsKnowing the facts about these products, which are easily accessible to every individual in society and are considered to be innocent, will prevent possible harm.

Prometheus therapy for the treatment of acute liver failure in patients after cardiac surgery.

IntroductionAcute liver failure usually develops in multiple organ dysfunction syndrome and significantly increases the mortality risk in patients after cardiac surgery.AimTo assess the safety and efficacy of extracorporeal liver support in patients with acute liver failure after cardiac surgery.Material and methodsWe studied 39 adult patients with multiple organ dysfunction syndrome and acute liver failure as postoperative complication, treated with Prometheus therapy. Inclusion criteria comprised clinical and laboratory signs of acute liver failure. Criteria to start Prometheus therapies were: serum bilirubin above 180 µmol/l (reference values: 3–17 µmol/l), hepatocyte cytolysis syndrome (at least 2-fold increase in aspartate aminotranspherase and alanine aminotranspherase concentrations; reference values 10–40 U/l) and decrease in plasma cholinesterase (reference values 4490–13 320 U/l).ResultsExtracorporeal therapy provided stabilization of hemodynamics, decrease in serum total bilirubin and unconjugated bilirubin levels, decrease in cytolysis syndrome severity and positive effect on the synthetic function of the liver. The 28-day survival rate in the group treated with Prometheus therapy was 23%.ConclusionsPrometheus procedures could be recommended as a part of combined intensive care in patients with acute liver failure after cardiac and major vessel surgery. The efficiency of this method could be improved by a multi-factor evaluation of patient condition in order to determine indications for its use.

Development of a Summarized Health Index (SHI) for use in predicting survival in sea turtles.

Veterinary care plays an influential role in sea turtle rehabilitation, especially in endangered species. Physiological characteristics, hematological and plasma biochemistry profiles, are useful references for clinical management in animals, especially when animals are during the convalescence period. In this study, these factors associated with sea turtle surviving were analyzed. The blood samples were collected when sea turtles remained alive, and then animals were followed up for surviving status. The results indicated that significantly negative correlation was found between buoyancy disorders (BD) and sea turtle surviving (p < 0.05). Furthermore, non-surviving sea turtles had significantly higher levels of aspartate aminotranspherase (AST), creatinine kinase (CK), creatinine and uric acid (UA) than surviving sea turtles (all p < 0.05). After further analysis by multiple logistic regression model, only factors of BD, creatinine and UA were included in the equation for calculating summarized health index (SHI) for each individual. Through evaluation by receiver operating characteristic (ROC) curve, the result indicated that the area under curve was 0.920 ± 0.037, and a cut-off SHI value of 2.5244 showed 80.0% sensitivity and 86.7% specificity in predicting survival. Therefore, the developed SHI could be a useful index to evaluate health status of sea turtles and to improve veterinary care at rehabilitation facilities.

The role of dietary L-ascorbyl-2-polyphosphate on the growth and physiological functions of beluga,Huso huso(Linnaeus, 1758)

Effects of dietary ascorbic acid (AA) supplementation were carried out on growth performance and physiological indices in juvenile beluga Huso huso fed different levels of dietary L-ascorbyl-2-polyphosphate as AA source consisting of 0 (control), 100, 200, 400, 800 and 1600 mg AA kg−1 diet for 16 weeks. No significant differences were found in growth performances between treated groups except for condition factor. Using AA caused significant differences in haemoglobin, number of red blood cells, white blood cells, aspartate aminotranspherase and lactate dehydrogenase, while no significant difference was found in haematocrit, mean corpuscular haemoglobin concentration, differential white blood cells count and alkaline phosphatase activity at the end of the experiment. Lysozyme activity after 8 weeks was significantly affected by dietary treatments with the lowest value in fish fed control diet, but not at completion of 16-week feeding trial. Liver AA concentration showed a significant increase in correlation with dietary AA increase. Air exposure stress test showed significant differences among the treatments in case of cortisol and glucose concentrations. Also between pre- and post stress, significant changes were observed in some of treatments. The lowest and highest levels of cortisol were 9.2 ± 3 (in 200 mg kg−1) and 16.8 ± 5.1 ng mL−1 (in control), 6 h post stress (P < 0.05) respectively. Also, the lowest and highest levels of glucose were measured in fish fed 200 and 800 mg AA kg−1 respectively. The obtained results showed that most growth parameters were not affected by dietary AA, but some physiological and immunological parameters of this species were influenced by this vitamin. We suggest that under rearing conditions, AA should be added to the diet especially during early life stages for improving health status and resistance to stressors.

Dişi Köpeklerde Ovaryohisterektominin Vücut Ağırlığı, Total Kolesterol, Trigliserit, Karaciğer Enzim Değerleri ve Glikoz Seviyesi Üzerine Erken Dönem Etkilerinin Araştırılması

There have been many studies which investigated the effect of ovariohysterectomy on metabolic activity in bitches. The slowing down effect of ovariohysterectomy on metabolism and it’s results was emphasized by the researchers. The purpose of the presented study is to determine the early term effects of neutering on bodyweight, serum total cholesterol, trigliceride, alkaline phosphatase, alanin aminotranspherase, aspartate aminotranspherase and glucose values in bitches with different breed, age and living conditions. For this purpose serum total cholesterol, triglyceride, glucose levels and body weight of 15 bitches were determined at pre-operative and post-operative 3rd th and 12th months. When the body weights of different times were considered statistically, the difference between pre and postoperative 6th and pre and post-operative 12th month bodyweights were significant (P<0.05). The results of serum parameters were non-significant statistically. As a result of this study, ovariohysterectomy in bitches have no significant early-time effect on serum total cholesterol, trigliceride, alanine aminotranspherase, alkaline phosphatase, aspartate aminotranspherase and glucose level whereas body weight gain occurs after post-operative 6th month but it can be managed by proper nutrition and exercise programmes.

The activity of some enzymes in the frog's liver (Rana ridibunda Pall.) in the different natural conditions of thermal water regime

During the period of March–July 2011, the activity of aspartate aminotranspherase (AST), alanine aminotranspherase (ALT), lactate dehidrogenase (LDH) and alkaline phosphatase (ALP) enzymes was measured in the liver of the Rana ridibunda Pall. natural frog population.Altogether 52 frogs were collected for the research purposes in the two rivers of a different water temperature regime respectively, the thermal water spring of Banja of Malishevë (24.7°C – 29.0°C) and river Tërdec (7.9°C – 19.7°C).There were not recorded great oscillations of AST and ALT activity in the liver of frogs from the thermal water, and ALT activity from the river Tërdec as well during the period of investigation. At the same time in the liver of frogs from the river Tërdec, the lower activity of AST has been recorded in March (12.7±2.6 ), whereas a higher activity in May (41.4±8.4 ), and in July (46.6± 7.6). The distinctive characteristic of this research is that only in March, the activity of AST in the frogs liver from the river Tërdec is significantly at lower level compared with values of frogs from the thermal water.Despite AST, in the liver of the frogs of two localities there were not recorded large oscillations of the ALT, LDH and ALP activity values during the entire period of research.According to the research results we could conclude that the water temperature (the cold stress), the seasonal changes of the environmental temperature, the nutrition, and the metabolic activity of the frogs, affects the activity of AST.

Seasonal changes in the hemolymph biochemistry of the snail Helix aspersa

The seasonal rhythm of the annual concentration of glucose, total proteins, albumins, cholesterol, triglycerides and the activity of aspartate aminotranspherase (AST) and the alanin aminotranspherase (ALT) enzymes in the hemolymph of the snail Helix aspersa (N=180) was researched. The results of the research show seasonal oscillations of the aforementioned parameters in the hemolymph. The highest concentration of the researched biochemical parameters was recorded in the winter, with the significant changes in autumn‐winter (glucose, P&lt;0.001), and summer‐winter (total proteins, albumins, cholesterol and triglycerides (P&lt;0.01). The increased activity of AST and ALT transaminases has been recorded during the summer and early autumn, whereas their lower activity has been recorded in the winter and early spring.The higher concentration of biochemical parameters in the hemolymph during the winter is probably connected with their crioprotector role, whereas the increased activity of the AST and ALT enzymes in the summer could probably be connected to the nutrition and the metabolic level.

BLOOD BIOCHEMISTRY REFERENCE VALUES FOR WILD JUVENILE LOGGERHEAD SEA TURTLES (CARETTA CARETTA) FROM MADEIRA ARCHIPELAGO

Standard biochemical parameters were determined in wild juvenile loggerhead sea turtles Caretta caretta living offshore Madeira Island, northeast Atlantic. We analyzed the influence of age, sex, sea surface temperature, and body condition index on biochemical parameters including uric acid, total bilirubin, total cholesterol, creatinine kinase (CK), glucose, total protein, urea nitrogen, lactate dehydrogenase, aspartate aminotranspherase (AST), gamma-glutamyl transferase (GGT), albumin, alkaline phosphatase (ALP), sodium (NA), potassium (K), chloride, calcium, phosphorus, and magnesium. Significant positive correlations were found between turtle body size and total cholesterol, total protein, and albumin. Total protein and the enzymes AST and CK were lower than reported levels in adults. Calcium levels were lower than those reported in adult or captive turtles, but similar to wild juveniles from Australian waters, and were interpreted as normal for this age category. These data may be useful to evaluate the health status of stranded or injured animals and to improve veterinary care at rehabilitation centers.

Effect of water acidification on performance, carcass characteristic and some variables of intermediary metabolism in chicks

The effect of water acidification on performance and some biochemical parameters was studied in 100 one day old broiler chicks (Ross 308) divided into control and treatment groups (n = 50) supplied with drinking water supplemented with acidifier (blend of acids) in a concentration of 0.2 % during the whole experimental period. Final body weight, hot carcass yield, abdominal fat pad and metabolic variables in the blood serum except for aspartate aminotranspherase (AST) level which was significantly lower on 35th day (p<0.01), were not affected by the acidifier. Higher weight gain in the treated group due to better feed efficiency was observed in the first phase. Higher feed intake in the third phase of the trial caused higher final feed conversion ratio (p<0.01). Use of the acidifier positively affected bird health status (zero mortality) which was reflected in higher EEI values observed in the treated group on the 35th, as well as 42nd day of the trial.

PERFIL BIOQUÍMICO DE CABRAS LACTANTES DAS RAÇAS SAANEN E ANGLO-NUBIANA

BIOCHEMICAL PROFILE OF SAANEN AND ANGLO-NUBIAN LACTATING GOATS Although nutrient requirements are now relatively well-defined for sheep and cattle, there is still a lack of reliable information in the literature about the nutritional needs of lactating goats. Most of the information available are based on extrapolated values obtained in cattle and sheep experiments. Nevertheless, due to specific physiological features of the goat, this approach requires further validation. Thus, to determine the biochemical profile of Saanen and Anglo-Nubian lactating goats, a total of 20 serum samples were collected and submitted to laboratorial analysis. The aim was to determine serum concentrations of total protein, aspartate aminotranspherase, gama-glutamyltranspherase, alkaline phosphatase, urea, creatinine, calcium, phosphorus, magnesium, sodium, potassium, and ionized calcium. Our results reveal the influence of genotype on some of the biochemical components in both breeds of lactating goats.

PERFIL BIOQUÍMICO SÉRICO DE CABRAS DAS RAÇAS SAANEN E BOER

BIOCHEMICAL SERUM PROFILE OF SAANEN AND BOER GOATS It is crucial to determine an animal's serum biochemical profile in order to evaluate its nutritional state, making it possible to detect metabolic alterations as well as assist clinical diagnosis of several diseases. To be successful in such a task, it is essential to consider the reference values for different species according to breed, sex, and age. Thus, a total of 20 serum samples from Saanen and Boer goats were collected and submitted to laboratorial analysis for the determination of serum concentrations of total protein, aspartate aminotranspherase, gama-glutamyltranspherase, alkaline phosphatase, urea, creatinine, calcium, phosphorus, magnesium, sodium, potassium, and ionized calcium. The results revealed that breed type had no influence on the assessment of biochemical profiles.

The first case of variant Creutzfeldt-Jakob disease in the Netherlands

The first case of variant Creutzfeldt-Jakob disease in the Netherlands

The Study on the Relationship of Abomasal Displacement and Fatty Liver Syndrome in Dairy Cows

Background: In cattle, diseases of abomasum are of great importance and include right and left displacement, torsion, impaction, pyloric stenosis and ulceration. Displacement of the abomasum has become one of the most important metabolic and organic internal disorders of cattle and the disease is most prevalent in high performing milk breeds. Abomasal displacement occurs most frequently in high yielding cows during early lactation. It has been reported that feeding a large amount of concentrations or corn silage to dairy cows inhibits the motility of the resulting in gas accumulation followed by dilation and atony and thereby causing bovine displaced. Abomasal displacement, ketosis, parturient paresis, retain placenta and endometritis has been often associated with a fatty liver. Aims: This investigation was undertaken to determine the relationship of abomasal displacement and fatty liver syndrome in dairy cows. Methods: this study, twelve cows with abomasal displacement (7 RDA, 5 LDA) and 10 healthy cows (after calving) have been used as materials. Age of animals varied from 3 to 7 years. Blood and liver biopsy samples were taken from all the cows. Sera were analysed for glucose, cholesterol, triglyceride, Total bilirubine, calcium, magnesium, phosphorus, Aspartate aminotranspherase (AST), Alkalen phosphates (ALP), Creatin kinase (CK), total protein (TP) and albumin (Alb) . Results: In 10 of all the cases, various degree fat infiltration of liver was observed. But two cases have not shown any fat infiltration of the liver. The differences in AST, glucose and Mg concentrations of healthy cows compared to cows with abomasal displacement were found to be significant (p<0.01). The differences in ALT, Ca and albumin concentrations of healthy cows compared to cows with abomasal displacement were found to be significant (p<0.05). But, there was no significant in ALP, cholesterol, triglyceride, total bilirubine, Ca, P and TP concentrations of healthy cows compared to cows with abomasal displacement. Conclusion: The results of the present study that showed various degree fat infiltration of the liver in the cows with abomasal displacement.

Cardiac Enzymes after Transmyocardial Laser Treatment with CO2 Laser

Objectives - The aim of the present study was to examine postoperative serum levels of cardiac enzymes after transmyocardial laser treatment (TML) and to evaluate any associations between this release, postoperative cardiac events and change in ejection fraction after 3 months' follow-up. Design - Forty-nine patients with angina pectoris Canadian Cardiovascular Society Angina Score Class III & IV refractory to medical therapy and untreatable by coronary artery bypass or percutaneous transluminal angioplasty treated with CO2 laser were included. Inclusion criteria were age less than 75 years, left ventricular ejection fraction greater than or equal to 30% and myocardial regions with reversible ischemia. Serum levels of aspartate aminotranspherase (ASAT), alanine aminotranspherase (ALAT) and MB-isoenzymes of creatine kinase (CK-MB) were followed during the first 72 h after surgery. Ejection fractions were estimated by multiple-gated acquisition ventriculography at inclusion and 3 months postoperatively. Results - A significant increase in serum markers of myocardial necrosis was observed 8 h after surgery. A subsequent increase from 8 to 24 h after surgery was associated with the presence of postoperative cardiac adverse events. An inverse correlation was found between peak level of cardiac enzymes and change in ejection fraction from baseline to 3 months' follow-up. Conclusions - TML with CO2 laser is followed by a significant increase in serum levels of cardiac enzymes after 8 h. Further significant increases are associated with cardiac adverse events postoperatively. Peak enzyme values are inversely correlated with change in ejection fraction from baseline to 3 months' follow-up.

Isozyme Variability in Plum (Prunus Domestica), and its use for Cultivar and Interspecific Hybrid Identification

ABSTRACTThe polymorphism of seven enzyme systems in plum (Prunus domestica) was analyzed by vertical polyacrilamide gel electrophoresis in two different somatic tissues, bark and leaves. Variation among the studied plum cultivars was observed in Peroxidase. Interspecific hybrids between plum and apricot exhibited Aspartate aminotranspherase pattern with bands from both parents. This enzyme system my be used for plum x apricot interspecific hybrid identification.