The activity of some enzymes in the frog's liver (Rana ridibunda Pall.) in the different natural conditions of thermal water regime

Abstract

During the period of March–July 2011, the activity of aspartate aminotranspherase (AST), alanine aminotranspherase (ALT), lactate dehidrogenase (LDH) and alkaline phosphatase (ALP) enzymes was measured in the liver of the Rana ridibunda Pall. natural frog population.Altogether 52 frogs were collected for the research purposes in the two rivers of a different water temperature regime respectively, the thermal water spring of Banja of Malishevë (24.7°C – 29.0°C) and river Tërdec (7.9°C – 19.7°C).There were not recorded great oscillations of AST and ALT activity in the liver of frogs from the thermal water, and ALT activity from the river Tërdec as well during the period of investigation. At the same time in the liver of frogs from the river Tërdec, the lower activity of AST has been recorded in March (12.7±2.6 ), whereas a higher activity in May (41.4±8.4 ), and in July (46.6± 7.6). The distinctive characteristic of this research is that only in March, the activity of AST in the frogs liver from the river Tërdec is significantly at lower level compared with values of frogs from the thermal water.Despite AST, in the liver of the frogs of two localities there were not recorded large oscillations of the ALT, LDH and ALP activity values during the entire period of research.According to the research results we could conclude that the water temperature (the cold stress), the seasonal changes of the environmental temperature, the nutrition, and the metabolic activity of the frogs, affects the activity of AST.