Asparagus Racemosus Root Extract Research Articles

Mitochondrial resilience and antioxidant defence against HIV-1: unveiling the power of Asparagus racemosus extracts and Shatavarin IV.

Asparagus racemosus (AR), an Ayurvedic botanical, possesses various biological characteristics, yet its impact on HIV-1 replication remains to be elucidated. This study aimed to investigate the inhibitory effects of AR root extracts and its principal bioactive molecule, Shatavarin IV (Shatavarin), on HIV-1 replication and their role in mitigating mitochondrial dysfunction during HIV-1 infection, utilizing both in vitro and in silico methodologies. The cytotoxicity of the extracts was evaluated using MTT and ATPlite assays. In vitro anti-HIV-1 activity was assessed in TZM-bl cells against X4 and R5 subtypes, and confirmed in peripheral blood mononuclear cells using HIV-1 p24 antigen capture ELISA and viral copy number assessment. Mechanistic insights were obtained through enzymatic assays targeting HIV-1 Integrase, Protease and Reverse Transcriptase. Shatavarin's activity was also validated via viral copy number and p24 antigen capture assays, along with molecular interaction studies against key HIV-1 replication enzymes. HIV-1 induced mitochondrial dysfunction was evaluated by detecting mitochondrial reactive oxygen species (ROS), calcium accumulation, mitochondrial potential, and caspase activity within the infected cells. Non-cytotoxic concentrations of both aqueous and hydroalcoholic extracts derived from Asparagus racemosus roots displayed dose-dependent inhibition of HIV-1 replication. Notably, the hydroalcoholic extract exhibited superior Reverse Transcriptase activity, complemented by moderate activity observed in the Protease assay. Molecular interaction studies revealed that Shatavarin IV, the key bioactive constituent of AR, formed hydrogen bonds within the active binding pocket site residues crucial for HIV replication enzyme catalysis, suggesting its potential in attenuating HIV-1 infection. Mitochondrial dysfunction induced by HIV-1 infection, marked by increased oxidative stress, mitochondrial calcium overload, loss of mitochondrial membrane potential, and elevated caspase activity, was effectively mitigated by treatment with AR extracts and Shatavarin IV. These findings underscore the potential of AR extracts and Shatavarin IV as antiviral agents, while enhancing mitochondrial function during HIV-1 infection. In conclusion, Asparagus racemosus extracts, particularly Shatavarin IV, demonstrate promising inhibitory effects against HIV-1 replication while concurrently ameliorating mitochondrial dysfunction induced by the virus. These findings suggest the therapeutic potential of AR extracts and Shatavarin in combating HIV-1 infection and improving mitochondrial health.

Biosynthesis of Copper Nanoparticles with an Ethanolic Asparagus racemosus Root Extract: Environmental Protection Against Blue-Green Algae and its Bactericidal Potential

This study was done to prove the environmental application of copper nanoparticles, particularly theantimicrobial activity against bacterial pathogens, Pseudomonas aeruginosa and Staphylococcus aureus. The root extract ofAsparagus racemosus was used to synthesize copper nanoparticles (Cu-NPs) via biosynthesis. Cu-NPs were characterizedusing a UV-Visible spectrophotometer, X-ray diffraction, Fourier transform infrared spectroscopy, and scanning electronmicroscopy. The antibacterial activity of Cu-NPs was studied by treating nanoparticles with two bacterial pathogens,Pseudomonas aeruginosa and Staphylococcus aureus. The biosynthesized copper nanoparticles displayed satisfactoryantibacterial activity against bacterial pathogens. Antioxidant and antidiabetic assays were performed via the enzymeinhibition property. The biosynthesized Cu-NPs exhibited a satisfactory antifouling function to eradicate algae growth.

Apoptotic effect of reduced Graphene Oxide on MCF-7 Breast cancer cell line using Asparagus Racemosus roots

In the modern era, the major health issue is cancer. Cancer is one of the second- deadliest death-causing diseases worldwide. Graphene is a carbon-based material that has drawn a great deal of attention because of its eccentric chemical and physical properties. Moreover, graphene can be transformed into graphene oxide or reduced graphene oxide, depending on its application. Chemical reduction of graphene oxide was associated with toxic and harmful reducing compounds; hence, the green, efficient and cost-effective approach for graphene synthesis is using plant extract. In this contemporary study, the reduced graphene oxide can be synthesized from Asparagus racemosus root extract, involving anti-cancer activity. The reduced graphene oxide was characterized from UV-Visible, FT-IR, SEM and XRD. The size and shape of the synthesized reduced graphene oxide were confirmed from SEM studies. The anti-cancer activity showed a strong cytotoxic effect on the tested cell line.

Green Synthesis of TiO2 Nanoparticles Using Root Extract of Asparagus racemosus (Shatavari) and Evaluation of its Antibacterial, Antioxidant and Antidiabetic activities

Since ancient times, Asparagus racemosus, a traditional herb with various health advantages, has been used to cure a wide range of illnesses. Researchers sparked a lot of interest in the biological methods for producing nanoparticles (NPs). This study’s objective was to synthesize titanium dioxide nanoparticles (TiO2 NPs) utilizing the top-down nanofabrication method. Asparagus racemosus root extract was used to produce titanium nanoparticles utilizing green chemistry. The nanoparticles were characterized using UV-Visible spectroscopy, SEM (Scanning Electron Microscope), and EDX. According to the SEM images the particles are spherical in shape with an average size of 89.59 nm. The antibacterial activity was done with 3 bacterial strains i.e., E. coli, Pseudomonas aeruginosa and Bacillus subtills. Antioxidant activity was evaluated by DPPH and phosphomolybdate assays, the calculated IC50 was 85.18±0.55 µg/ml and 66.4±1.3 µg/ml respectively. The calculated IC50 value for alpha amylase assay was 46.59±2.81 µg/ml. As per the outcomes it is concluded that the plant mediated titanium nanoparticle is very potent which can be used in pharmaceutical and biomedical areas.

Antibacterial activity and cytotoxic effect of bisphosphonate conjugated gold nanoparticle synthesized using asparagus racemosus root extract

Nanoparticles are being widely used in the field of medicine called Nanomedicine. Biological membranes in the human body are selectively permeable to Nanoparticles. The nanoparticles can be surface modified to attach a variety of ligands which enable them to be biosensors, molecular-scale fluorescent tags, imaging agents, and targeted drug delivery vehicles. Green synthesis of nanoparticles is a type of bottom-up approach in which reaction occurs with the help of reducing and stabilizing agents. Bisphosphonates are increasingly being used to treat a wide range of skeletal issues, including hereditary skeletal disorders in children, cancer-related bone metastases and osteoporosis. Asparagus racemosus is a green edible medical plant used in Ayurvedic preparations to treat aging, vigor, immunity, longevity, and skeletal issues. It has been shown to have a variety of pharmacological properties, including antioxidant, antimicrobial, anti-inflammatory, and antiseptic properties. Bisphosphonate conjugated with gold nanoparticle have shown to produce more targeted action.The goal of this study was to green synthesise Bisphosphonate conjugated gold nanoparticles with asparagus racemosus extract and to check their antimicrobial activity and cytotoxicity. Green synthesis of Bisphosphonate conjugated gold nanoparticles using Asparagus racemosus extract was achieved and is characterized.

Asparagus racemosus mediated silver chloride nanoparticles induce apoptosis in glioblastoma stem cells in vitro and inhibit Ehrlich ascites carcinoma cells growth in vivo

Glioblastoma multiforme (GBM) is a type of brain tumor that is most aggressive, proliferates rapidly and intensive invasion is governed by cell migration and destruction of the extracellular matrix. In the present study, we evaluated the antiproliferative efficacy of the synthesized silver chloride nanoparticles (AgCl-NPs) from Asparagus racemosus root extract against human glioblastoma stem cells (GSCs) and Ehrlich ascites carcinoma (EAC) cells. Biosynthesis of A. racemosus- AgCl-NPs was confirmed by color change, UV–visible spectroscopy and characterized by transmitted electron microscope, energy dispersive spectroscopy, x-ray powder diffraction and Fourier-transform infrared spectroscopy. The A. racemosus- AgCl-NPs inhibited GSCs and EAC cells growth with the IC 50 values of 4.8 and 4.69 µg/ml, respectively. A. racemosus- AgCl-NPs induced apoptosis in GSCs which was confirmed by annexin V/PI assay, various genes expression, and caspase-3 protein expression as detected by the immunofluorometric assay. The expression level of the TLR9, NFκB, TNFα, p21 and IKK genes were increased consequently with the decrease of PARP, EGFR, NOTCH2, mTOR and STAT3 genes in GSCs as examined by real-time PCR. The cell cycle arrest at G 0 /G 1 phase was detected by flow cytometry. In addition, A. racemosus- AgCl-NPs caused significant inhibition of EAC cells growth, reduced tumor burden, increased the survival of EAC-bearing mice and restored the hematological parameters when compared with the control mice. The synthesized AgCl-NPs inhibited the proliferation of GSCs in vitro with the induction of apoptosis and inhibited the growth of EAC cells in vivo in mice by reducing the tumor burden and increasing the survival periods.

Green synthesis and characterization of bisphosphonate conjugated gold nanoparticle with Asparagus racemosus root extract.

Bisphosphonates improve orthodontic anchorage. More targeted action of this drug can be achieved through its conjugation with gold nanoparticles. Asparagus racemosus is a green edible medicinal plant used in Ayurvedic preparations to treat aging, vigor, immunity, longevity, and skeletal issues. Therefore, it is of interest to report the green synthesized Bisphosphonate conjugated gold nanoparticles with Asparagus racemosus extract and to characterize them.

Bactericidal action of copper nanoparticles synthesized from methanolic root extract of Asparagus racemosus

The main aim of the study is to exhibit an antibacterial activity using copper nanoparticles against two bacterial pathogens such as Escherichia coli, and Staphylococcus aureus. Initially, Copper nanoparticles were synthesized by using the Asparagus racemosus root extract and the centrifuged copper nanoparticles were dried in normal room temperature for the future use. Then Ultra Violet analysis, X-ray Diffraction method, Fourier Transform Infrared Spectroscopy and Zeta potential were used to characterize for the confirmation of copper nanoparticles. The antibacterial activities were experimentally studied using two pathogens (Escherichia coli, and Staphylococcus aureus) and this result shows a very good antibacterial activity in biosynthesized copper nanoparticles.

Evaluation of Solvent Extraction Process for Asparagus racemosus Root Extract through the Determination of its Phenolic Content and Antioxidant Activity Assay

This study is aimed to evaluate extraction capacity of different solvents through determining total phenolic content, total flavonoid content, total saponin content, and an antioxidant assay of different solvent extracts (toluene, dichloromethane, chloroform, ethyl acetate, ethanol, methanol, water, 60% aqueous methanol, 60% aqueous ethanol and 60% aqueous acetone) of Asparagus racemosus (A. racemosus) root. The 60% aqueous methanol shows the highest extracting yield, in contrast, toluene shows the lowest yield. Highest total phenolic content (220.8±0.74mg GAE/g), total flavonoid content (219.3±0.64mg QCE/g), total tannin content (108.4±0.88mg GAE/g) were found from acetone extract. The methanol extract showed the highest saponin content (579.4±2.85mg ESE/g). Antioxidant potential determines by using various in vitro methodologies such as DPPH, FRAP, and ABTS assay. The lowest IC50 value was found in acetone extract and thus it exhibited the highest antioxidant activity. The highest correlation was found between phenolic content and the antioxidant assay. It seems that phenolic contents are responsible for free radicle scavenging activity. From the observation, it concluded that acetone extract rich with polyphenolic content and methanol extract shows the highest amount of saponin content.

Antibacterial and Immunomodulatory Potentials of Biosynthesized Ag, Au, Ag-Au Bimetallic Alloy Nanoparticles Using the Asparagus racemosus Root Extract.

Two noble metals, such as silver and gold alloy nanoparticles, were successfully synthesized by the microwave assisted method in the presence of the Asparagus racemosus root extract and were used as an antibacterial and immunomodulatory agent. The nanostuctures of the synthesized nanoparticles were confirmed by various spectroscopic and microscopic techniques. The UV-vis spectrum exhibits a distinct absorption peak at 483 nm for the bimetallic alloy nanoparticles. The microscopic analysis revealed the spherical shaped morphology of the biosynthesized nanoparticles with a particle size of 10–50 nm. The antibacterial potential of the green synthesized single metal (AgNPs and AuNPs) and bimetallic alloy nanoparticles was tested against five bacterial strains. The bimetallic alloy nanoparticles displayed the highest zone of inhibition against P. aeurgnosia and S.aureus strains when compared to single metal nanoparticles and plant extract. In addition, the inmmunomodulatory potential of the root extract of A. racemosus, AgNPs, AuNPs, and Ag-Au alloy NPs is achieved by measuring the cytokine levels in macrophages (IL-1β, IL-6, and TNF-α) and NK cells (IFN-γ) of NK92 and THP1 cells using the solid phase sandwich ELISA technique. The results showed that the root extract of A. racemosus, AgNPs, and AuNPs can reduce the pro-inflammatory cytokine levels in the macrophages cells, while Ag-Au alloy NPs can reduce cytokine responses in NK92 cells. Overall, this study shows that the microwave assisted biogenic synthesized bimetallic nanoalloy nanoparticles could be further explored for the development of antibacterial and anti-inflammatory therapies.

Asparagus racemosus Root Extract and Isoprinosine Exhibits Radio-mitigating Activity against Ionizing Radiation-induced Detrimental Effects in Swiss Albino Mice

Objectives: To evaluate the radio – mitigating property of Asparagus racemosus root ethanolic extract and Isoprinosine against ionizing radiationinduced harmful effects in Swiss albino mice. Methods: The experimental animals were exposed to sublethal dose (6 Gy) of whole-body electron beam radiation and administered orally with Asparagus racemosus root ethanolic extract (ARE – 200mg/kg bd. wt) and Isoprinosine (IPR – 400 mg/ kg bd. wt) for 15 consecutive days. After the experimental period, the animals were sacrificed. Blood and liver tissue were collected. Blood sample was used for assessment of hematological parameters, serum was used for biochemical analysis (lipid peroxidation, total antioxidant capacity) and the level of pro-inflammatory cytokines was determined in the liver homogenate. Results: The radiation control group showed a statistically significant reduction in the hematological parameters. IPR post-treatment group showed a substantial increase in the percentage of lymphocytes and monocytes. ARE and IPR post-treatment caused a statistically significant improvement in platelet count. Irradiation of mice resulted in a significant elevation in lipid peroxidation. Post-treatment of mice with ARE and IPR caused significant depletion in lipid peroxidation. The serum total antioxidant capacity (TAC) in the irradiated group decreased significantly. Administration of ARE to the animals after irradiation caused a significant elevation in TAC level. IPR post-treatment did not show any substantial change in the reduced level of TAC. The cytokines level in the liver homogenate of irradiated mice showed that Interleukin -2 (IL-2), Tumor Necrosis Factor alpha (TNF-α) and Interferon gamma (IFN-γ) significantly increased after radiation exposure. Administration of ARE and IPR after irradiation significantly reduced the levels of IL-2 and TNF-α in the posttreatment groups. An elevated level of IFN-γ was reduced considerably in the IPR post-treatment animals. Conclusion: Asparagus racemosus root ethanolic extract and Isoprinosine exhibit radio-mitigating effects against electron beam radiation - induced detrimental effects. This occurs through several mechanisms, such as free radical scavenging, anti-inflammation, facilitation of repair activity, regeneration of hematopoietic cells and affecting to molecular levels. Key words: Asparagus racemosus, Isoprinosine, Radio-mitigation, Ionizing radiation.

Biogenic synthesis of stable silver nanoparticles via Asparagus racemosus root extract and their antibacterial efficacy towards human and fish bacterial pathogens

Herein, we demonstrate the synthesis of silver nanoparticles via a greener approach using Asparagus racemosus root extract and stability and antimicrobial activity of fabricated Ag NPs were evaluated. The Ag NPs were synthesized using the AgNO3 source with Asparagus racemosus root extract as a capping and reducing agent. The prepared Ag NPs were characterized using UV–visible, SEM-EDX, XRD, TEM, FTIR techniques. The antibacterial activity of Ag NPs was examined against Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Klebsiella pneumonia, Pseudomonas fluorescence, Aeromonas hydrophila, Edwardsiella tarda, Flavobacterium branchiophilum, and Yersinia rukeri. The resultant antibacterial activity of green AgNPs is a hope and an assurance to nano drug formulations to tackle bacterial infections against human as well as fish bacterial pathogens.

Chemometric analysis of selective polyphenolic groups in Asparagus racemosus (Shatavar) root extracts by traditional and supercritical fluid (CO2) based extractions

ABSTRACTAsparagus racemosus root extracts were prepared by supercritical fluid (CO2), soxhlet, and maceration-based methods also with various pretreatments. Thereafter, these root extracts were analyzed by High-Performance Liquid Chromatography, along with the chemometric study of the disparate phenolic groups. Among these, supercritical fluid (CO2) based extract has a larger number of polar compounds and the antioxidant activity (98.54 ± 0.22 µM Trolox equivalent mg−1). It also has the best cell viability (94.37 ± 1.12%) and insulin release (0.82 ± 1.12 ng mL−1) on β-pancreatic RINm-5F cells whereas, the best extractive yield (75.80 ± 3.44% w/w) was observed for pretreated aqueous soxhlet-based extract.

Anticancer activity of Asparagus racemosus root extracts in non-small cell lung cancer A549 cells

Anticancer activity of Asparagus racemosus root extracts in non-small cell lung cancer A549 cells

Antioxidant Activities of Ethanolic and Aqueous Extracts of Asparagus racemosus Roots

Background: Asparagus racemosus (AR) is commonly known as shatavari, satawar or satmuli in India and in Thailand it is call sam-sib or rak-sam-sib. The dried root of AR is used in Ayurveda as an antiulcerous and antiinflammatory and has medicinal/pharmacological value. Objective: To investigate the antioxidant activities of Asparagus racemosus root extracts via total phenolic and total flavonoid contents of ethanolic and aqueous extracts. Methods: Antioxidant capacity measurements were carried out by DPPH, ABTS and FRAP methods. Total phenolic and flavonoid contents were determined by the Folin-Ciocalteu method and the aluminum chloride colorimetric method, respectively. Results: The ethanolic extract possessed higher antioxidant capacities than the aqueous extract in the three antioxidant assays (p<0.05).These results have shown high phenolic and flavonoid contents. The ethanolic extract of AR root possessed higher amounts of phenolic and flavonoid contents than the aqueous extract. Conclusion: The antioxidant capacity of the ethanolic extract was higher than that in the aqueous extract.

Nephroprotective efficacy of Asparagus racemosus root extract on acetaminophen-induced renal injury in rats

Acetaminophen-induced renal necrosis and insufficiency occurs in patients with acetaminophen overdose. Renal failure is rapidly assuming epidemic proportions globally. In absence of reliable and effective nephroprotective drugs, strategies towards exploring alternative therapies for treatment of kidney diseases are essential. Asparagus racemosus is a medicinal plant used for treatment of various ailments. This research was undertaken to investigate the protective effect of ethanol fraction of A. racemosus roots extract in acetaminophen-induced uraemia and renal failure in rats. Rats were co-administered with acetaminophen injection and oral administration of A. racemosus roots extract in an attempt of protection against renal failure. Uremic biomarkers significantly decreased, and elevated levels of antioxidant enzymes were found, in the animals treated with ethanol fraction of A. racemosus when compared with acetaminophen treated uremic animals. Also, histology of kidneys showed control like structure in animals treated with this extract but severe damage in the uremic animals. HPLC analysis of the ethanol fraction of A. racemosus roots extract revealed eight compounds out of which one had a retention time near to the quercetin standard. It may be concluded that this extract of A. racemosus has therapeutically useful nephroprotective potential.

Effects of Asparagus racemosus Root Extracts on Serum Lipid Profiles, Lipid Peroxidation and Superoxide Dismutase in Ovariectomized Rat

Background: Once rats have been ovariectomized they have a high risk of cardiovascular disease due to changes in the blood cholesterol and lipid profile. Objective: To investigate the effects of Asparagus racemosus (AR) root extract on the serum lipid profiles, lipid peroxidation and antioxidant levels in ovariectomized rats. Methods: Twenty-five, two month-old female Wistar rats were randomly divided into five groups: SH, OVX,OEE, OAAR and OEAR. The daily doses of 500 mg/KgBW of the AR root extracts for five weeks.The levels of serum TG, TC, HDL, LDL, the liver, kidney and uterine tissue lipid peroxidation and SOD levels were determined. Results: Serum TC and LDL showed no significant differences in any groups. Serum TG of the OAAR and OEAR groups were not significantly different. The serum HDL of the OAAR and OEAR groups were significantly lower than the OEE group. The liver MDA levels of the OAAR and OEAR groups were significantly decreased compared with the OVX and OEE groups while the SOD level of the OAAR group was significantly increased. The MDA levels in the kidney and uterine of the treated group showed no significant difference. The SOD levels in the kidney of the treated group were not different but the SOD levels in uterine were significantly decreased. Conclusion: It can be believed that the lipid profiles were maybe regulated via estrogen.The AR extract has low effects on the lipid profiles at this dose and duration of treatment. The capacity of the extracts to decrease the MDA level and increase the SOD level in this study clearly reflected the antioxidant efficiency of these substances.

Neuroprotective effect of Asparagus racemosus root extract via the enhancement of brain-derived neurotrophic factor and estrogen receptor in ovariectomized rats

Ethnopharmacological relevanceAsparagus racemosus (AR) is well known as an Ayurvedic rasayana which used traditionally by Ayurvedic practitioners for nervous disorders and prevent aging. In our previous study it was found that ethanol AR root extract can improve learning and memory impairment, induced by an ovariectomy, but the extract's mechanisms as a neuroprotective property are still unknown. Aim of the studyThis study aimed to examine the effects and mechanisms of ethanol AR root extract on the alteration of brain-derived neurotrophic factor (BDNF) and estrogen receptor (ER) subtypes in ovariectomized (OVX) rats. Materials and methodsAdult female Wistar rats were divided into five groups, 4 groups underwent ovariectomy, and one group was designed to be the sham control group. Two groups were gavaged with propylene glycol for sham, and a second group similarly prepared for OVX. Two further groups of OVX rats were gavaged once daily, one group with 100 mg/kg b.w. of ethanol AR root extract and the second group with 1000 mg/kg b.w. of ethanol AR root extract. The fifth group was gavaged once daily with 0.1 mg/kg b.w. of 17α-ethynylestradiol (EE). BDNF, ERα and ERβ expression were evaluated by western blot analysis. ResultsThe western blot analysis revealed that the OVX rats showed a significant decrease in BDNF and a down-regulation of ERα and ERβ in the frontal cortex and hippocampus. It was also demonstrated that EE and AR root extract increased BDNF, ERα and ERβ in the frontal cortex and hippocampus of ovariectomized rats. ConclusionsBased on these results, the enhancement of BDNF and ERs up-regulation may be involved in the neuroprotective effects of ethanol AR root extract in ovariectomized rat.

Protective Efficacy of Asparagus racemosus root extract and Isoprinosine against Ionizing Radiation - induced Clastogenicity and Toxicity in Swiss Albino Mice

: Objective: We aimed to evaluate anticlastogenic and radioprotective potential of Asparagus racemosus root extract (ARE) and Isoprinosine (IPR) against electron beam radiation (EBR) induced clastogenicity and toxicity in Swiss albino mice. Methodology: In the pre-radiation study, the experimental animals were orally administered ARE – 200mg and IPR – 400mg/ kg b.wt once daily for 15 consecutive days. The animals exposed to sublethal dose (6Gy) of whole body EBR. Chromosomal aberration analysis and micronucleus assay were carried out in the bone marrow cells of the experimental animals. The various types of aberrations were scored and the micronuclei in Polychromatic Erythrocytes (PCE) and Nomochromatic Erythrocytes (NCE) were recorded. Assessment of Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), was performed using mouse GM-CSF Picokine ELISA kit. Non-specific Alpha – esterase activity was determined by simultaneous azo dye coupling method. Dose Reduction Factor (DRF) was calculated to determine the protective role of ARE and IPR against EBR. Result: Treatment of mice with ARE-200 mg/kg b.wt and IPR-400mg/kg b.wt decreased the percentage of the total aberration compared to the irradiated group; significantly reduced (P<0.05) the frequency of Mn PCE and Mn NCE when compared with irradiation alone groups. Irradiation reduced the level of GM-CSF in the splenocytes which was enhanced by the pre-treatment with ARE and IPR. There was a significant increase in the number of alpha-esterase positive cells in the pre-treatment group compared to radiation control. Increase in survival percentage was observed in the pre-treated mice when compared to radiation alone group. The DRF value of 1.11 and 1.04 was observed respectively. Conclusion: The present study suggests that the antioxidant potential of ARE and IPR could be of extreme significance in offering radioprotection and may be useful in combating various free-radical and reactive oxygen species – mediated human pathological conditions.Key words: Asparagus racemosus root extract, Isoprinosine, Electron beam radiation, Clastogenicity, Toxicity.

Radioprotective potential of Asparagus racemosus root extract and isoprinosine against electron beam radiation-induced immunosupression and oxidative stress in swiss albino mice

Background: Radiotherapy is an important and the most common treatment modality for human cancers. Cancer radiotherapy is associated with unadorned side effects that results from normal tissue damage which is a major subject of concern. Radiation induces damage to living cells due generation of aqueous-free radicals. Therefore, there is a crucial need for the protection of normal cells surrounding the tumor from radiation injury; and hence, the identification of radiation-protecting agents is a chief goal for basic radiation biologists and oncologists. Aim: The aim of this present study was to assess the radioprotective potential of Asparagus racemosus root ethanolic extract (ARE), and isoprinosine (IPR) against electron beam radiation (EBR)-induced immunosuppression and oxidative stress in Swiss Albino mice. Materials and Methods: Swiss albino mice were used for the assessment of the radioprotective potential of ARE and IPR against EBR-induced immunosuppression and oxidative stress. Cytokine estimations, namely, interleukin-2, interferon-gamma, and tumor necrosis factor-alpha were performed in the liver homogenate using ELISA kits, and bone marrow cellularity was determined in the experimental animals. Results: The results of the present study demonstrated the radioprotective and immunostimulatory efficacy of ARE and IPR against EBR-induced cytokine and bone marrow cellularity alterations. Conclusion: The findings of our study demonstrate the potential of ARE and IPR in mitigating radiation-induced mortality by offering protection to mice against lethal dose of whole body EBR. The present study also demonstrates that ARE and IPR exert its radioprotection against EBR induced immunosuppression by regulating cytokines.