Artificial Animal Research Articles

Why Look at Toy Animals?

Abstract This article addresses the toy as a neglected cultural and technical object. The toy is neither tool nor ritual object, and its animation in children's imaginative play suggests alternative perspectives on the history and lived experience of material and technological artifacts. The concept of protopolitics is advanced to explore the implications for the cultural politics of the ambiguous articulation of power relationships in play. The article takes the long history of the toy animal as a case study, drawing attention to its creaturely, artificial facets that go beyond, or more accurately precede, familiar cultural-political binaries of authentic and inauthentic, depth and surface, knowledge and illusion, truth and lies, belief and fetishism, human and nonhuman, natural and synthetic. These other facets include dynamics of the technics of imagination, and their ambivalent articulation of relationships of control, training, care, violence, and love—a protopolitics evident in imaginative play. And, in postnatural media culture, the toy animal has migrated to digital habitats, offering an alternative animal perspective on questions of artificial intelligence. The child's toy and media environment is playfully zoomorphic, populated with artificial animals, from toys and stories to virtual pets and video-game characters, a new simulacral and postnatural trajectory in the descendance of the artificial animal and its playful and play-like behaviors.

The bone marrow of mouse-rat chimeras contains progenitors of multiple pulmonary cell lineages.

Radiation-induced lung injury (RILI) is a common complication of anti-cancer treatments for thoracic and hematologic malignancies. Bone marrow (BM) transplantation restores hematopoietic cell lineages in cancer patients. However, it is ineffective in improving lung repair after RILI due to the paucity of respiratory progenitors in BM transplants. In the present study, we used blastocyst injection to create mouse-rat chimeras, these are artificial animals in which BM is enriched with mouse-derived progenitor cells. FACS-sorted mouse BM cells from mouse-rat chimeras were transplanted into lethally irradiated syngeneic mice, and the contribution of donor cells to the lung tissue was examined using immunostaining and flow cytometry. Donor BM cells provided long-term contributions to all lung-resident hematopoietic cells which includes alveolar macrophages and dendritic cells. Surprisingly, donor BM cells also contributed up to 8% in pulmonary endothelial cells and stromal cells after RILI. To identify respiratory progenitors in donor BM, we performed single-cell RNA sequencing (scRNAseq). Compared to normal mouse BM, increased numbers of hematopoietic progenitors were found in the BM of mouse-rat chimeras. We also identified unique populations of hemangioblast-like progenitor cells expressing Hes1, Dntt and Ebf1, along with mesenchymal stromal cells expressing Cpox, Blvrb and Ermap that were absent or ultra-rare in the normal mouse BM. In summary, by using rats as "bioreactors", we created a unique mouse BM cell transplant that contributes to multiple respiratory cell types after RILI. Interspecies chimeras have promise for future generations of BM transplants enriched in respiratory progenitor cells.

Bugs in the Data: How ImageNet Misrepresents Biodiversity

ImageNet-1k is a dataset often used for benchmarking machine learning (ML) models and evaluating tasks such as image recognition and object detection. Wild animals make up 27% of ImageNet-1k but, unlike classes representing people and objects, these data have not been closely scrutinized. In the current paper, we analyze the 13,450 images from 269 classes that represent wild animals in the ImageNet-1k validation set, with the participation of expert ecologists. We find that many of the classes are ill-defined or overlapping, and that 12% of the images are incorrectly labeled, with some classes having >90% of images incorrect. We also find that both the wildlife-related labels and images included in ImageNet-1k present significant geographical and cultural biases, as well as ambiguities such as artificial animals, multiple species in the same image, or the presence of humans. Our findings highlight serious issues with the extensive use of this dataset for evaluating ML systems, the use of such algorithms in wildlife-related tasks, and more broadly the ways in which ML datasets are commonly created and curated.

Serum-free alginate-C2C12 cells microcapsule as a model of alternative animal protein source.

Due to the climate change crisis, and environmental impacts of the traditional meat sector, the production of artificial animal protein based on in vitro cell culture technology is proposed as an alternative. Furthermore, since traditional animal serum-supplemented cultures pose scientific challenges such as batch variation and contamination risks, artificial animal protein cultures are currently in urgent need of not only serum-free cultures, but also microcarrier culture systems for scalability. However, serum-free microcarrier-based culture system for the differentiation of muscle cells is not available to date. Therefore, we established an edible alginate microcapsules culture system for the differentiation of C2C12 cells in serum-free conditions. Furthermore, metabolites related to central carbon metabolism were profiled based on targeted metabolomics using mass spectrometry. The C2C12 cells cultured in alginate microcapsules displayed high viability throughout 7 days and successfully differentiated within 4 days in serum and serum-free cultures except for AIM-V cultures, which was confirmed by CK activity and MHC immunostaining. Lastly, to the best of our knowledge, this is the first report to compare metabolite profiles between monolayer and alginate microcapsule culture systems. Alginate microcapsule culture showed higher levels of intracellular glycolysis and TCA cycle intermediates, lactate, and the contribution of essential amino acids compared to the monolayer culture. We believe our serum-free alginate microcapsule culture system is adaptable to different species of muscle cells and contributes to future food technology as a proof of concept for the scalability of alternative animal protein source production.

Responses of Land-Use Changes to Drought and its Disparate Impact on Livelihoods of Farmers and Herders in the Agro-Pastoral Ecotone of Northwestern China

The agro-pastoral ecotone is an ecologically fragile region where drought is the main factor influencing land use and livelihoods. In this paper, we took two farmer villages and two herder villages in Ar Horqin Banner, located in the agro-pastoral ecotone of northwestern China, as the research areas, and where we conducted participatory rural appraisal and questionnaire survey to analyze the responses of land-use changes to drought and its disparate impact on the livelihoods of farmers and herders. Results show that: 1) Under drought, farmers tended to abandon rain-fed land, and herders tended to abandon grassland. 2) The livelihood activities of farmers were more stable than those of herders under drought. Farmers abandoned rain-fed farming, and herders just retained cattle rearing. The per capita net income of each farmer in Pingandi and Fenghuangling in the drought year of 2016 was only 9.27% and 12.52% lower than those in 2012, respectively, which was 132.88% and 128.25% lower than those in 2012 of each herder in Wuriduhubu and Haolibao. 3) Diversified livelihoods, especially non-agricultural ones, are the key to ensuring the sustainable livelihoods of farmers and herders. It is an effective way for farmers to encourage more labor force to emigrate to non-agricultural sectors. Regarding herders, it is urgent to develop artificial pastures and animal products processing industry with the support of government.

The third AI summer: AAAI Robert S. Engelmore Memorial Lecture

The third AI summer: AAAI Robert S. Engelmore Memorial Lecture

Thiel embalming in neonates: methodology and benefits in medical training

Current teaching and training methods for surgical techniques in the pediatric population involve artificial models (manikins), animals or adult human cadavers embalmed using various techniques. We found no references in the literature concerning the use of the Thiel method in the pediatric population. The aim of this study, therefore, was to assess the viability of using pediatric human cadavers embalmed through Thiel’s technique and to compare them with standard pediatric manikins. After donation of a 24-week stillborn, the Thiel technique was carried out for fixation following the usual protocol. A video recording with eye-tracking glasses was used to perform an examination, and techniques. The same procedures were conducted on a pediatric manikin. Medical students, medical residents and physicians were asked to respond to questions in an online survey after being shown the video. A total of 92 responses were obtained. The Thiel-embalmed stillborn was assessed as superior to the manikin in all items. Our study confirmed that this technique is feasible even with extremely small donors. The value of this form of preservation for medical training is not widely known though it is receiving increasing interest. Our results show that Thiel fixation in pediatrics is clearly more highly valued than a manikin and offers great potential. This innovative application of the Thiel method in the pediatric population is technically possible. It poses no additional difficulties and is very positively assessed for undergraduate and postgraduate teaching.

Book review – Wildlife Tourism Futures. Encounters with Wild, Captive and Artificial Animals

Book review – Wildlife Tourism Futures. Encounters with Wild, Captive and Artificial Animals

Visual features drive the category-specific impairments on categorization tasks in a patient with object agnosia

Object and scene recognition both require mapping of incoming sensory information to existing conceptual knowledge about the world. A notable finding in brain-damaged patients is that they may show differentially impaired performance for specific categories, such as for “living exemplars”. While numerous patients with category-specific impairments have been reported, the explanations for these deficits remain controversial. In the current study, we investigate the ability of a brain injured patient with a well-established category-specific impairment of semantic memory to perform two categorization experiments: ‘natural’ vs. ‘manmade’ scenes (experiment 1) and objects (experiment 2). Our findings show that the pattern of categorical impairment does not respect the natural versus manmade distinction. This suggests that the impairments may be better explained by differences in visual features, rather than by category membership. Using Deep Convolutional Neural Networks (DCNNs) as ‘artificial animal models’ we further explored this idea. Results indicated that DCNNs with ‘lesions’ in higher order layers showed similar response patterns, with decreased relative performance for manmade scenes (experiment 1) and natural objects (experiment 2), even though they have no semantic category knowledge, apart from a mapping between pictures and labels. Collectively, these results suggest that the direction of category-effects to a large extent depends, at least in MS′ case, on the degree of perceptual differentiation called for, and not semantic knowledge.

What's not in the news headlines or titles of Alzheimer disease articles? #InMice.

There is increasing scrutiny around how science is communicated to the public. For instance, a Twitter account @justsaysinmice (with 70.4K followers in January 2021) was created to call attention to news headlines that omit that mice, not humans, are the ones for whom the study findings apply. This is the case of many headlines reporting on Alzheimer disease (AD) research. AD is characterized by a degeneration of the human brain, loss of cognition, and behavioral changes, for which no treatment is available. Around 200 rodent models have been developed to study AD, even though AD is an exclusively human condition that does not occur naturally in other species and appears impervious to reproduction in artificial animal models, an information not always disclosed. It is not known what prompts writers of news stories to either omit or acknowledge, in the story's headlines, that the study was done in mice and not in humans. Here, we raised the hypothesis that how science is reported by scientists plays a role on the news reporting. To test this hypothesis, we investigated whether an association exists between articles' titles and news' headlines regarding the omission, or not, of mice. To this end, we analyzed a sample of 623 open-access scientific papers indexed in PubMed in 2018 and 2019 that used mice either as models or as the biological source for experimental studies in AD research. We found a significant association (p < 0.01) between articles' titles and news stories' headlines, revealing that when authors omit the species in the paper's title, writers of news stories tend to follow suit. We also found that papers not mentioning mice in their titles are more newsworthy and significantly more tweeted than papers that do. Our study shows that science reporting may affect media reporting and asks for changes in the way we report about findings obtained with animal models used to study human diseases.

Impacts of Cartoon Animals on Human–Alloanimal Relations

ABSTRACT A majority of animated films incorporate alloanimal characters to some extent, either as primary or secondary protagonists. These characters generate interest in the alloanimals they represent, which have been shown to have a variety of effects such as increased visits to zoos, conservation donations, pet buying, and even animal trafficking. This research seeks to establish a general framework for understanding the potential effects these characters can have on human–alloanimal relations. By employing qualitative analyses, we first explore the affective design factors behind these characters. From there, we examine the sign function of these characters using Paul Wells’ schemata of bestial ambivalence and Dagmar Shmauks’ typology of artificial animals. The associated sign-process of these characters and the consideration for alloanimal Umwelten result in what we introduce as the “Expected Animal.” We then go on to explain how “Expected Animals” create imagined human–alloanimal relations which can impact real-world interactions between humans and other animals. This research offers insights into understanding the ecosemiotic impacts of cartoon animals, which can be used by animation studios to better mediate positive human–animal interactions with the release of their films.

Pouring petrol on the flames: Using oncolytic virotherapies to enhance tumour immunogenicity.

Oncolytic viruses possess the ability to infect, replicate and lyse malignantly transformed tumour cells. This oncolytic activity amplifies the therapeutic advantage and induces a form of immunogenic cell death, characterized by increased CD8 + T‐cell infiltration into the tumour microenvironment. This important feature of oncolytic viruses can result in the warming up of immunologically ‘cold’ tumour types, presenting the enticing possibility that oncolytic virus treatment combined with immunotherapies may enhance efficacy. In this review, we assess some of the most promising candidates that might be used for oncolytic virotherapy: immunotherapy combinations. We assess their potential as separate agents or as agents combined into a single therapy, where the immunotherapy is encoded within the genome of the oncolytic virus. The development of such advanced agents will require increasingly sophisticated model systems for their preclinical assessment and evaluation. In vivo rodent model systems are fraught with limitations in this regard. Oncolytic viruses replicate selectively within human cells and therefore require human xenografts in immune‐deficient mice for their evaluation. However, the use of immune‐deficient rodent models hinders the ability to study immune responses against any immunomodulatory transgenes engineered within the viral genome and expressed within the tumour microenvironment. There has therefore been a shift towards the use of more sophisticated ex vivo patient‐derived model systems based on organoids and explant co‐cultures with immune cells, which may be more predictive of efficacy than contrived and artificial animal models. We review the best of those model systems here.

Investigation of the dyeing properties of wool fabrics with Alkanna tinctoria root extract

In this work, dyeing properties of wool samples with Alkanna tinctorum root extract was investigated. For this purpose, two different dyestuff extracts, red and blue are gained from its roots with acetone and ethanol solvents, as well. Wool fabrics (194 pieces) dyed with AIK(SO4)·12H2O, FeSO4·7H2O, CuSO4·5H2O, AgNO3 and CoCI2·6H2O mordants at pH 2, 4, 6 and 8, respectively. Unmordanting, pre-mordanting, meta-mordanting, and post-mordanting methods were used for the dyeing experiments. In addition, wool fabrics were pretreated with artificial animal urine system (AAUS) including NH3 (3%, v/v), CaC2O4 (3%, m/v) and urea (3%, m/v) before dyeing processes in order to see the improvement in the fastness properties of the dyed samples with meta-mordanting method. The color codes were determined with Pantone Color Guide, and also washing-, rubbing fastness levels were evaluated using gray scale. According to the evaluations, it is observed that AAUS mordant system helps to improve the light fastness values in general. Good results were obtained both for the dyeings with red and blue components, as well. Optimum dyeing parameters were also evaluated for wool fabric in terms of usage in textile industry.

The Tiger Penis Project

Many cultures have their own systems of alternative medicine, the effectiveness of which cannot always be proven according to contemporary scientific analysis; the use of the tiger penis to increase virility in traditional Chinese medicine (TCM) is one such practice. While TCM may offer benefits beyond those available through mainstream western medicines, the huge demand for wild animals in TCM poses a threat to endangered species. Can a new interpretation of TCM resolve the conflict between health, culture, and environmental conservation? The Tiger Penis Project proposes the use of emerging biotechnologies to create artificial animal parts for Chinese medical applications both to prevent the further destruction of animals and traditional cultures and to provide more possibilities for the coexistence of human society and the natural environment.

Study of a new nano-hydroxyapatite/basic fibroblast growth factor composite promoting periodontal tissue regeneration

Tissue engineering technology provides a new method for periodontal regeneration. Finding or preparing a suitable scaffold is the key to periodontal tissue engineering. Here, we use nano-hydroxyapatite-modified collagen biomimetic material (nHAC) as the packaging material, and carry fibroblast growth factor (bFGF) for the regeneration and repair of periodontal tissue. Due to its low cytotoxicity and high biocompatibility, nHAC shows unique advantages in the construction of periodontal tissue scaffolds. The nHAC periodontal tissue scaffold material has a dense and porous three-dimensional network structure, has a high loading rate of bFGF, and can firmly lock human periodontal ligament cells (HPDLCs), which is easy for cell growth and attachment. In vivo experiments have shown that, in artificial animal periodontal tissue models, the nHAC-loaded bFGF periodontal scaffold covered by Geistlich Bio-Gide (GBG) membrane is better than the simple GBG membrane and the blank group, the nHAC/bFGF-GBG composite membrane It is beneficial to promote the growth of new alveolar bone and cement formation, and realize the regeneration of periodontal tissue.

Short- and long-term effects of rearing dairy calves with contact to their mother on their reactions towards humans.

In this Research Communication we address the hypotheses that reduced contact with humans during the first week of life would impair the relationship of dairy calves reared in dam-calf-contact systems to humans in comparison with artificially reared animals, but that this difference would vanish over time. Artificially reared calves (Artificial) that had been separated from their mother within 12 h after birth were bottle-fed with colostrum for 5 d and thereafter sucked milk from an automatic milk feeder. Animals reared with dam-calf contact (Dam-contact) were kept in the calving pen with their dam for 5 d, and then had permanent access to the cow barn and thus to their dam. Calves were weaned at an age of 12 weeks and kept in young stock groups mixed of both treatments until integration into the cow herd. We tested the animals' relationship with humans by assessing the animals' responses towards an unfamiliar person in an avoidance distance (AD) test in the home environment at 4 weeks of age, at 15 months and at 33 months. In calves, we additionally measured AD in a novel arena after a stationary person test. Artificial animals had lower AD, i.e. showed lower level of fear, than Dam-contact calves. However, the AD in Dam-contact calves decreased with increasing number of days they experienced assistance for suckling. Further, there was no significant difference in later ages. In conclusion, gentle human contact in combination with feeding during the first 5 d of life improved calves' relationship to humans leading to differences between the two treatments as well as within the Dam-contact calves. Potential effects under different conditions regarding quantity and quality of human-animal interactions need further research.

A three-dimensional intestinal tissue model reveals factors and small regulatory RNAs important for colonization with Campylobacter jejuni.

The Gram-negative Epsilonproteobacterium Campylobacter jejuni is currently the most prevalent bacterial foodborne pathogen. Like for many other human pathogens, infection studies with C. jejuni mainly employ artificial animal or cell culture models that can be limited in their ability to reflect the in-vivo environment within the human host. Here, we report the development and application of a human three-dimensional (3D) infection model based on tissue engineering to study host-pathogen interactions. Our intestinal 3D tissue model is built on a decellularized extracellular matrix scaffold, which is reseeded with human Caco-2 cells. Dynamic culture conditions enable the formation of a polarized mucosal epithelial barrier reminiscent of the 3D microarchitecture of the human small intestine. Infection with C. jejuni demonstrates that the 3D tissue model can reveal isolate-dependent colonization and barrier disruption phenotypes accompanied by perturbed localization of cell-cell junctions. Pathogenesis-related phenotypes of C. jejuni mutant strains in the 3D model deviated from those obtained with 2D-monolayers, but recapitulated phenotypes previously observed in animal models. Moreover, we demonstrate the involvement of a small regulatory RNA pair, CJnc180/190, during infections and observe different phenotypes of CJnc180/190 mutant strains in 2D vs. 3D infection models. Hereby, the CJnc190 sRNA exerts its pathogenic influence, at least in part, via repression of PtmG, which is involved in flagellin modification. Our results suggest that the Caco-2 cell-based 3D tissue model is a valuable and biologically relevant tool between in-vitro and in-vivo infection models to study virulence of C. jejuni and other gastrointestinal pathogens.

Combining Evolution and Learning in Computational Ecosystems

Abstract Although animals such as spiders, fish, and birds have very different anatomies, the basic mechanisms that govern their perception, decision-making, learning, reproduction, and death have striking similarities. These mechanisms have apparently allowed the development of general intelligence in nature. This led us to the idea of approaching artificial general intelligence (AGI) by constructing a generic artificial animal (animat) with a configurable body and fixed mechanisms of perception, decision-making, learning, reproduction, and death. One instance of this generic animat could be an artificial spider, another an artificial fish, and a third an artificial bird. The goal of all decision-making in this model is to maintain homeostasis. Thus actions are selected that might promote survival and reproduction to varying degrees. All decision-making is based on knowledge that is stored in network structures. Each animat has two such network structures: a genotype and a phenotype. The genotype models the initial nervous system that is encoded in the genome (“the brain at birth”), while the phenotype represents the nervous system in its present form (“the brain at present”). Initially the phenotype and the genotype coincide, but then the phenotype keeps developing as a result of learning, while the genotype essentially remains unchanged. The model is extended to ecosystems populated by animats that develop continuously according to fixed mechanisms for sexual or asexual reproduction, and death. Several examples of simple ecosystems are given. We show that our generic animat model possesses general intelligence in a primitive form. In fact, it can learn simple forms of locomotion, navigation, foraging, language, and arithmetic.

The Research Advance of Traditional Meat Substitutes-Artificial Meat

Artificial meat is mainly divided into two types. One is artificial animal meat synthesized by animal stem cells,and the other is plant meat synthesized by plant protein and other botanical ingredients. From an energy perspective,artificial meat can reduce the energy consumption of raising livestock. From the perspective of greenhouse effect,artificial meat can reduce greenhouse gas emissions and reduce global warming stress. From a nutritional perspective,artificial animal meat can provide amino acids,fats,and vitamins similar to traditional slaughter meat. Plant-based meat is mainly composed of plant protein,which can also provide proteins to humans. Therefore,artificial meat as a substitute for traditional meat can not only provide the nutrients,but also bring sustainable benefits to the environment and energy. However,artificial meat still has many problems in technology,ethics and safety. This paper introduces two main methods of artificial meat production,reviewed people’s current views and attitudes on artificial meat,and finally summarizes the challenges that artificial meat may encounter in the future market. It is expected that this review can provide reference for the development of China’s artificial meat industry.

Perception of artificial conspecifics by bearded dragons (Pogona vitticeps).

Artificial animals are increasingly used as conspecific stimuli in animal behavior research. However, researchers often have an incomplete understanding of how the species under study perceives conspecifics, and, hence, which features are needed for a stimulus to be perceived appropriately. To investigate the features that bearded dragons (Pogona vitticeps) attend to, we measured their lateralized eye use when assessing a successive range of stimuli. These ranged through several stages of realism in artificial conspecifics, to see how features such as color, the presence of eyes, body shape and motion influence behavior. We found differences in lateralized eye use depending on the sex of the observing bearded dragon and the artificial conspecific, as well as the artificial conspecific's behavior. Therefore, this approach can inform the design of robotic animals that elicit biologically-meaningful responses in live animals.