Study of a new nano-hydroxyapatite/basic fibroblast growth factor composite promoting periodontal tissue regeneration

Abstract

Tissue engineering technology provides a new method for periodontal regeneration. Finding or preparing a suitable scaffold is the key to periodontal tissue engineering. Here, we use nano-hydroxyapatite-modified collagen biomimetic material (nHAC) as the packaging material, and carry fibroblast growth factor (bFGF) for the regeneration and repair of periodontal tissue. Due to its low cytotoxicity and high biocompatibility, nHAC shows unique advantages in the construction of periodontal tissue scaffolds. The nHAC periodontal tissue scaffold material has a dense and porous three-dimensional network structure, has a high loading rate of bFGF, and can firmly lock human periodontal ligament cells (HPDLCs), which is easy for cell growth and attachment. In vivo experiments have shown that, in artificial animal periodontal tissue models, the nHAC-loaded bFGF periodontal scaffold covered by Geistlich Bio-Gide (GBG) membrane is better than the simple GBG membrane and the blank group, the nHAC/bFGF-GBG composite membrane It is beneficial to promote the growth of new alveolar bone and cement formation, and realize the regeneration of periodontal tissue.