Chao Lake is a Geoheritage site on the active Tan-Lu Fault between the Yangtze craton, the North China craton, and the Dabie orogenic belt in the southeast. This segment of the fault is not well constrained at depth partly due to the overprinting of the fault zone by intrusive materials and its relatively low seismic activity and sparse seismic station coverage. This study took advantage of a dense seismic array deployed around Chao Lake to delineate the P-wave velocity variations in the crust and uppermost mantle using teleseismic earthquake arrival time tomography. The station-pair double-difference with waveform cross-correlation technique was employed. We used a multiscale resolution 3-D initial model derived from the combination of high-resolution 3-D vS models within the region of interest to account for the lateral heterogeneity in the upper crust. The results revealed that the velocity of the upper crust is segmented with structures trending in the direction of the strike of the fault. Sedimentary basins are delineated on both sides of the fault with slow velocities, while the fault zone is characterized by high velocity in the crust and uppermost mantle. The high-velocity structure in the fault zone shows characteristics of magma intrusion that may be connected to the Mesozoic magmatism in and around the Middle and Lower Yangtze River Metallogenic Belt (MLYMB), implying that the Tan-Lu fault might have formed a channel for magma intrusion. Magmatic material in Chao Lake is likely connected to the partial melting, assimilation, storage, and homogenization of the uppermost mantle and the lower crustal rocks. The intrusions, however, seem to have suffered severe regional extension along the Tan-Lu fault driven by the eastward Paleo-Pacific plate subduction, thereby losing its deep trail due to extensional erosion.