Areca Nut Extract Research Articles

Stimulation of MMP-9 of oral epithelial cells by areca nut extract is related to TGF-β/Smad2-dependent and -independent pathways and prevented by betel leaf extract, hydroxychavicol and melatonin.

Background: There are 200-600 million betel quid (BQ) chewers in the world. BQ increases oral cancer risk. Matrix metalloproteinase-9 (MMP-9) is responsible for matrix degradation, cancer invasion and metastasis. Whether areca nut extract (ANE), a BQ component, stimulates MMP-9 secretion, and the related signaling pathways awaits investigation.Results: ANE (but not arecoline) stimulated MMP-9 production of gingival keratinocytes and SAS cancer epithelial cells. ANE stimulated TGF-β1, p-Smad2, and p-TAK1 protein expression. ANE-induced MMP-9 production/expression in SAS cells can be attenuated by SB431542 (ALK5/Smad2 inhibitor), 5Z-7-Oxozeaenol (TAK1 inhibitor), catalase, PD153035 (EGFR tyrosine kinase inhibitor), AG490 (JAK inhibitor), U0126 (MEK/ERK inhibitor), LY294002 (PI3K/Akt inhibitor), betel leaf (PBL) extract, and hydroxychavicol (HC, a PBL component), and melatonin, but not by aspirin.Conclusions: AN components contribute to oral carcinogenesis by stimulating MMP-9 secretion, thus enhancing tumor invasion/metastasis. These events are related to reactive oxygen species, TGF-β1, Smad2-dependent and –independent signaling, but not COX. These signaling molecules can be biomarkers of BQ carcinogenesis. PBL, HC and melatonin and other targeting therapy can be used for oral cancer treatment.Methods: ANE-induced MMP-9 expression/secretion of oral epithelial cells and related TGF-β1, Smad-dependent and –independent signaling were studied by MTT assay, RT-PCR, western blotting, immunofluorescent staining, and ELISA.

Treatment with the anti-diabetic drug metformin ameliorates betel-nut induced carcinogenesis in a murine model.

Metformin, a widely used anti-diabetic drug has gained enormous attention as an anticancer agent. This study seeks to investigate the efficacy of metformin in ameliorating aqueous extract of betel-nut (AEBN) and arecoline induced carcinogenesis in a murine model. Swiss albino mice were exposed to AEBN (2 mg ml-1) and arecoline (10 μg ml-1) in drinking water for 16 weeks followed by co-administration of metformin (75 mg kg-1 or 150 mg kg-1) for 4 or 8 weeks. Histological changes and oxidative stress were assessed by haematoxylin and eosin staining, TBARS assay and protein carbonylation assay respectively. Lipid profile was determined using an automated analyzer. Expression of total and phosphorylated AMPK, ACC and p53 were determined by immunoblotting. AEBN and arecoline induced dyslipidemia by downregulating AMPK (Thr-172) and activating ACC (Ser-79); they also downregulated tumor suppressor p53 (Ser-15). Metformin treatment induced AMPK-dependent alleviation of dyslipidemia in a dose and time dependent manner, upregulated p53 (Ser-15), restored tissue architecture and reduced oxidative stress in tissues of AEBN and arecoline treated mice. This study establishes that betel nut induces dyslipidemia through its alkaloid, arecoline by inhibition of AMPK (Thr-172) and activation of ACC (Ser-79) and highlights the therapeutic potential of metformin for treatment of betel-nut induced carcinogenesis, indicating the repurposing of the old drug in a new avenue.

Aktivitas Antimikroba Ekstrak Etanol Biji Pinang Muda (Areca catechu)

Thrush is an oral disease caused by bacterial and fungal infections. Treatment for this disease can be done by administering drugs that contain antimicrobial compounds. This study aims to test the ethanol extract of young areca nut against bacteria and fungi that cause Oral thrush. Young betel nut extract is obtained through maceration process using 96% ethanol. Then the activity of the Streptococcus sanguis bacteria and Candida albican fungus was tested. This test used the well method which was carried out on 5 variations of the concentration of young areca nut extract (2.5%; 5%; 7.5%; 10% and 15%) with one negative control (Na CMC). The results of the antimicrobial activity of young areca nut extract showed that young areca nut extract can inhibit Streptococcus sanguis bacteria with inhibition value are (9,961; 11,319; 13,390; 14,184; 14,704 mm) and Candida albican fungus are (7,482; 10,923; 14,695; 15,742; 17,325 mm) on 5 variations of the concentration.

The use of natural Areca catechu dyes for silk and nylon and its halochromic effect

In this study, we have investigated the use of betel nut (Areca catechu) extract solution as a natural dye for the use of dyeing for silk and nylon. Chemical structure of betel nut extracted solution was confirmed by using FTIR and 1H NMR spectroscopy and thermal properties also measured for determining the proper dyeing temperature. In order to optimise the colouration properties, variable dyeing parameters (temp, pH and mordant type) was studied. The halochromic effect of synthesised natural dye was analysed by using UV–Vis spectroscopy. Additionally dye build-up, colour fastness properties of both fabrics were compared.

Color Masking of Betel Nut Extract Using Encapsulation Technology for Nutricosmeceutical Applications

In this work, encapsulation technology—nanoemulsions (NEs) and nanostructured lipid carriers (NLCs)—was applied for color masking of betel nut extract for oral and skin applications. Types and extents of surfactant and lipid as well as homogenization process showed essential roles on physical properties of the encapsulated betel nut extract. Comparing to solution of betel nut extract in methanol, the degree of lightness of the optimized NE and NLC formulations improved more than 44 time compared to unencapsulated formulation. The optimized NE and NLC were most stable at 25 °C. Extreme storage conditions of 4 and 45 °C resulted in phase separation and color change, respectively. However, total phenolic and antioxidant activity were found stable during the stability test in all storage conditions. This work has shown a great potential use of nanoencapsulation technology in color masking plus maintain activities of betel nut extract for food as well as cosmetic application.

ISOLASI DAN UJI AKTIVITAS DAYA HAMBAT EKSTRAK ETANOL BIJI PINANG (Areca catechu L.) TERHADAP BAKTERI PADA LIDAH

Betel nut (Areca catechu L.) is a natural material that has antibacterial activity in treating diseased on teeth, halitosis and can inhibit of dental caries. The objective of this research is type of bacteria based on morphology, the results of bacterial identification tests on the tongue and to determine the effect of the inhibitory power of betel nut ethanol extract (Areca catechu L.) to bacteria of the tongue. Antibacterial activity testing using disc diffusion method with various concentrations of 10%, 20% and 30%. Secondary metabolite contents are alkaloid, terpenoid and flavonoid.The results obtained from the identification test based on the color of bacteria Branhamella catarrhalis, Staphylococcus epidermidis and Straphylococcus aureus. Ethanol extract of areca nut (Areca catechu L.) is better to inhibiting the growth of Staphylococcus epidermidis and Staphylococcus aureus compared to Branhamella catarrhalis. Based on Two Way Anova statistical data on inhibitory zone diameters, there were differences between the concentration groups of 10%, 20% and 30% and there were significant differences in the inhibitory activity of Branhamella catarrhalis bacteria compared to Staphylococcus epidermidis and Straphylococcus aureus bacteria but not significantly different between Staphylococcus epidermidis and Straphylococcus aureus bacteria

Biosynthesis of copper nanoparticles from areca nut extract and its antibacterial and antioxidant properties

The phytochemicals present in different maturity stages of areca nut (Areca catechu) were evaluated. Phytochemical tests revealed the presence of carbohydrates, alkaloids, flavonoids, tannins and phenolic compounds. Copper nanoparticles were biosynthesized from the matured areca nut extract and characterization of the synthesized nanoparticles was carried out by analyzing the surface plasmon resonance, particle size and zeta potential. Field emission scanning electron microscopy and Energy dispersive X-ray analysis (EDAX) of nanoparticles synthesized from only stage 5 areca nut extract were used because this stage produced the highest antibacterial activity. The antibacterial activity of nanoparticles and the extract was tested against Gram-positive and Gram-negative bacteria; the plain extract had a zone of inhibition of 10–13 mm, whereas biosynthesized copper nanoparticles had a zone of inhibition of 23–25 mm. The nanoparticles also had enhanced antioxidant activity compared to plain areca nut extract and thus, the extract has potential for use as an antioxidant agent in pharmaceuticals and cosmetics. It can be concluded from this study that by using a nanotechnological approach, phytochemicals found in areca nut could be put to good use to enhance their antibacterial and antioxidant activity.

Betel nut (Areca catechu L.) extract toxicity on the kidney and liver of white rats (Rattus norvegicus)

Background: Medication using herbal medicine is one of the alternatives chosen to reduce the adverse effects caused by synthetic drugs. Betel nut (Areca catechu L) usually used as an alternative drug such as anthelmintic and others.Aim: The study aims to determine the chronic toxicity effect of betel nut.Method: Five hundred grams of simplicial powder was macerated using 95% ethanol in a container, then sealed and let them for three days. They were protected from light while repeatedly stirred. Rat was given Betel nut extract for three weeks. The histopathological examination was observed under the microscope.Result: The mean GOT serum, GPT serum, BUN, and creatinine serum levels between control (no extract given) and each dose, i.e., 0.2 ccs, 0.4 ccs, and 0.8 ccs, showed insignificant results (p > 0.05). According to the histopathological observation, there was no difference between control and treatment.Conclusion: Various orally doses of betel nut extract did not cause toxicity in white rats (rattus novergicus).Â

Copper containing fungicide – the actual culprit in the pathogenesis of OSMF?

Introduction: To estimate possible role of copper in etiology of oral sub mucus fibrosis. Aims and Objectives: To analyse and compare metal constituents of arecanuts sprayed with copper containing fungicide and that not sprayed with fungicide. To evaluate the effects of arecanuts sprayed with copper containing fungicide on the buccal mucosa of rats. Materials and Methods: Aquous solutions of arecanuts sprayed and not sprayed with fungicide, were prepared and sent to NITK for metal analyses. Buccal mucosa of 6 adult Wistar albino rats, were treated twice daily, for 6 days a week, with topical application of aqueous areca nut extracts, for one year. A control group (n = 6) was treated with distilled water. Biopsy was conducted at the end of six, nine and twelve months. The influence of areca nut on the oral epithelium and connective tissue of the rats were recorded semi quantitatively by light microscopy. Results: Metal analysis showed that the difference of copper content of arecanut treated with fungicide when compared to that of those not treated with fungicide was very meagre. The histopathological report of the tissue treated with arecanut solution showed development of fibrosis even at the 6th month. Though not uniformly progressive, fibrosis was noticed in all the tissues at 6, 9 and 12 months. Conclusion: The copper content of arecanut treated with fungicide and that not treated with fungicide was not very significant. The arecanut treated with copper containing fungicide can induce OSMF like changes in rat mucosa. Keywords: Arecanut, Copper containing fungicide, OSMF, Rat mucosa.

Areca nut extract demonstrated apoptosis-inducing mechanismby increased caspase-3 activities on oral squamous cell carcinoma.

Background: Oral squamous cell carcinoma is a neoplasm of keratinocyte cells of oral mucosa epithelium that can potentially spread through lymphatic tissue or blood vessel. Although areca nut is one of the plants with a risk of inducing that cancer, areca nut is believed to have high antioxidant properties. Due to the current interest in the apoptosis effects from areca nut for oral cancer treatment, we investigated its ability to induce apoptosis and caspase-3 activity in oral cancer cell lines: HSC-2 and HSC-3. Methods: We examined the effect of areca nut on apoptosis and caspase-3 activity in HSC-2 and HSC-3 cells. Flow cytometry was conducted for the quantification of the cells that were apoptotic and expressing the caspase-3 enzyme for 24 and 48 hours. Results: Areca nut induced a significant increase (p<0.01) in late apoptosis of HSC-2 cells and mostly occurred over 48 hours. The study also found that in HSC-3, there weresignificant increases (p<0.01) the percentage of cells in early apoptosis after 24 hours and late apoptosis at 48 hours. Caspase-3 activity increased after 24 and 48 hours of areca nut exposure in both cells. Conclusions: The study showed that areca nut could be considered as a potential anticancer agent through its capability in inducing a caspase-dependent apoptosis.

ARECA NUT EXTRACT ENHANCED M2-LIKE MACROPHAGE POLARIZATION AND fiBROBLAST ACTIVATION

Objectives Areca nut chewing habit is popular in Taiwan and is closely related to oral squamous cell carcinoma (OSCC). Both activated fibroblasts expressing alpha-smooth muscle actin (α-SMA) and tumor-associated macrophages showing M2 polarization in stroma are supposed to be crucial in tumor progression. The purpose of the study was to examine the profile of stromal fibroblasts and macrophages in areca-associated oral cancer tissues, thein viro effects of areca nut extract (ANE) and two common oral insults, nicotine (NT) and lipopolysaccharides (LPS), on primary oral fibroblasts and human macrophages were also investigated. The study was approved by institutional Review Board of Taipei Veterans General Hospital, Taipei, Taiwan. Oral tissues were obtained with informed consent from patients undergoing routine surgical treatment. Findings Tissue sections showed that compared to the tumor-adjacent normal tissues (ANT), OSCC revealed a higher expression of pan-macrophage marker CD68, M2 markers CD163 and arginase-1 and activated fibroblast marker α-SMA, but not M1 marker CD86. In in vitro cell experiments, all of ANE, NT and/or LPS treatments could increase α-SMA expression and collagen production by oral fibroblasts. But only ANE treatment group, not NT or LPS group, enhanced the expression of M2 marker arginase-1 by macrophages. Furthermore, conditioned media acquired from macrophages (CM-Mac) of ANE treatment group increased the collagen production and IL-6 secretion by fibroblasts. CM-Mac of LPS treatment group also increased IL-6 secretion. Taken together, fibroblasts could be activated by ANE, NT and LPS, but only ANE could enhance macrophage M2-like polarization which in turn further increased fibroblast protein production. Conclusions Areca nut might compromise oral health by the setup of tumor-promoting microenvironment with local immune dysregulation via the enrichment of activated fibroblasts and M2-like macrophages.

The Difference Between Gargling Using Betel Nut Seed (Areca Catechu) Extract and Chlorhexidine 0.2% Solution in Chronic Gingivitis Healing

The use of mouthwash is an effective way to help cure gingivitis. Mouthwash that is often used is mouthwash containing antiseptic. Recently, there has been increasing use of traditional medicines that are considered safer such as areca nuts compared to chemically synthesized agents. The aim of this study was to calculate the difference in the healing of chronic gingivitis due to calculus between gargling using areca nut (Areca catechu) extract and using Chlorhexidine 0.2% solution. This was an experimental test with pre and post-test control group design and involved two treatment groups. A total of 30 subjects with grade 2 gingivitis were being admitted. The first group was given mouthwash with areca nut (Areca catechu) extract and another group with Chlorhexidine 0.2% twice daily for three days. The mean gingival index in the group which gargled using betel nut seed (Areca catechu) extract on the first day was 1,93±0,704 followed by 0,40±0,507 on the second day, and 0,00±0,00 on the third day, on the other hand the mean gingival index in the group which gargled using Chlorhexidine 0.2% solution was 1,93±0,704 on the first day, 0,40±0,507 on the second day, and 0,00±0,00 on the third day. The mean gingival index decreased significantly during observation period in both treatment groups but when being compared to each other there was no significant difference in the healing time of chronic gingivitis in group which gargled using betel nut seed extract (Areca catechu) and Chlorhexidine 0.2% solution.

Cytotoxic Potentials of Betel Quid and its Components

In this research, it aims to determine the potential cytotoxic effects of the betel quid, areca nut, betel leaf and slaked lime extracts using Allium cepaassay through evaluating the root length, chromosome aberration and mitotic index. A series of Allium cepabulbs were exposed to varying concentrations together with a known mutagen, colchicine. The treatment groups consist of: 100% and 50% of the betel quid and its components. After 48 hours of exposure, the root tips from the different treatments were processed for cytological studies by aceto-orcein squash technique. The cytotoxic effects of betel quid and its components observed in Alliumcepa root tips showed growth retardation in terms of root length in high concentration. There was a decrease in mitotic index percentage with increasing concentrations. The betel quid and components-induced chromosome aberrations were statistically significant (p&lt;0.05). The result from the cytotoxicity study data revealed that the extracts of the betel quid and its components inhibited the growth of the Allium cepa roots and has potent cytotoxic effects.

APLIKASI PERLAKUAN PENDAHULUAN PULSED ELECTRIC FIELD (PEF) PADA EKSTRAKSI TANIN BIJI PINANG (Areca catechu) (KAJIAN FREKUENSI DAN WAKTU PEF)

Areca catechu contains tannins compounds acting as antioxidants. Extraction process is required to obtain these compounds from areca nut. Pretreatment before extraction using Pulsed Electric Field (PEF) has shown improvement of yield as well as the quality of antioxidant extracts compared with control. The experimental design of this research was Factorial Random Design with two factors. The first factor was frequency of PEF with three levels of 1, 1.5, and 2 kHz and the second factor was PEF application time with three levels of 20, 25, and 30 seconds. The extract of areca seed from PEF extraction with combination of frequency and time of PEF using magnetic field 450 v/cm did not provide significanty different yield and quality. The selection of best treatment was based on the economic aspects and analysis of variance, since there was no significant difference between each parameter and treatment. The best frequency and duration of PEF on yield and antioxidant quality of areca nut extract were 1 kHz and 20 second with a yield of 5.6%; moisture content of 7.78%; IC50 value of 277,56 ppm; and tannins content of 442.36 mg GAE/g. FTIR spectrophotometric analysis results showed that the resulting absorption has a functional group that supports the presence of tannins.Keywords: fresh areca nut, electroporation, electrical pulse

Areca nut extract demonstrated apoptosis-inducing mechanism by increased caspase-3 activities on oral squamous cell carcinoma

Background: Oral squamous cell carcinoma is a neoplasm of keratinocyte cells of oral mucosa epithelium that can potentially spread through lymphatic tissue or blood vessel. Although areca nut is one of the plants with a risk of inducing that cancer, areca nut is believed to have high antioxidant properties. Due to the current interest in the apoptosis effects from areca nut for oral cancer treatment, we investigated its ability to induce apoptosis and caspase-3 activity in oral cancer cell lines: HSC-2 and HSC-3. Methods: We examined the effect of areca nut on apoptosis and caspase-3 activity in HSC-2 and HSC-3 cells. Flow cytometry was conducted for the quantification of the cells that were apoptotic and expressing the caspase-3 enzyme for 24 and 48 hours. Results: Areca nut induced a significant increase (p&lt;0.01) in late apoptosis of HSC-2 cells and mostly occurred over 48 hours. The study also found that in HSC-3, there were significant increases (p&lt;0.01) the percentage of cells in early apoptosis after 24 hours and late apoptosis at 48 hours. Caspase-3 activity increased after 24 and 48 hours of areca nut exposure in both cells. Conclusions: The study showed that areca nut could be considered as a potential anticancer agent through its capability in inducing a caspase-dependent apoptosis.

Stress activated p38 MAPK regulates cell cycle via AP-1 factors in areca extract exposed human lung epithelial cells.

Areca nut chewing habits are associated with several oral manifestations like leukoplakia, submucous fibrosis and oral squamous cell carcinoma. Although numerous evidence on areca toxicity is known but the mechanistic pathway of disease causation is to be studied. Aqueous areca nut extract treated A549 cells showed reduced cell viability by 48h with IC50 value of 0.50%. The toxic nature of areca nut induced the production of reactive oxygen species with decreased anti-oxidant glutathione S transferase levels lead to altered redox homeostasis. PCR studies showed decreased mRNA levels of Jun and Fos AP-1 subunits on extract treatment by 48h. The protein levels of PCNA, CDK4, RB, p53, c-Jun and c-Fos were found to be downregulated with upregulated CDK inhibitor p21 on extract treatment as compared to control. Results of FACS analysis further confirm G1/S phase cell cycle arrest on areca nut extract exposure. The regulation of downstream AP-1 subunits by MAPKs was studied by using specific inhibitors of ERK, JNK and p38 along with areca nut extract. Results showed the redox activation of MAP kinases down regulated the mRNA levels of AP-1 subunits in aqueous areca nut extract treated cells. Hence the present study aids in elucidating the role of MAP kinases in regulating the AP-1 subunits and their implications on target genes that are involved regulation of various cellular processes. Further, it would help in understanding the mechanistic aspects of the diseased state which may facilitate in designing of new therapeutic modalities that could help in cancer management.

Anthelmintic effect of betel nut (Areca catechu) and neem (Azadirachta indica) extract against liver fluke (Fasciola spp.).

Objective:This study aimed to measure the anthelmintic effects of betel nut (Areca catechu) and neem (Azadirachta indica) leaf extracts against Fasciola spp. in vitro in comparison with the commercial dewormer, Albendazole, and the negative control, nutrient broth. The study determined the extract concentration that produced the highest efficacy based on the average recorded mean motility time, gross, and microscopic changes of the flukes treated with different concentrations of plant extracts.Material and Methods:The study consisted of eight treatments. Every treatment consisted of 10%, 20%, and 40% concentrations of both betel nut extract (BNE) and neem leaf extracts, positive control treatment (Albendazole-treated) and negative control treatment (25 ml nutrient broth). The motility of the flukes on all treatments was based on the established motility criteria scoring. The flukes subjected to all treatments were processed for histopathological analysis.Results:The result of the study revealed that after exposure of Fasciola spp. under 10%, 20%, and 40% extract concentrations, betel nut showed higher efficacy having the recorded mean motility time of 0.22, 0.07 min, and no movement upon contact, respectively, than Albendazole which produced mean motility time of 0.38 min. Nevertheless, the flukes treated with 10%, 20%, and 40% neem leaf extracts obtained the average mean motility time of 220, 151, and 98 min, respectively.Conclusions:The results gathered showed that 40% BNE concentration showed the highest efficacy based on the recorded mean motility time. All treatments of betel nut extract evidently showed marked changes in the gross and microscopic morphology of the flukes. However, the neem extract was ineffective in all concentrations although changes were observed microscopically. Furthermore, the nutrient broth was proven to be effective as a culture medium since the flukes remained active until 8 h of exposure.

The Effectiveness of Teak Wood-Sawdust Liquid Smoke and Areca-Nut Extract as a Pesticide on Pomacea canaliculata

The present study was aimed to evaluate the effectiveness of Liquidsmoke (LS), Areca-nut extract (AE) and their combination on restriction of golden apple snail (Pomaceacanaliculata) activity, which is known as a major pest in Indonesian rice farms. The experiment conducted under laboratory conditions and each experimental unit consisted of aplastic jar 13 cm in diameter and 15 cm high, which was coverd with gauze which kept 20 snails and fed 2 g/day of the fresh taro leaves, respectively. The treatments were arranged in a completely randomized design with three replications. The results showed that the treatment using LSconcentration of 15% showed moluscisidal action. LS treatment at concentrations of 5 and 10%; Areca-nut extract (AE) treatment at concentrations of 5,10 and 15%; and a mixture of LS and AE at a concentration of 5.10, and 15% proved not to have a significant effect.

Extraction, Characterization and Identification of Major Chemical Components of Areca Nut Extract at its Different Stages

The medicinal properties shown by different plants are due to phytochemicals present in the plant. These phytochemicals are the most vital source for the treatment of various diseases. Different phytochemicals have an extensive range of activities, which help to enhance the immune system and give resistance to the body to protect against attack of harmful pathogens. It is well accepted fact that even areca plant seed is also a good source of phytochemicals and hence planned to examine the phytochemicals present in its different stages, that is, tender areca nut (TACN), mature areca nut (MACN) and dry areca nut (DACN). All the three stages of areca nut extract were examined for tannin, phenols, flavonoids, alcohols, acids, amines and nitro groups. They showed positive results for all the tests. Trace elements such as Cu, Fe, Zn, Cr, Ni, Pb are present in small amount when compared to Na and K, and are identified by Atomic Adsorption Spectroscopy. FTIR analysis revealed the presence of functional groups such as –OH. –NH, -CH, >C=O, >C=C C-O-C and –NO groups in the areca nut extract. Extracts were investigated through GC-MS for identification of the chemical composition of extract, on comparison with results obtained from FTIR, and molecular mass nine, fourteen and five compounds were identified in TACN, MACN and DACN extracts, respectively.

FORMULASI DAN UJI EFEKTIVITAS ANTIBAKTERI EDIBLE FILM EKSTRAK BIJI PINANG (Areca catechu Linn)

ABSTRACTThe tannins contained in areca nut extract can inhibit the growth of Streptococcus mutans bacteria. To facilitate the use of betel seed extract as an antibacterial, it was formulated into an edible film with a concentration of 2.5%, 3% and 3.5%. Making edible films using solvent casting method and testing antibacterial activity using blood agar diffusion method. The edible film of areca nut extract made an evaluation included organoleptic, thickness, brittleness, water content, pH, and swelling test. The evaluation results of betel nut edible fim extract showed almost the same characteristics as the edible fim that had been circulating in the market which was used as a comparison. The presence of antibacterial activity from edible pinang seed extract is marked by the formation of clear zones. The test results of antibacterial edible activity of areca nut extract films 2.5%, 3% and 3.5% showed the average diameter of clear zone was 14.96 mm, 15.49 mm and 17.05 mm which were included in the strong category.Keywords: Areca catechu Linn, antibacterial, edible film, tannin, streptococcus