Area Of Southern Italy Research Articles

New Mater-Bi, Biodegradable Mulching Film for Strawberry (Fragaria × Ananassa Duch.): Effects on Film Duration, Crop Yields, Qualitative, and Nutraceutical Traits of Fruits.

In the main strawberry areas of Southern Italy, cultivation is carried out by transplanting plants on raised beds (30–40 cm from ground level), mulched with black polyethylene (PE). This technique has becoming increasingly expensive due to the growing prices of plastic mulches, the cost to remove them at the end of crop cycle, and the difficulty to dispose of black, dirty plastic films. The main objective of this research was the replacement of PE mulch with a new biodegradable mulching film Mater-Bi®-based (Novamont), characterized by an increased permanence in the field designed for long crop life. In 2021, two Mater-Bi-based, black, 18 μm thick mulching films were tested under tunnel: N5 as innovative film and N18 as commercial standard film. Black PE film, 50 μm thick was the control. Strawberry cultivars ‘Sabrina’ and ‘Elide’ were cropped on the three mulching films according to a split plot design with four replications. Harvests lasted from March to June 2021. Cvs Sabrina and Elide yielded around 40 t ha−1, while the mean effect of mulching films did not point out differences between the biodegradable mulches and PE. In 4 out of 12 harvests we analyzed samples of fruits to assess the influence of mulches on the contents of °Brix, polyphenols, antioxidant activity, ascorbic acid, flavonoids, and anthocyanins. On average, °Brix was clearly improved in fruits on PE compared to biodegradable films, while all the other qualitative traits resulted in being more dependent on the cultivars and times of sampling effects. Overall, biodegradable mulches are a viable alternative to PE mulch, and the innovative N5 film appeared promising for the enhancement of durability of soil coverage in a long-lasting cycle.

Analysis of small-scale fisheries value chain: An interview-based approach in Italian marine protected areas

Small-scale fisheries in Italy account for around 70% of the total fishing fleet, as well as nearly 30% and 55% of capture-fisheries revenues and employment, respectively. Yet, the sector is experiencing a deep crisis, mostly due to reduced catches and to the consequent decrease in revenues. At the same time, a lack of socio-economic data is hampering an effective resource management and the identification of adequate market solutions to improve fishers’ livelihoods. To address the issue, a survey has been carried out on the value chain’s structure and functioning of small-scale fishery fleets operating inside three marine protected areas in southern Italy. The assessment was performed following an interview-based approach through questionnaires administered to different categories of stakeholders. Results show that small-scale fishers receive the least economic benefits from their catches, while wholesalers and fish shops achieve the highest sale price thanks to their stronger bargaining power. Particularly, the highest mark-ups occur for those product shops that have little or no interest invested in them (i.e., those for which the fishers are paid less). Finally, this paper discusses a few potential market solutions to address the major threats to fisher livelihoods, such as direct sale or transformation of the catch. The latter option should especially be targeted to low value fish, which is often disregarded by shops, but which could generate higher incomes for fishers through the adoption of appropriate marketing strategies.

Vector-borne pathogens of zoonotic concern in hunting dogs of southern Italy

Dogs are commonly exposed to vector-borne pathogens (VBPs), yet few data are available on hunting dogs, which are often at high risk of infection due to their involvement in field activities. To investigate the occurrence of VBPs and evaluate the relative performance of different diagnostic tools, blood and serum samples were collected from hunting dogs (n = 1,433) in rural areas of southern Italy. All samples were tested by Knott's technique for filarioids, serologically (SNAP® 4Dx® Plus) for Anaplasma spp., Borrelia burgdorferi sensu lato, Dirofilaria immitis and Ehrlichia spp. and molecularly (qPCR) for all except B. burgdorferi of the above pathogens plus Babesia spp. and Leishmania infantum. Logistic regression was run to evaluate the statistical associations between the risk of VBP infection and independent variables (such as geographic area of provenience, age class and sex) and K-Cohen formula for assessing the concordance among diagnostic tests. Overall, out of 321 dogs (22.4%) positive to at least one VBP, 28 (1.9%) were infected by filarial species at the Knott's technique. In particular, Acanthocheilonema reconditum was the most prevalent (1.6%), followed by D. immitis (0.2%) and Dirofilaria repens (0.1%). One hundred forty (9.8%) and 231 (16.1%) dogs scored positive to VBPs by serological and molecular methods, respectively. The most prevalent pathogens detected were Ehrlichia spp. (7.3%) with SNAP® 4Dx® Plus, and A. reconditum (7.7%) by qPCR. Statistics revealed a significant association (p < 0.001) between A. reconditum infestation and both Ehrlichia spp. seropositivity and geographical origin of dogs. An agreement of 99.9%, 94.0% and 95.7% for Knott - SNAP® 4Dx® Plus, Knott - qPCR and SNAP® 4Dx® Plus - qPCR for D. immitis was found, respectively. Data demonstrate a high prevalence of VBPs in hunting dogs, indicating that this group of animals is largely exposed to several arthropod vector species and suggesting the transmission risk of pathogens to humans in rural areas of southern Italy. A multi-diagnostic approach and a deeper cooperation among healthcare and stakeholders are required to prevent VBP infections to animals and humans.

PRISMA L1 and L2 Performances within the PRISCAV Project: The Pignola Test Site in Southern Italy

In March 2019, the PRISMA (PRecursore IperSpettrale della Missione Applicativa) hyperspectral satellite was launched by the Italian Space Agency (ASI), and it is currently operational on a global basis. The mission includes the hyperspectral imager PRISMA working in the 400–2500 nm spectral range with 237 bands and a panchromatic (PAN) camera (400–750 nm). This paper presents an evaluation of the PRISMA top-of-atmosphere (TOA) L1 products using different in situ measurements acquired over a fragmented rural area in Southern Italy (Pignola) between October 2019 and July 2021. L1 radiance values were compared with the TOA radiances simulated with a radiative transfer code configured using measurements of the atmospheric profile and the surface spectral characteristics. The L2 reflectance products were also compared with the data obtained by using the ImACor code atmospheric correction tool. A preliminary assessment to identify PRISMA noise characteristics was also conducted. The results showed that: (i) the PRISMA performance, as measured at the Pignola site over different seasons, is characterized by relative mean absolute differences (RMAD) of about 5–7% up to 1800 nm, while a decrease in accuracy was observed in the SWIR; (ii) a coherent noise could be observed in all the analyzed images below the 630th scan line, with a frequency of about 0.3–0.4 cycles/pixel; (iii) the most recent version of the standard reflectance L2 product (i.e., Version 2.05) matched well the reflectance values obtained by using the ImACor atmospheric correction tool. All these preliminary results confirm that PRISMA imagery is suitable for an accurate retrieval of the bio-geochemical variables pertaining to a complex fragmented ecosystem such as that of the Southern Apennines. Further studies are needed to confirm and monitor PRISMA data performance on different land-cover areas and on the Radiometric Calibration Network (RadCalNet) targets.

Epidemiology of HCV and HBV in a High Endemic Area of Southern Italy: Opportunities from the COVID-19 Pandemic-Standardized National Screening or One Tailored to Local Epidemiology?

Simple SummaryEpidemiological data on viral hepatitis are essential to optimize screening programs. For HCV, the Italian Health Ministry planned a cohort screening for those born in 1969–1989. In order to update the epidemiological data of viral hepatitis in a highly endemic area of Southern Italy and assess whether the screening programs currently planned by the Italian government for those born between 1969–1989 will be effective, a retrospective multicenter study was carried out enrolling all COVID-19 hospitalized patients screened for markers of HCV and HBV infection. Indeed, the COVID-19 pandemic has resulted in access to the national health system of an unselected population similar to the general one. Among the 2126 patients evaluated, HBsAg and HCV-Ab prevalence was 1.6% and 5.1%, respectively. For HCV infection, a bimodal distribution was observed, with peaks in the birth cohorts 1930–1939 and 1960–1969 (11.6% and 5.6%, respectively). An analysis of the screening period imposed (born: 1969–1989) demonstrates that only 17% of HCV infection could be captured. Thus, an alignment of the screening period (i.e., birth cohort 1960–1984) would capture 40% of cases. Data show a high endemicity of hepatitis virus in our geographic area and the need for a tailored regional screening program.The COVID-19 pandemic led to the hospitalization of an unselected population with the possibility to evaluate the epidemiology of viral hepatitis. Thus, a retrospective multicenter study was conducted in an area of Southern Italy with the aim of assessing the prevalence of HCV and HBV markers and the ability of current screening program to capture cases. We evaluated 2126 hospitalized patients in seven COVID Centers of Naples and Caserta area in which 70% of the Campania population lives. HBsAg and HCV-Ab prevalence was 1.6% and 5.1%, respectively, with no differences between gender. Decade distribution for birth year shows a bimodal trend of HCV prevalence, with a peak (11.6%) in the decade 1930–1939 and a second peak (5.6%) for those born in 1960–1969. An analysis of the screening period imposed by the Italian government for those born between 1969 and 1989 shows that only 17% of cases of HCV infection could be captured. A small alignment of the screening period, i.e., those born from 1960 to 1984, would capture 40% of cases. The data confirm the high endemicity of our geographical area for hepatitis virus infections and underline the need for a tailored screening program according to the regional epidemiology.

Multidecadal Land-Use Changes and Implications on Soil Protection in the Calore River Basin Landscape (Southern Italy)

In Southern Italy, studies dealing with the analysis of multidecadal land-use changes at the basin scale are scarce. This is an important gap, considering the deep interrelationships between land-use changes, soil erosion, and river dynamics, and hazards at the basin scale and the proneness of Southern Italy to desertification. This study provides a contribution in filling this gap by analyzing the land-use changes occurring in an inner area of Southern Italy, i.e., the Calore River basin, between 1960 and 2018. Working to this aim, we conducted a GIS-aided comparison and analysis of three land-use maps of the study area from 1960, 1990, and 2018, respectively. We analyzed land-use changes at the basin, physiographic unit, and land-use class scale. We also interpreted the results in terms of variations in soil protection against erosion. Most of the detected land-use changes occurred between 1960 and 1990 and mainly consisted of the afforestation of agricultural lands. The latter was mainly concentrated in the alluvial plains and, to a lesser extent, on mountainous reliefs. In contrast, between 1990 and 2018, the land-use remained unchanged in more than 90% of the studied landscape. Artificial surfaces increased by about six times over a period of ~60 years; notwithstanding, they currently occupy about 4% of the basin area. The detected changes led to an overall increase in soil protection against erosion at the basin scale.

Reconstructing Social Relationships in a Post-Lockdown Suburban Area of Southern Italy Using Pastoral Counselling

The growing interest in spirituality has enabled numerous avenues of pastoral counselling support, which can be a useful resource for improving quality of life in the context of significant social deprivation. The aim of this research was to investigate the role of the spiritual dimension of pastoral support interventions created to help the inhabitants of a strongly deprived territory in Southern Italy during the COVID-19 pandemic. Eight people between the ages of 28 and 67 took part in the study. A qualitative research design was applied via online interviews with the participants, who were operators of a pastoral counselling service located on the outskirts of a suburban town. The main emergent themes were the importance of religiosity and spirituality in the lives of the participants, the role that these two aspects play in the lives of those who carry out activities devoted to helping others, and the ways in which these dimensions are used within support programmes responding to the needs of an area characterized by socioeconomic and psychosocial problems. The interviews revealed how pastoral counselling can be useful in situations of stress in highly deprived areas.

Soil microbial biomass, activities and diversity in Southern Italy areas chronically exposed to trace element input from industrial and agricultural activities

High concentrations of trace elements in the soil can have toxic effects on the microbial community which plays a key role in fundamental ecosystem processes like the organic matter decomposition and nutrient cycles. The aim of this study was to evaluate if trace element concentrations in soils chronically exposed to industrial and agricultural activities did affect the soil microbial community, in terms of biomass, activity and diversity. Soil samples differing in trace elements (As, Cd, Co, Cr, Cu, Ni, Pb, V, Zn) concentrations were analysed for the total microbial biomass (Cmic), fungal mycelium, microbial activity, indexes of microbial community structure (fungal percentage of total Cmic) and microbial metabolism (metabolic quotient or qCO2; carbon mineralization rate or CMR), and genetic bacterial diversity (richness, Shannon index and evenness of bacterial groups). Relationships of the microbial variables with individual trace element concentrations, relative Overall Contamination Index (OCI), and ordinary soil properties (pH, water content, organic C content, electrical conductivity) were assessed by simple and multivariate statistical analysis. Neither OCI nor single element concentrations determined a significant reduction in soil microbial biomass, activity and diversity, these variables being mainly driven by soil organic C and water content. Only soil Cr concentration affected indexes of the microbial metabolism and structure, without causing stressful conditions. Even Cmic and bacterial diversity showed higher values in soils richer of Cr. The results suggest that the concentrations of considered trace elements measured in our soils, or probably their bioavailable fractions, did not cause toxic effects on the microbial community. However, the chronic exposure to different patterns of trace element contamination probably elicited site-specific adaptive responses in microbial populations.

Well-Being of Lesbian, Gay, Bisexual Youth: The Influence of Rural and Urban Contexts on the Process of Building Identity and Disclosure.

The study investigates how the territorial community can influence the individual and social well-being of lesbian, gay, bisexual (LGB) youth and especially the recognition of their feelings and the construction of their own identity as well as their needs to be socially recognized. This research focuses on the experiences of 30 LGB individuals (23 males and 7 females), with a mean age of 25.07 years (SD = 4,578), living in urban and rural areas of Southern Italy. Focalized open interviews were conducted, and the Grounded Theory Methodology, supported by the Atlas.ti 8.0 software, was used for data analysis. The textual material was first coded, and then codes were grouped into five macro-categories: Freedom of identity expression in the urban and rural context, identity construction and acceptance process, need of aggregation and identification with the LGB community, role of the interpersonal relationship in the process of identity acceptance, socio-cultural context, and LGB psychological well-being. The results showed a condition common to the two contexts that we can define as “ghettoization.” The young LGB is alone in the rural area due to a lack of places and people to identify with and greater social isolation. On the contrary, although there are more opportunities in the urban area, young people feel stigmatized and ghettoized because “their places” are frequented exclusively by the lesbian, gay, bisexual, transexual, queer (LGBTQ) community. The work will extensively discuss the limitations of the research, future proposals, and the practical implications of the results.

Combining plot measurements and a calibrated RUSLE model to investigate recent changes in soil erosion in upland areas in Southern Italy

Combining plot measurements and a calibrated RUSLE model to investigate recent changes in soil erosion in upland areas in Southern Italy

Green Husk of Walnuts (Juglans regia L.) from Southern Italy as a Valuable Source for the Recovery of Glucans and Pectins

Walnut green husk is an agricultural waste produced during the walnut (Juglans regia L.) harvest, that could be valued as a source of high-value compounds. In this respect, walnut green husks from two areas of Southern Italy (Montalto Uffugo and Zumpano), with different soil conditions, were investigated. Glucans and pectins were isolated from dry walnut husks by carrying out alkaline and acidic extractions, respectively, and then they were characterized by FT-IR, scanning electron microscopy (SEM) and differential scanning calorimetry (DSC). The colorimetric method for the enzymatic measurement of α- and β-glucans was performed. The maximum total glucan yield was recovered from Montalto walnut husks (4.6 ± 0.2 g/100 g DM) with a β-glucan percentage (6.3 ± 0.4) higher than that calculated for Zumpano walnut husks (3.6 ± 0.5). Thermal analysis (DSC) confirmed the higher degree of crystallinity of glucans from Zumpano. The pectin content for Montalto husks was found to be 2.6 times that of Zumpano husks, and the esterification degree was more than 65%. The results suggested that J. regia L. green husks could be a source of glucans and pectins, whose content and morphological and thermal characteristics were influenced by different soil and climate conditions.

Food: Not Only Safety, but Also Sustainability. The Emerging Trend of New Social Consumers

Consumers’ purchasing choices are no longer based only on economic factors but also on ethical reasons related to environmental sustainability and food safety. However, nutritional information on food labels is underused by consumers. Often the lack or incompleteness of information available on the market obstructs the complex transition towards sustainable consumption patterns. This empirical study analysed a sample of 359 consumers from an area in Southern Italy (city of Naples) to identify homogeneous consumer clusters with respect to the assessment of the level of consumer attention to sustainable environmental, social behaviours in daily life, and also to safety attributes. The most important sources of information influencing the consumers’ choices, food safety knowledge, and future purchasing behaviour were analysed. The research sample was self-selected, and the questionnaire for the survey was administrated through a non-probability sample from a reasoned choice. The results indicate that the ideal solution is a five-cluster partition that confirms a good level of attention to intrinsic attributes, in particular food expiry, transparency of food information, food traceability, and seller confidence. In addition, the research could provide an opportunity to consider collaborative actions between policy makers and industries to increase consumer awareness of environmental attributes.

Entomological Surveillance in Former Malaria-endemic Areas of Southern Italy.

Malaria still represents a potential public health issue in Italy, and the presence of former Anopheles vectors and cases imported annually merit continuous surveillance. In areas no longer endemic, the concurrent presence of gametocyte carriers and competent vectors makes re-emergence of local transmission possible, as recently reported in Greece. In October 2017, due to the occurrence of four suspected introduced malaria cases in the province of Taranto (Apulia region), entomological investigations were performed to verify the involvement of local anopheline species. In 2019–2020 entomological surveys were extended to other areas historically prone to malaria between the provinces of Taranto and Matera and the province of Foggia (Gargano Promontory). Resting mosquitoes were collected in animal shelters and human dwellings, larvae were sampled in natural and artificial breeding sites, and specimens were both morphologically and molecularly identified. A total of 2228 mosquitoes were collected, 54.3% of which were anophelines. In all the investigated areas, Anopheles labranchiae was the most widespread species, while Anopheles algeriensis was predominant at the Gargano sites, and Anopheles superpictus and Anopheles plumbeus were recorded in the province of Matera. Our findings showed a potentially high receptivity in the surveyed areas, where the abundance of the two former malaria vectors, An. labranchiae and An. superpictus, is related to environmental and climatic parameters and to anthropic activities.

Adaptive Agricultural Strategies for Facing Water Deficit in Sweet Maize Production: A Case Study of a Semi-Arid Mediterranean Region

Maize is a crucial global commodity, which is used not only for food, but also as an alternative crop in biogas production and as a major energy-supply ingredient in animal diets. However, climate change is jeopardizing current maize production due to its direct impact on weather instability and water availability or its indirect effects on regional climate suitability loss. Hence, new areas for sweet maize cultivation should be considered in the future. Therefore, this study focuses on the possibility of producing maize in a challenging environment in Southern Italy considering rainfed cultivation and two irrigation regimes (full and deficit). The experiment was conducted during two subsequent growing seasons under semi-arid Mediterranean climate conditions. The overall results indicated a significant difference in biomass and yield between irrigated and non-irrigated treatments, and between full and deficit irrigation. Sweet maize cultivated under deficit irrigation gained less biomass than under full irrigation and its development and fruit maturation were delayed. Under deficit irrigation, the plants gave lower yields and a higher percentage of the panicle weight consisted of kernels. Irrigation water productivity was higher for deficit than for full irrigated treatment. These findings indicate the feasibility of sweet maize production in semi-arid areas of Southern Italy using adaptive agricultural strategies including deficit irrigation and controlled water stress. Given the importance of maize production, understanding of maize growth and productivity in a challenging environment may support future agricultural programming and thereby contribute e to mitigation of the direct and indirect effects of climate change.

A combined MaxEnt and GIS‐based methodology to estimate cactus pear biomass distribution: application to an area of southern Italy

AbstractThe need to investigate suitable alternatives to conventional fossil fuels has increased interest in several renewable energy sources, especially in widely available sources of biomass. This has made environmental and socio‐economic improvements possible. In recent years, biogas from biomass has increasingly been considered the most feasible alternative to energy from fossil fuel, as it allows both the possibility to reduce waste disposal treatment and the opportunity to produce green energy. Opuntia ficus‐indica (OFI) has been suggested as a possible biomass as it could represent a suitable resource for producing biomethane as a new frontier within the context of a circular economy. This study aims to define a methodology for evaluating the distribution of OFI biomass that is potentially available for biogas production by applying maximum entropy modeling in a case study of a province located in southern Italy. The geographic information system (GIS)‐based model that was developed made it possible to estimate the suitability of the species in the territory and to define specific indices at the municipal level by enabling the localization of the highest productive territorial areas. Municipalities were grouped into three different classes ranging from low to high potential ones. In future years, new municipalities could be part of the high‐potential group, due to global warming resulting from climate change. The results could be relevant to the intervention priorities established by the European Union related to the planning activities supported by the European Structural and Investment Funds within the Smart Specialization Strategy. In this regard, knowledge of the potential production and distribution of a species in a territory is highly valuable information for the quantification of the biomass potentially available for biogas production, prior to strategic planning for the improvement of the biogas sector. © 2021 Society of Chemical Industry and John Wiley &amp; Sons, Ltd

Measuring Risk Perception in Pregnant Women in Heavily Polluted Areas: A New Methodological Approach from the NEHO Birth Cohort.

Risk perception (RP) evaluation during pregnancy and its relationship with lifestyles are considered useful tools for understanding communities living in high-risk areas and preventing dangerous exposure. It is well known that exposure to pollutants and less-healthy lifestyles may result in increased disease occurrence during life. Our work investigated environmental RP through ad hoc questionnaires administered to 611 mothers within the NEHO birth cohort, recruited in three heavily contaminated areas of Southern Italy. Four different RP indices, an exploratory factorial analysis (EFA), and a latent class analysis were evaluated from questionnaires. The highest values of risk perception index were observed in the Milazzo site (0.64 ± 0.16) and the lowest in the Crotone site (0.5 ± 0.18). EFA revealed four latent factors, including different items describing environmental pollution, and subjects were classified into four latent classes with different RP indices. Significant RP profiles were different among the sites (p < 0.001). Our results did not demonstrate any association between RP and lifestyles during pregnancy. Improving healthy lifestyle behaviours, particularly in polluted areas, would generate co-benefits by preventing further risk factors. As remediation interventions can take a long time, it needs to improve healthy lifestyles in residents until remediation is completed.

Associated screening for HCV and SARS-Cov2 infection in an urban area of Southern Italy: A cohort study.

The SARS-Cov2 pandemic led to reorganize the clinical activities in Italy, reducing the available resources to manage other diseases. In particular,important milestones achieved towards HCV elimination are now at risk of being nullified. Moreover, the burden of SARS-Cov2 asymptomatic carriers remains still an unresolved issue. To associate those two screenings, in order to respond to these clinical needs seems of actual interest. A prospective cohort study was set-up in an urban area of the Naples province, in which a contemporary screening for HCVAb and SARS-Cov-2-Ab rapid blood tests was performed. Of the 3556 eligible subjects,2740(77.05%) participated.Of them,1.4% resulted SARS-Cov2-positive, none resulting positive for SARS-Cov2-RNA, whereas 1.5% were HCVAb-positive and 0.18% HCVRNA-positive. Of those HCVAb positive,87.8% were already aware and treated in 88.8% of cases .Of the other 4 patients aware of their positivity,2(50%) were HCVRNA-positive. Of the 5 HCVAb-positive unaware of their positivity, 2 were HCVRNA-positive. HCVAb-positivity was only detected in patients of >41 years. The screening in an urban area of Southern Italy showed a seroprevalence of SARS-Cov2-Ab and HCV-Ab of 1.4% and 1.5%, respectively,whereas 0.18% had an active HCV infection. This demonstrates how the pandemic can be an opportunity to promote prevention activities for HCV.

Fruit Yield, Polyphenols, and Carotenoids in Long Shelf-Life Tomatoes in Response to Drought Stress and Rewatering

The Mediterranean long shelf-life tomato (or long storage tomato) is a plant traditionally cultivated under no irrigation in the Southern regions of Italy, whose fruits have typical high sensory and nutritional quality. However, yield levels are kept low under current cultivation conditions. In this study, the effects of repeated cycles of drying and rehydration on crop productivity and nutritional quality of fruits in terms of polyphenols and carotenoids content were assessed in long shelf-life tomatoes cultivated in a typical semi-arid area of Southern Italy. The three local Sicilian landraces ‘Custonaci’, ‘Salina’ and ‘Vulcano’, and the commercial tomato hybrid ‘Faino’ (control) were submitted to three irrigation treatments: DRY (no irrigation); IRR (long-season full irrigation); REW (drought/rewatering cycles). Total 450, 4710, and 1849 m3 ha−1, were distributed in DRY, IRR, and REW, respectively. At harvest, fruit yield, polyphenols (as total, flavonoids, and hydroxycinnamoyl quinic acids-HCQA), and carotenoids contents (lycopene and β-carotene) were measured. All cultivars benefitted from very limited irrigation in REW, raising their productivity (up to +147% in ‘Vulcano’) with respect to that of plants overstressed in DRY. Irrigation water use efficiency in REW was higher than that in IRR. Water shortage in REW led to a polyphenols content of fruits that was slightly lower (171.1 μg g−1) than that in DRY, but higher than that in IRR (116.8 μg g−1). All local landraces had greater contents (&gt;160 μg g−1) than control (113.0 μg g−1). Under REW and DRY, the two landraces ‘Salina’ and ‘Vulcano’ produced fruits with the same polyphenols and flavonoids contents. Overall, the two water stressed treatments (DRY and REW) did not differ for HCQA content (&gt;66 μg g−1), which was significantly higher than that in the irrigated plots (&lt;50.55 μg g−1). Lycopene content in DRY and REW exceeded 100 μg g−1; among local types, ‘Vulcano’ exhibited the highest content (106.7 μg g−1). The β-carotene content did not change in ‘Custonaci’ and ‘Salina’, whereas in ‘Vulcano’ it was greater in DRY and REW. Only in ‘Faino’ water limitation in REW exerted a clear promoting effect on β-carotene content of fruits. The results of the present study reveal how rewatering after long-lasting drought periods is beneficial to long shelf-life tomatoes in terms of productivity. Appropriate drought cycles in REW may also help the fruits to retain high nutritional levels.

Air Quality Assessment of a School in an Industrialized Area of Southern Italy

In this study, simultaneous monitoring of indoor and outdoor Volatile Organic Compounds (VOCs) was carried out in a school, by both Radiello® cartridges and real-time monitors (Corvus, IonScience Ltd., Fowlmere UK). Moreover, an outdoor air quality assessment was performed with data from an air quality monitoring station (ARPA Puglia) located close to the school. In particular, VOCs, Benzene, Toluene, Ethylbenzene, and Xilenes concentrations, obtained by using Radiello® diffusive samplers, were monitored in two classrooms, two bathrooms, and outside of the school building for three weeks during winter 2019. Simultaneously, the Total VOC (TVOC) concentrations were measured by means of real-time monitors inside and outside the classroom in order to individuate the activation of sources during the sampling campaign days. The results evidence that indoor TVOC concentrations were bigger than those outdoors; this suggests the presence of indoor pollutant sources, both in the classrooms and the bathrooms. The results of our study can help the school management by the following recommendations: increasing ventilation in classrooms during school activities and after cleaning; involving students in discussions regarding the use of acrylic paints, permanent markers, perfumes and deodorants, and the limitation of smoking activities both in bathrooms and outdoor spaces.

Hepatitis E Virus seroprevalence among cows in a rural area of southern Italy

Hepatitis E virus (HEV) is considered to be an emerging zoonotic disease, which causes numerous cases of hepatitis and deaths worldwide each year. Pigs are a host reservoir of HEV, but numerous other wild and domestic species can be infected. The aim of this study was to carry out serological screening of anti-HEV antibodies in cattle in the Sicily region (Southern Italy). Between April and December 2018, 231 serum samples were collected from cows and analysed with enzyme-linked immunosorbent assay (ELISA), for detection of HEV antibodies (IgG). The overall prevalence of anti-HEV antibodies was 36.36% (84/231; 95% CI 30-43). There were statistically significant differences in the HEV seroprevalence in animals of different ages (&lt;11 months: 11.41%; &gt;12 months: 39.30%; &gt;24 months: 54.10%) (P&lt;0.05). No statistically significant differences were detected between genders (31.37% in male and 37.38% in female) (P &gt;0.05). This study shows that further investigation of HEV in cattle is required to understand better the epidemiology in farm animals and the potential zoonotic risks for humans. This is the first report of HEV seroprevalence for cows in Italy, which provides baseline data for further studies and for control of HEV infection in cattle.