Background: Raised serum sodium, low levels of serum calcium and increased serum glucose has been implicated in the development of cataract. Our study compares the aqueous levels of sodium, calcium and glucose among patients with different stages of cataract. Aims and Objective: - To compare the aqueous levels of sodium, calcium and glucose among patients with different stages of cataract – Immature cataract, Mature cataract and Nuclear Cataract. - To compare the serum levels of sodium, calcium and glucose among patients with different stages of cataract – Immature cataract, Mature cataract and Nuclear Cataract. Methods : Patients were divided into 3 groups. Group 1 –Immature cataract, Group 2- Mature Cataract, Group 3- Nuclear Cataract. Serum and Aqueous humour was collected intra-operatively and was sent for biochemical analysis Results: In immature cataracts: -The sodium concentration in aqueous humour was slightly higher than normal ie, 147.8 mEq/L. -The calcium level were also in normal limits was 5.6 mg/dl. -The mean glucose concentration in aqueous humour was 60.4 mg/dl which was higher than the normal limits. In mature cataracts: The mean aqueous concentration of sodium was higher than normal limits. The mean aqueous concentration of calcium levels were also in normal limits. But the glucose level was less than normal. In nuclear cataracts: -The mean aqueous concentration of sodium and calcium was in normal limits. -The glucose level were much higher than normal-86.5 mg/dl. Conclusion: - With respect to aqueous, the level of sodium and glucose were higher than normal in immature cataract and the mean aqueous concentration of calcium was higher in mature cataract. -The mean aqueous levels of sodium calcium and glucose were all raised in nuclear cataract.