Aqueous Formalin Solution Research Articles

A histological pattern of the internal genitalia of cows with follicular ovarian cyst

Intensification of the cattle population reproduction is an indispensable condition for the increase in the production of cattle breeding products. However, everyday abnormal effects of various factors on the intensively functioning organism of animals lead to the emergence of pathologies that restrain the rate of increase in the number of cows. Despite a wide range of problems, the results of numerous studies confirm and emphasize that a significant cause of symptomatic infertility in rejected cows is ovarian follicular cysts, accompanied with pathological changes in other organs of the reproductive system. Transrectal and ultrasound examination allows to diagnose pathology, but the issue of changes at the microscopic level in the ovaries and other organs of the reproductive system remains insufficiently studied, which inhibits the development of new methods of restoring the endocrine function of the gonads. In this regard, the morphology and pathology of the ovaries and uterus of cows require further careful study. The research was conducted in the conditions of the agricultural production cooperative “Agrofirma Semenivska”, Sumy region, on Holstein cows of different ages and productivity. At the gynecological stage of dispensation, the condition of the internal genital organs of 47 infertile cows, 14 of which were rejected, and 8 diagnosed with ovarian follicular cyst, was examined. In the conditions of the meat processing plant, the uterus and ovaries were dissected from the genitals taken immediately after slaughtering 8 cows. Pieces of tissue measuring 1 x 2 cm were cut from the walls of the uterus, fallopian tubes, and ovaries, then fixed in 10 % aqueous formalin solution; histosections were made according to the generally accepted method, being stained with hematoxylin-eosin, and examined by light microscopy. It has been established that on the histopreparations, made from the ovary with a follicular cyst, there is desquamation of individual cells of the covering epithelium, atresia of the obliterating type, which is characterized by dystrophic changes of granulosis with pronounced desquamation of follicular cells filling the cavity of the cystic follicle with the subsequent formation of a fibrous atretic body. While examining histological sections from the uterus, a large number of arterial vessels with thick walls surrounded by connective tissue fibers and groups of endometrial glands of a typical structure, which is typical for endometriosis, have been found in the thickness of the myometrium. The endometrium has turned out to be represented by a cytogenic stroma, the density of the cells of which gradually decreases from the surface to the muscle layer, in the basal layer, the stroma is almost entirely replaced by connective tissue. Behind the follicular cyst of the ovary, traces of mucus have been found in the lumen of the fallopian tube.

Pathomorphological changes in German boxer dog infected with Babesia canis parasites. Clinical case

The article covers the results of a comprehensive pathomorphological study of Babesia canis parasitic infestation in a dog of the German boxer breed. In April 2020, a male German boxer with pronounced clinical signs of anorexia, remitting fever, hematuria, and manifestations of general jaundice was admitted to the Dr. Markevych clinic (Lviv). The anamnesis evidenced that after daily walks in the park of Lychakiv district (Lviv) the pet’s owner had been detecting 3–5 Ixodes ticks on the dog’s body for several days in a row. Cytological examination of blood smears stained by the Romanovsky–Giemsa method revealed a massive lesion of erythrocytes by the Babesia canis parasite. The applied treatment strategy, namely the introduction of the drug with the active substance imidocarb dipropionate, was not successful. The dog died in the clinic. An autopsy was performed in agreement with the pet owner. Macroscopic examination revealed foamy fluid in the trachea, pulmonary edema and lobar pneumonia, massive diffuse hemorrhages on the epicardium and costal pleura, splenomegaly, serous lymphadenitis, acute venous stasis, hepatic and renal dystrophy, and pancreonecrosis. General anemia and transudate accumulation in serous cavities were established. Tissue fragments of lungs, kidneys, liver, spleen, and pancreas were selected for a histopathological examination and fixed in 10 % neutral aqueous formalin solution, followed by dehydration in alcohols, filling into paraffin blocks and subsequent production of histopreparations. Staining was performed with eosin and Ehrlich’s hematoxylin according to the conventional methods. Microscopically, the lungs showed the alveoli swelling, thickening of the alveoli walls with blood overflow, bronchioles’ inflammatory infiltration with the accumulation of desquamated epithelium and exudate in the lumens of large bronchi. Extracapillary glomerulonephritis and hyaline-drop renal tubular dystrophy were observed in the kidneys. Vascular endothelium edema, granular hepatocyte dystrophy, sharp dilation of central vein and intraparticle and interparticle capillaries were found in the liver. Significant hyperplasia was observed in the red pulp of the spleen. Reticuloendothelial proliferation, erythrocyte proliferation with a diffuse accumulation of hemosiderophages due to increased erythrocyte disintegration, sinus dilatation, and atrophy of white pulp lymph nodes were also revealed.

Comparative morphology of the paw pad of the main arboreal Xenarthras from Amazon.

Paw pads are specializations of the integument and important shock absorbers of the locomotor system, as well as pressure, pain, temperature, storage and excretion sensors. Aiming to describe the paw pad morphology of the main arboreal xenarthras species in the Amazon, 16 animals were studied, Bradypus variegatus (6), Choloepus didactylus (5), Tamandua tetradactyla (3) and Cyclopes didactylus (2) that after death were donated to the Animal Morphological Research Laboratory (LaPMA / Ufra). The corpses were thawed and fixed with 10% aqueous formalin solution. The paw pads were measured, photographed and removed by skin incision dorsally to them. Fragments were used for routine histological processing, using two staining techniques: Hematoxylin-Eosin (HE) and Gomori's Trichrome, in sections of 6 to 8µm. Choloepus didactylus, Tamandua tetradactyla and Cyclopes didactylus have digital paw pads, one in each digit, and one palm, as well as a plantar, whose shapes and colours are distinct from each other. Bradypus variegatus, however, has only one palmar and one plantar pad. Histologically, they have keratinized stratified squamous epithelium, supported by a large amount of collagen fibres and fibroblast cords in the dermis and hypodermis. Groups of eccrine sweat glands were observed in the reticular dermis of C.didactylus, B.variegatus, T.tetradactyla and only in the hypodermis of Cyclopes didactylus.

ГІСТОЛОГІЧНИЙ АНАЛІЗ ЛІМФОЇДНИХ УТВОРЕНЬ ШЛУНКА І ТОНКОЇ КИШКИ У КЛІНІКО-ПАТОГЕНЕТИЧНОМУ ПРОФІЛІ БАБЕЗІОЗУ

Peripheral lymphoid organs respond immediately to a decrease in the body's defence responses, and their localisation and morphological specificity account for their active involvement in immunogenesis (especially in animals). This fact accentuates the morpho-functional estimation of the lymphoid tissue condition with the purpose of timely diagnostics of starting deviations and preventing the development of decompensated changes. Thus, the analysis of histological changes of the latter in natural and experimental prototypes of babesiosis is relevant. The aim of the research is to define the role of histological changes of lymphoid masses in the stomach and small intestine in the formation of the clinical and pathogenetic profile of babesiosis. We studied the structural changes of lymphoid masses of the stomach and small intestine in animals with babesiosis postmortem by histological methods. Fixation was traditionally carried out in 12% aqueous formalin solution, dehydration in alcohol (30º to absolute), and pouring (paraffin/ celloidin). Staining was conducted with eosin and haematoxylin, Van Gizon, Brasche. Analysis was performed using an Olympus BX-41 microscope, Japan (x 100; x 200; x 1350). Verification was carried out by cytological, ultramicroscopic, molecular genetic methods (polymerase chain reaction) and in a biological experiment on animals. Analysis of specimens of the lamina propria in the gastric mucosa of the animals in the experimental group and clinical observation group established that the latter contained diffuse accumulations of the lymphoid tissue. Their dispersal involved different parts of the organ, including quite large areas. Haematoxylin and eosin staining gave a clear histological pattern with a marked contrast of fragments, which contributed to diagnostic resources. Lymphoid follicles were observed occasionally, their marginal, mantle zones were blurred. The germinative centres were not detected and did not show any lucency. Neutrophilic granulocytes, monocytes and plasma cells predominated among the cellular leucocyte populations. Examination of the lymphoid apparatus of the small intestine showed predominantly diffuse nodular hyperplasia, which was widespread. A focal version of the nosological prototype was observed in individual specimens occasionally, presented as single cells (most often in the terminal parts of the organ). Histological changes of lymphoid masses of the stomach and small intestine in animals with babesiosis consisted in the development of expressive diffuse nodular hyperplasia with disorganization of the structure of lymphoid follicles, destruction of zonality of the latter, making it impossible to detect germinative centres.

THE ROLE AND SIGNIFICANCE OF MORPHOLOGICAL CHANGES IN INTRAORGAN VESSELS IN ANIMALS DURING PATHOGENESIS OF BABESIOSIS

The aim of this study is to investigate morphological changes in intraorgan vessels and their role in susceptible animals in the pathogenesis of babesiosis. The material of the study included the intraorgan vessels of domestic dogs with clinically and laboratory confirmed babesiosis and wild mouse-like rodents from latent foci of babesiosis in Volyn, Zhitomir, Kiev, Poltava, Sumy, Kharkov, Chernigov regions. For histological study, we used standard fixation in a 12% aqueous formalin solution in phosphate buffer (pH = 7.0-7.2). After the post-fixation and dehydration, the samples were placed into paraffin blocks; then a series of histological slices (5 μm) were prepared. The preparations were stained with hematoxylin and eosin, according to Van Gieson technique. All test animals, regardless of the species taxonomy, were divided into two groups. The control group included clinically healthy animals (∑ = 36). The group under clinical study consisted of animals with clinically and laboratory confirmed babesiosis (∑ = 91). Histological changes in intraorgan vessels in the control group corresponded to the anatomical and functional parameters in the health. The preparations taken from the group with basesiosis demonstrate that microvessels are branched, different in size, spasmodic; their walls have signs of delamination and through lesions. Delamination developed gradually, started with the detachment of a small horizontal fragment of the outer layer. Defects in the integrity of microvessels resulted in penetrations; there was an active diapedesis of erythrocytes into the periovascular space. The accumulation of erythrocytes directed to the areas of connective tissue, branching of blood vessels, and fatty fragments. Hemorrhages were local in nature, concentrated in the form of small blurred foci of irregular shape. In the pathogenesis of babesiosis, the defects in the walls of blood vessels with their further penetration and dissection, hemorrhages, microvesiculation of the endothelium, and its desquamation play a critical role. Other manifestations included the development of intravascular blood coagulation, thrombosis, stasis, microcirculation disorders followed by ischemia, destructive and degenerative changes in the walls of blood vessels.

Synthesis and Antioxidant Activity of N-Aminomethyl Derivatives of Ethosuximide and Pufemide Anticonvulsants

Aminomethylation of 3-methyl-3-ethylpyrrolidin-2,5-dione (ethosuximide) and 3-(4-isopropoxyphenyl)pyrrolidine-2,5-dione (pufemide) with a 25% aqueous formalin solution and alkyl(aryl)amines yielded corresponding N-aminomethyl derivatives. The antioxidant activity of the synthesized compounds and their effect on some parameters of the blood coagulation system were studied.

Anatomy of facial nerve in maned wolf (Chrysocyon brachyurus - Illiger, 1815)

Abstract: Maned Wolf (Chrysocyon brachyurus) is considered one of the largest and most beautiful canids in South America, inhabits the Cerrado biome, and presents nocturnal habits, especially during the twilight period. It is in danger of extinction, mainly due to anthropic activity in its habitat. What certainly raises the importance of studying and better understanding its biology. The mammalian Facial Nerve is the seventh cranial pair and controls the functions of facial expression muscles and others. Once the anatomical descriptions of this canid are scarce, this paper aimed to describe the anatomy of Facial n. in Maned Wolf and correlate it with that of domestic canids, a phylogenetically close species whose anatomy is well known. For this research, four adult specimens were used, whose carcasses were collected along highways in southeastern Goiás State - Brazil (SISBIO 37072-2), or donated by the Wildlife Screening Center (CETAS) in Catalão - Goiás State. The specimens were fixed in a 10% aqueous formalin solution and stored in the same solution. Dissection was performed by macroscopic anatomical methods. The research was carried out with a favorable opinion of the Animal Use Ethics Committee (CEUA) of the Federal University of Uberlândia (UFU) (under n° 067/12). Anatomical preparations of post-mortem Maned Wolf did not reveal the presence of Caudal Auricular, Internal Auricular, nor Stylohyoid n.. Small ramifications of Facial n. can be considered as collateral branches. The Facial n. emerged as a single trunk, Facial n. Trunk, which branches into Auriculopalpebral, Dorsal Buccal, and Ventral Buccal n..

Pathomorphological changes in the testes of males with babesiosis

The aim of the work was to study pathomorphological changes in the testes in males with babesiosis. Diagnosis of babesiosis in sick males was performed based on the detection of typical clinical signs and blood smears. Blood smears were fixed in methanol and stained according to the Romanovsky-Giemsa method with hematoxylin and eosin. For histological studies, pieces of testicles were selected, which were fixed in an 8% neutral aqueous formalin solution. The prepared material was poured into paraffin according to the conventional method. Histological sections were stained with hematoxylin and eosin using the method of Van Gieson and Mallory. Pathomorphological examination of stained histological sections revealed changes characteristic of parenchymal orchitis. The spermatogenic epithelium of the convoluted seminal tubules was drained down in all fields of vision. The lumen of the tubules was filled with protein detritus, exfoliated cells of the spermatogenic epithelium and macrophages. Part of the Sertoli cells was in a state of vacuolar degeneration. Focal clusters of lymphoid-macrophage infiltrates were visible between the convoluted seminal tubules, especially multiple ones around the blood vessels. Endocrine cells were subjected to atrophy due to compression by cell infiltrates and connective tissue growths.

Pathomorphological changes in the testicles of goats in acute anaplasmosis

The aim of the research was to study pathomorphological changes in the testicles of goats in acute anaplasmosis. Diagnosis of anaplasmosis in sick goats was carried out taking into account the characteristic clinical signs and studies of peripheral blood smears. Blood smears after fixation in alcohol ether were stained according to Romanovsky-Giemsa by hematoxilin and eosin. For histological examination, pieces of testicles and appendages of testicles of 0.5 cm thick were selected, which were fixed in an 8% neutral aqueous solution of formalin. The resulting material was poured into paraffin according to the generally accepted method. Histological sections of 5-8 microns thick were made from the obtained paraffin blocks and stained with hematoxilin and eosin using the method of Van Gieson and Mallory. Micro photos were made with a series of rendered images on the basis of Olimpus 2000. The study revealed the following changes: the spermatogenic epithelium has desquamation, in the lumen of the convoluted seminiferous, there were tubules protein detritus, exfoliated cells of seminiferous epithelium and macrophages. Sertolli cells were in a state of vacuole dystrophy and necrosis. In the interstitial tissue around arterial vessels and convoluted seminiferous tubules, focal accumulation of lymphoid-macrophage infiltration was found. Endocrine cells located in the interstitial were in a state of vacuole dystrophy and necrosis. Thus, in goats older than 6 months with acute anaplasmosis in the testicles, pathomorphological changes are characteristic of acute parenchymal orchitis.

Antioxidant properties of hidazepam and amitriptyline with pain syndrome of rats

Peroxide modification of cell biostructures and functions due to the activation of free radical oxidative processes underlies the pathogenesis of a significant number of diseases different in its etiology. The ability of pharmacological drugs which have antioxidant properties inhibit free radical peroxide oxidation and stabilize the structure and function of cell membranes and thus create optimal conditions for the preservation of homeostasis of cells and tissues. It makes pathogenetically grounded their widespread using in medical practice. Therefore, it was expedient to study the effect on the state of lipid peroxidation in the case of the development of the pain syndrome of drugs which are used during anesthesia as an adjuvant.
 For the study, drugs from the group of psychotropic drugs were selected as adjuvants. This is due to that pain syndromes of the different origin often cause psycho-emotional disorders. In addition, pain is not only a symptom of many acute and chronic diseases but also a complex psycho-physiological phenomenon which involves the mechanisms of the regulation and the formation of emotions and motor, humoral and hemodynamic reactions.
 The use of antidepressants in combination with other analgesics pharmaceuticals proved to be effective in placebo-controlled trials in the patients with the chronic pain syndrome. Among the drugs from the group of tranquilizers the greatest application in our time have been acquired derivatives of benzodiazepine. They are recommended for use on the background of analgesics in the case of the treatment of chronic pain syndrome in order to reduce effects of the excitement, anxiety, muscle tension, as effective and safe sleeping pills.
 So the purpose of the work is to study the effects of hidasepam and amitriptyline with the prophylactic and therapeutic administration on the intensity of the processes of the lipid peroxidation in serum, erythrocyte membranes, homogenate of rat spinal cord in case of the development of pain syndrome.
 The results of the research indicate that the development of pain syndrome caused by subcutaneous administration in the dorsal surface of the foot of the right posterior paw of the animal 0,1 ml 2% aqueous solution of formalin is accompanied by the appearance of oxidative stress in the experimental animals. In blood serum, erythrocyte membranes, spinal cord homogenates, the content of TBC-active products of the lipid peroxidation increases. Prophylactic-therapeutic administration of hidazepam per os in a dose 0,02 mg/kg of the body weight of animals prevents the development of oxidative stress in the investigated biological substances thus protecting them from damage and reducing the intensity of the pathological process. Pprophylactic and therapeutic administration of amitriptyline per os in a dose 16 mg/kg of the animals weight does not affect the intensity of the lipid peroxidation processes. The obtained results allow to optimize the treatment regimens due to the combined using of the non-narcotic analgesics and the non-steroidal anti-inflammatory drugs with the adjuvant anti-oxidant drugs from the group of the psychotropic medicines.

Мікроскопічні зміни в тонкій кишці котів за інфекційного перитоніту

FIP is widespread throughout the world. It has been found that about 25% of cats living alone at home and 75-90% of cats living in groups are seropositive to this coronavirus (Cave, Golder, Simpson, & Addie, 2004; Pesteanu-Somogyi, Radzai, & Pressler, 2008). However, morphological changes at the FIP are not fully described. The purpose of the work was the detail investigation of microscopic changes in the small intestine of cats at the infectious peritonitis. The task of the study was to establish the microscopic changes in the mucosa, submucosa, muscular layer and serosa of the various parts of small intestine at the infectious peritonitis. We conducted a dissection of 26 cats of different breeds and age, which died because of infectious peritonitis. In carrying out a dissection for histological research fragments from different parts of the small intestine (middle part of the duodenal, cranial, middle and caudal third of the jejunum, cranial and caudal halves of the ileum) were selected. Selected fragments were fixed in 10% neutral (pH 7.2) aqueous formalin solution, dehydrated in ethanol of increasing concentration (600, 700, 800, 960, 1000) and was embedded in paraffin. Slides with the thickness of 7 - 10 micrometers were painted by hematoxylin and eosin. In dissection of 19 cats we established a mixed form of infectious peritonitis (73.1%), and in 7 cats – a dry form of this disease (26.9%). At the histological examination of the small intestine of cats which died because of infectious peritonitis we have found that microscopic changes in mixed and dry forms of the disease in different parts of the small intestine were similar. The most expressive microscopic changes are found in the serosa, and the least expressive – in the mucosa. The gradual decrease in the degree of microscopic changes in the direction from the serosa to the mucosa shows that such changes were caused by a factor, which acts from the abdominal cavity. On isolated sites of small intestine, the destruction of mesothelial cells was established, and for many sites of small intestine the pronounced metaplasia of these cells was established. These cells turned into columnar epithelial cells, which formed quite large cellular fields on the outer surface of the small intestine. At the same time, the cells themselves increased significantly in size. The long axis of the columnar mesothelial cells was oriented perpendicularly to the outer surface of the wall of the small intestine. The cytoplasm of modified mesothelial cells was distinctly basophilic. The nuclei had an oval, wrong or rounded shape. In some cases, there was a discomplexation of adjacent modified mesothelial cells. They lost contact with each other and between them there were quite significant intervals. In most cases, the close contact of modified mesothelial cells with the basal membrane was preserved; however, some cells partially lost contact with the basal membrane. Apical cytoplasm (turned to the abdominal cavity) of the part of modified mesothelial cells formed a brush border. In other layers of the wall of the small intestine microscopically mainly disorders of blood circulation, edema and infiltration with lymphocytes and less monocytes are established.

Гістоструктурні показники перешийку яйцепроводу гусок у продуктивному періоді першого циклу яйцекладки

The oviducts of the geese of large grey breed of 8 - 13 months of age were investigated. The macroscopic parameters of the organ were determined in each group. For histological studies, the samples of oviduct isthmus were fixed in a 10% aqueous formalin solution. The histological slides were staining by the following methods: hematoxylin and eosin, tricolor method (by Mallory), methyl green-pyronin (by Brashe), PAS-reaction and alcian blue. It was established that the period of egg-laying in the goose of a large grey breed began at the age of 10 months with the peak of egg-laying at the 11th - 12th months of age. Oviducts of geese of the 8th and 9th month of age are not macroscopically differentiated into divisions. Structural features of the wall of the oyiduct isthmus were determined at the microscopic level. The thickening of the isthmus wall occurred proportionately to the mucous membrane and muscle membranes. The beginning of the formation of the secretory apparatus of the isthmus came from the age of 8 to 9 months of the geese. Since the age of 10 months, the parameters of the thickness of the mucous membrane and the height of folds have not veraciously changed. It is a feature of the completion of the relief formation of mucous membrane and the secretory apparatus. There was a maximum increasing of the diameter of the glands of the mucous membrane for geese of this age group. It is an indicator of the completion of the differentiation of the glandular apparatus of the isthmus at the time of the start of the egg-laying. The morphometric indices of lumen epithelium and glands decreased by almost 1.4 times when egg, surrounded by membranes, is presented in the isthmus. It is caused by the extrusion of the secret and the formation of the material of the subshell membranes. The height of the folds of the mucous membrane and the diameter of the tubular glands in the geese of 13 months of age did not veraciously change, with a decreasing of egg productivity on 35%. Wherein, the thickness of the mucous membrane even increased. Such dynamics of morphometric indices is the result of accumulation and stagnation of the secret in the cytoplasm of glandular cells.

New Alternative Methods to Teach Surgical Techniques for Veterinary Medicine Students despite the Absence of Living Animals. Is that an Academic Paradox?

Due to a raised ethical mentality, veterinary schools are pursuing methods to preserve animal corpses used for surgical technique classes in an attempt to reduce the use of living animals for teaching. Generally speaking, animal and human bodies are usually preserved with 10% aqueous formalin solution especially for descriptive anatomy classes. Other possibilities include the use of glycerol, alcohol and phenol. At present, new fixatives have been developed to allow a better and longer preservation of animal corpses in order to maintain organoleptic characteristics, i.e. colour, texture, as close as possible to what students will deal with living animals. From 2004, in our college, surgical technique classes no longer use living animals for students' training. Instead, canine corpses chemically preserved with modified Larssen (MLS) and Laskowski (LS) solutions are preferred. The purpose of this study was to investigate comparatively the biological quality of preservation of these two solutions and to evaluate students' learning and acceptance of this new teaching method. Although these fixatives maintain body flexibility, LS solution failed to keep an ordinary tissue colouration (cadavers were intensely red) and tissue preservation was not adequate. By contrast, MLS solution, however, did not alter the colouration of cadavers which was fairly similar to that normally found in living animals. A remarkable characteristic was a very strong and unpleasant sugary odour in LS-preserved animals and therefore the MLS solution was the elected method to preserve cadavers for surgical technique classes. The students' feedback to the use of Larssen-preserved cadavers was very satisfactory, i.e. 96.6% of students were in favour of the use of cadavers for surgical training and on average 91.8% (2002-2003) of students preferred the MLS solution as the chemical preserver, whereas only 8.2% elected LS solution for teaching purposes. From the students' point of view (95.1%) the ideal class would be an initial training in MLS cadavers followed by classes with animals admitted to the Veterinary Hospital.

Aldol Synthesis with an Aqueous Solution of Formalin.

AbstractFor Abstract see ChemInform Abstract in Full Text.

Isolation of Polysaccharides from the Callus Culture of Lemna minor L.

Two fractions that included acid arabinogalactan and pectin were extracted from the callus culture of duckweed plants (Lemna minor L.) with water and ammonium oxalate. Residues of galactose and arabinose in the 2.0-2.5:1 ratio were the major constituents of acid arabinogalactan. The pectin fraction contained primarily residues of glucuronic acids, galactose, and arabinose. The percentage of arabinogalactan and pectin was similar. The yield of polysaccharide fractions did not depend on the method for their isolation. Extraction with water, treatment of the biomass with an aqueous solution of formalin and diluted hydrochloric acid, and extraction with an aqueous solution of ammonium oxalate allowed us to obtain the highest-purity pectin polysaccharide.

Aldol Synthesis with an Aqueous Solution of Formalin

With an aqueous solution of formalin, aldol reaction of trimethylsilyl enol ether proceeds moderately using tetrabutyl­ammonium fluoride (TBAF). Furthermore, catalytic asymmetric hydroxymethylation with trimethoxysilyl enol ether using water tolerant (R)-BINAP-AgOTf as Lewis acid and KF as Lewis base has been achieved in aqueous media.

Estudo anatômico dos gânglios celiaco, celiacomesentérico e mesentérico cranial e de suas conexões em fetos de búfalos sem raça definida (Bubalus bubalis, Linnaeus - 1758)

We studied the morphology of the celiac, celiacmesenteric and cranial mesenteric ganglia. These ganglia are responsible by the gastrointestinal motility, being so its anatomotopographical characterization necessary for comparative anatomofunctional purposes. Thirty mixed breed buffalo fetuses aged 4 to 10 months were injected with Neoprene Latex 650 into the thoracic aorta and shortly after fixed in 10% aqueous formalin solution for at least 72 hours. The plexus exhibited 9 celiac ganglionar nodes, 5 of which on the right and 4 on the left side. The cranial mesenteric node occurred in 2 samples, where appeared both in a right and a left portion, in a semilunar disposition. On the other hand, 30 cranial celiac mesenteric ganglionar nodes were observed. They showed different morphological arrangements: single node with right and left portions occurred in 21 samples (70%), independent nodes (13.3%), left celiac mesenteric ganglionar node with a right mesenteric portion (10%), a right celiac mesenteric ganglionar node with a left mesenteric portion (6.7%). In regard to the shape of the cranial celiac mesenteric ganglionar node, we noticed the prevalence of the semilunar type (86.7%). The larger and smaller splanchnic nerves as well as the lumbar splanchnic nerves are considered connection structures of these ganglia. Celiac branches stemmed from the dorsal vagal trunk and in 21 samples they stemmed from the ventral vagal trunk. Microscopic evaluation allowed us the presence of nodular ganglionar tissue, which showed differences characteristics. This tissue was involved by a capsule, made up elastic, reticular and collagen fibers.

Bakteriologische Untersuchungen zur Konservierung von Knochenimplantaten

High demands on freedom from germs are made on auditory ossicles to be used as implants. Postoperative infections often result from the existence of germ-contaminated ossicles. That is why we undertook microbiological examinations, using three frequently applied preserving methods for auditory ossicles kept for tympanoplasties. The results of the examinations were: Disinfection by Cialit (often used in clinical practice) produced unsatisfactory results. Disinfection by ethyl alcohol (70 per cent) and a subsequent treatment with thiomersal-Ringer's solution (0.4 per cent) yielded satisfactory results. Disinfection by preservation with an aqueous solution of formalin and subsequent treatment with Cialit proved most effective. None of the applicated germs could be regrown.

Formalin and footrot in sheep

Changes in formalin concentration were measured over time in uncontaminated solutions and solutions heavily contaminated by mud, faeces, wool or straw. Shallow plastic trays containing 1.11 of a 9% aqueous solution of formalin were exposed for 6 days (trial 1) or 8 days (trial 2) to windy autumn weather. Evaporation of solution volumes was high (47-73%). Concentration generally increased over time: by 13-31% in trial 1 and by 61-162% in trial 2. These figures represent actual increases in percentage formalin of up to 3.0% in trial 1 and up to 7.6% in trial 2. Exposure to mud, sheep faeces or wool did not affect the increase in concentration but there was no increase in the presence of straw. As exposure to excessively concentrated formalin solutions produces hyperaemia, cracking of interdigital skin and lameness in sheep it is recommended that footbath solutions should be diluted to 2-S%, prepared on the day of use and discarded daily whether contaminated or not. Addition of formalin or water to existing solutions of unknown strength should be avoided.

Effects of prolonged moderate body deuteration on proliferative activity in major cell renewal systems in mice

To evaluate the effects of prolonged moderate body deuteration on incorporation of tritiated thymidine ( 3HTdR) into the DNA of major cell renewal systems, young adult mice were given drinking fluid containing 30 % heavy water for 7, 14, 21, 42 and 70 days. Control mice drank tap water. Three hours prior to sacrifice, 925 kBq of 3HTdR were injected intravenously. Following extraction of the bulk of the soluble activity with an aqueous formalin solution, the residual 3H-activity of the organs was assayed by liquid scintillation counting and by autoradiography. The total thymic 3H-activity and the thymic weight, particularly of the cortex, were significantly reduced in deuterated mice early in the course of the experiment. The fraction of labeled thymocytes diminished to less than one half of the control values of day 70. The 3H-activity of the bone marrow in deuterated mice was reduced to about 3 4 of control values. In contrast, the total 3H-activity of the small intestine, as well as mean labeling index and mitotic index of small intestinal epithelia, were not markedly altered in deuterated versus control mice. Drinking water containing 30 % of heavy water did thus not result in generalized, profound and progressive disturbance of 3HTdR incorporation in all the major cell renewal systems in the mouse. The thymus and, to a lesser extent, the bone marrow, were unquestionably affected, but the depression of 3HTdR incorporation did not increase markedly in the course of 10 weeks. In these terms, the toxicity of 30 % heavy water in the drinking fluid appears minor. This is of particular interest since exposure to similar concentrations is known to elicit immunodepressive and/or antineoplastic effects.