Aqueous Enzymatic Oil Extraction Research Articles

Aqueous enzymatic extraction of macauba (Acrocomia aculeata) pulp oil: A green and sustainable approach for high-quality oil production

Aqueous enzymatic extraction (AEE) of macauba pulp oil (MPO) was performed in this study with five commercial enzymatic pools. The chemical, nutritional, and thermal properties of the oils with high oil efficiency by AEE were evaluated and compared with mechanical pressing (MP) and organic solvent extraction (SE). Among the AEE processes, the pectinase pool (at pH 5.5 and 50 °C) exhibited the highest process efficiency (88.6 %). The oils presented low acidity values (0.4–3.1 %) and low molar absorptivities, indicating minimal oil degradation. Bioactive compounds, such as carotenoids, were found in MPO. The iodine index and the fatty acid profile of the oils revealed a high content of unsaturated fatty acids, particularly oleic and linoleic acids, with excellent nutritional scores, as evidenced by anti-atherogenicity and anti-thrombogenicity indices. These findings emphasized that AEE is an eco-friendly approach for extracting high-quality MPO with beneficial health compounds for food products.

Investigation on the emulsification mechanism in aqueous enzymatic extraction of edible oil from Schizochytrium sp.

The interaction between emulsified substances and lipids generates an emulsification system during the extraction of microalgae edible oil by aqueous enzymatic method. This study aimed to resolve the dynamics of interfacial protein adsorption during the extraction of microalgae oil at different enzymatic times and the effect on the stability of the interfacial membrane formed by the proteins based on interfacial effects. At 1.5h of enzymatic hydrolysis, the molecular weights of the proteins/peptides were all below 35 kD. In addition, the protein-peptide structure was loose, with the lowest number of disulfide bonds, peak surface hydrophobicity, the highest number of residues, and disordered lipid acyl arrangement. At the same time, the physical stability of the emulsion was the lowest, and the interfacial membrane rupture was distinct. On excessive enzymatic hydrolysis (at 3.0h), a more uniform interfacial membrane was re-formed on the lipid surface. Protein is the main emulsifying substance in the emulsification system. The addition of protease affects the stability of the interfacial membrane formed by proteins. In addition, sufficient enzymatic hydrolysis (1.5h) inhibited emulsification, while excessive enzymatic hydrolysis (3.0h) promoted emulsification. © 2023 Society of Chemical Industry.

Optimization of ultrasound assisted aqueous enzymatic extraction of oil from Cinnamomum camphora seeds

Cinnamomum camphora seed oil was extracted via ultrasound assisted aqueous enzymatic extraction (UAAEE). Neutral protease was selected for the enzymolysis pretreatment. A Plackett–Burman design and Box–Behnken design were employed to obtain the optimal UAAEE conditions and predict maximum oil yield. The optimal UAAEE conditions, with an actual yield of 80.12 ± 1.35%, were as follows: 4 h incubation time, 400 rpm agitation rate, 10 mL/g water to solid ratio, 1.9% enzyme amount, 1.4 h ultrasound time, and 220 W ultrasound power. Analysis of fatty acid compositions demonstrated that C. camphora seed oil possessed high medium-chain triglyceride content. Analysis of physicochemical properties demonstrated that the oil extracted via UAAEE possessed a lower acid value in comparison with oil extracted using the Soxhlet extraction; furthermore, other indices showed no significant difference between the two methods. The nutritional properties analysis revealed that the oil obtained using UAAEE had remarkable antiatherogenic and hypocholesterolemic activities. These results clarified that UAAEE is an efficient and eco-benign method for extracting plant seed oil and also indicated that C. camphora seed oil is a medium-chain triglyceride oil, which can be used as an ingredient in milk formulae for infants or in other health-related food industry products.

Process optimization in the extract of perilla seed oil with plant protein hydrolysate complex enzyme

Perilla seed oil riched in the ω-3 fatty acid especially α-linolenic acid, is associated with a beneficial role in human health. In the present study, Aqueous Enzymatic Extraction (AEE) of crude oil from Perilla seeds was investigated. The extraction parameters of Perilla seed oil including enzyme type, solid-to-water ratio, pH, temperature, enzyme loading and hydrolysis time were determined by Response Surface Methodology (RSM). The RSM results showed the optimum extraction enzyme for Perilla oil was plant protein hydrolysate complex enzyme. The optimized values of solid-to-water ratio, pH, temperature, enzyme loading, and hydrolysis time for extracting oil from perilla seeds respectively were 1:4, 6, 55 °C, 2% and 4.5 h. Under the conditions, the maximum oil recovery of perilla oil was 88.52%, perilla seed oil extracted by AEE possessed a high extraction yield.

Effect of Aqueous Enzymatic Extraction of Deer Oil on Its Components and Its Protective Effect on Gastric Mucosa Injury.

In this study, deer suet fat was used as a raw material to study the effects of aqueous enzymatic extraction of deer oil on its components, followed by studies into the potential protective activity, and related molecular mechanisms of deer oil on ethanol-induced acute gastric mucosal injury in rats. The results show that aqueous enzymatic extraction of deer oil not only has a high extraction yield and has a small effect on the content of active ingredients. Deer oil can reduce total stomach injury. Without affecting the blood lipid level, it can reduce the oxidative stress, which is manifested by reducing the content of myeloperoxidase (MPO) and enhancing the activity level of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px). It also enhances the expression of defense factors prostaglandin (E2), epidermal growth factor (EGF), and somatostatin (SS), it inhibits apoptosis evidenced by the enhanced of Bcl-2 and decreased expression of cleavage of caspase-3 and Bax. At the same time, it reduces inflammation, which is manifested by reducing the expression of IL-1β, interleukin 6 (IL-6), and tumor necrosis factor alpha (TNF-α) gastric tissue pro-inflammatory cytokines, and enhancing the expression of anti-inflammatory factors IL-4 and IL-10, and inhibiting the mitogen-activated protein kinase/nuclear factor kappa B (MAPK/NF-κB) signaling pathway in gastric tissue.

Aqueous enzymatic extraction of Ricinus communis seeds oil using Viscozyme L

In this study, the extraction of oil from seeds of castor (Ricinus communis) was intended using aqueous enzymatic extraction (AEE). After evaluating several enzymes (cellulase, pectinase, hemicellulase and Viscozyme L), the enzymatic cocktail Viscozyme L gave the best oil extraction yield (64 %) at pH 4 and 50 °C, and it was chosen for the optimization of the extraction process using a response surface methodology and a central composite design. Under optimal conditions (2% of Viscozyme L, 4 h of incubation time, 200 rpm and a solid to liquid ratio of 1:5), an oil yield extraction of 80 % was achieved using AEE. Compared with oil extracted using solvent extraction, AEE produced an oil with a higher acid and iodine values, but a similar fatty acid composition. Additionally, oxidative stability was higher in oil extracted using AEE, so it can be said that in terms of this parameter, AEE produces a higher quality oil compared to the SE. Scanning Electron Micrographs (SEM) imagines showed that Viscozyme L causes a destruction of cell walls and liposome membranes structure of castor seeds, causing the oil release. The results showed that AEE is a no contaminant extraction technology that can be employed in the industrial extraction of oil from castor seed.

Physicochemical properties, fatty acid compositions, bioactive compounds, antioxidant activity and thermal behavior of rice bran oil obtained with aqueous enzymatic extraction

Rice bran as a by-product of rice processing can be used to produce oil. Aqueous enzymatic extraction (AEE) of rice bran oil (RBO) was performed in this study. The physicochemical properties, fatty acid compositions, bioactive compounds, antioxidant activity and thermal behavior of AEE-extracted oil (AEEO) were evaluated and compared with those of Soxhlet-extracted oil (SEO). It was found that iodine value and saponification value in the AEEO were higher than the SEO. AEEO with lower acid value and peroxide value was considered to be a high quality. The contents of wax and phospholipid in AEEO were lower, which could simplify the refining steps. Additionally, AEEO exhibited a higher content of unsaturated fatty acids (76.31%), sum of tocopherols and tocotrienols (1004 mg/kg), sterols (7749 mg/100 g), squalene (2962 mg/kg), oryzanol (2.43 g/100 g). The antioxidant activity of AEEO (EC50 = 5.52 mg/mL) was better than that of SEO (EC50 = 8.66 mg/mL). Moreover, AEEO was found to have lower crystallization and melting points. Scanning electron microscope (SEM) results showed the destruction of cell walls and the decrease of substances in the cell by AEE. Overall, the present results showed the improvement in the quality of the AEEO for green extraction of RBO.

Effect of enzyme on the demulsification of emulsion during aqueous enzymatic extraction and the corresponding mechanism

AbstractBackgrounds and objectivesEmulsion is formed during the aqueous enzymatic extraction (AEE) of peanut oil and protein. Protein and phospholipid, the main emulsifiers, play an important role in the stability of this emulsion. In this study, phospholipase A1, phospholipase A2, phospholipase D, papain, or Alcalase 2.4 L was used as demulsification agents. The phospholipid composition, interfacial protein concentration and composition, viscosity, particle diameter distribution, and microstructure of the emulsion during demulsification were evaluated.FindingsNuclear magnetic resonance analysis showed that the content of phosphatidylcholine decreased by 12.3% after treatment with the three phospholipases, whereas the content of phosphatidic acid increased 9 times. Treatment with protease caused the molecular weight of interfacial proteins and the viscosity of the emulsion significantly decreased, and the average particle diameter increased 8 times.ConclusionsThe free oil yields obtained using PLA1, PLA1/PLA2, PLD, Alcalase 2.4 L, and papain were 45.6%, 85.3%, 30.7%, 84.3%, and 93.8%, respectively. Papain destroyed the interfacial membrane of the emulsion and had the best demulsification effect.Significance and noveltyThe findings solve the problem of difficult demulsification during the extraction of peanut oil by AEE and provide a theoretical basis for the large‐scale industrial application of the demonstrated demulsification method.

Quality parameters of ultrasound assisted aqueous enzymatic extraction of rice bran oil

The quality parameters like free fatty acids, peroxide value, colour and viscosity of conventional (Hexane) and ultrasound assisted aqueous enzymatic extraction of rice bran oil were examined. It was found that the ultrasound extracted oil had lower contents of free fatty acids, colouring matters and had higher contents of peroxide value, viscosity than conventional (Hexane) extracted oil. Hence, in conclusion, ultrasound assisted aqueous enzymatic extraction has the potential to replace the conventional solvent extraction.

Aqueous enzymatic extraction of rice bran oil

An aqueous enzymatic extraction method was developed to extract rice bran oil. Different concentrations of individual enzymes, combinations of enzymes and incubation time were studied. The effect of hexane and aqueous enzymatic extraction on the oil yield was compared. The combination of cellulase (270 U), amylase (216 U) and protease (135 U) for four hours of incubation yielded significantly more oil than the other combinations. Under the optimal reaction conditions, a free oil recovery of 76.48% was obtained. The findings suggest that the critical selection of AEE parameters is key to high oil recovery.

Aqueous Enzymatic Extraction of Oil from Microwave-pretreated Jicaro Seeds

Background: Jicaro seeds can be considered oilseeds due to their high content of lipids, which could be used. Aqueous Enzymatic Extraction (AEE) of oils is a safe, economical and ecological technique, with the disadvantage that oil yields are lower than conventional methods. The microwaves pre-treatment of the seeds has been applied to overcome this inconvenience. Therefore, the objective of this study was to investigate the aqueous enzymatic extraction of oil from Jicaro seed pretreated in a microwave (M-AEE) compared to Soxhlet extraction, in terms of oil yield and its quality. Methods: M-AEE was evaluated varying type of enzyme, extraction time, enzyme concentration, solid/liquid ratio and particle size, according to Fractional Factorial experimental design. The obtained oil was analyzed in terms of fatty acid content and antioxidant activities. The same analyses were performed in oil obtained by Soxhlet extraction for comparison. Results: All parameters investigated in this work had a positive effect on the extraction process. The highest oil yield of 69.71% was obtained with a particle size of 0.59 mm, 3% (w/w) of Viscozyme L, solid/ liquid ratio of 1:6, and 5 h. No differences were found in fatty acid content and antioxidant activity between the oil obtained by M-AEE and that obtained by SE. Conclusion: The properties of the oil were not different in the two extraction methods evaluated; however, M-AEE has the advantage of being environmentally friendly technique. Jicaro seed oil physicochemical properties were similar to edible oils, thus could be considered as a new option of edible vegetable oil.

Optimization of aqueous enzymatic extraction of oil from shrimp processing by-products using response surface methodology

Abstract The aqueous enzymatic extraction (AEE) of oil from shrimp processing by-products was investigated. Four kinds of proteases, including alkaline protease, neutral protease, flavor protease and compound protease, were applied to hydrolysis shrimp processing by-products. The results showed that flavor protease was the best hydrolysis enzyme for shrimp processing by-products to obtain high oil recovery. The influences of four factors, including enzyme amount, liquid/solid ratio, hydrolysis time and hydrolysis temperature, on shrimp oil extraction yield were also studied. The flavor enzyme hydrolysis condition was optimized as following: enzyme amount of 2.0% (w/w), liquid/solid ratio of 9.0ml/g, hydrolysis time of 2.6 h and hydrolysis temperature of 50 °C. Under these optimum hydrolysis conditions, the experimental oil extraction yield was 88.9%.

A Novel Aqueous Extraction for Camellia Oil by Emulsified Oil: A Frozen/Thawed Method

Emulsion is inevitable during the aqueous extraction of oil from oilseeds. In this study, a new aqueous extraction method for camellia oil is investigated. During the extraction, the emulsified oil is collected, frozen, and thawed, and the free oil is obtained (AEEF). The optimal processing conditions are as follows: three times as much water as camellia seed powder (w/w), grinding at 70 °C for 6 min, repeating 2 cycles to collect the emulsified oil; then the emulsified oil is frozen (−20 °C) /thawed (50 °C) for 2 cycles. The yield of camellia oil is 89.37 ± 0.51%, which is significantly higher (p < 0.05) than that of microwave‐assisted aqueous enzymatic extraction (MAAEE, 83.05 ± 1.14%), and is similar to that of cold press extraction (CPE, 90.85 ± 2.05%), as well as solvent extraction (SE) coupled with refining. Additionally, the total oxidation value (TOTOX) of the free oil extracted by using AEEF (AEEF‐Oil, 6.82 ± 0.12%) is significantly lower (p < 0.05) than those of MAAEE, CEP, or SE. In comparison to SE‐Oil and CEP‐Oil, AEEF‐Oil has higher (p < 0.05) contents of α‐tocopherol and squalene, and lower (p < 0.05) benzo(a)pyrene contents, and contains more favorable natural volatile compounds. The results indicate that the novel AEEF may be a promising aqueous extraction method.Practical Application: The novel aqueous extraction method for camellia oil by emulsified oil – frozen/thawed has acceptable oil yields. The obtained camellia oil has high levels of bioactive components, low contents of hazardous substances, and more characteristic volatile flavor compounds. AEEF is an improved aqueous extraction technology for edible oil without refining and using n‐hexane, which could be potentially applied in industrial production of camellia oil due to its safety, nutrition, and environment‐friendly treatments.Aqueous extraction is a promising technique for edible oil extraction from oilseeds. The novel technology of aqueous extraction for camellia oil through emulsified oil – frozen/thawed (AEEF) is investigated. In comparison to solvent extraction, cold press extraction, and microwave‐assisted aqueous enzymatic extraction of camellia oil, the obtained camellia oil by AEEF has an acceptable oil yield, high quality, and nutritional value. AEEF is a safe, simple and environmental‐friendly aqueous extraction technology.

Optimization of the Aqueous Enzymatic Extraction of Oil from Oecopetalum Mexicanum

Optimization of the Aqueous Enzymatic Extraction of Oil from Oecopetalum Mexicanum

Process Optimization for Extraction of Millet Small Bran Oil by Aqueous Ethanol

Millet small bran is a kind of byproduct of the millet processing. It is a cost-effective resource for healthy nutritious oil, which contains 23% oil, with 72% essential fatty acid, tocopherol, phytosterol and oryzanol. In recent years, aqueous enzymatic extraction of millet bran oil has been researched widely. However, this method has the disadvantages of high production costs, low extraction efficiency and great loss of nutrition. In order to reduce the production cost and improve nutritive value of the products, the ethanol extraction method was developed and response surface method (RSM) was used to optimize extraction conditions. The research investigated the influence of extraction temperature, extraction time, solid-liquid ratio and ethanol concentration. The result showed that extraction time, solid-liquid ratio and ethanol concentration made the most contribution to enhancing free oil yield. According to the principle of box–behnken design, response surface analysis of 3 factors and 3 levels was used to optimize the extraction process. The optimal conditions of this aqueous extraction method were: extraction time (120min), solid-liquid ratio (1:5) and ethanol concentration (30%). Under the optimal conditions, the oil yield reached 89.216%. Through determining the quality indices such as acidity, peroxide value and water content of millet small bran oil extracted by aqueous ethanol solution, it could be concluded that the quality of extracted oil was superior to the national standard of crude millet bran oil and similar to the first grade pressed bran oil under the national standard. The contents of tocopherol and phospholipid in millet small bran oil were 131.32mg/100g and 1.12g/100g, respectively. Aqueous ethanol extraction method is not only an efficient and suitable method of extracting millet small bran oil, but also preserve the nutrients like phospholipid and tocopherol to the greatest extent. Hence, it can be concluded that aqueous ethanol technology has a good applying prospect in oil production industry.

Ultrasound-assisted Aqueous Enzymatic Extraction of Corn Germ Oil: Analysis of Quality and Antioxidant Activity.

An efficient method of ultrasound-assisted aqueous enzymatic extraction of corn germ oil was established, and its quality and antioxidant activity were studied. The optimum auxiliary extraction conditions with ultrasound were as follows: the ultrasonic treatment time was 20 minutes, and the ultrasonic temperature was 40°C. The corn germ oil extracted by the aqueous enzymatic method had better quality indexes in comparison with that exacted by the solvent. The fatty acid compositions of corn germ that was oil-extracted by two kinds of methods had almost no significant differences. The tea polyphenols (TP) exhibited remarkable antioxidant effects on corn germ oil during a storage stability test. Meanwhile, we focused on the antioxidant activity of corn germ oil, and the results showed that corn germ oil could effectively scavenge 1,1-diphenyl-2-picrylhydrazyl(DPPH), hydroxyl radical and superoxide anion free radicals. Thus, corn germ oil is a kind of functional oil with excellent antioxidant activity and could be used as a free radical scavenger in the food or other industries.

Secondary structure of proteins on oil release in aqueous enzymatic extraction of rapeseed oil as affected hydrolysis state

ABSTRACTIn this study, the relationship between protein hydrolysis and oil release was studied by fluorescence microscopy and Fourier Transform Infrared (FTIR) spectroscopy, respectively. Five commercial proteases (Flavourzyme 1000L, Neutrase 1.5MG, Protamex, Alcalase 2.4L, and Thermolysin) were evaluated for oil extraction from rapeseed, and Alcalase 2.4L was found to be the best enzyme for highest free oil yield (81.81%). When use this enzyme, the results showed that parts of proteins restricted the release of oil, and the oil released almost completely was achieved after hydrolysis for 3.0 h. To further reveal the relationship between oil release and protein hydrolysis, the effects of hydrolysis time on the degree of hydrolysis of protein, and free oil yield were studied. The results showed that the release of oil was only related to the hydrolysis of parts of proteins, and when the degree of hydrolysis of protein was more than 17.05%, the effect of protein hydrolysis on oil release was greatly reduced. The intrinsic relationship between the structural changes of proteins and the release of oil in the hydrolysis process was analyzed by comparing protein secondary structure of different hydrolysis time, and the results showed that the transition of protein structure from order to disorder was an important factor for oil release.

Ultrasound-assisted aqueous enzymatic extraction of oil from perilla seeds and determination of its physicochemical properties, fatty acid composition and antioxidant activity

Ultrasound-assisted aqueous enzymatic extraction of oil from perilla seeds and determination of its physicochemical properties, fatty acid composition and antioxidant activity

High pressure pre-treatment of Moringa oleifera seed kernels prior to aqueous enzymatic oil extraction

Abstract High pressure processing (HPP) was applied as a pre-treatment on Moringa oleifera (MO) kernels, for the first time, prior to aqueous enzymatic extraction (AEE) of the MO oil, and the effect of this pre-treatment is reported in terms of the free oil recovery and the nature of the cream emulsions formed. The HPP pre-treatments (50–250 MPa, 20–60 °C, 10–60 min) generally resulted in higher free oil recoveries and thinner emulsion layers from ground-sieved kernels than the whole kernels. Optimization of the HPP parameters indicated linear increment in free oil recovery with increase in temperature and time, but not the pressure level. Without the pre-boiling step in the AEE process, the use of HPP pre-treatment at 50 MPa and 60 °C for 35 min resulted in approximately 73% (w/w) free oil recovery with thinner emulsion layer than the use of AEE alone. These findings highlighted the ability of HPP in altering the MO protein structure into a form of less emulsifying functional properties, thus further de-emulsification method may not be necessary. Industrial relevance High pressure processing (HPP) applications are increasing in the food industry. High pressure application allows the use of relatively lower temperatures in processes, in order to achieve comparable outcomes of higher temperature processing. This study explores the application of HPP on Moringa oleifera (MO) kernels prior to aqueous enzymatic extraction (AEE) of oil. In general, the main disadvantage of AEE is its lower oil recovery in comparison with solvent extraction, which is attributed to the formation of a relatively stable cream emulsion after extraction. This study shows that the thickness of the creamy emulsion can be significantly reduced, and oil recoveries improved, by subjecting the kernels to HPP prior to extraction. The improvement in recoveries did not depend strongly on the level of high pressure applied, but downstream processes for free oil separation and recovery were considerably simplified.

Ultrasound-Assisted Aqueous Enzymatic Extraction of Oil from Pomegranate Seeds

Pomegranate seed oil presents nutraceutical properties. Industrial processes for extraction of edible oils from seeds generally involve a solvent extraction step. Safety considerations on the use of organic solvents prompted attempts to develop aqueous extraction. However, aqueous extraction processes are usually characterized by low oil yields. The objective of this work was to overcome these low yields by using enzymes. The pomegranate seeds were treated with two enzymes—cellulase and Peclyve V. The extraction temperature, the time, the enzyme concentration, and the water/seeds ratio were varied between 35 and 55 °C, 2 and 8 h, 2 and 4 % w/w, and 2/1 and 6/1 mL/g, respectively. The optimum yield accomplished (15.33 g oil/100 g dry seeds at 2 h or 81 % oil recovery) was comparable to the yields obtained by other extraction methods, such as normal stirring, cold pressing, superheated fluid extraction, indirect ultrasound-assisted extraction and supercritical extraction, (4.29–25.11 g oil/100 g seeds) at similar or longer extraction times (10 min–72 h). In addition, this work studies the enhancement of aqueous enzymatic extraction of pomegranate seed oil by ultrasound probe. It was found that only 10 min in water are needed to recover oil with a yield of 18.15 g oil/100 g dry seeds, a value similar to or higher than those of the conventional procedures. Thus, the use of ultrasounds increased the yield of enzymatic extraction by 18.4 % and reduced the extraction time by 91.7 %.