Abstract
An aqueous enzymatic extraction method was developed to extract rice bran oil. Different concentrations of individual enzymes, combinations of enzymes and incubation time were studied. The effect of hexane and aqueous enzymatic extraction on the oil yield was compared. The combination of cellulase (270 U), amylase (216 U) and protease (135 U) for four hours of incubation yielded significantly more oil than the other combinations. Under the optimal reaction conditions, a free oil recovery of 76.48% was obtained. The findings suggest that the critical selection of AEE parameters is key to high oil recovery.
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