Aquatic Food Products Research Articles

Proteomic Applications in Aquatic Environment Studies.

Genome determines the unique individualities of organisms; however, proteins play significant roles in the generation of the colorful life forms below water. Aquatic systems are usually complex and multifaceted and can take on unique modifications and adaptations to environmental changes by altering proteins at the cellular level. Proteomics is an essential strategy for exploring aquatic ecosystems due to the diverse involvement of proteins, proteoforms, and their complexity in basic and advanced cellular functions. Proteomics can expedite the analysis of molecular mechanisms underlying biological processes in an aquatic environment. Previous proteomic studies on aquatic environments have mainly focused on pollution assessments, ecotoxicology, their role in the food industry, and extraction and identification of natural products. Aquatic protein biomarkers have been comprehensively reported and are currently extensively applied in the pharmaceutical and medical industries. Cellular- and molecular-level responses of organisms can be used as indicators of environmental changes and stresses. Conversely, environmental changes are expedient in predicting aquatic health and productivity, which are crucial for ecosystem management and conservation. Recent advances in proteomics have contributed to the development of sustainable aquaculture, seafood safety, and high aquatic food production. Proteomic approaches have expanded to other aspects of the aquatic environment, such as protein fingerprinting for species identification. In this review, we encapsulated current proteomic applications and evaluated the potential strengths, weaknesses, opportunities, and threats of proteomics for future aquatic environmental studies. The review identifies both pros and cons of aquatic proteomics and projects potential challenges and recommendations. We postulate that proteomics is an emerging, powerful, and integrated omics approach for aquatic environmental studies.

Commoning the governance: a review of literature and the integration of power

The concept of commoning is continuing to gain scholarly interest, with multiple definitions and interpretations across different research communities. In this article, we define commoning as the actions by groups with shared interests towards creating shared social and relational processes as the basis of governance strategy. Perhaps it can be more simply defined as collective ways of relating and governing. This article addresses two specific gaps in the commoning literature: (1) to bridge disparate strands of literature on commoning by briefly reviewing each and arguing for integration through epistemic pluralism, and (2) to explicitly examine how power is manifest in commoning processes by bringing in a framework on power (i.e., power over, power with, power to, power within) to understand the links between power and commoning governance processes in two case studies. The two cases are tourism governance on Gili Trawangan, Indonesia and aquatic food production systems in Bulacan, Philippines. We preface this analysis with the argument that power is an integral part of the commoning concept, but that it has yet to be analytically integrated to applications of the broader institutional analysis and development framework or within the networks of action situations approach. We argue that by making explicit how an analysis of power can be coupled to a network of action situations analysis in a qualitative way, we are advancing a key feature of the commoning concept, which we introduce as rooted in epistemic and analytical pluralism in the analysis of governance. In the discussion, we expand on how each case study reveals each of the four power dynamics, and how they improve the understanding of commoning as a pluralistic and perhaps bridging analytical concept.

Global food insecurity and famine from reduced crop, marine fishery and livestock production due to climate disruption from nuclear war soot injection

Atmospheric soot loadings from nuclear weapon detonation would cause disruptions to the Earth’s climate, limiting terrestrial and aquatic food production. Here, we use climate, crop and fishery models to estimate the impacts arising from six scenarios of stratospheric soot injection, predicting the total food calories available in each nation post-war after stored food is consumed. In quantifying impacts away from target areas, we demonstrate that soot injections larger than 5 Tg would lead to mass food shortages, and livestock and aquatic food production would be unable to compensate for reduced crop output, in almost all countries. Adaptation measures such as food waste reduction would have limited impact on increasing available calories. We estimate more than 2 billion people could die from nuclear war between India and Pakistan, and more than 5 billion could die from a war between the United States and Russia—underlining the importance of global cooperation in preventing nuclear war.

Nutritional quality traits of Mediterranean mussels (Mytilus galloprovincialis): A sustainable aquatic food product available on Italian market all year round.

In this study, Mediterranean mussels (Mytilus galloprovincialis) coming from Italian production were purchased in the most important Italian wholesale fish market in different seasons. Biometrical parameters and chemical composition were investigated, with a particular focus on lipid quality and fatty acids (FAs) composition. Results showed a valuable nutritional profile independently by the season of production, represented by high protein and low-fat content, with the lipid portion represented by high amounts of beneficial FAs, particularly the long chain of the n-3 series. Some differences (p < 0.05) were found in carbohydrates and fat content of mussels edible tissues and in FAs profile of specimens collected in different seasons. The most favourable composition in terms of lipid quality was found in mussels collected during spring, corresponding to the moment of the year when mussels store energy reserves in the form of carbohydrates and fat (preparing for the spawning events) and when seawater is enriched in phytoplankton. The lipid health indices calculated (n6/n3, AI, TI) showed optimal values independently by the season of production. The outcomes obtained in this study could help supporting the appreciation of Mediterranean mussels as nutritional valuable seafood product, thus helping encouraging their consumption and promoting the appraisal of this farming sector essential for Italian aquaculture and related to low environmental impact.

Evaluation of safety and withdrawal period of orally administered Oxytetracycline (OTC) in advance fingerlings of Cirrhinus mrigala (Hamilton, 1822)

The present study reports the safety and withdrawal period of orally administered oxytetracycline in advance fingerlings of Cirrhinus mrigala. For evaluation of safety, the drug was administered as feed-top dressing @ 80, 240, 400 and 800 mg kg−1 fish biomass per day consecutively for 30 days. Samples of liver, kidney, gills were collected at ten days intervals for histopathological examination. For determination of withdrawal period, fish were administered orally at the therapeutic dose of 80 mg kg−1 day−1 consecutively for ten days, and an edible portion (muscle-skin) was collected for estimation of residue levels at 48 h intervals after the termination of medication. The animal behaviour was observed daily, and growth was measured once in 10 days. The drug was well tolerated up to 240 mg kg−1 while fish administered with higher doses (400 mg and 800 mg kg−1 day−1) showed growth retardation. Histopathological examination revealed mild to moderate changes in the gills, liver and kidney in the group receiving 400 and 800 mg kg-1 concentration of OTC. Residue analysis using LC-MS/MS showed the highest concentration of the drug in the liver (13.53 mg kg−1) followed by the muscle (296.00 μg kg−1) on 5th day while in kidney (287.40 μg kg−1) it was on 13th day. On the ninth day, after the cessation of medicated feeding, the tissue residue (189.4 μg kg−1) was below the maximum residual limit (MRL) prescribed by the FAO Codex Alimentarius (200 μg kg−1) in the edible portion. These results help tropical countries like India to determine the safe dose and appropriate withdrawal time to ensure the safety of aquatic food products.

Advantage of Species Diversification to Facilitate Sustainable Development of Aquaculture Sector.

Simple SummaryThe aquaculture sector must be well-founded to undergo robust growth and sustainable development in the years ahead. Species diversity must reflect species compatibility and complementarity to manage the complexity in polyculture systems. There is a need for the implementation of innovative strategies that facilitate sustainable aquaculture development, enhance profitability, improve resilience, and support conservation and environmental protection. An aquaculture development scenario must look beyond the economic profitability and strategize aquatic food production systems to attain food and nutrition security and benefits for all stakeholders.Intensified agrochemical-based monoculture systems worldwide are under adoption to meet the challenge of human population growth and the ever-growing global demand for food. However, this path has been opposed and criticized because it involves overexploitation of land, monoculture of few species, excessive input of agrochemicals, and adverse impacts on human health and the environment. The wide diversity among polyculture systems practiced across the globe has created confusion over the priority of a single strategy towards sustainable aquaculture development and safer products. Herein, we highlight the significance of polyculture and integrated aquaculture practices in conveying the successful transition of the aquaculture industry towards sustainable development. So far, the established thought is that the precise selection of aquatic species and a focus on compatible and complementary species combinations are supposed to facilitate rapid progress in food production with more profitability and sustainability. Therefore, the advantages of species diversification are discussed from an ecological perspective to enforce aquaculture expansion. This account asserts that a diverse range of aquaculture practices can promote synergies among farmed species, enhance system resilience, enable conservation, decrease ecological footprints, and provide social benefits such as diversified income and local food security.

Biopolymer‐based antimicrobial coatings for aquatic food products: A review

Aquatic food products, including fish and crustaceans, are some of the most consumed foods globally and are highly prone to microbial contamination. Such products have been preserved using conventional processing techniques such as freezing, cold storage, modified atmospheric packaging (MAP), and vacuum packaging. However, these techniques have been used since decades and are not cost-effective. Therefore, alternative sustainable strategies need to be explored. One viable option is the application of biopolymer-based films and coatings loaded with active antimicrobial agents (peptides and essential oil components) for the preservation of aquatic food products. Nisin is the most widely used peptide for the development of antimicrobial coatings, while eugenol, carvacrol, and cinnamaldehyde are among the most popular essential oil compounds. Findings reveal that both peptides and essential oils, when applied in combination within a coating system, demonstrate robust antimicrobial activity, delayed lipid oxidation, and retain the overall quality of the aquatic food system. Novelty impact statement Antimicrobial-based coating systems have recently gained worldwide attention due to their ability to delay food contamination and maintain product quality throughout the storage period. This review provides a comprehensive description of peptide and essential oil-loaded coating systems and their application in the shelf-life extension of aquatic food products. In addition, the application of nanocomposite systems for the preservation of aquatic foods has been discussed.

The contribution of fisheries and aquaculture to the global protein supply

The contribution of aquatic animal protein to the global, animal-source protein supply and the relative importance of aquaculture to capture fisheries in supplying this protein is relevant in assessments and decisions related to the future of aquatic food production and its security. Meat of terrestrial animals, milk, and eggs resulted in 76,966 Kt crude protein compared with 13,950 Kt or 15.3% from aquatic animals in 2018.While aquaculture produced a greater tonnage of aquatic animals, capture fisheries resulted in 7,135 Kt crude protein while aquaculture yielded 6,815 Kt. Capture fisheries production has not increased in the past two decades, and aquaculture production must increase to assure the growing demand for fisheries products by a larger and more affluent population. We estimated based on status quo consumption, that aquaculture production would need to increase from 82,087 Kt in 2018 to 129,000 Kt by 2050 to meet the demand of the greater population. About two-thirds of finfish and crustacean production by aquaculture is feed-based, and feeds for these species include fishmeal and fish oil as ingredients. Aquaculture feeds require a major portion of the global supply of fishmeal and fish oil. An estimated 71.0% of fishmeal and 73.9% of fish oil are made from the catch with the rest coming from aquatic animal processing waste. The catch of small, pelagic fish from the ocean is not predicted to increase in the future. Aquaculture should reduce its fishmeal and oil use to lessen its dependency on small wild fish important to the integrity of marine food webs and food security for the poor in many coastal areas. Fishmeal and fish oil shortages for use in aquaculture feed will result in a limit on production in the future if goals to lessen their use in feeds are not met.

Optimization of aquaculture sustainability through ecological intensification in China

AbstractAs the world's leading producer of farmed aquatic food products, China faces great uncertainty concerning the further sustainable development of its aquaculture industry due to high and increasing pressure from environmental and resource constraints. To realize truly sustainable development, it is imperative to establish an evaluation system that integrates social, economic, environmental and resource criteria, and optimizes aquaculture systems through a holistic approach. Here, we used analytical hierarchy processes combined with expert judgment and objective data to assess the sustainability of 10 major aquaculture production systems (APSs) in China. Based on the evaluation results, we propose the ecological intensification of aquaculture systems (ELIAS), rationally integrating anthropogenic inputs with aquaculture ecosystem services, to improve the sustainability of APSs. The holistic evaluation system and ELIAS are fundamental and critical for the future development of aquaculture in China and globally.

Application of sous vide cooking to aquatic food products: a review

With the development of cooking technology towards a more quantitative science, consumers have begun to consider cooking as no longer limited to traditional methods. Through continuous recognition, the concept of molecular cooking has gradually entered the mainstream. In addition to the traditional way of conceiving cooking, and on the premise of ensuring food safety and adequate nutrition, food components considered at the molecular level and the use of modern cooking equipment in the preparation of innovative dishes can be combined by employing physical, chemical, and biological strategies. The sous vide (SV) cooking method refers to cooking using precisely controlled temperature and time of vacuum-packed food, which results in a food that is safe, nutritious, convenient, and easy to prepare. As a result, healthy SV preparations are widely accepted among families as well as single and elderly people. This technology has been widely used in catering, food retail, and health food market. In this review, important aspects of the origin and development of SV technology as well as its impact on the quality of aquatic products are discussed. In addition, current existing problems, and prospects to better promote SV technology in aquatic product processing are highlighted. Collectively, this review provides references for the application of SV cooking technology to the industrial processing of aquatic products.

Institutions and institutional changes: aquatic food production in Central Luzon, Philippines

Aquaculture is the most rapidly growing food production sector globally. In certain coastal social-ecological systems, this has resulted in significant changes and sustainability challenges. In particular, coastal environments which used to support only capture fisheries are becoming sites for brackish water aquaculture production; this impacts the sustainability of aquatic food production. Sustainability challenges associated with aquaculture expansion and intensification necessitate a contextually rooted understanding of institutions and institutional changes which can be used as an informed basis for leveraging institutions to achieve desirable sustainability outcomes in the aquatic food sector. This research used a qualitative empirical case study involving in-depth interviews, participant observation, and analysis of institutional documents in the region of Central Luzon, Philippines. It applied the inter-institutional systems concept which considers multiple institutions with distinct but linked purposes and functions in the societal spheres of state, market, and civil society. The study found that aquaculture emerged as an important livelihood because of rice farmers’ need to adapt to saltwater intrusion into what were formerly rice farms. It grew into an industry due to developments in the availability and accessibility of inputs such as fingerlings and feeds. This process was also driven by the high demand and high profitability of fish farming at the time. Regulatory institutions have not adequately adapted to protect the environment. Market institutions adapted but the changes mostly benefited consignacions (middlemen) and large-scale players. However, organised groups of collaborating smallholder fishers and fish farmers are helping to address the disadvantages they face.

Environmental controls of harmful cyanobacterial blooms in Chinese inland waters

Harmful cyanobacterial blooms (CyanoHABs) are expanding world-wide, adversely affecting aquatic food production, recreational and tourism activities and safe drinking water supplies. China's inland waters have been increasingly threatened by CyanoHABs during the past several decades. The environmental factors controlling CyanoHABs are highly variable in space and time in China due to significant variations in climate, geography, geological and geochemical conditions among its many regions. Here, we synthesize diverse examples among Chinese water bodies regarding interactive effects of anthropogenic, climatic and geographic drivers influencing CyanoHAB potentials and dynamics in lakes and reservoirs; in order to provide a perspective and integrative approach to mitigating CyanoHABs. In China's many shallow water bodies, water quality is highly susceptible to human activity and to changing climatic and hydrological conditions, when compared to deeper lakes. Rapid increases in population, economic activity, and wastewater have accelerated CyanoHABs in China since 1980s, especially in the heavily urbanized, agricultural and industrial regions in the middle and lower Yangtze River basins. Climatic changes have provided an additional catalyst for expansion of CyanoHABs. In particular, rising spring temperatures have accelerated the onset and proliferation of Microcystis spp, blooms in the middle and lower reaches of Yangtze River basin. Large hydroelectric and water supply projects, like the Three Gorges Reservoir (TGR), have altered hydrological regimes, and have led to an increase of CyanoHABs in reservoirs and tributaries due to increases in water residence times. Manipulating water level fluctuations in the TGR may prove useful for controlling CyanoHAB in its tributary bays. Overall,CyanoHAB mitigation strategies will have to incorporate both N and P input reductions in these shallow systems. Furthermore, nutrient reduction strategies must consider climate change-induced increases in extreme weather events, including more intense rainfall and protracted heat waves and droughts, which can extend the magnitudes and duration of CyanoHABs. Ensuring the maintenance of natural hydrologic connectivity between lakes and rivers is of utmost importance in mitigating CyanoHABs throughout China.

Recent advances in bio-preservatives impacts of lactic acid bacteria and their metabolites on aquatic food products

Aquatic food products (AFP) are a valuable source of nutrition but are highly perishable and prone to spoilage. Generally, AFP deteriorates by enzymatic, microbiological and oxidative spoilage. Lactic acid bacteria (LAB) are an important class of microorganisms that produce several metabolites showing bio-preservative abilities. Several strains of LAB are well-known to fulfil the requirements for their application as bio-preservatives due to the GRAS status. Thus, LAB has exhibited excellent antimicrobial activity and positive interactions to reduce enzymatic and oxidative spoilage when used in combination with other techniques in the optic of hurdle technologies. This review describes the action of LAB and their metabolites for preservation of AFP as well as their impacts on aquatic food quality and safety. Their possible inclusion with other preservation methods as hurdle techniques is also reviewed.

Withdrawal Interval Estimation of Doxycycline in Yellow Catfish (Pelteobagrus fulvidraco) Using an LC-MS/MS Method Based upon QuEChERS Sampling Preparation.

Limited information on residue depletion of doxycycline (DC) is available in yellow catfish (Pelteobagrus fulvidraco) using the determination of LC-MS/MS based upon a rapid and simple method of sample preparation. This study collected plasma and tissue samples of yellow catfish at pre-determined time points following 3-day consecutive oral administration of DC at 20 mg/kg. The samples were prepared using a QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) approach. The limit of detection and the limit of quantitation were 2.5 and 5 µg/kg or µg/L, respectively, for DC in plasma and tissues (e.g., muscle + skin, liver, kidney, and gill). The recoveries of DC ranged from 67.2% to 86.2%. The decision limit (CCα) and the detection capability (CCβ) were from 106.2 to 127.8 μg/kg or μg/L. The withdrawal times of DC in muscle + skin were estimated to be 22 d based on the guidelines in China and Europe and 27 d based on Japan’s standard. Overall, this study not only provides an efficient method to rapidly determine the DC concentrations in fish-derived tissues but also provides important information on the safety assessment of DC in aquatic animal-derived food products.

Impacts of COVID-19 on the Aquatic Environment and Implications on Aquatic Food Production

The COVID-19 pandemic, caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), resulted in ecological changes of aquatic ecosystems, affected the aquatic food supply chain, and disrupted the socio-economy of global populations. Due to reduced human activities during the pandemic, the aquatic environment was reported to improve its water quality, wild fishery stocks, and biodiversity. However, the sudden surge of plastics and biomedical wastes during the COVID-19 pandemic masked the positive impacts and increased the risks of aquatic pollution, especially microplastics, pharmaceuticals, and disinfectants. The transmission of SARS-CoV-2 from wastewater treatment plants to natural water bodies could have serious impacts on the environment and human health, especially in developing countries with poor waste treatment facilities. The presence and persistence of SARS-CoV-2 in human excreta, wastewaters, and sludge and its transmission to aquatic ecosystems could have negative impacts on fisheries and aquaculture industries, which have direct implications on food safety and security. COVID-19 pandemic-related environmental pollution showed a high risk to aquatic food security and human health. This paper reviews the impacts of COVID-19, both positive and negative, and assesses the causes and consequences of anthropogenic activities that can be managed through effective regulation and management of eco-resources for the revival of biodiversity, ecosystem health, and sustainable aquatic food production.

Aquatic foods to nourish nations.

Despite contributing to healthy diets for billions of people, aquatic foods are often undervalued as a nutritional solution because their diversity is often reduced to the protein and energy value of a single food type ('seafood' or 'fish')1-4. Here we create a cohesive model that unites terrestrial foods with nearly 3,000 taxa of aquatic foods to understand the future impact of aquatic foods on human nutrition. We project two plausible futures to 2030: a baseline scenario with moderate growth in aquatic animal-source food (AASF) production, and a high-production scenario with a 15-million-tonne increased supply of AASFs over the business-as-usual scenario in 2030, driven largely by investment and innovation in aquaculture production. By comparing changes in AASF consumption between the scenarios, we elucidate geographic and demographic vulnerabilities and estimate health impacts from diet-related causes. Globally, we find that a high-production scenario will decrease AASF prices by 26% and increase their consumption, thereby reducing the consumption of red and processed meats that can lead to diet-related non-communicable diseases5,6 while also preventing approximately 166 million cases of inadequate micronutrient intake. This finding provides a broad evidentiary basis for policy makers and development stakeholders to capitalize on the potential of aquatic foods to reduce food and nutrition insecurity and tackle malnutrition in all its forms.

Cold plasma for the preservation of aquatic food products: An overview.

Cold plasma (CP) is an upcoming technology implemented for the preservation of highly perishable foods, especially aquatic food products (AFPs). The high moisture content, high-quality protein with all essential amino acids and unsaturated fatty acids makes AFP more susceptible to microbial spoilage and oxidation of lipids and proteins. Spoilage lowers the nutritive value and could generate toxic components, making it unsafe for consumption. In recent times, the rising demand for food products of aquatic origin with preserved quality and extended shelf-life has been recorded. In addition, minimally or nonthermally processed and preserved foods are gaining great attention. CP technology has demonstrated an excellent ability to inactivate microorganisms without promoting their resistance and triggering some deteriorative enzymes, which are typical factors responsible for the spoilage of AFP. Consequently, CP could be recommended as a minimal processing intervention for preserving the quality of AFP. This review focuses on different mechanisms of fish spoilage, that is, by microorganisms and oxidation, their inhibition via the application of CP, and the retention of quality and shelf-life extension of AFP.

Microplastics contamination in food and beverages: Direct exposure to humans.

Since microplastics (MPs) bring the potential risks to human health when plastics are ingested, more needs to be known about the presence and abundance of human ingestion of MPs. To address these issues, we reviewed 108 publications in Web of Science concerning abundances, sources, and analytical methods of MPs in human daily intake including fish, salt, drinking water, beverages, package food, and other food. The results demonstrate that aquatic food products (fish and bivalves) present a wide range of 0-10.5 items/g for bivalves and 0-20 items/individual for fish. Salt data in literatures present a concentration of 0-13,629 particles/kg. Drinking water is also a pathway of MPs exposure to human, presenting a concentration range from 0 to 61 particles/L for tap water and 0 to 6292 MPs/L for bottled water. Besides, MPs have been found in beverages, package food, sugar, honey, vegetables, and fruits. Therefore, human intake of MPs via ingestion is a nonnegligible exposure route.

Developing Climate Information Services for Aquaculture in Bangladesh: A Decision Framework for Managing Temperature and Rainfall Variability-Induced Risks

Climate information services (CIS) are increasingly in demand to assist farmers in managing risks associated with climate variability and extremes experienced in food production. However, there are significant gaps in the availability and accessibility of these services, especially in aquatic food production in developing countries. In response, this study aims to generate the background knowledge for developing climate information and decision support services tailored for aquaculture farmers in Bangladesh. We surveyed 800 fish-farming households, interviewed 30 key informants, and conducted a systematic literature review to identify climate-sensitive operations and management decisions in aquaculture and to document fish-farmers' awareness of the relationships between climate variability and aquatic food production systems. We also sought to identify the lead time and communication method(s) needed to deploy forecasts effectively and prepare aquaculture farmers to act in response to the forecasts. A fish-farming activity calendar was developed that identified high temperature, cold spell, heavy rainfall, and dry spell events as key climatic phenomena affecting year-round aquaculture operations, including pond preparation and maintenance, fingerling stocking, grow-out management, and harvesting. We also identified five climate-sensitive management decision points and 26 potential advisories in line with specific climate variability to manage induced risks in the day-to-day operations of fish farmers. Finally, the research team developed a decision framework based on the temperature and rainfall thresholds for the grow-out phase of four widely cultivated and economically important fish species in Bangladesh. This innovative decision support approach is to our knowledge the very first endeavor to develop CIS using species-specific temperature and rainfall thresholds to reduce climate risks and ensure resilience capacity for South Asian aquaculture system.

Characterization of Iturin V, a Novel Antimicrobial Lipopeptide from a Potential Probiotic Strain Lactobacillus sp. M31

Members of lactic acid bacteria group are known to produce various antimicrobial substances. Cyclic lipopeptides are one such potent class of amphipathic natural biosurfactants that exhibit bactericidal and immunomodulatory properties. In this study, we aimed to investigate antimicrobial and immunomodulatory activities of a lipopeptide secreted by a LAB isolate strain M31 identified as a member of the genus Lactobacillus. The lipopeptide that was purified using a combination of chromatographic techniques and matrix-assisted laser desorption/ionization-time of flight of pure lipopeptide displayed a molecular weight of 1002Da. MS/MS analysis confirmed the presence of 7 amino acids (Asp-Tyr-Asp-Val-Pro-Asp-Ser) and a C13 beta-hydroxy fatty acid. The amino acid composition assigned lipopeptide to iturin class. However, the replacement of Gln with Val revealed it to represent a novel iturin named as iturin V. Iturin V showed antibacterial activity and did not cause hemolysis or cytotoxicity upto 125µg/mL. It induced secretion of pro-inflammatory cytokines TNF-alpha and IL-12 in murine dendritic cells. Probiotic features of strain M31 coupled with notable activity of iturin V against species of the genera Pseudomonas and Vibrio suggest that strain M31 has potential application for pathogen intervention treatments in processing of aquatic food products.