The well-defined physical, chemical, and biological interactions that define the behaviour of biosensors are described by nonlinear differential equations. Because these biosensors have so many uses in many domains, it is desirable to represent them mathematically. The enzyme kinetic and the diffusion rate across the enzymatic layer determine the distinct enzymatic processes. The biosensors are modelled by a system of reaction-diffusion equations involving a nonlinear part associated with the reaction rate. The nonlinear equations in this model have been solved analytically (Rajendran-Joy method) and numerically (MATLAB® v2016b software). Approximate steady-state analytical solutions for the substrate and reaction product concentrations and the output current are presented for specific cases of first-order, Michaelis-Menten and ping-pong kinetics. The influence of various parameters on concentrations and currents is discussed. Further, the sensitivity of parameters in current was also analysed. The analytical results are compared with numerically simulated results for various kinetics.