Regenerative Medicine Applications Research Articles

Visualizing Trends and Bibliometric Study in Tissue Engineering for Rotator Cuff Injuries.

This research is dedicated to uncovering the evolving trends, progressive developments, and principal research themes in tissue engineering and regenerative medicine for rotator cuff injuries, which spans the past two decades. This article leverages visualization methodology to provide a clear and comprehensive portrayal of the dynamic landscape within the field. We compiled 758 research entries centered on the application of tissue engineering and regenerative medicine in treating rotator cuff injuries, drawing from the Web of Science Core Collection database and covering the period from 2003 to 2023. Analytical tools such as VOSviewer, CiteSpace, and GraphPad Prism were used. We conducted comprehensive analyses to discern the general characteristics, historical evolution, key literature, and pivotal keywords within this research field. This comprehensive analysis enabled us to identify emerging focal points and current trends in the application of tissue engineering and regenerative medicine for addressing rotator cuff injuries. The compilation of 758 articles in this study indicates a consistent upward trajectory in publications concerning tissue engineering and regenerative medicine for rotator cuff injuries. The scholarly contributions from the United States, China, and South Korea have notable influence on the progression of this research area. The analysis delineated ten specific research subdomains, including fatty infiltration, tears, tissue engineering, shoulder pain, tendon repair, extracellular matrix (ECM), and platelet-rich plasma growth factors. Noteworthy is the recurrent mention of keywords such as "mesenchymal stem cells," "repair," and "platelet-rich plasma" throughout past two decades, highlighting their critical role in the evolution of the relevant field. This bibliometric analysis meticulously examines 758 publications, offering an in-depth exploration of the developments in tissue engineering and regenerative medicine for rotator cuff injuries between 2003 and 2023. The study effectively constructs a knowledge map, delineating the progressive contours of research in this domain. By pinpointing prevailing trends and emerging hotspots, the study furnishes crucial insights, setting a direction for forthcoming explorations and providing guidance for future researchers in this evolving field.

Stem-cell therapy in dermatology – Challenges and opportunities

The remarkable characteristics of stem cells (SCs), particularly their potency, capacity for self-renewal, and clonality, are well known. In recent years, there have been notable advancements in the field of cell therapy. Due to its distinctive qualities and versatility, this innovative therapy is already turning into a potentially helpful remedy for several diseases. Numerous dermatological problems, including autoimmune skin disorders, atopic dermatitis, skin aging, wound healing, scar repair, and many more, have been treated with SC-based therapies. Their application in regenerative medicine and esthetic dermatology is based on their capacity to self-renew and differentiate into distinct cell types. Given their abundance and relative simplicity of collecting adipose-derived SCs have shown to be particularly appealing in terms of appearance. SC therapy has demonstrated encouraging results in both skin regeneration and androgenetic alopecia treatment. Despite favorable preclinical evidence and clinical investigations, further randomized, controlled trials and treatment standardization are required to fully comprehend the role of SCs in the treatment of dermatological conditions, especially esthetics. The purpose of this article is to provide a general review of cell-based therapy, including its current uses, limits, and future possibilities in the field of dermatology.

The liver organoid's past, present and future: a personalized medicine strategy

Organoids are three-dimensional (3D) cell culture systems derived from human pluripotent stem cells or organotypic differentiation, replicating the complex interactions and functionalities of actual organs. These systems offer significant advantages for studying human tissue and organ biology, addressing limitations of animal models related to sample accessibility and ethical concerns. Liver organoids, in particular, are advanced models developed to study hepatic phenotypes, encompassing various cell types and enabling detailed investigation of cellular, molecular, and genetic aspects of liver diseases, drug metabolism, and protein secretion. They hold promise for fundamental research, drug discovery, and regenerative medicine applications. Despite their potential, organoids face limitations such as simplicity, lack of high-fidelity cell types, flexibility, and atypical physiology. Enhancements in organotypic liver-like surrogates, incorporating in vivo-like cell interactions and architecture, along with advancements in microfluidic chip technology, are expected to improve models for disease, toxicity, and drug discovery, paving the way for new treatments. This review will provide an overview of the history and development of liver organoids, their current progress, challenges, applications, and future prospects in the field of personalized medicine.

Light-based and cost-effective bioprinting of musculoskeletal GelMA constructs enriched with mesoporous bioactive glass nanoparticles

Bioprinting represents a promising technique for fabricating three-dimensional (3D) constructs with high-resolution and controlled architecture. However, many bioprinting technologies rely on expensive extrusion systems, which may compromise cell viability due to harsh processing conditions. This study presents the fabrication and characterization of musculoskeletal tissue in gelatin methacryloyl [GelMA]-based nanocomposite 3D constructs (comprising GelMA and mesoporous bioactive glass nanoparticles [MBGNs]) using a cost-effective, light-based bioprinting (LBB) system. We demonstrated that our strategy can produce high-resolution constructs (approximately 250 μm) while maintaining high cell viabilities (above 85%) for extended periods (weeks of culture). Furthermore, the nanocomposite constructs could facilitate the maturation of musculoskeletal tissue derived from C2C12 cells, as indicated by assessments of cell viability, elongation, and alignment over time. Our results suggested that the bioprinting approach outlined in this study allows for precise control of architecture, while creating a conducive environment for cell growth and tissue formation. These findings also highlighted the potential of the proposed LBB system to advance musculoskeletal tissue engineering for regenerative medicine applications.

Conductive collagen/polypyrrole-b-polycaprolactone hydrogel for bioprinting of neural tissue constructs.

Bioprinting is increasingly being used for fabrication of engineered tissues for regenerative medicine, drug testing, and other biomedical applications. The success of this technology lies with the development of suitable bioinks and hydrogels that are specific to the intended tissue application. For applications such as neural tissue engineering, conductivity plays an important role in determining the neural differentiation and neural tissue regeneration. Although several conductive hydrogels based on metal nanoparticles (NPs) such as gold and silver, carbon-based materials such as graphene and carbon nanotubes and conducting polymers such as polypyrrole (PPy) and polyaniline were used, they possess several disadvantages. The long-term cytotoxicity of metal nanoparticles (NPs) and carbon-based materials restricts their use in regenerative medicine. The conductive polymers, on the other hand, are non-biodegradable and possess weak mechanical properties limiting their printability into three-dimensional constructs. The aim of this study is to develop a biodegradable, conductive, and printable hydrogel based on collagen and a block copolymer of PPy and polycaprolactone (PCL) (PPy-block-poly(caprolactone) [PPy-b-PCL]) for bioprinting of neural tissue constructs. The printability, including the influence of the printing speed and material flow rate on the printed fiber width; rheological properties; and cytotoxicity of these hydrogels were studied. The results prove that the collagen/PPy-b-PCL hydrogels possessed better printability and biocompatibility. Thus, the collagen/PPy-b-PCL hydrogels reported this study has the potential to be used in the bioprinting of neural tissue constructs for the repair of damaged neural tissues and drug testing or precision medicine applications.

The treatment strategies for the repair of intervertebral disc degeneration

The traditional treatment of intervertebral disc degeneration (IVDD) mainly focuses on symptomatic treatment, and cannot restore the physiological structure and function of the intervertebral disc. Therefore, more and more scholars begin to pay attention to the application of regenerative medicine and its derived therapeutic methods in IVDD. From the histological perspective, the early stage of IVDD shows the imbalance between synthesis and catabolism, but the cell number and tissue structure are relatively complete, and the intervention of exogenous molecules or gene therapy can achieve ECM regeneration. With the progress of IVDD, the replenishment of healthy cells is the key to treatment. In the final stage, the cell number and tissue structure are disordered. Biological materials with certain mechanical strength and cell load can be used to supplement ECM and healthy cells to realize the repair and regeneration of IVDD. Molecular, cell and gene therapy, combined with the application of new biomaterials, the treatment of IVDD is more inclined to compensate for the shortcomings through a combination approach in the future, in order to achieve the purpose of repair and regeneration.

Multilevel neurium-mimetic individualized graft via additive manufacturing for efficient tissue repair

Complicated peripheral nerve injuries or defects, especially at branching sites, remain a prominent clinical challenge after the application of different treatment strategies. Current nerve grafts fail to match the expected shape and size for delicate and precise branched nerve repair on a case-by-case basis, and there is a lack of geometrical and microscale regenerative navigation. In this study, we develop a sugar painting-inspired individualized multilevel epi-/peri-/endoneurium-mimetic device (SpinMed) to customize natural cues, featuring a selectively protective outer sheath and an instructive core, to support rapid vascular reconstruction and consequent efficient neurite extension along the defect area. The biomimetic perineurium dictates host-guest crosslinking in which new vessels secrete multimerin 1 binding to the fibroin filler surface as an anchor, contributing to the biological endoneurium that promotes Schwann cell homing and remyelination. SpinMed implantation into rat sciatic nerve defects yields a satisfactory outcome in terms of structural reconstruction, with sensory and locomotive function restoration. We further customize SpinMed grafts based on anatomy and digital imaging, achieving rapid repair of the nerve trunk and branches superior to that achieved by autografts and decellularized grafts in a specific beagle nerve defect model, with reliable biosafety. Overall, this intelligent art-inspired biomimetic design offers a facile way to customize sophisticated high-performance nerve grafts and holds great potential for application in translational regenerative medicine.

Three-Dimensional Bioprinting: A Comprehensive Review for Applications in Tissue Engineering and Regenerative Medicine

Since three-dimensional (3D) bioprinting has emerged, it has continuously to evolved as a revolutionary technology in surgery, offering new paradigms for reconstructive and regenerative medical applications. This review highlights the integration of 3D printing, specifically bioprinting, across several surgical disciplines over the last five years. The methods employed encompass a review of recent literature focusing on innovations and applications of 3D-bioprinted tissues and/or organs. The findings reveal significant advances in the creation of complex, customized, multi-tissue constructs that mimic natural tissue characteristics, which are crucial for surgical interventions and patient-specific treatments. Despite the technological advances, the paper introduces and discusses several challenges that remain, such as the vascularization of bioprinted tissues, integration with the host tissue, and the long-term viability of bioprinted organs. The review concludes that while 3D bioprinting holds substantial promise for transforming surgical practices and enhancing patient outcomes, ongoing research, development, and a clear regulatory framework are essential to fully realize potential future clinical applications.

Mechanobiological Strategies to Enhance Ovine (Ovis aries) Adipose-Derived Stem Cells Tendon Plasticity for Regenerative Medicine and Tissue Engineering Applications

Mechanobiological Strategies to Enhance Ovine (Ovis aries) Adipose-Derived Stem Cells Tendon Plasticity for Regenerative Medicine and Tissue Engineering Applications

Extrusion-Based Bioprinting in a Cost-Effective Bioprinter

Three-dimensional (3D) bioprinting has emerged as a revolutionary approach in the life sciences, combining multiple disciplines such as computer engineering, materials science, robotics, and biomedical engineering. This innovative technology enables the production of cellular constructs using bio-inks, and differs from conventional 3D printing by incorporating living cells. The present work addresses the conversion of a commercial thermoplastic 3D printer into a low-cost bioprinter. The modification addresses the challenges of the high cost of commercial bioprinters, limited adaptability, and specialized personnel requirements. This modification uses an extrusion-based bioprinting method that is particularly popular in research due to its viscosity tolerance and versatility. The individual steps, including replacing the extruder with a syringe pump, rebuilding the electronic motherboard, and configuring the firmware, are explained in detail. The work aims at providing access to bioprinting technology so that laboratories with modest resources can take advantage of the immense potential of this technology. This modification resulted in improved resolution, allowing submicron movements, which is comparable to some of the commercially available bioprinters. The accuracy of the modified printer was validated using hydrogel bioprinting tests, suggesting that it is suitable for broader applications in regenerative medicine.

Strategies To Enhance Biocompatibility of Bone Scaffold for Tissue Engineering Applications

Tissue engineering (TE) is a modern approach to improve or restore tissues that has been diseased or damaged by combining the factors in growth signaling and appropriate cells to form compatible biomaterial scaffold. Bone repair has been a major global health challenge in recent decades. Bone transplantation is widely used as an effective clinical treatment for this purpose. However, there are several serious issues with transplantation, including a shortage of autologous bone, immune rejection, the risk of virus transmission with allogeneic bone, and postoperative complications. In recent years, scaffolds for bone tissue engineering have emerged as a promising alternative for bone repair. These scaffolds have porous structures that mimic the extracellular matrix, which can enhance the migration, proliferation, and differentiation of osteoblasts. This process accelerates the repair of bone defects.The usage of bioactive materials has become important tool in tissue engineering and regenerative medicine application as they are to mimic tissues mechanically, chemically, and physically. Polymeric scaffold provides numerous advantageous for tissue engineering since the physicochemical properties such as porosity, pore size, solubility and biocompatibility can be controlled. Therefore, research works were carried out to explore the potential of various materials in producing bone scaffolds in tissue engineering technology. As for both newcomers and experts, this review paper will help them to find information on various types of biomaterials in imparting or enhancing their biological properties such as biocompatibility, bacterial inhibition, tissue regeneration and cell growth, bio inertness, bioactive and resorbability for bone scaffold tissue engineering application.

Magnetic cell targeting for cardiovascular tissue engineering.

There is a critical need for novel approaches to translate cell therapy and regenerative medicine to clinical practice. Magnetic cell targeting with site-specificity has started to open avenues in these fields as a potential therapeutic platform. Magnetic targeting is gaining popularity in the field of biomedicine due to its ability to concentrate and retain at a target site while minimizing deleterious effects at off-target sites. It is regarded as a relatively straightforward and safe approach for a wide range of therapeutic applications. This review discusses the latest advancements and approaches in magnetic cell targeting using endocytosed and antibody-conjugated magnetic nanoparticles as well as in vivo tracking using MRI. The most common form of magnetic particles is superparamagnetic iron-oxide nanoparticles (SPION). The biodegradable and biocompatible properties of these magnetically responsive particles and capacity for rapid endocytosis into cells makes them a breakthrough in targeted therapy. This review further discusses specific applications of magnetic targeting approaches including myocardial regeneration, endothelization of implantable cardiovascular devices and other regenerative medicine applications.

Exosomes Exposed: Overview Systematic Review on Evidence Versus Expectation in Aesthetic and Regenerative Medicine.

Exosomes, diminutive extracellular vesicles, are integral to intercellular communication, harbouring potential for applications in regenerative medicine and aesthetic interventions. The field, however, grapples with the complexities of harmonising exosome characterisation protocols and safeguarding therapeutic integrity. In this scholarly overview, systematic adherence to the Cochrane Collaboration and Preferred Reporting Items for Overviews of Reviews guidelines was observed, scrutinising the congruence of exosome-related therapies with the Minimal Information for Studies of Extracellular Vesicles standards delineated by the International Society for Extracellular Vesicles, alongside criteria set forth by the International Society for Cell Therapy and the International Society for Stem Cell Research. A meticulous search strategy spanning databases such as PubMed, Scopus, Web of Science, EMBASE, and Cochrane database was employed to encapsulate studies pertinent to the isolation, characterisation, and functional assessment of exosomes. The initial search yielded 225 articles, of which 17 systematic reviews were selected based on predefined criteria, encompassing 556 primary studies. Notwithstanding the acknowledged therapeutic promise of exosome modalities, the synthesis illuminated a prevalent deficiency in adherence to established reporting and experimental benchmarks, notably in exosome source characterisation and bioactive constituent delineation. A critical appraisal employing the AMSTAR-2 tool underscored a pervasive shortfall in methodological rigour. This review accentuates the imperative for stringent methodological standardisation within exosome research to fortify the validity and reproducibility of empirical findings. Amidst the burgeoning therapeutic optimism, the discipline must rectify methodological disparities and comply with regulatory mandates, ensuring the ethically sound and scientifically robust advancement of exosome-based therapeutic modalities. This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .

Tunable Viscoelasticity of Alginate Hydrogels via Serial Autoclaving.

Alginate hydrogels are widely used as biomaterials for cell culture and tissue engineering due to their biocompatibility and tunable mechanical properties. Reducing alginate molecular weight is an effective strategy for modulating hydrogel viscoelasticity and stress relaxation behavior, which can significantly impact cell spreading and fate. However, current methods like gamma irradiation to produce low molecular weight alginates suffer from high cost and limited accessibility. Here, a facile and cost-effective approach to reduce alginate molecular weight in a highly controlled manner using serial autoclaving is presented. Increasing the number of autoclave cycles results in proportional reductions in intrinsic viscosity, hydrodynamic radius, and molecular weight of the polymer while maintaining its chemical composition. Hydrogels fabricated from mixtures of the autoclaved alginates exhibit tunable mechanical properties, with inclusion of lower molecular weight alginate leading to softer gels with faster stress relaxation behaviors. The method is demonstrated by establishing how viscoelastic relaxation affects the spreading of encapsulated fibroblasts and glioblastoma cells. Results establish repetitive autoclaving as an easily accessible technique to generate alginates with a range of molecular weights and to control the viscoelastic properties of alginate hydrogels, and demonstrate utility across applications in mechanobiology, tissue engineering, and regenerative medicine.

BMP-Binding Polysulfonate Brushes to Control Growth Factor Presentation and Regulate Matrix Remodelling.

Bone morphogenetic proteins (BMPs) are important targets to incorporate in biomaterial scaffolds to orchestrate tissue repair. Glycosaminoglycans (GAGs) such as heparin allow the capture of BMPs and their retention at the surface of biomaterials at safe concentrations. Although heparin has strong affinities for BMP2 and BMP4, two important types of growth factors regulating bone and tissue repair, it remains difficult to embed stably at the surface of a broad range of biomaterials and degrades rapidly in vitro and in vivo. In this report, biomimetic poly(sulfopropyl methacrylate) (PSPMA) brushes are proposed as sulfated GAG mimetic interfaces for the stable capture of BMPs. The growth of PSPMA brushes via a surface-initiated activator regenerated by electron transfer polymerization is investigated via ellipsometry, prior to characterization of swelling and surface chemistry via X-ray photoelectron spectroscopy and Fourier transform infrared. The capacity of PSPMA brushes to bind BMP2 and BMP4 is then characterized via surface plasmon resonance. BMP2 is found to anchor particularly stably and at high density at the surface of PSPMA brushes, and a strong impact of the brush architecture on binding capacity is observed. These results are further confirmed using a quartz crystal microbalance with dissipation monitoring, providing some insights into the mode of adsorption of BMPs at the surface of PSPMA brushes. Primary adsorption of BMP2, with relatively little infiltration, is observed on thick dense brushes, implying that this growth factor should be accessible for further binding of corresponding cell membrane receptors. Finally, to demonstrate the impact of PSPMA brushes for BMP2 capture, dermal fibroblasts were then cultured at the surface of functionalized PSPMA brushes. The presence of BMP2 and the architecture of the brush are found to have a significant impact on matrix deposition at the corresponding interfaces. Therefore, PSPMA brushes emerge as attractive coatings for scaffold engineering and stable capture of BMP2 for regenerative medicine applications.

Ontogeny of Skin Stem Cells and Molecular Underpinnings

Skin stem cells (SCs) play a pivotal role in supporting tissue homeostasis. Several types of SCs are responsible for maintaining and regenerating skin tissue. These include bulge SCs and others residing in the interfollicular epidermis, infundibulum, isthmus, sebaceous glands, and sweat glands. The emergence of skin SCs commences during embryogenesis, where multipotent SCs arise from various precursor populations. These early events set the foundation for the diverse pool of SCs that will reside in the adult skin, ready to respond to tissue repair and regeneration demands. A network of molecular cues regulates skin SC behavior, balancing quiescence, self-renewal, and differentiation. The disruption of this delicate equilibrium can lead to SC exhaustion, impaired wound healing, and pathological conditions such as skin cancer. The present review explores the intricate mechanisms governing the development, activation, and differentiation of skin SCs, shedding light on the molecular signaling pathways that drive their fate decisions and skin homeostasis. Unraveling the complexities of these molecular drivers not only enhances our fundamental knowledge of skin biology but also holds promise for developing novel strategies to modulate skin SC fate for regenerative medicine applications, ultimately benefiting patients with skin disorders and injuries.

Directed Differentiation of Adipose-Derived Stem Cells Using Imprinted Cell-Like Topographies as a Growth Factor-Free Approach.

The influence of surface topography on stem cell behavior and differentiation has garnered significant attention in regenerative medicine and tissue engineering. The cell-imprinting method has been introduced as a promising approach to mimic the geometry and topography of cells. The cell-imprinted substrates are designed to replicate the topographies and dimensions of target cells, enabling tailored interactions that promote the differentiation of stem cells towards desired specialized cell types. In fact, by replicating the size and shape of cells, biomimetic substrates provide physical cues that profoundly impact stem cell differentiation. These cues play a pivotal role in directing cell morphology, cytoskeletal organization, and gene expression, ultimately influencing lineage commitment. The biomimetic substrates' ability to emulate the native cellular microenvironment supports the creation of platforms capable of steering stem cell fate with high precision. This review discusses the role of mechanical factors that impact stem cell fate. It also provides an overview of the design and fabrication principles of cell-imprinted substrates. Furthermore, the paper delves into the use of cell-imprinted polydimethylsiloxane (PDMS) substrates to direct adipose-derived stem cells (ADSCs) differentiation into a variety of specialized cells for tissue engineering and regenerative medicine applications. Additionally, the review discusses the limitations of cell-imprinted PDMS substrates and highlights the efforts made to overcome these limitations.

Rapid and Controllable Multilayer Cell Sheet Assembly via Biodegradable Nanochannel Membranes

AbstractThe ability to precisely arrange and control the assembly of diverse cell types into intricate 3D structures remains a critical challenge in tissue engineering. Herein, a versatile and programmable 3D cell sheet assembly is described technology by developing a biodegradable nanochannel (BNC) membrane to fulfill this unmet need. This membrane, hierarchically assembled from 2D nanomaterial aggregates, exhibits both exceptional fluid permeability and rapid biodegradation under physiological conditions. The unique properties of the BNC membrane enable precise spatial and temporal control over cell assembly, facilitating the creation of complex 3D cellular architectures. The BNC membrane is integrated with a programmable negative‐pressure‐based cell assembly strategy to form single and multicellular 3D sheets in a highly controllable manner. To demonstrate the feasibility and translatability of this technology in the field of tissue engineering approaches to screen stem cell‐derived therapeutics with “core–shell” macrophage‐fibroblast multicellular patterns and treat murine diabetic skin wounds via scaffold‐free 3D adipose‐derived mesenchymal stem cell (ADMSC) sheets are devised. In summary, the results demonstrate that the BNC membrane‐based 3D cell sheet assembly approach significantly advances current tissue engineering capabilities, offering substantial potential for both regenerative medicine applications and the development of physiologically relevant disease models.

Sphingolipidomic profiling of human Dental Pulp Stem Cells undergoing osteogenic differentiation

It is now recognized that sphingolipids are involved in the regulation and pathophysiology of several cellular processes such as proliferation, migration, and survival. Growing evidence also implicates them in regulating the behaviour of stem cells, the use of which is increasingly finding application in regenerative medicine. A shotgun lipidomic study was undertaken to determine whether sphingolipid biomarkers exist that can regulate the proliferation and osteogenic differentiation of human Dental Pulp Stem Cells (hDPSCs). Sphingolipids were extracted and identified by direct infusion into an electrospray mass spectrometer. By using cells cultured in osteogenic medium and in medium free of osteogenic stimuli, as a control, we analyzed and compared the SPLs profiles. Both cellular systems were treated at different times (72 hours, 7 days, and 14 days) to highlight any changes in the sphingolipidomic profiles in the subsequent phases of the differentiation process. Signals from sphingolipid species demonstrating clear differences were selected, their relative abundance was determined, and statistical differences were analyzed. Thus, our work suggests a connection between sphingolipid metabolism and hDPSC osteogenic differentiation and provides new biomarkers for improving hDPSC-based orthopaedic regenerative medicine.

Thermal, surface, and structure analysis of molybdenum substituted bioactive glass ceramics SiO2-CaO- MoO3-P2O5

This study explores the incorporation of molybdenum oxide (MoO3) as a dopant in SiO2-CaO-P2O5 (BGC) glass-ceramics through a meticulously designed sol-gel synthesis process to optimize properties for regenerative medicine applications. Comprehensive characterization techniques, including thermal analysis (TGA/DSC), Raman spectroscopy, scanning electron microscopy (SEM), X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy (FTIR), elucidate the morphological and structural modifications induced by MoO3 doping. Results reveal that MoO3 affects the glass transition temperature, crystallization behavior, and surface morphology, leading to enhanced glass stability, crystallinity, and surface properties. The mechanical properties, such as fracture toughness, also significantly improved with MoO3 doping due to the formation of crystalline phases and densification of the material structure. Notably, the doping of MoO3 promotes the formation of CaMoO4 crystalline phases and influences the synthesis of hydroxyapatite—critical for bone tissue regeneration—when immersed in simulated body fluid (SBF). The study highlights the hydrophilic nature of the doped samples, facilitating bone development and cell adhesion, while also showing that increased MoO3 content reduces the rate of hydroxyapatite formation and material degradability. These findings demonstrate that MoO3 doping via the sol-gel method offers a promising approach for tailoring BGC glass-ceramics' properties, making them more suitable for regenerative medicine applications. This research underscores the potential of MoO3 in advancing biomaterial development for tissue engineering and implantology.