The present research study was conducted at the experimental field condition of Pakistan Agricultural Research Council, Southern Zone Agricultural Research Centre, University of Karachi, to compare the field performance of different neem oil derivatives doses with Imidacloprid, sprayed on okra crop against the jassid population. The experiments were carried out RCBD with the application of four treatments, including three different concentrations of neem oils at (2%, 1.5%, and 1%) with the comparison of Imidacloprid pesticide and control. Neem oil was selected as a natural pesticide against jassid population reduction on okra crop. All the treatments were sprayed twice and data were recorded before 24h of pre-treatment and post-treatment at 48h, 72h, and 144h, respectively. The overall maximum reduction % after the application of Imidacloprid against the jassid population was calculated (61.51%) in 1st spray and (58.25%) 2nd spray, followed by (43.01%), (39.75%) at 2%, consequent to (32.50%), (29.75%), at 1.5% and (25.55%), (24.52%) at 1%, respectively. The maximum efficacy in neem oil concentration was recorded at 2% followed by 1.5%, and 1% but all neem oil concentrations including Imidacloprid observed effective from the 1st day up to the 6th post-spray days. All neem oil concentrations after 24h, 48h, 72h, and 144h of post-spray found significantly different at (P< 0.05). The application of neem oil, 2% caused the phytotoxicity reduction in okra plants. Thus, it is recommended that the bio-pesticide should be applied at 1.5% neem oil concentration against the jassid pest population.