Application Of BA Research Articles

Effect of Plant Growth Regulators on Growth and Yield of Sweet Pepper

A field experiment was carried out to study the effect of plant growth regulators on growth and yield of sweet pepper (cv Indra) in Rayagada district of Southern Odisha during September-January of 2019-20 and 2020-21. The experiment was laid out in Randomized Block Design with three replications and seven treatments. From the present study it was revealed that the maximum plant height (43.3 cm) was obtained with IAA (100 ppm) whereas maximum fruiting branches (8.20), maximum fruit weight (106.80 g) was obtained with 2,4-D (10 ppm). Maximum polar diameter (6.6 cm), shoulder diameter (5.2 cm) and single fruit weight (82.3 g) was obtained with BA (5 ppm). Maximum fruit yield (13.8 t/ha) was recorded with BA (5 ppm). Early fruit fetches higher market price than late harvested fruit and gives higher return to farmer which was obtained by application of BA (5 ppm) i.e., 28.1 days for first flowering and 60.2 days for first harvesting.

GLABROUS INFLORESCENCE STEMS3 binds to and activates RHD2 and RHD4 genes to promote root hair elongation in Arabidopsis.

Root hairs are crucial in the uptake of essential nutrients and water in plants. This study showed that a zinc finger protein, GIS3 is involved in root hair growth in Arabidopsis. The loss-of-function gis3 and GIS3 RNAi transgenic line exhibited a significant reduction in root hairs compared to the wild type. The application of 1-aminocyclopropane-1-carboxylic acid (ACC), an exogenous ethylene precursor, and 6-benzyl amino purine (BA), a synthetic cytokinin, significantly restored the percentage of hair cells in the epidermis in gis3 and induced GIS3 expression in the wild type. More importantly, molecular and genetic studies revealed that GIS3 acts upstream of ROOT HAIR DEFECTIVE 2 (RHD2) and RHD4 by binding to their promoters. Furthermore, exogenous ACC and BA application significantly induced the expression of RHD2 and RHD4, while root hair phenotype of rhd2-1, rhd4-1, and rhd4-3 was insensitive to ACC and BA treatment. We can therefore conclude that GIS3 modulates root hair development by directly regulating RHD2 and RHD4 expression through ethylene and cytokinin signals in Arabidopsis.

Experts’ perspectives on the sustainability and risks of freely applicable MSWI bottom ash: a Q-methodology study in the Netherlands

Experts in the Netherlands have lately debated the novel policy idea to freely apply municipal solid waste incineration bottom ash (MSWIBA). In this paper, we map this ambivalent and unforeseeable, subjective, expert debate. This will help policymaking because more knowledge on subjective expert viewpoints and perceptions allows for clustering conflict and consent as well as gaps in common understanding in this complex debate. We applied Q-methodology which resulted in four distinct perspectives in the expert debate that reveal insights into the social licence to operate and into the legitimacy of the novel bottom ash regime. The freely applicable quality of BA itself is accepted in all perspectives. Conflicting views were found about new risks, trust and socio-political acceptance of the novel BA applications. An important practical contribution of this study is that a higher acceptance of the freely applicable MSWI BA quality in the Netherlands within the expert community can be achieved if the new risks of the free application regime are tackled. We are the first to use Q-methodology in this field, and our academic contribution is that we show that this method can be a helpful tool to unravel complex expert debates also related to MSWI BA applications.

Evaluating the effect of gamma rays on Zamiifolia (Zamioculcas zamiifolia) plant in vitro and genetic diversity of the resulting genotypes using the ISSR marker

Zamiifolia (Zamioculcas sp.) is a perennial plant in the Araceae family and one of the new apartment plants in the world. In this study, in order to increase the effectiveness of the breeding program, tissue culture technique and explants of leaf parts were used. The results indicated that 2,4-D (1 mg/l) and BA (2 mg/l) hormones affected positively and significantly callus formation and simultaneous application of NAA and BA (both in 0.5 mg/l) caused the best results regarding seedling production and number, leaves, complete tubers, and root in tissue culture of Zaamifolia. In the study, three cultivars of Zamiifolia (green, black and Dutch) and 12 genotypes resulted from callus formation stage, irradiated with different gamma rays (0 to 175 Gy, with LD50 as 68 Gy) were selected and the presence of genetic diversity was investigated using 22 ISSR primers. Applying ISSR marker showed that the highest amount of PIC values related to the F19(0.47) and F20(0.38) primers, which persuasively isolated the studied genotypes. Moreover, the highest efficiency was detected for AK66 marker based on the MI parameter. The PCA and clustering categorization via UPGMA methodology based on molecular information and Dice index, differentiated the genotypes into 6 groups. Genotypes 1(callus), 2(100 Gy) and 3(cultivar from Holland) created separated groups. The 4th group included 6(callus), 8(0 Gy), 9(75 Gy), 11(90 Gy), 12(100 Gy) and 13(120 Gy) genotypes appearing as the largest group. The 5th group included 7(160 Gy), 10(80 Gy), 14(140 Gy) and 15(Zanziber gem black) genotypes. The last group included 4(mather plant) and 5(callus) genotypes. In this context, genotypes 1, 5, and 6 had probably somaclonal variation. Moreover, genotypes that received doses of 100 and 120 Gy, had a medium diversity. There is a high possibility of introducing a cultivar with a low dose and high genetic diversity in the whole group. Genotype 7 in this classification, received the highest dose of 160 Gy. In this population, the Dutch variety, was used as a new variety. As a result, the ISSR marker could correctly group the genotypes. This is an interesting finding, and it could be hypothesized that the ISSR marker could correctly differentiate Zaamifolia genotypes and probably other ornamental plants under the effect of gamma rays mutagenesis in order to achieve novel variants.

Effect of Foliar Spraying of Gibberellins and Brassinolide on Cadmium Accumulation in Rice.

Cadmium (Cd) is one of the heavy metals that contaminate rice cultivation, and reducing Cd contamination in rice through agronomic measures is a hot research topic. In this study, foliar sprays of gibberellins (GA) and brassinolide (BR) were applied to rice under Cd stress in hydroponic and pot experiments. After foliar spraying of GR and BR, the biomass of rice plants grown in either hydroponics or soil culture was significantly higher or even exceeded that in the absence of Cd stress. In addition, photosynthetic parameters (maximum fluorescence values), root length and root surface area, and CAT, SOD and POD activities were significantly improved. The MDA content decreased in the shoots, suggesting that the application of GR and BA may have enhanced photosynthesis and antioxidant function to alleviate Cd stress. Furthermore, the BR and GA treatments decreased the Cd content of rice roots, shoots and grains as well as the Cd transfer coefficient. Cd chemical morphology analysis of rice roots and shoots showed that the proportion of soluble Cd (Ethanol-Cd and Water-Cd) decreased, whereas the proportion of NaCl-Cd increased. Analysis of the subcellular distribution of Cd in rice roots and above ground showed that the proportion of Cd in the cell wall increased after foliar spraying of GA and BR. The results indicate that after foliar application of GA and BR, more of the Cd in rice was transformed into immobile forms and was fixed in the cell wall, thus reducing the amount in the seeds. In summary, foliar sprays of GA and BR can reduce the toxic effects of Cd on rice plants and reduce the Cd content in rice grains, with GA being more effective.

Influence of plant growth regulators on growth and formation of sylleptic shoots in one-year-old apple cv. Gala Mast

Aim: To examine the effectiveness of 6-benzyladenine (BA) alone or in combination with gibberellic acid (GA3) on sylleptic shoots formation on one-year-old apple cv. Gala Mast grafted on MM-106 rootstock. Methodology: During the second vegetative period, the actively growing apical portion of the nursery tree was sprayed with varying doses of BA and BA+GA3 (200, 300, 400, 500, 600, and 700 ppm) using a hand sprayer until run-off. Three spray treatments were performed at one week apart. The first spray was applied when the growth of the central leader reached 70-80 cm. Results: All plant growth regulators treatments significantly increased the number and length of sylleptic shoots, branching zone and per cent branched trees compared to control. The unsprayed trees had an average of 1.95 useful sylleptic per tree, 1.1 m total sylleptic shoot length, 10.24 cm branching zone and 35 per cent branched trees. Application of BA (700 ppm) resulted in four-fold increase in the number of sylleptic shoots (7.8), more than two-fold increase in total length of sylleptic shoots (2.44 m), and around four-fold increase in branching zone (38.96 cm). The 100 per cent branched trees were obtained with the application of BA (500, 600, 700 ppm) and BA+GA3 (600, 700 ppm) in both experimental years. Moreover, these treatments obtained 2.78 to 2.90 values of the trunk and mean sylleptic shoot diameter ratio. Interpretation: Application of BA alone or in combination with GA3 played an important role in overcoming apical dominance and therefore in the production of well-branched apple nursery tree. However, application of BA alone has a stronger positive effect on sylleptic shoot formation. More than 400 ppm BA appears to give a significant stimulation for the production of sylleptic shoots, with the highest beneficial effect reported with the application of 700 ppm BA. However, it is cost-effective to apply 3 sprays of 500 ppm BA for adequate growth and sylleptic shoot production. Key words: 6-benzyladenine, Gibberellic acid, Nursery tree, Plant growth regulators, Sylleptic shoot

Planting Density and Application of BA for the Production of Well-feathered Bi-axis Apple Trees

Planting Density and Application of BA for the Production of Well-feathered Bi-axis Apple Trees

Effects of Plant Growth Regulators on Plum (Prunus domestica L.) Grown on Two Rootstocks at Harvest and at the Postharvest Period

Plant growth regulators (PGRs), such as cytokinins (6-benzyladenine; BA) and gibberellins (GAs), are widely used in fruit production. This study focused on the plum cultivar “Čačanska rodna” (P. domestica L.) grafted on vegetative rootstock “WaVit” and generative rootstock Prunus cerasifera, with interstock Prunus spinosa. PGR treatments included 50 mg L−1 and 100 mg L−1 of BA and 200 mg L−1 of gibberellin A3 (GA3) and non-treated control. Pomological characteristics of plum fruits were examined at harvest, while physicochemical properties were analyzed at harvest and after 28 days of cold storage and 4 days of shelf life. GA3 and BA application changed the fruit morphological traits, improved skin strength, and increased carotenoid, anthocyanin and sucrose content while decreasing the titratable acidity at harvest. The beneficial effects of higher sucrose, anthocyanin and carotenoid levels persisted in all PGR-treated fruits after cold storage and shelf life. GA3-treated fruits had firmer flesh, stronger skin and higher total soluble solids (TSS) content, while in BA-treated plums, these effects were rootstock-dependent. The physical properties and chemical composition of plum fruit in the postharvest period suggest beneficial effects of the applied PGR treatments. Moreover, these chemical treatments might have prolonged the beneficial impact on fruit storability, nutritional profile and sensory properties. Based on our results, GA3 preharvest treatment can be included in standard cultivation practices within contemporary production systems of European plums not only to improve fruit quality at harvest but also to improve the storage potential and nutritional value, regardless of the rootstock used.

Chemical thinning induced by single and sequential application of BA, NAA and metamitron on ‘Gala’ and ‘Golden Delicious’ apple

Chemical thinning induced by single and sequential application of BA, NAA and metamitron on ‘Gala’ and ‘Golden Delicious’ apple

Early Sucrose Degradation and the Dominant Sucrose Cleavage Pattern Influence Lycoris sprengeri Bulblet Regeneration In Vitro.

Bulblet formation and development determine the quantitative and qualitative traits, respectively, of bulb yield for most flowering bulbs. For Lycoris species, however, the underlying molecular mechanism remains elusive. Here, clonal bulblets of Lycoris sprengeri (Ls) derived from the same probulb were used as explants to establish efficient and inefficient in vitro regeneration systems by adjusting the 6-benzyladenine (BA) concentrations in media. BA application did not change the biological processes among groups but led to earlier decreases in sucrose and total soluble sugar (TSS) contents. Correlation analyses showed that the BA treatments changed the interaction between carbohydrate and endogenous hormone contents during bulblet regeneration. We found that two sucrose degradation enzyme-related genes, cell wall invertase (CWIN) and sucrose synthase, exhibited exactly opposite expression patterns during the competence stage. In addition, the regeneration system that obtained more bulblets showed significantly higher expression of LsCWIN2 than those that obtained fewer bulblets. Our data demonstrate the essential role of BA in accelerating sucrose degradation and the selection of a dominant sucrose cleavage pattern at the competence stage of in vitro bulblet regeneration. We propose that a relatively active CWIN-catalyzed pathway at the competence stage might promote bulblet regeneration, thus influencing bulb yield.

Assessment of In-vitro culture through Nodal explants of Dendrocalamus hamiltonii

The present experimental study was aimed to overcome the traditional methods of propagation that limit the number of propagules by in-vitro regeneration through nodal explants of Dendrocalamus hamiltonii with a comparative study of growth regulators during the shooting and rooting process. Dendrocalamus hamiltonii is distributed from the Himalayas (Nepal) to the northern part of Burma. Collection of explants was done from different selected sites of CPTs. There was the use of HgCl2 and Ca (OCl)2 as sterilizing agents in different concentrations and its effect was visualized during the sprouting stage. Culm explants were cultured in a bottle containing White media (Wm) supplemented with BA and Kinetin for sprouting and IAA, IBA, NAA for rooting. There is also the use of IAA+IBA+NAA in combined form as a supplementary solution 0.1% HgCl2 treatment for 20-minute results into77.80% aseptic buds and 72% bud -break. Among the used growth-hormones, BA with concentration 0.25mg/l and 0.50mg/l respectively were appropriate for shoot-multiplication rate, 4.01±0.3 and 4.3±0.4 were ideal observation incorporation with BA (1.00mg/l) and BA (1.50mg/l) respectively. Maximum sprouting rate14.77±3.37with application of BA (2.00mg/l) and maximum shoot length4.3±0.4 is observed at BA (1.50mg/l). The applications of rooting hormone IAA+IBA+NAA in the concentration of 1.0 mg/l results in 72.5±0.3(rooting) and 11.1±0.3 (av. No. of the root).

Foliar Application of Auxin or Cytokinin Can Confer Salinity Stress Tolerance in Vicia faba L.

Soil salinity severely declines the availability of water and essential minerals to the plants, which hinders growth. The present study evaluates the potential roles of indole-3-acetic acid (IAA) and 6-benzyladenine (BA) for mitigating the adverse effects of soil-salinity in faba bean (Vicia faba L.). Plants were exposed to 150 mM NaCl stress and were sprayed with IAA (1.15 mM) or BA (0.9 mM). Our results revealed that foliar application of IAA or BA improved the growth traits of salinized faba bean due to the increased uptake of K+, Ca2+, and Mg2+ ions, accumulation of free amino acids, soluble sugars, and soluble proteins, and activity of superoxide dismutase, catalase, peroxidase, and ascorbate peroxidase. The principal component analysis (PCA) and heatmap clustering indicated that salinity-exposed plants exhibited lower growth and biomass production, which correlated with higher accumulation of Na+ and malondialdehyde. Moreover, electrophoretic patterns of protein showed new bands in IAA- or BA-treated salt-stressed plants, indicating that IAA or BA treatment can reprogram the metabolic processes to confer salinity tolerance. We also found that IAA has a greater capacity to ameliorate the salt stress than BA, although there is no significant difference in yield between these treatments. Finally, these findings can be helpful for a better understanding of IAA- and BA-mediated salt tolerance mechanisms and increasing production of faba bean in saline soils.

Mitigation of salinity stress by exogenous application of cytokinin in faba bean (Vicia faba L.)

Soil salinity limits agricultural land use and crop productivity, thereby a major threat to global food safety. Plants treated with several phytohormones including cytokinins were recently proved as a powerful tool to enhance plant’s adaptation against various abiotic stresses. The current study was designed to investigate the potential role of 6-benzyladenine (BA) to improve broad bean (Vicia faba L.) salinity tolerance. The salt-stressed broad bean plantlets were classified into two groups, one of which was sprayed with water and another was sprayed with 200 ppm of BA. Foliar applications of BA to salt-exposed plants promoted the growth performance which was evidenced by enhanced root-shoot fresh and dry biomass. Reduced proline was strongly connected to the enhanced soluble proteins and free amino acids contents, protecting plant osmotic potential following BA treatment in salt-stressed broad bean. BA balanced entire mineral homeostasis and improved mineral absorption and translocation from roots to shoots, shoots to seeds and roots to seeds in salt-stressed plants. Excessive salt accumulation increased malondialdehyde level in leaves creating oxidative stress and disrupting cell membrane whereas BA supplementation reduced lipid peroxidation and improved oxidative defence. BA spray to salinity-stressed plants also compensated oxidative damage by boosting antioxidants defence mechanisms, as increased the enzymatic activity of superoxide dismutase, catalase, peroxidase and ascorbate peroxidase. Moreover, clustering heatmap and principal component analysis revealed that mineral imbalances, osmotic impairments and increased oxidative damage were the major contributors to salts toxicity, on the contrary, BA-augmented mineral homeostasis and higher antioxidant capacity were the reliable markers for creating salinity stress tolerance in broad bean. In conclusion, the exogenous application of BA alleviated the antagonistic effect of salinity and possessed broad bean to positively regulate the osmoprotectants, ion homeostasis, antioxidant activity and finally plant growth and yield, perhaps suggesting these easily-accessible and eco-friendly organic compounds could be powerful tools for the management of broad bean growth as well as the development of plant resiliency in saline prone soils.

Municipal solid waste incineration bottom ash fines: Transformation into a minor additional constituent for cements

Increasing amounts of waste are incinerated every year; therefore, the amount of Municipal Solid Waste Incineration Bottom Ash (MSWI BA) also increases. At the same time, limited landfill space and the goal of achieving a circular economy dictate the search for MSWI BA applications. Large fractions are successfully used to replace aggregates in concrete, but the MSWI BA fraction < 3 mm has several disadvantages, such as high porosity and high contamination that make its application as aggregate or cement replacement difficult. However, finding a tailored treatment can turn these fines into a potential filler material (Minor Additional Constituent, MAC, 5% w/w) in Portland cement. This paper investigates milled fines that were produced out of MSWI BA fines (0.125–3 mm) to match the size distribution of CEM I 52.5 R and CEM I 42.5 N. They are also compared to the untreated fines (< 0.125 mm) from the same MSWI BA. The influence of milling on the fines was investigated by SEM (morphological analysis) and the leaching test to assess the potential environmental impact. Studied by isothermal calorimetry, the effect of the designed fines on cement hydration appears to be very small and the results for the mechanical performance of mortars show that all fines examined in the study might be considered as potential MACs.

Cytokinins affect shoot regeneration and modulate the expression of IPT and CKX genes of in vitro cultured Eleocharis dulcis (Brum.f.) Trin

ABSTRACT The Chinese water chestnut (Eleocharis dulcis (Brum.f.) Trin.) is conventionally propagated by underground corm with a lower propagation rate. In the present study, we attempted to develop a simple and efficient protocol for in vitro shoot regeneration, corm formation, and the generation of plantlets, on MS medium supplemented with BA, TDZ, NAA, and IAA. Additionally, the effect of BA on the expression of EdIPT and EdCKX genes was determined. The highest number of shoots (10.9) was produced by 0.5 mg/L BA, while that was the 0.1 mg/L TDZ (11.9), followed by the combination of 0.1 mg/L TDZ and 0.5 mg/L IAA (10.6), and shoot produced root spontaneously in these treatments. 60 g/L of sucrose was optimal for inducing the shoots while the highest corm number (5.8) was achieved by 90 g/L sucrose. 1.0 mg/L BA was optimal for corm formation with the highest number of corm (3.5) and the largest diameter (7.4 mm). NAA or IAA at 0.1 mg/L was optimal for rooting. Furthermore, we found the expression of EdIPT gene was up-regulated by the application of BA at a relatively lower level, and a high concentration of BA promoted expression of EdCKX gene.

Acid invertase activities of dahlia ‘Kokucho’ petals during flower opening and following cutting and treatment with 6-benzylaminopurine

Extending the vase life of cut dahlia ‘Kokucho’ flowers through enhanced activities of acid invertases (cell wall, CWIN and vacuolar, VIN) on flower opening and senescence after harvest was investigated. The activities of CWIN and VIN were very low in the outer petals at the tight bud stage (stage 1), increased markedly at the half-open stage (stage 2), and decreased at the fully open stage (stage 3) to levels lower than at stage 2. Glucose and fructose concentrations were very low at stage 1, markedly increased at stage 2, and further increased at stage 3. These results suggest that acid invertase activities play a very important role in increasing soluble sugars of petals during flower opening. Following harvest at stage 3, florets or cut flowers were treated with 50 μmol L−1 6-benzylaminopurine (BA) by dipping or spraying, respectively. These treatments extended vase life longer than in the controls. In the florets experiment, CWIN and VIN activities were significantly higher with a BA dip than in the control for petals at 3 and 6 d, but there were no significant differences in sugar concentrations between the control and BA dip groups. In cut flowers at 3 d after cutting, VIN activity and glucose concentration were higher in the outer petals treated with BA spray than in control petals. From these results, application of exogenous cytokinin has some effect of maintaining high acid invertase activity, and high invertase activity itself may be important for delaying senescence in cut dahlia flowers. BA application is useful for extending petal longevity and is expected to be a popular post-harvest treatment in cut dahlia flowers.

Potential Use of Incineration Bottom Ash in Construction: Evaluation of the Environmental Impact

Knowledge of the long-term leaching behavior of potentially harmful substances is crucial for the assessment of the environmental compatibility of reusing municipal solid-waste incineration bottom ash (MSWI BA) in construction, i.e., as a road base layer. BA fractions obtained from wet-processing aiming at the improvement of environmental quality were used to investigate the mobility of relevant substances. Eluates from laboratory-scaled leaching procedures (column percolation and lysimeters) were analyzed to learn about the long-term release of substances. Unsaturated conditions and artificial rainwater (AR) were used in the lysimeter tests to simulate field conditions. In addition, batch test eluates were generated at usual liquid-to-solid ratios (L/S) for compliance testing purposes. A variety of cations and anions was measured in the eluates. The wet treatment reduces the leaching of chloride and particularly sulfate by more than 60%. The release of typical contaminants for the treated MSWI BA such as the heavy metals Cu and Cr was well below 1% in the conducted leaching tests. An increase in the Sb concentration observed in the lysimeter experiments starting at L/S 0.75 L/kg and in the column experiment at L/S 4 L/kg is assumed to be related to decreasing concentrations of Ca and thus to the dissolution of sparingly soluble calcium antimonate. The same leaching mechanism applies with V, but the concentration levels observed are less critical regarding relevant limit values. However, on the long term the behavior of Sb could be problematic for the application of MSWI BA in construction.Graphic

Developmental basis for flower sex determination and effects of cytokinin on sex determination in Plukenetia volubilis (Euphorbiaceae)

Key messageCytokinin might be an important factor to regulate floral sex at the very early stage of flower development in sacha inchi.Sacha inchi (Plukenetia volubilis, Euphorbiaceae) is characterized by having female and male flowers in a thyrse with particular differences. The mechanisms involved in the development of unisexual flowers are very poorly understood. In this study, the inflorescence and flower development of P. volubilis were investigated using light microscopy and scanning electron microscopy. We also investigated the effects of cytokinin on flower sex determination by exogenous application of 6-benzyladenine (BA) in P. volubilis. The floral development of P. volubilis was divided into eight stages, and the first morphological divergence between the male and female flowers was found to occur at stage 3. Both female and male flowers can be structurally distinguished by differences in the shape and size of the flower apex after sepal primordia initiation. There are no traces of gynoecia in male flowers or of androecia in female flowers. Exogenous application of BA effectively induced gynoecium primordia initiation and female flower development, especially at the early flower developmental stages. We propose that flower sex is determined earlier and probably occurs before flower initiation, either prior to or at inflorescence development due to the difference in the position of the female and male primordia in the inflorescence and in the time of the female and male primordia being initiated. The influence of cytokinin on female primordia during flower development in P. volubilis strongly suggests a feminization role for cytokinin in sex determination. These results indicate that cytokinin could modify the fate of the apical meristem of male flower and promote the formation of carpel primordia in P. volubilis.

Kinematics of chromodynamic multicomponent lattice Boltzmann simulation with a large density contrast.

The utility of an enhanced chromodynamic, color gradient or phase-field multicomponent lattice Boltzmann (MCLB) equation for immiscible fluids with a density difference was demonstrated by Wen etal. [Phys. Rev. E 100, 023301 (2019)2470-004510.1103/PhysRevE.100.023301] and Ba etal. [Phys. Rev. E 94, 023310 (2016)2470-004510.1103/PhysRevE.94.023310], who advanced earlier work by Liu etal. [Phys. Rev. E 85, 046309 (2012)PLEEE81539-375510.1103/PhysRevE.85.046309] by removing certain error terms in the momentum equations. But while these models' collision scheme has been carefully enhanced by degrees, there is, currently, no quantitative consideration in the macroscopic dynamics of the segregation scheme which is common to all. Here, by analysis of the kinetic-scale segregation rule (previously neglected when considering the continuum behavior) we derive, bound, and test the emergent kinematics of the continuum fluids' interface for this class of MCLB, concurrently demonstrating the circular relationship with-and competition between-the models' dynamics and kinematics. The analytical and numerical results we present in Sec.V confirm that, at the kinetic scale, for a range of density contrast, color is a material invariant. That is, within numerical error, the emergent interface structure is isotropic (i.e., without orientation dependence) and Galilean-invariant (i.e., without dependence on direction of motion). Numerical data further suggest that reported restrictions on the achievable density contrast in rapid flow, using chromodynamic MCLB, originate in the effect on the model's kinematics of the terms deriving from our term F_{1i} in the evolution equation, which correct its dynamics for large density differences. Taken with Ba's applications and validations, this result significantly enhances the theoretical foundation of this MCLB variant, bringing it somewhat belatedly further into line with the schemes of Inamuro etal. [J. Comput. Phys. 198, 628 (2004)JCTPAH0021-999110.1016/j.jcp.2004.01.019] and the free-energy scheme [see, e.g., Phys. Rev. E. 76, 045702(R) (2007)10.1103/PhysRevE.76.045702, and references therein] which, in contradistinction to the present scheme and perhaps wisely, postulate appropriate kinematics a priori.

Biomass Accumulation, Photosynthetic Pigments, Osmotic Adjustments and Antioxidant Activities of Leymus chinensis in Response to BA, BR, and GA

ABSTRACT: Abiotic stresses and poor biomass accumulation are chief constraints to accomplish potential yield in Leymus chinensis. An experiment was conducted to improve biomass and correlation of biomass accumulating attributes with photosynthetic pigments, osmotic substances and antioxidants under foliar application of different plant growth substances. The experiment was conducted at inner Mongolia Xilinguole, China, using a Randomized Complete Block Design and 5 replications. The treatments consisted of water (control); BA5 = application of BA (6-benzylaminopurine) at 5 mg L-1; BA25 = application of BA at 25 mg L-1; BA50 = application of BA at 50 mg L-1; BR0.02 = application of BR (brassinosteroid) at 0.02 g L-1; BR0.2 = application of BR at 0.2 mg L 1; BR2 = application of BR at 2 mg L-1; GA10 = application of GA (gibberellic acid) at 10 mg L-1; GA50 = application of GA at 50 mg L-1 and GA100 = application of GA at 100 mg L-1. Application of all plant growth substances significantly improved biomass, osmotic adjustments, photosynthetic pigments and antioxidant activities compared to control. However, the most promising results were found with 0.2 mg L-1 BR. The highest chlorophyll a/b, glutathione and ascorbate peroxidase activities were recorded with 25 mg L-1 BA. Conclusively, 25 mg L-1 BA, 0.2 mg L-1 BR and 10 mg L-1 GA exhibited more promising results than other concentrations for the evaluated attributes.