Foliar Application of Auxin or Cytokinin Can Confer Salinity Stress Tolerance in Vicia faba L.

Arafat Abdel Hamed Abdel Latef,Ayasha Akter,Md Tahjib-Ul-Arif

doi:10.3390/agronomy11040790

Arafat Abdel Hamed Abdel Latef, Ayasha Akter + Show 1 more

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https://doi.org/10.3390/agronomy11040790

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Journal: Agronomy	Publication Date: Apr 16, 2021
Citations: 24	License type: CC BY 4.0

Affiliation: Bangladesh Agricultural University, Taif University

Abstract

Soil salinity severely declines the availability of water and essential minerals to the plants, which hinders growth. The present study evaluates the potential roles of indole-3-acetic acid (IAA) and 6-benzyladenine (BA) for mitigating the adverse effects of soil-salinity in faba bean (Vicia faba L.). Plants were exposed to 150 mM NaCl stress and were sprayed with IAA (1.15 mM) or BA (0.9 mM). Our results revealed that foliar application of IAA or BA improved the growth traits of salinized faba bean due to the increased uptake of K+, Ca2+, and Mg2+ ions, accumulation of free amino acids, soluble sugars, and soluble proteins, and activity of superoxide dismutase, catalase, peroxidase, and ascorbate peroxidase. The principal component analysis (PCA) and heatmap clustering indicated that salinity-exposed plants exhibited lower growth and biomass production, which correlated with higher accumulation of Na+ and malondialdehyde. Moreover, electrophoretic patterns of protein showed new bands in IAA- or BA-treated salt-stressed plants, indicating that IAA or BA treatment can reprogram the metabolic processes to confer salinity tolerance. We also found that IAA has a greater capacity to ameliorate the salt stress than BA, although there is no significant difference in yield between these treatments. Finally, these findings can be helpful for a better understanding of IAA- and BA-mediated salt tolerance mechanisms and increasing production of faba bean in saline soils.

Highlights

Plants are often exposed to a variety of environmental stresses such as salinity, drought, and temperature that have undesirable consequences on their growth and biomass production [1]
The salt-stressed faba bean plants showed a considerable decrease in all growth parameters such as root fresh weight (RFW), root dry weight (RDW), shoot fresh weight (SFW), shoot dry weight (SDW), leaf fresh weight (LFW), leaf dry weight (LDW), Leaf area (LA), and number of nodules as well as fresh and dry yields than the non-stressed plants (Table 1)
We obtained that exogenously indole-3-acetic acid (IAA) or BA reduced MDA accumulations and improved membrane integrity in faba bean plants grown under salt stress (Figure 3A). These results suggest that IAA or BA might recover the adverse effect of faba bean plants from salt stress by reducing oxidative damage and contributed to better salinity tolerance

Summary

Introduction

Plants are often exposed to a variety of environmental stresses such as salinity, drought, and temperature that have undesirable consequences on their growth and biomass production [1]. Soil salinity is one of the devastating abiotic stresses limiting crop growth and productivity worldwide [1,2,3]. Most of the fertile lands would be affected by salt intrusion by the mid-twentieth century [9]. This is a serious issue because saline areas are expanding at a rate of 10% annually, which would be exacerbated by increased global warming and climatic changes [10]

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