Early Sucrose Degradation and the Dominant Sucrose Cleavage Pattern Influence Lycoris sprengeri Bulblet Regeneration In Vitro.

Ziming Ren,Yue Liu,Yiping Xia,Cong Gao,Yun Wu,Yefan Lin,Dong Zhang,Xuesi Lvy,Yunchen Xu

doi:10.3390/ijms222111890

Ziming Ren, Yue Liu + Show 7 more

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https://doi.org/10.3390/ijms222111890

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Abstract

Bulblet formation and development determine the quantitative and qualitative traits, respectively, of bulb yield for most flowering bulbs. For Lycoris species, however, the underlying molecular mechanism remains elusive. Here, clonal bulblets of Lycoris sprengeri (Ls) derived from the same probulb were used as explants to establish efficient and inefficient in vitro regeneration systems by adjusting the 6-benzyladenine (BA) concentrations in media. BA application did not change the biological processes among groups but led to earlier decreases in sucrose and total soluble sugar (TSS) contents. Correlation analyses showed that the BA treatments changed the interaction between carbohydrate and endogenous hormone contents during bulblet regeneration. We found that two sucrose degradation enzyme-related genes, cell wall invertase (CWIN) and sucrose synthase, exhibited exactly opposite expression patterns during the competence stage. In addition, the regeneration system that obtained more bulblets showed significantly higher expression of LsCWIN2 than those that obtained fewer bulblets. Our data demonstrate the essential role of BA in accelerating sucrose degradation and the selection of a dominant sucrose cleavage pattern at the competence stage of in vitro bulblet regeneration. We propose that a relatively active CWIN-catalyzed pathway at the competence stage might promote bulblet regeneration, thus influencing bulb yield.

Highlights

Lycoris is a monocotyledonous genus belonging to the Amaryllidaceae family and having high medicinal, ornamental and ecological value [1,2,3,4,5]
Histological Structure of Clonal Bulblets Derived In Vitro
Clonal bulblets derived from the same probulb were used as plant materials in this study (Figure S1)

Summary

Introduction

Lycoris is a monocotyledonous genus belonging to the Amaryllidaceae family and having high medicinal, ornamental and ecological value [1,2,3,4,5]. Lycoris sprengeri (Ls) is an endemic Lycoris species in China [6] that has become an important ground cover for urban landscaping in recent years [7]. This plant features vibrant, blue-purple flowers with strong resistance and low maintenance costs. The increasing demand for Ls bulbs has already resulted in significant resource diminishment and heavy exploitation of their natural habitats To address this situation, our previous research developed an effective in vitro bulblet multiplication protocol [4], providing a series of clonal bulblets derived from one individual seed, as native Lycoris from different genetic backgrounds is not suitable for current research

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