Small For Age Research Articles

Growth and Yield of Celery (Apium Graveolens L.) in Organic Cultivation on Alluvial Soil

Celery (Apium graveolens L.) is a horticultural crop that is consumable and offers numerous health benefits. The purpose of this study was to ascertain how celery growth and yield cultivated on alluvial soil were affected by the interaction between liquid organic NPK fertilizer and chicken manure. The study utilized a field experiment that was designed completely randomized comprising two factors. The first factor was the dosage of chicken manure with three levels: C1: 50 g/pot, C2: 100 g/pot, and C3: 150 g/pot. The second factor was liquid organic NPK fertilizer with three levels: L1: 1%, L2: 2%, and L3: 3%. All treatment combinations were performed in triplicates. The results indicated that the interaction between chicken manure and liquid organic NPK fertilizer significantly affected the number of tillers and dry weight of celery yield. Chicken manure as a single factor significantly influenced the number of stems and tillers. Liquid organic NPK fertilizer as a single factor significantly influenced the number of tillers, fresh weight, dry weight, and plant height. The combination of chicken manure and liquid organic NPK fertilizer (C1L3) produced the highest number of stems per clump (79.44 stems) and the highest number of tillers per clump (9.78 tillers). The combination of C2L3 yielded the highest fresh weight (184 g) and dry weight (17.33 g). Keywords: Alluvial soil, Celery, Chicken manure, Liquid organic NPK fertilizer

Ethnomedicinal survey of anti-urolithiatic medicinal plants used in the region of Mascara, Algeria

Objective: To conduct an ethnomedicinal survey and conserve traditional medicinal practices in the Mascara region of northwestern Algeria. In this region, traditional medicinal knowledge has primarily been transmitted orally, and the study aims to document it in written form for better utilization in the field of pharmacology. Methods: Purposive sampling with semi-structured questionnaires was adopted with ethnobotanical data obtained from 135 local informants, including 22 herbalists. The study participants were asked about their sociodemographic profiles and ethnomedicinal knowledge about anti-urolithiatic medicinal plants. Results: Sixty-three anti-urolithiatic species belonging to 59 genera and 32 families were inventoried. All plants are prescribed orally, and decoction presents the most common preparation method (46.0%). Seeds are the most used parts (27.0%). The most frequent botanical families were Apiaceae, Lamiaceae, Fabaceae, and Rosaceae. The predominant anti-urolithiatic plants are Hemiaria hirsute L., Hordeum vulgare L., Linum usitatissimum L., Zea mays L., Lavandula angustifolia Mill., and Apium graveolens L. Conclusions: This study highlights the diversity of the phytotherapeutic practices used against urolithiasis in the region of Mascara. It also suggests that ethnomedicine is well-entrenched in local health practices in this area. However, this knowledge acquired by the local inhabitants over the centuries risks disappearing since it is mainly held by older people and is not documented.

Comparative study of celery (Apium graveolens) on growth, yield and quality under different growing conditions

An experiment was conducted during October 2022–January 2023 at Defence Institute of Bio-energy Research-Defence Research and Development Organisation, Haldwani, Nainital, Uttarakhand to evaluate the growth, quality production and yield of celery under the hydroponic, soilless and soil conditions. Higher crop productivity of celery crop was found under hydroponic system as compared to other medium. The experiment revealed that there is a better provision for vertical space utilization and protection from soil-borne disease under hydroponic farming as compared to traditional farming. This experiment paved a new way for the need and future perspective of hydroponic technology to achieve the desirable quality of vegetable crop production by using modern farming technology such as hydroponics on a small and medium scale. The present experiment also summarized that this farming plays an important role in crop production to maintain market growth in coming years. In this respect, hydroponics is an emerging technology which play a vital role in urban and pre-urban area and positively effective in the ways to promote healthy and developed future for the planet and its people.

Safety and efficacy of a feed additive consisting of an essential oil obtained from the fruit of Apium graveolens L. (celery seed oil) for all animal species (FEFANA asbl).

Following a request from the European Commission, EFSA was asked to deliver a scientific opinion on the safety and efficacy of an essential oil obtained from the fruit of Apium graveolens L. (celery seed oil), when used as a sensory additive in feed and water for drinking for all animal species. The EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) Panel concluded that the use of celery seed oil is of no concern up to the following concentrations in complete feed: 1.6 mg/kg for chickens for fattening, 2.3 mg/kg for laying hens, 2.1 mg/kg for turkeys for fattening, 2.8 mg/kg for piglets, 3.3 mg/kg for pigs for fattening, 4.1 mg/kg for sows, 6.5 mg/kg for veal calves (milk replacer), 6.2 mg/kg for cattle for fattening, sheep, goats and horses, 4.0 mg/kg for dairy cows, 2.5 mg/kg for rabbits, 6.8 mg/kg for salmonids and 7.2 mg/kg for dogs. These conclusions were extrapolated to other physiologically related species. For cats, ornamental fish and other species, no conclusion can be drawn. The use of celery seed oil in animals feed is not expected to pose concern for the consumers and for the environment. The additive under assessment should be considered as an irritant to skin and eyes, and as a respiratory and skin sensitiser. When handling the essential oil, exposure of unprotected users to perillaldehyde and bergapten may occur. Therefore, to reduce the risk, the exposure of the users should be minimised. Since A. graveolens and its preparations were recognised to flavour food and its function in feed would be essentially the same as that in food, no further demonstration of efficacy was considered necessary.

Methanol extract from Apium graveolens stimulate glucagon-like peptide-1 secretion (GLP-1), reduced dipeptidyl-peptidase-4 (DPP-4) and protect pancreatic b cells against glucotoxicity and H2O2 toxicity

Methanol extract from Apium graveolens stimulate glucagon-like peptide-1 secretion (GLP-1), reduced dipeptidyl-peptidase-4 (DPP-4) and protect pancreatic b cells against glucotoxicity and H2O2 toxicity

Effects of Drying Temperatures and Drying Methods on Physicochemical and Antioxidant Properties of Celery Powder

ABSTRACT The Celery (Apium graveolens Linn.) were hot air dried at 60, 70, and 80°C. Drying temperatures had no significant impact on the bulk density, water holding capacity, or swelling capacity of celery powder. High drying temperatures (80°C) generated a significant color shift, whereas antioxidants were decreased at a low temperature (60°C). Thus, the temperature of 70°C was recommended because the powder had satisfying green color, high phenolic content (597.35 mg GAE/100 g), and DPPH inhibition (39.37%). Subsequently, celery samples were dried using hot air drying (at 70°C) and freeze-drying and the juice was foam mat dried. The foam mat dried powder had less water holding capacity, whereas the hot air and freeze-dried powders had similar characteristics. But the freeze-dried powder had a pleasant green color, low bulk density, high swelling and water holding capacity, and remarkably high phenolic content (718.00 mg GAE/100 g) and DPPH inhibition (64.14%).

Effects of Methanol Extract of Celery (Apium graveolens) Leaf on Ethanol-Induced Left Ventricular Changes in Wistar Rats

Background: Alcohol consumption is associated with several cardiovascular diseases such as heart failure, left ventricular hypertrophy, as well as with other disorders. This study was designed to determine the cardioprotective effect of celery (Apium graveolens) extract in attenuating perturbations arising from alcohol ingestion on left ventricular injury in adult Wistar rats.  Methods: Thirty male Wistar rats weighing 120g -160g were used for this study. The rats were divided into six (6) groups A, B, C, D, E &F five (5) rats each. The rats in group (A) served as control and received standard pellet, while groups B, C, D, E and F were administered orally with 8ml/kg b. w of ethanol and 100mg/kg, 150mg/kg, 200mg/kg of methanol extract of Apium graveolens (MEAG) were given to group C, D and E and 25mg of propranolol was given to group F orally. Group A and B were administered with 2ml/kg b. w distilled water.  Results: The relative heart weight, level of serum cardiac troponin I and left ventricular wall thickness of the ethanol-induced rats were significantly increase (F = 7.64; p = 0.002), (F = 22.66; p = 0.001) and (F = 85.29; p < 0.001) when compared with the control and across all experimental groups in this study. Group treated with MEAG also showed regular striations.Conclusion: This study indicates that MEAG has cardioprotective effects on these perturbations.

Effects of ultrasound-assisted plasma-activated water washing on the inhibition of natural microorganisms on fresh-cut celery and celery quality properties.

The effects of ultrasound (US)-assisted plasma-activated water (PAW) washing (UP) on the growth of indigenous bacteria, quality properties, and microbiome of fresh-cut Apium graveolens (celery) were investigated. Among the tested treatments (UP, NaClO solution, US alone, and PAW alone), UP was the most effective in decontaminating fresh-cut celery and inhibiting the growth of natural bacteria in the celery during storage at 4 and 10°C. No significant differences were observed in the firmness, color, or sensory properties between untreated and UP-treated fresh-cut celery during storage. The proportion of Proteobacteria and Actinobacteria on UP-treated fresh-cut celery decreased after storage at 4°C for 3days, confirming the influence of UP treatment on the celery microbiome. The UP treatment induced alterations in the microbial composition of celery. These results demonstrate the potential utility of UP treatment as a novel microbial decontamination process for the preparation of fresh-cut celery products.

Apium graveolens Herbs Ethanolic-Extract Improve Acetic Acid–Induced Colitis Condition in Rats

Apium graveolens Herbs Ethanolic-Extract Improve Acetic Acid–Induced Colitis Condition in Rats

Cardiovascular Effects of Herbal Products and Their Interaction with Antihypertensive Drugs-Comprehensive Review.

Hypertension is a highly prevalent population-level disease that represents an important risk factor for several cardiovascular complications and occupies a leading position in mortality statistics. Antihypertensive therapy includes a wide variety of drugs. Additionally, the potential antihypertensive and cardioprotective effects of several phytotherapy products have been evaluated, as these could also be a valuable therapeutic option for the prevention, improvement or treatment of hypertension and its complications. The present review includes an evaluation of the cardioprotective and antihypertensive effects of garlic, Aloe vera, green tea, Ginkgo biloba, berberine, ginseng, Nigella sativa, Apium graveolens, thyme, cinnamon and ginger, and their possible interactions with antihypertensive drugs. A literature search was undertaken via the PubMed, Google Scholar, Embase and Cochrane databases. Research articles, systematic reviews and meta-analyses published between 2010 and 2023, in the English, Hungarian, and Romanian languages were selected.

Effect of Celery (Apium graveolens L.) Extract Administration on Kidney Function of White Rats (Rattus norvegicus) Wistar Strains Exposed to Lead

Lead significantly affects health because of its sensitivity to the central nervous system and rapid absorption rate. The phytochemicals included in celery, such as flavonoids, may mitigate lead-induced kidney damage. This research aimed to determine how administering celery extract affected the expression of the Caspase-3 gene and the kidney TNF-α gene in Rattus Norvegicus Wistar white rats exposed to lead. The research method true experiment examined the effects of 80 mg/day celery extract on Caspase-3 gene expression and renal TNF-α gene expression in rats exposed to lead. Each of the three groups will include six rats; there will be eighteen male Wistar rats in the study. The study used SPSS 26.0 and the Saphiro Wills test to analyze data on TNF-α and Caspase 3 expression in rats exposed to lead acetate (Pb). The results showed a normal distribution for the negative control group (K-) and the positive control group (K+) and a normal distribution for the treatment group (P). However, the paired t-test showed significant differences in all treatment groups (p <0.05), indicating a normal distribution in the data. The study concludes the impact of celery extract on kidney function in lead-exposed Wistar rats, revealing various active compounds like flavonoids. Despite insignificant caspase 3 reduction, flavonoids showed potential in mitigating lead-induced kidney damage.

The role of chlorine promoters in mediating particle size effects in silver-catalyzed ethylene epoxidation

Measured chlorine coverages over Ag/α-Al2O3 catalysts with varying Ag weight loadings (1.3–35 wt%) and different Ag particle size distributions when parsed in terms of Cl coverages over particles of varying size constituted in the distribution reveal that Ag particles of size below ∼30 nm are covered in more than 1 monolayer (ML) of chlorine in presence of 3.5 ppm C2H5Cl. These assessments of Cl coverages were made possible by correlating the cumulative Cl coverage to the lognormal surface area distribution of 22 Ag/α-Al2O3 samples using a single exponential decay function. Fully chlorided small Ag particles exhibit low rates of epoxidation and only large Ag particles which exhibit sub-monolayer Cl coverages catalyze ethylene epoxidation with high ethylene oxide (EO) rates and selectivity. The Ag particle size dependence of Cl coverages plausibly explains why ≥ 100 nm Ag particles are typical in promoted Ag/α-Al2O3 ethylene epoxidation catalysts.

Revealing the structural evolution of CuAg composites during electrochemical carbon monoxide reduction

Comprehending the catalyst structural evolution during the electrocatalytic process is crucial for establishing robust structure/performance correlations for future catalysts design. Herein, we interrogate the structural evolution of a promising Cu-Ag oxide catalyst precursor during electrochemical carbon monoxide reduction. By using extensive in situ and ex situ characterization techniques, we reveal that the homogenous oxide precursors undergo a transformation to a bimetallic composite consisting of small Ag nanoparticles enveloped by thin layers of amorphous Cu. We believe that the amorphous Cu layer with undercoordinated nature is responsible for the enhanced catalytic performance of the current catalyst composite. By tuning the Cu/Ag ratio in the oxide precursor, we find that increasing the Ag concentration greatly promotes liquid products formation while suppressing the byproduct hydrogen. CO2/CO co-feeding electrolysis and isotopic labelling experiments suggest that high CO concentrations in the feed favor the formation of multi-carbon products. Overall, we anticipate the insights obtained for Cu-Ag bimetallic systems for CO electroreduction in this study may guide future catalyst design with improved performance.

Phenolic profile, and safety evaluation of the Moroccan aqueous polyherbal formulation containing Petroselinum crispum L., Coriandrum sativum L., Apium graveolens L.: Acute and sub-acute toxicity

Medicinal plants harbor numerous natural bioactive compounds with therapeutic potential, utilized in the synthesis of various drug formulations. While the diverse benefits of herbal formulations have been extensively researched, a toxicity assessment is crucial to establish a wide margin of safety for the therapeutic application of this polyherbal formulation. The recent study aimed to assess the phytochemical profile and potential toxic effects of an aqueous extract derived from a polyherbal formulation containing Petroselinum crispum L., Coriandrum sativum L., and Apium graveolens L. The investigation involved acute and sub-acute toxicity studies conducted in male and female Swiss albino mice, as well as adult rats. High-performance liquid chromatography analysis revealed a rich composition of phytochemical compounds in the extract. In acute toxicity assessments, oral administration of the extract up to 14 g/kg showed no signs of toxicity or fatalities in mice. However, intraperitoneal administration resulted in dose-dependent toxicity, with a calculated LD50 of 11.8 g/kg. Sub-acute toxicity studies in rats over 28 days showed no significant changes in organ weights, hematological, or biochemical parameters, except for a minor decrease in WBC count. Histopathological examination revealed no morphological disturbances in the liver and kidneys, indicating a wide margin of safety for therapeutic use.

First Report of Celery mosaic virus Infecting Celery (Apium graveolens) in Indonesia

Celery mosaic virus (CeMV), member of genus Potyvirus, is reported for the first time in Indonesia, from celery plants (Apium graveolens) in a vegetable field at Berastagi, North Sumatera Province. The plants possessed mosaic and vein clearing symptoms on the leaves as typical of CeMV infection. Virus incidence was confirmed by RT-PCR using degenerate potyvirus primer which amplified partial coat protein and 3’-UTR of the viral genome. Phylogenetic tree analysis placed Indonesian CeMV isolates in one separated clade within CeMV group and shared 96.5%–96.7% nucleotide identity with exemplar isolate of CeMV.

Therapeutic potential of multi-targeting phytochemicals derived from Apium graveolens ethanol extract in West Java, Indonesia against multidrug-resistant Pseudomonas aeruginosa

Abstract. Sufa HI, Kurniati I, Dermawan A, Abror YK, Indra AIN, Purkon DB. 2024. Therapeutic potential of multi-targeting phytochemicals derived from Apium graveolens ethanol extract in West Java, Indonesia against multidrug-resistant Pseudomonas aeruginosa. Biodiversitas 25: 2183-2190. This study aims to evaluate the antibacterial efficacy of Apium graveolens (AG) extract from West Java, Indonesia against MDR-P. aeruginosa species responsible for nosocomial infection. Several assessments of antibacterial activity in vitro were conducted, including the enumeration of bacterial colonies, the measurement of the inhibition zone by agar well diffusion, determination of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) values, and the observation of cell damage using Scanning Electron Microscopy (SEM). The results have demonstrated that AG extract from West Java, Indonesia, possesses significant potential as an antimicrobial agent against MDR-P. aeruginosa. The Apium graveolens extract, at concentrations of 50, 100, and 200 mg/mL, reduced MDR-P. aeruginosa colonies by 59.3%, 65.7%, and 82%, respectively. The inhibition zone sizes ranged from 7 to 12 mm for 50 mg/mL, 8 to 16 mm for 100 mg/mL, and 16 to 17 mm for 200 mg/mL. The MIC values of 100 mg/mL for PA1 and PA3, 200 mg/mL for PA2, with an MBC value of 200 mg/mL for each test. Scanning electron microscope observation indicated that treatment with 100 mg/mL of AG extract caused significant damage to bacterial cells. In conclusion, A. graveolens extracts show promise as potential antibacterial agents against nosocomial infections caused by MDR-P. aeruginosa. Further research, including in vivo studies of the antibacterial mechanism of action, is warranted.

Vegan gummy candies with low calorie based on celery (Apium graveolens) puree and boswellia gum (Boswellia thurifera).

Gummy candy is one of the main snacks for children, and conventional samples with high calorie illustrate no nutritional value; therefore, the aim of present research was to develop functional product on priority. Celery (Apium graveolens) puree (25%-50%), boswellia gum (10%-20%), lemon essential oil (0.25%-0.50%), and sugar (10%-20%) in two levels were considered for vegan gummy candy production. Based on central composite design, the 30 types of gummy candies were prepared; afterward, response surface methodology was applied to optimize results determined by texture (hardness, springiness, adhesiveness, gumminess, chewiness, and elasticity characteristics), physicochemical attributes (pH, sugar content, water activity, antioxidant function, and calorie restriction), and also sensory evaluation. In general, elevated concentration of celery puree and boswellia gum-enhanced hardness, chewiness, and also gumminess for treated products. On the other hand, higher sugar with lemon essential oil improved adhesion, springiness, and elasticity features. More boswellia gum, celery, lemon essential oil, and reduction in sugar elevated water activity and also declined pH for treated samples. The celery puree, boswellia gum, and lemon essential oil significantly enhanced antioxidant function of treated gummy candies. According to attained results, sugar had a remarkable influence on acceptability and in treated samples calorie decreased. Based on all investigated factors, optimal formulation was achieved including 25% celery puree, 20% boswellia gum, 0.450% lemon essential oil, and 13.55% sugar. Regarding the results, obtained gummy candy with high nutritional value and low calorie demonstrated the potential to produce extensively in food sector.

Dl-3-n-butylphthalide promotes microglial phagocytosis and inhibits microglial inflammation via regulating AGE-RAGE pathway in APP/PS1 mice

Alzheimer's disease (AD) stands as the most prevalent neurodegenerative condition worldwide, and its correlation with microglial function is notably significant. Dl-3-n-butylphthalide (NBP), derived from the seeds of Apium graveolens L. (Chinese celery), has demonstrated the capacity to diminish Aβ levels in the brain tissue of Alzheimer’s transgenic mice. Despite this, its connection to neuroinflammation and microglial phagocytosis, along with the specific molecular mechanism involved, remains undefined. In this study, NBP treatment exhibited a substantial improvement in learning deficits observed in AD transgenic mice (APP/PS1 transgenic mice). Furthermore, NBP treatment significantly mitigated the total cerebral Aβ plaque deposition. This effect was attributed to the heightened presence of activated microglia surrounding Aβ plaques and an increase in microglial phagocytosis of Aβ plaques. Transcriptome sequencing analysis unveiled the potential involvement of the AGE (advanced glycation end products) -RAGE (receptor for AGE) signaling pathway in NBP’s impact on APP/PS1 mice. Subsequent investigation disclosed a reduction in the secretion of AGEs, RAGE, and proinflammatory factors within the hippocampus and cortex of NBP-treated APP/PS1 mice. In summary, NBP alleviates cognitive impairment by augmenting the number of activated microglia around Aβ plaques and ameliorating AGE-RAGE-mediated neuroinflammation. These findings underscore the related mechanism of the crucial neuroprotective roles of microglial phagocytosis and anti-inflammation in NBP treatment for AD, offering a potential therapeutic target for the disease.

Chemical composition and biological activities of essential oils of seven Cultivated Apiaceae species

The Apiaceae family contains many species used as food, spice and medicinal purposes. Different parts of plants including seeds could be used to obtain essential (EO) oils from members of the Apiaceae family. In the present study, EOs were components obtained through hydrodistillation from the seeds of anise (Pimpinella anisum), carrot (Daucus carota), celery (Apium graveolens), dill (Anethum graveolens), coriander (Coriandrum sativum), fennel (Foeniculum vulgare), and cumin (Cuminum cyminum). EO constituents were determined with Gas Chromatography/Mass Spectrometry (GC–MS) and Gas Chromatography/Flame Ionization Detector (GC-FID) and their antioxidant capacities were determined with the cupric reducing antioxidant capacity (CUPRAC) and 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) methods. The antimicrobial activity of EOs were tested against four pathogenic bacteria. Phenylpropanoids in anise (94.87%) and fennel (92.52%), oxygenated monoterpenes in dill (67.59%) and coriander (98.96%), monoterpene hydrocarbons in celery (75.42%), mono- (45.42%) and sesquiterpene- (43.25%) hydrocarbons in carrots, monoterpene hydrocarbon (34.30%) and aromatic hydrocarbons (32.92%) in cumin were the major compounds in the EOs. Anethole in anise and fennel, carotol in carrot, limonene in celery, carvone in dill, linalool in coriander, and cumin aldehyde in cumin were predominant compounds in these EOs. The high hydrocarbon content in cumin EO gave high CUPRAC activity (89.07 µmol Trolox g−1), and the moderate monoterpene hydrocarbon and oxygenated monoterpene content in dill EO resulted in higher DPPH activity (9.86 µmol Trolox g−1). The in vitro antibacterial activity of EOs against Bacillus cereus, Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli was evaluated using the agar diffusion method and the minimum bactericidal concentration was determined. Coriander, cumin and dill EOs showed inhibitory effect against all tested strains except P. aeruginosa. While fennel and celery EOs were effective against E. coli and B. cereus strains, respectively, anise and carrot EOs did not show any antibacterial effect against the tested bacteria. Hierarchical Cluster Analysis (HCA) produced four groups based on EO constituents of seven species. The potential adoption of the cultivated Apiaceae species for EO extraction could be beneficial for the wild species that are endangered by over collection and consumption.

In vivo study of celery (Apium graveolens L) extract for regenerating pancreatic β-cell

Background: The celery plant (Apium graveolens L.) is a widely distributed herbal plant globally known for being a significant source of natural active products, including phenolic compounds and antioxidants demonstrating antidiabetic activity. Previous research findings have indicated the impact of celery leaf extract on reducing blood glucose levels. However, up to now, there is a dearth of research investigating the potential effect of celery leaf extract on the regeneration of pancreatic beta cells in mice with diabetes models.Objective: This study aims to determine the effect of the ethanol extract of celery leaves on the number of pancreatic cells in Streptozotocin (STZ)-induced mice.Method: This research method uses a randomized controlled group posttest only design. Experimental animals were divided into five groups, including a group of experimental animals in a healthy condition, the group with hyperglycemia, hyperglycemia group with celery extract at 200 mg/kg BW, hyperglycemia group with celery extract at 400 mg/kg, and hyperglycemia group with extract celery is 800 mg/kg BW. Termination was performed on day 60 after therapy and pancreas hematoxylin and eosin staining. They are counting the number of cells with a photo slide with 400x magnification in 5 fields of view—statistical analysis using One-Way ANOVA.Results: The study revealed that celery extract could increase the number of pancreatic cells compared to the control group (P<0.002). Notably, the highest increase in pancreatic cell count was observed in the group administered with 400 mg/BW of celery extract.Conclusion: Celery leaf extract effectively increased the number of beta cells in vivo.