Antioxidant Activity Of Methanolic Extracts Research Articles

Antimicrobial and Antioxidant Activities of Methanolic Extract and Fractions of <i>Epilobium roseum</i> (Schreb.) against Bacterial Strains

Present study aims to evaluate antimicrobial and antioxidant activities of the crude methanolic extract and different fractions of the Epilobium roseum (Schreb). The extract and fractions were used against pathogenic bacteria (Bacillus subtilis, Bacillus atrophaeus, Klebsiella pneumonia and Pseudomonas aeruginosa) and fungal strains (Aspergillus niger and Aspergillus flavus). The methanolic extract and their sub fraction n-hexane showed a prominent inhibition zone against all bacterial strains but inactive against fungal strains. The various extracts of Epilobium roseum (Schreb) from various parts were tested for their antioxidant activity by 1,1-Diphenyl-2-picryl-hydrazyl (DPPH) assay. The IC50 of the stem and root of methanolic extract and their sub fraction n-hexane showed best activity ranged between 22.73 ± 6.92, 21.49 ± 6.26 and 14.94 ± 3.54, 13.92 ± 1.04 μg/ml compared to another fraction. The results support that Epilobium roseum can be used as antimicrobial and antioxidant agents. The results support the present study that Epilobium roseum (Schreb) has a potential source of natural antibacterial, antifungal and antioxidant potentials.

Comparison of Phytochemical Composition, Free Radical Scavenging Activity, and Antimicrobial Activity of Selected Herbs Against Two Foodborne Pathogenic Bacteria

Spices and herbs, owing to their natural benefits to human health, are gaining momentum as food preservatives in recent years. Due to their antioxidant activity, their extracts with bioactive principles form the basis of pharmaceutical and food processing applications. Two of such crops, mountain mint (<i>Pycnanthemum virginianum</i>) and red turmeric (<i>Curcuma longa</i>), were compared for their phytochemical composition, the antioxidant activity of methanolic extracts, and their antimicrobial activity against foodborne pathogenic bacteria, Listeria monocytogenes, Salmonella enteritidis in this study. Our results showed that the mountain mint had higher total phenolic content and total tannin content: 614.41±3.96 and 529.74±4.39 mg gallic acid equivalents/gram dry weight extract. In comparison, red turmeric had higher total flavonoid content: 1250.51±8.10 mg catechin equivalents/gram dry weight extract. A lower IC₅₀ value (21.39±0.86 μg/mL) of red turmeric reflected its higher antioxidant activity. A lower concentration of methanolic extract of red turmeric rhizome was needed for both MIC (62.5 and 125 μg/mL) and MBC (125 and 250 μg/mL) assay against <i>L. monocytogenes</i> and <i>S. enteritidis</i>, respectively. In conclusion, these findings showed the red turmeric methanolic extract as a source of potential phytochemicals and antioxidants exhibited relative efficiency with antimicrobial activity against two common foodborne pathogens. The results have implications for use in processed food preservation and protection against food spoilage pathogens.

Antioxidant potential of crude extract, flavonoid-rich fractions, and a new compound from the seeds of Cordia dichotoma

The current study assessed the antioxidant activity of methanolic extract and different fractions of the seeds of Cordia dichotoma by 2,2-diphenyl-2-picrylhydrazyl hydrate method. Phytochemical screening of C. dichotoma seed extract was done using thin-layer chromatography technique and phytochemical methods. The percentage yield of secondary metabolites like alkaloids and saponins was also determined. The methanolic extract was subjected to isolation by Column Chromatography. Phytochemical screening revealed the presence of significant amounts of phenols and flavonoids in the extract. TLC analysis confirmed the presence of phytoconstituents with the application of derivatizing agents like aluminium chloride and anisaldehyde. Total phenolic and flavonoid contents obtained were 37.7 and 32.16% w/w, respectively. The crude seed extract of C. dichotoma showed inhibition at all concentrations in a dose-dependent manner. Maximum scavenging activity was exhibited by the methanolic extract with a low IC 50 value. A new compound named Cordioside was also isolated from the same extract. The phytochemical screening of the seed extract showed the presence of rich amounts of phenolic compounds and flavonoids, which may be acting as the key factors responsible for the antioxidant activity. The results revealed that methanolic extract and the aqueous fraction of C. dichotoma seed possess a significant antioxidant activity.

Experimental approaches targeting the biochemical and antioxidant potential of Synsepalum dulcificum dried fruits

This study aimed to investigate the biochemical content and antioxidant activity of methanol extracts prepared from Synsepalum dulcificum dried fruits. The results obtained by phytochemical screening of the extracts revealed the presence of some pharmacologically active chemical compounds such as: alkaloids, flavonoids, phenolic compounds and tannins. Quantitative analysis showed that the seeds had phenols in greater amounts (157.88 ± 2.09 mg GAE / g dry matter) than dried fruit pulp (54.09 ± 2.24 mg GAE / g dry matter). Contrariwise, flavonoid content was found to be low in seed (0.013 ± 0.02 mg EQ / g) and high in fruit pulp (0.13 ± 0.05 mg EQ / g). Correlated with the high amount of total phenol, in the seed extracts was found a good antioxidant activity – measured by both DPPH and ferric reducing antioxidant power (FRAP) assays. This antioxidant capacity may be also correlated with the amount of alkaloids, flavonoids, tannins and phenolic compounds, and as well as with their synergistic interactions. Synsepalum dulcificum fruits can be used as a healthy source of additives for the food industry or as natural antioxidants for the pharmaceutical industry.

GC-MS Analysis, Phytochemical Screening and Biological Activity of Bauhinia tomentosa (Linn.)

Present study aimed to evaluate the chemical composition and biological activity for methanolic extract of Bauhinia tomentosa (Linn.) leaves grown in Western Ghats region of South India. The preliminary phytochemical screening tests revealed the presence of steroids, alkaloids, terpenoids, flavonoids, glycosides and phenolic compounds in the leaf extract. A total of 19 compounds were identified through gas chromatography-mass spectroscopy (GC-MS) analysis of methanolic extract of B. tomentosa. The major compounds identified were phytol (23.96%), n-hexadecanoic acid (11.62%), squalene (8.85%) and the minor compounds are trans-bis(2-methylpropyl)-4,6-dioxane(0.13%), dihydro-cis-α-copaene-8-ol (0.14%), tetradecanoic acid (0.81%), respectively. Antibacterial activity of the extract showed the zone of inhibition 18 mm at 200 μg/mL against S. aureus, followed by 15 and 16 mm against S. anginosus, K. pneumoniae at 200 μg/mL, respectively. Antioxidant activity of methanolic extract of B. tomentosa leaves showed the maximum IC50 value with 75.07 % of scavenging activity at the concentration of 5 μg/mL.

Antioxidant Activities of Methanol Extracts of Thirteen Cameroonian Antibacterial Dietary Plants

This study falls within the search for alternative solutions to problems related to diseases associated with oxidative stress. It involved the evaluation of antioxidant activities extracts from thirteen antibacterial Cameroonian food plants, namely, P. nigrum, A. cruentus, L. sativa, S. edule, S. nigrum, V. amygdalina, A. hybridus, V. hymenolepis, L. capensis, M. esculenta, C. melo, T. occidentalis, and T. triangulare. The thirteen plant extracts with a broad spectrum of antibacterial activity all showed total reducing powers ranging between 2.41 and 27.81 AAE (mg ascorbic acid equivalents per gram of dried extract) and total phenol contents between 2.65 and 35.03 GAE (mg of gallic acid equivalents per gram of dried extract) of dry extract. Except for extracts of L. capensis, the other 12 extracts showed flavonoid contents ranging between 0.29 and 5.99 RE (rutin equivalents per gram of dried extract). All 13 plant extracts also showed free radical scavenging activity against DPPH· with IC50 ranging between 1.65 and 310.52 μg/ml, while 12 of these extracts exhibited inhibitory activity against NO· radical (IC50 ranging from 19.77 to 157.72 μg/ml). Statistically insignificant positive correlations (p&gt;0.5) were found between antibacterial activities of these plants and their antioxidant activities. The different results of this study provide scientific evidence for the use of these antibacterial food plants in the control of different conditions associated with oxidative stress.

Effect of heat treatment on polyphenolic compounds and antioxidant activity of lentils (Lens culinaris)

Abstract The current research involves the study of the effect of thermal treatment in boiling water (cooking) on the nutrient components and phytochemical contents of lentils. The changes of phenolic compounds and antioxidant activity of lentils before and after cooking; as well as the recovered water are investigated. Total polyphenol, flavonoid and tannin content were determined spectrophotometrically using standards methods and LC-MS/MS. Antioxidant activity of methanolic extracts was evaluated using DPPH (2,2-diphenyl-lpicrylhydrazyl) and ABTS (2,2’-azinobis-(3-ethyl benzo thiazoline-6-sulfonic acid)) assays. As compared to the raw lentils, cooking caused significant (p&lt;0.05) decreases in total phenolic content (TPC), total flavonoid content (TFC), DPPH and ABTS free radicals scavenging activity. The obtained results indicated that the polyphenol content of cooking water was significantly higher than that of cooked and raw lentils; however the most important antioxidant activity was recorded for raw lentils. Several phenolic compounds were identified in fresh, cooked lentils and cooking water. The major compound for the three samples is tannic acid with a very remarkable value in the cooking water; while the minor compounds are naringenin for cooked lentils, apigenin for raw lentils and cooking water. Neoformed compounds have been also detected in the cooking water such as: quercetin, hespritin, tr-caffeic acid and chlorogenous acid in the cooking water.

Phenolic compounds and antioxidant activity of methanolic extracts from leaves and flowers of chilcuague ( Heliopsis longipes , Asteraceae)

Background: Heliopsis longipes root is the widely used organ from the plant due to its culinary and medicinal importance. Nevertheless, during its collection the foliage is discarded. Therefore, it is required to perform studies about the phytochemical content with biological activity present in the different plant organs of the foliage, for a better exploitation of the plant.&#x0D; Hypothesis: The concentration of phenolic compounds with antioxidant activity varies according to the type of plant organ and the geographic location of the plant collection.&#x0D; Study species: Heliopsis longipes &#x0D; Site and years of study: The H. longipes plants were collected in three locations (Conca, San Juan Buenaventura and Beltran) from Sierra Gorda of Mexico, between 2017 and 2018.&#x0D; Methods: In this study, they were analyzed the methanolic extracts from H. longipes leaves and flowers, in order to determine the content of the phenolic compounds and scavenging of radicals activity (DPPH• and ABTS•+). Further characterization of the extracts was carried out by chromatography (UPLC).&#x0D; Results: The total content of polyphenols, flavonoids and condensed tannins varied in depending on the type of plant organ and the origin site of H. longipes , being the leaves of Beltran those that represent the best source of phenolic compounds with scavenging activity of the radicals DPPH• and ABTS•+. In the extracts, phenolic acids and flavonoids were identified.&#x0D; Conclusions: The geographical climatic conditions influence the phenolic content and antioxidant activity of the methanolic extracts from leaves and flowers of H. longipes .

Total Phenolic and Flavonoid Content, Antioxidant Activity of Talaromyces funiculosus Strains

Microscopic fungi synthesize a wide range of phenolic compounds that play an important role in cell wall formation, as well as perform signaling and protective functions. One of the important functions of phenolic compounds, primarily polyphenols, is antioxidant activity. The most active polyphenols are flavonoids, their synthesis in microscopic fungi has not been studied enough. The aim of the study was to determine the total phenols and flavonoids content and antioxidant activity of Talaromyces funiculosus strains isolated from different econiches. Methods. 20 strains of the microscopic fungus T. funiculosus – 10 endophytes and 10 saprotrophs were used in the experiments. Cultivation of filamentous fungi was performed under submerged conditions with shaking at 210–230 rpm in Erlenmeyer flasks containing 200 ml of Czapek liquid medium at a temperature 26±2°C for 6 days. The biomass of the strains was filtered, dried at 70°C and extracted with 95% methanol (1:50 w/v). The total phenols content was determined using Folin-Ciocalteu reagent by the method of Arora, Chandra (2010) with some modifications and expressed as mg of gallic acid equivalents (GAE) per g of dry weight mycelium. Flavonoids in each extract were determined spectrophotometrically with AlCl3 and expressed as mg of quercetin equivalents (QE) per g of dry weight mycelium. Antioxidant activity of methanolic extracts was measured as radical scavenging activity by the percentage inhibition of the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical. Results. The total phenolic content of methanolic extracts of T. funiculosus strains ranged from 0.12±0.007 to 1.97±0.150 mg GAE/g. The highest level of phenolic compounds was found for saprotroph UCM F-16790 (1.97±0.150) and endophyte UCM F-16795 (1.71±0.137 mg GAE/g). The flavonoid content in methanolic extracts of the studied strains varied in the range of 0.02±0.001–0.39±0.041 mg QE/g. All studied strains of T. funiculosus showed antioxidant activity against DPPH radical, which was 12.19±2.34–60.37±7.24% inhibition. Statistical analysis revealed a strong positive correlation between the total phenols and flavonoids content (Pearson’s correlation coefficient r=0.92); between antioxidant activity, phenols (r=0.82) and flavonoids (r=0.81) content in methanol extracts of T. funiculosus strains. The total content of phenols and flavonoids and antioxidant activity did not depend on the trophic group of the studied strains. The moderate correlation between flavonoids content and antioxidant activity of methanolic extracts of endophytic T. funiculosus strains (r=0.46) may be related to their ability to synthesize a wider range of polyphenolic compounds with antioxidant activity. Conclusions. The studied T. funiculosus strains have the ability to synthesize phenolic compounds and flavonoids in different concentrations. Correlation analysis revealed strong correlations between total phenolic and flavonoid contents and antioxidant activity. The reason for phenomenon of moderate correlation (r=0.46) between flavonoid content and antioxidant activity in endophytic strains may be the synthesis of other compounds by individual strains. The determination of individual components synthesized by the most active strains is of scientific interest for further study. T. funiculosus UСM F-16795 and UСM F-16790 strains had a quite high level of phenols and flavonoids. Obtained data may be promising for the search for phenolic compounds with antioxidant properties to increase plant resistance to negative impacts of environmental stress factors.

Antimicrobial and antioxidant activity of methanol extract of Echinophora spinosa L. from Jijel, Algeria

Antimicrobial and antioxidant activity of methanol extract of Echinophora spinosa L. from Jijel, Algeria

A Study on the Antioxidant Activity of Methanolic Extracts from Leaf, Stem and Flower Parts of Astragalus ovatus DC.

The present study investigated the antioxidant activity of flower, stem and leaf extracts of Astragalus ovatus DC. plant. Total phenolic and flavonoid contents were determined in methanolic extracts of Astragalus ovatus DC flower, stem and leaves. Leaf of Astragalus ovatus DC. exhibited the highest activity of those parts tested. Total phenolic content and total flavonoid content for this part of plant were determined as 6.88 mg GAE/g and 2.80 mg QUE/g, respectively. FRAP value was found as 30.50 μmol FeSO4.7H2O/g. This is the first study to report antioxidant activity of flower, stem and leaf extracts of Astragalus ovatus DC.

Antimicrobial, antioxidant and phytochemical investigation of Thuja occidentalis (Arbor vitae) leave extract

Various medicinal plants are common in use in India for the treatment of different diseases. Nowadays, drug resistance has emerged as a major problem for various infections, in such case plants can be used as alternative for the production of new medicine. Thuja occidentalis (T. occidentalis, Cupressacae) is a well-known medicinal plant. The aim of the present study was to evaluate qualitative and quantitative phytochemical analysis and in vitro antioxidant and antimicrobial activities of leaf of T. occidentalis collected from Bhopal region of Madhya Pradesh. Qualitative analysis of various phytochemical constituents and quantitative analysis of total phenolics and flavonoids were determined by the well-known test protocol available in the literature. Quantitative analysis of phenolic and flavonoids was carried out by Folins Ciocalteau reagent method and aluminium chloride method respectively. The in vitro antioxidant activity of methanolic extract of the leaves was assessed against DPPH and reducing power assay method using standard protocols and in vitro antimicrobial activity was done by well diffusion assay method. Phytochemical analysis revealed the presence of alkaloids, glycosides, phenols, flavonoids, tannins. The total phenolics content of leaves of methanolic extract was (0.125 mg/gm), followed by flavonoids (0.475mg/gm). The activities of all leaves extract against DPPH and reducing power assay method were concentration dependent. The antimicrobial acuity of methanolic extract of leave against all microorganisms was concentration dependent manner but less than standard drug (Ofloxacin, Amphotericin B). The present study concluded that the crude extract of T. occidentalis is a rich source of secondary phytoconstituents which impart significant antioxidant and antimicrobial potential. The findings of the present study will be helpful to phytochemists, pharmacologists and pharmaceutical industries.

Activités antioxydantes in vitro des extraits aqueux et méthanoliques des calices de Hibiscus sabdariffa provenant deux localités du Cameroun

Background: Fighting against malnutrition and diseases such as oxidative stress diseases via a food-based approach could be achieved through identification, valorization, and promotion of local foods rich in macro- and micronutrients and phytochemical components. Aim: This study aimed to investigate the effect of agro-ecological conditions on the antioxidant capacity of the Hibiscus sabdariffa aqueous and methanol calyces extracts. Material and methods: The total phenolic content, the free radical DPPH• (1,1-diphenyl-2-picrylhydrazyl), and ferric reducing antioxidant power (FRAP) activities were evaluated in aqueous and methanol Hibiscus sabdariffa calyces extract samples from two localities (Dschang [western highland zone] and Ngaoundéré [high Guinean savanna zone]) in Cameroon. Results: The results obtained showed that these extracts contain an important amount of total phenolic compounds with no significant difference (P&gt;0.05) between aqueous and methanol extracts and also between the origins of calyces. The extracts showed their ability to reduce Fe3+ to Fe2+ as well as their ability to reduce the free radical, DPPH•. Concerning the FRAP results, at the concentration of 12.5µg/mL, the absorbance of AEN (Aqueous Extract from Ngaoundéré locality) recorded the value (0.33±0.05) meanwhile at a far higher concentration of 200 µg/mL, the absorbance of MEN (Methanol Extract from Ngaoundéré locality) was the highest (1.39±0.06). The CI50 of methanol extracts was significantly low (P&lt;0.05) compared to that of aqueous extracts. The value ranges were 11.31±0.15, 14.69±0.84, 18.07±0.63, 20.50±0.54, and 21.50±0.54 µg/mL for ascorbic acid, MEN (Methanol Extract from Ngaoundéré locality), MED (Methanol Extract from Dschang locality), AEN (Aqueous Extract from Ngaoundéré locality), and AED (Aqueous Extract from Dschang locality) respectively. Conclusion: These findings show that the antioxidant activity of methanol extract is higher than that of aqueous extract. Hibiscus calyces from the two localities could be used as a natural antioxidant in the treatment of oxidative stress diseases. Keywords: Roselle calyces, aqueous and methanol extracts, antioxidant capacity.

In vitro, antioxidant activities of aqueous and methanol roselle (Hibiscus sabdariffa) calyces extracts from two localities in Cameroon

Background: Fighting against malnutrition and diseases such as oxidative stress diseases via a food-based approach could be achieved through identification, valorization, and promotion of local foods rich in macro- and micronutrients and phytochemical components. Aim: This study aimed to investigate the effect of agro-ecological conditions on the antioxidant capacity of the Hibiscus sabdariffa aqueous and methanol calyces extracts. Material and methods: The total phenolic content, the free radical DPPH• (1,1-diphenyl-2-picrylhydrazyl), and ferric reducing antioxidant power (FRAP) activities were evaluated in aqueous and methanol Hibiscus sabdariffa calyces extract samples from two localities (Dschang [western highland zone] and Ngaoundéré [high Guinean savanna zone]) in Cameroon. Results: The results obtained showed that these extracts contain an important amount of total phenolic compounds with no significant difference (P&gt;0.05) between aqueous and methanol extracts and also between the origins of calyces. The extracts showed their ability to reduce Fe3+ to Fe2+ as well as their ability to reduce the free radical, DPPH•. Concerning the FRAP results, at the concentration of 12.5µg/mL, the absorbance of AEN (Aqueous Extract from Ngaoundéré locality) recorded the value (0.33±0.05) meanwhile at a far higher concentration of 200 µg/mL, the absorbance of MEN (Methanol Extract from Ngaoundéré locality) was the highest (1.39±0.06). The CI50 of methanol extracts was significantly low (P&lt;0.05) compared to that of aqueous extracts. The value ranges were 11.31±0.15, 14.69±0.84, 18.07±0.63, 20.50±0.54, and 21.50±0.54 µg/mL for ascorbic acid, MEN (Methanol Extract from Ngaoundéré locality), MED (Methanol Extract from Dschang locality), AEN (Aqueous Extract from Ngaoundéré locality), and AED (Aqueous Extract from Dschang locality) respectively. Conclusion: These findings show that the antioxidant activity of methanol extract is higher than that of aqueous extract. Hibiscus calyces from the two localities could be used as a natural antioxidant in the treatment of oxidative stress diseases. Keywords: Roselle calyces, aqueous and methanol extracts, antioxidant capacity.

Hypoglycemic, antidyslipidemic, and antioxidant activities of methanol extract of Struchium sparganophora leaves in alloxan-induced oxidative stress-mediated diabetes in rats

BackgroundDiabetes mellitus is clinically underlined by hyperglycemia and dyslipidemia. In view of this, the current study assessed the glycemic and lipidemic control potentials of methanol extract of Sruchium sparganophora leaves (SPA) in the alloxan-induced diabetic model using male Wistar rats. Experimental diabetes was induced through a single intraperitoneal injection of 120 mg/kg freshly prepared alloxan. Thirty-six rats were randomly assigned into six groups of normoglycemic control, untreated diabetic group, and diabetic treated with (i) metformin (12 mg), (ii) metformin 12 mg + SPA 300 mg/kg, (iii) SPA 300 mg/kg, and (iv) SPA 600 mg/kg per os twice at 9.00 and 18.00 h daily for 10 days. Fasting blood glucose (FBG) level and markers of dyslipidemia, and oxidative stress markers were determined.ResultsSPA at selected doses decreased fasting blood glucose which was significantly (p < 0.05) raised by alloxan. Increase in plasma total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and triglyceride (TG) concentrations and decrease in HDL-cholesterol (HDL-C) concentration (p < 0.05) caused by alloxan were significantly moderated by SPA at selected doses. Glutathione-s-transferase (GST), superoxide dismutase (SOD), and catalase (CAT) activities reduced by alloxan (p < 0.05) in both the liver and pancreas were reversed by SPA 300 and 600, and its combination with metformin. Decreased reduced glutathione (GSH) concentration in alloxan diabetic rats was also reversed by the extract, while the level of malondialdehyde (MDA) exacerbated by alloxan (p < 0.05) in the tissues was decreased by the extracts.ConclusionStruchium sparganophora possesses considerable antihyperglycemic, antidyslipidemic, and antioxidant potentials without compromising organ functionality.

Phytochemical and antioxidant activities of methanolic extract of Lawsonia inermis L. Bark

Abstract Many diseases are associated with oxidative stress are caused by free radicals. Current research has been directed towards finding naturally occurring antioxidants of plant origin. The aim of the present study was to evaluate the in vitro antioxidant activities of methanolic extract of Lawsonia inermis L. bark (MELIB). The present study was carried out for determination of qualitative, quantitative phytochemical and in vitro antioxidant activity for scavenging of free radical DPPH, superoxide radical scavenging and lipid peroxidation. The bark extract is a rich source of secondary metabolites like flavonoids at a rutin equivalent 73.43±0.26%, polyphenol at a gallic acid equivalent 84.70±0.43%, tannins at a tannic acid equivalent 88.75±0.14% and terpenoids at a linalool equivalent 68.13±0.31. MELIB showed free radicals scavenging capacity by way of the DPPH method (6.93±0.51 to 62.63±0.10% inhibition), the superoxide radical scavenging method (1.82±0.41 to 57.11±0.18% inhibition) and the ferric chloride induced lipid peroxidation method (9.87±0.33 to 80.32±0.82% inhibition). The results obtained in the present study indicate that MELIB can be a potential source of natural antioxidants due to the presence of flavonoids, polyphenols, tannins and terpenoids.

Antioxidant property and phenol content of some medicinal plants from Sonitpur district of Assam

The present experiment was conducted in the Department of Molecular Biology and Biotechnology, Tezpur University during 2007-08. In this experiment, nine indigenous medicinal plants are analysed for their total phenolic contents and antioxidant activity viz. Amaranthus spinosus, Centella asiatica, Chenopodium album, Houttuyniacordata, Moringa oleifera, Paederia foetida and Polygonum chinense. The antioxidant activity of methanol extracts of the plants were determined by Spectrophotometric method using the stable free radical DPPH (2, 2-diphenyl-1-picrylhydrazyl). The results revealed that Moringa oleifera leaves recorded highest total phenolic content and exhibited highest percent inhibition of free radical followed by Houttuynia cordata. While, the lowest total phenolic content and lowest percent inhibition of free radical was recorded in Chenopodium album.

Biochemical, Mineral Analysis and Anti-Oxidant Activity of Methanolic Extracts of Ulva Flexuosa and Hypnea Valentiae

Biochemical, Mineral Analysis and Anti-Oxidant Activity of Methanolic Extracts of Ulva Flexuosa and Hypnea Valentiae

Antimicrobial and antioxidant activity of methanol extract of Echinophora spinosa L. from Jijel, Algeria

In this study, the antioxidant and antimicrobial activities of Echinophora spinosa were investigated. Antimicrobial activity of methanol extracts obtained from Echinophora spinosa was examined using the disc diffusion method. Antioxidant activity of the methanol extracts was examined using the stable 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging test. Methanol extract of ripe fruits of E. spinosa showed highest total phenolic content (69.17 ± 1.2 µg GAE/mg extract) and the major flavonoid contents (12.122 ± 0.44 µg QE/mg extract) was found in leaves of the plant. In disk diffusion antimicrobial assay, E. spinosa manifested broad spectrum of activity. The largest capacity to neutralize DPPH radicals was found for ripe fruits methanol extract of E. spinosa plant. The results shows that the various parts of E. spinosa extracts promising antioxidant and antimicrobial activities have potential bioactivities due to high content of phenolic and flavonoid compounds.

Phytochemical screening and antioxidant activity of methanolic extracts of 53 antimalarial plants from Bagira in Eastern DR Congo

Phytochemical screening and antioxidant activity of methanolic extracts of 53 antimalarial plants from Bagira in Eastern DR Congo