Antihypercholesterolemic Effects Research Articles

Antihypercholesterolemic effect of Cleome arabica L. on high cholesterol diet induced damage in rats.

Dietary cholesterol is known to be one of the main risk factors that accelerate oxidation process leading to hypercholesterolemia and attendant cardiovascular diseases. The purpose of this study, carried out on adult male Wistar rats, was to evaluate the inhibitory effects of supplementation with aqueous of Cleome arabica leaf extract on hypercholesterolemia. After 3 months of treatment, animals were sacrificed by decapitation. Blood serum was obtained by centrifugation. Under our experimental conditions, administration of Cleome arabica leaf extract decreased the total cholesterol (TC), LDL-cholesterol (LDL-chol) and triglycerides (TG) levels by 27 %, 52 %, 37 %, respectively, and reduced SGOT SGPT, LDH and PAL levels in blood serum compared to untreated hypercholesterolemic rats. TBARS concentrations decreased by 21 % in liver, 22 % in heart and 30 % in kidney in a group of rats treated with cholesterol and Cleome arabica (Chol C.ar) compared to a Chol-treated group. The same treatment with Cleome arabica leaf extract increased superoxide dismutase and enhanced glutathione peroxidase activity. Catalase activity was found to increase in liver, heart and kidney by 17 %, 16 % and 23 %, respectively, in the C.ar Chol-treated group. The protective effect of Cleome arabica on hypercholesterolemia inducing oxidative stress in several organs was mainly attributed to antioxidant properties. The latter were due to the presence of phenolic acids and flavonoids shown by the obtained HPLC profiles.

Antihypercholesterolemic Effects of Mushroom, Chrysin, Curcumin and Omega-3 in Experimental Hypercholesterolemic Rats

Hypercholesterolemia and hypertriglyceridemia are major risk factors that accelerate the incidence of atherosclerosis and coronary artery diseases. Therefore, the present study was conducted to evaluate the hypolipidemic effect of widely known traditional medicinal herbs and omega-3 FA in experimental hypercholesterolemia induced by Triton WR-1339. Experimental hypercholesterolemic rats were administered mushroom, chrysin, curcumin and omega-3 for 2 weeks. Hypercholesterolemic rats showed an increase in serum levels of lipid profiles and hepatic enzymes. Hypercholesterolemic rats showed an increase in malondialdehyde (MDA) levels and a decrease in both serum levels and mRNA expression of catalase, superoxide dismutase (SOD) and glutathione reducatse. Moreover, hypercholesterolemic rats showed hepatic down regulation in the expression of genes related to fatty acids oxidation such as acyl CoA oxidase (ACO) and synthetase (ACS), together with carnityl palmityl transferase-1 (CPT-1) and peroxisome proliferator activator receptor-α (PPAR-α). Administration of mushroom, chrysin, curcumin and omega-3 to hypercholesterolemic rats for 2 weeks up-regulated significantly the down regulated genes. In contrast, expression of genes related to fatty acids biosynthesis and cholesterol metabolism were increased in hypercholesterolemic rats compared to control group. Herbal medications and omega-3 administration down regulated genes of fatty acids biosynthesis and cholesterol metabolism to normal expression. At cellular levels, hyperlipidemia induced fatty droplets accumulation, necrosis and presence of apoptotic hepatocytes together with leukocytic infiltration in necrotic area that are ameliorated and normalized after administration of herbs and omega-3. In conclusion, the current findings indicated that flavonoids (mushroom, chrysin, curcumin) and omega-3 possess antihypercholesterolemic effects at biochemical, molecular and histopathological levels and are useful in treatment of hypercholesterolemia with lower side effects compared with synthetic hypolipidemic drugs.

Extraction of a soluble polysaccharide from Auricularia polytricha and evaluation of its anti-hypercholesterolemic effect in rats

Mushroom extracts are a new source of supplements for health and pharmaceutical due to their bioactivities. This study was to optimize the extraction parameters of a soluble polysaccharide from Auricularia polytricha (SPAP) by response surface methodology. The practical optimum parameters were an extraction time of 4h, an extraction temperature of 95°C and a ratio of water to fruiting bodies of 28mL/g, and the highest extraction rate was 19.77%. In vivo, male Sprague-Dawley (SD) rats were diet-induced hypercholesterolemic models and oral administration of SPAP to evaluate anti-hypercholesterolemic effects. The results showed that SPAP decreased the serum concentrations of blood lipid, made them close to the normal level. The total cholesterol in the SPAP consumption groups was significantly decreased 34.6±7.6% and 33.3±7.9% with dose of 4.5 and 9.0mg/kg BW in the 29th day. This study suggested that SPAP was a suitable natural agent and may be applied in therapy.

Shea Butter: An Opposite Replacement for Trans Fat in Margarine

Shea butter is the edible fat extracted from the nut of African Shea tree (Vitellaria paradoxa). Consequence of having half of its fatty acids saturated, Shea butter melts at a very high temperature and will be a suitable raw material for margarine production. Margarine is a butter mimicry that is produced from vegetable oils and water. The production of margarine requires a solid fat. Hence hydrogenation is employed to “harden” the vegetable oil. However, hydrogenation generates trans unsaturated fatty acids which are more detrimental to cardiovascular health than the highly denunciated saturated fatty acids. Since Shea butter is a stable solid at room temperature and has its saturated fatty acid fraction predominated by stearic acid, the use of Shea butter as a raw material for margarine will not only eliminate trans unsaturated fatty acids from the product but also make use of the least deleterious saturated fatty acid because stearic acid has been reported as the healthiest saturated fatty acid as regards cardiovascular health. Also, the unsaponifiables of Shea butter have been credited for their anti-hypercholesterolemic effects in experimental animals. This increases the healthfulness of dietary Shea butter, and of course, the margarine end product.

Anti-hypercholesterolemic effect of kenaf (Hibiscus cannabinus L.) seed on high-fat diet Sprague dawley rats.

To determine the antihypercholesterolemic effects of kenaf seed samples and compare with the commercial hypocholesterolemic drug on serum lipids profiles and malondialdehyde (MDA) level in the rat. Kenaf seed oil (KSO), microencapsulated kenaf seed oil (MKSO), kenaf seed extract (KSE) and defatted kenaf seed meal (DKSM) were prepared and phytochemicals screening on these samples were done prior in vivo study. Phenolic compounds in KSE were quantified using high performance liquid chromatography. There were 40 (divided in eight diet groups of 5) male Sprague dawley rats adapted to normal standard diet or hypercholesterolemic diet (HD) with or without the treatment of these kenaf samples for 32 days. All the kenaf samples exhibited to contain most of the major phytochemicals. KSE possessed gallic acid, tannic acid, catechin, benzaldehyde, benzoic acid, syringic acid, sinapic acid, ferulic acid, naringin acid, and protocatechuic acid. The significant higher (P<0.05) serum total cholesterol, low density lipoprotein cholesterol and MDA levels in HD group without treatment than the normal control group suggested the hypercholesterolemia was induced by the incorporation of cholesterol into diet. KSE exhibited higher cholesterol-lowering properties due to the significant lower (P<0.05) in serum triglycerides, total cholesterol and MDA levels. KSE showed the highest efficiency of cholesterol-lowering activity, followed by KSO, MKSO and DKSM. DKSM, MKSO, KSO and KSE appeared to have comparable anti-hypercholesterolemic effect with the commercial hypocholesterolemic drug. Hence, kenaf seed could be used as an alternative natural source to replace the synthetic hypocholesterolemic drugs.

HMG-CoA reductase inhibitory activity and phytocomponent investigation of Basella alba leaf extract as a treatment for hypercholesterolemia.

The enzyme 3-hydroxy-3-methyl-glutaryl-coenzyme A (HMG-CoA) reductase is the key enzyme of the mevalonate pathway that produces cholesterol. Inhibition of HMG-CoA reductase reduces cholesterol biosynthesis in the liver. Synthetic drugs, statins, are commonly used for the treatment of hypercholesterolemia. Due to the side effects of statins, natural HMG-CoA reductase inhibitors of plant origin are needed. In this study, 25 medicinal plant methanol extracts were screened for anti-HMG-CoA reductase activity. Basella alba leaf extract showed the highest inhibitory effect at about 74%. Thus, B. alba was examined in order to investigate its phytochemical components. Gas chromatography with tandem mass spectrometry and reversed phase high-performance liquid chromatography analysis revealed the presence of phenol 2,6-bis(1,1-dimethylethyl), 1-heptatriacotanol, oleic acid, eicosyl ester, naringin, apigenin, luteolin, ascorbic acid, and α-tocopherol, which have been reported to possess antihypercholesterolemic effects. Further investigation of in vivo models should be performed in order to confirm its potential as an alternative treatment for hypercholesterolemia and related cardiovascular diseases.

Anti-Hypercholesterolemic Effect of Saccharomyces boulardii in the Hamster

Background/Aims: Hypercholesterolemia is a major risk factor for coronary artery disease and probiotics have been suggested as tools to manage elevated cholesterol levels. Methods: The present study investigated the ability of the biotherapeutic agent Saccharomyces boulardii (Sb-Biocodex) to reduce the hypercholesterolemia induced by a 0.1% cholesterol-enriched diet in the hamster. Results: In a first experiment, chronic oral treatment with S. boulardii at 12  10¹⁰ CFU/kg (3 g/kg) twice a day was started from the beginning of the cholesterol diet and continued for 14 days (‘preventive protocol'). In the second experiment, S. boulardii was given 14 days after the beginning of the cholesterol diet when hypercholesterolemia had developed and continued for an additional 14 days (‘curative protocol'). In the preventive protocol, administration of the yeast significantly reduced hypercholesterolemia (14%) induced by the cholesterol-enriched diet compared to the group receiving only the cholesterol diet. In the curative protocol, S. boulardii significantly reduced hypercholesterolemia (12%) induced by the cholesterol-enriched diet, too. Moreover, the yeast significantly decreased the serum triglyceride increase by 39%. Conclusion:S. boulardii possesses anti-hypercholesterolemic properties in the hamster worthy of further evaluation in clinical studies. i 2014 S. Karger AG, Basel

Anti-hypercholesterolemic effect of Pistacia lentiscus fatty oil in egg yolk-fed rabbits: a comparative study with simvastatin

AimThe current study was undertaken to assess anti-hyperlipidemic activity of Pistacia lentiscus fatty oil (PLFO) in rabbits following a hyperlipidemic diet. MethodTwenty healthy female (WNZ) rabbits were divided into four groups of five animals each: (a) normal control (NC group) receiving standard diet, (b) hyperlipidemic control (EY) group receiving standard diet and gavaged daily with egg yolk (10 mL), (c) hyperlipidemic + PLFO (EY + PLFO) group receiving as the EY group and treated daily with PLFO (2 mL/kg BW, (d) hyperlipidemic + simvastatin (EY + SVS) group receiving as the EY group and treated once daily with 2.5 mg/kg BW of simvastatin. At the end of the six-week experimental period, the lipidemic profiles of the different groups were investigated. ResultsIn the EY group, the egg yolk resulted in a significant increase of total cholesterol (TC), triglycerides (TG), HDL-C, LDL-C, and the LDL-C/HDL-C ratio. Both the EY + PLFO and EY + SVS groups, when compared to the EY group, showed a significant decrease of TC, TG, LDL-C, and the LDL-C/HDL-C ratio. However, with respect to HDL-C the differences were not significant. The TGs were significantly lower (P < 0.001) in the simvastatin-treated group when compared to rabbits treated in the PLFO group. ConclusionThe study concludes that P. lentiscus fatty oil (PLFO) possesses anti-hyperlipidemic properties at least in reducing total cholesterol, LDL-cholesterol and triglycerides.

The antihypercholesterolemic effect of jatrorrhizine isolated from Rhizoma Coptidis

Current work was conducted to evaluate the safety and antihypercholesterolemic activity of jatrorrhizine extracted from Rhizoma Coptidis (RC) and its potential mechanism on regulating cholesterol metabolism. It was found that the LD50 of jatrorrhizine in mice was more than 5500mg/kg and there were no influences on clinical signs, organ weight changes, urinalysis and hematological parameters, gross necropsy and histological alterations in jatrorrhizine-treated rats during the 3-month period, compared to the control group. Jatrorrhizine showed a strong lipid-lowering effect in a dose-dependent manner. Oral administration of 70.05mg/kg of jatrorrhizine on Mesocricetus auratus (Syrian golden hamsters) exhibited significant decrease in TC, TG, and LDL-c levels by 20%, 43%, and 19%, respectively, and increase in HDL-c and total bile acids (TBA) content in feces (p<0.01), compared to high-fat and high-cholesterol (HFHC) group. Besides, jatrorrhizine dose-dependently slowed the rate of weight gain. The results of qRT-PCR, western blotting and ELISA revealed that jatrorrhizine significantly up-regulated the mRNA and protein expression of LDLR and CYP7A1, but exhibited no significant effect on mRNA and protein expression of HMGR and ASBT in hamsters.In conclusion, jatrorrhizine was a safe and potential antihypercholesterolemic agent from RC which could improve the utilization and excretion of cholesterol by up-regulating the mRNA and protein expression of LDLR and CYP7A1.

Role of Boswellia Ovalifoliolata Bal. Henry extract on high fat diet induced hypercholesterolemia

Objective: To evaluate the Antihypercholesterolemic effect of Boswellia ovalifoliolata Bal. Henry extract by performing invivo studies and to checkout its effects by evaluating parameters like food consumption, weight gain, fecal fat excretion, serum and liver lipid &biochemical profiles. Even the study includes confirmation of activity by the histopathological studies. Methods: Animals were fed with cholesterol rich high fat diet. Food intake, Bodyweight and fecal fat excretion were measured. Serum and liver samples were analyzed for the estimation of lipid profiles and other biochemical parameters by using different kits. Histopathological study on liver, aorta, heart and adipose tissue was done to ensure the activity. Results: The animal group administered with methanolic extract of the plant has shown decreased levels of TC, LDL, VLDL, TG, HDL+VLDL, VLDL+LDL, LDL/TC, AI, SGOT, SGPT and elevated levels of HDL, HDL/TC in a dose dependent manner significantly (p<0.01 & p<0.05). The evaluation of liver tissue of animal groups treated with herbal extract and standard had shown increased levels of SOD, GSH and Catalase, whereas levels of SGOT, SGPT, Total glucose, HMG-CoA, lipase, amylase and percentage of monaldehyde were decreased when compared with high fat diet fed rats. Body weight and Food intake in treated groups were significantly lower than that in model control. Conclusion: It can be confered from the present studies that the Boswellia ovalifoliolata Bal. Henry extract have strong activity against hypercholesterolemia and obesity suggesting a potential benefit as antihypercholesterolemic agent.

The Anti-Hypercholesterolemic Effect of Low p53 Expression Protects Vascular Endothelial Function in Mice

AimsTo demonstrate that p53 modulates endothelial function and the stress response to a high-fat western diet (WD).Methods and ResultsThree-month old p53+/+ wild type (WT) and p53+/− male mice were fed a regular or WD for 3 months. Plasma levels of total cholesterol (TC) and LDL-cholesterol were significantly elevated (p<0.05) in WD-fed WT (from 2.1±0.2 mmol/L to 3.1±0.2, and from 0.64±0.09 mmol/L to 1.25±0.11, respectively) but not in p53+/− mice. The lack of cholesterol accumulation in WD-fed p53+/− mice was ass–ociated with high bile acid plasma concentrations (p53+/− = 4.7±0.9 vs. WT = 3.3±0.2 μmol/L, p<0.05) concomitant with an increased hepatic 7-alpha-hydroxylase mRNA expression. While the WD did not affect aortic endothelial relaxant function in p53+/− mice (WD = 83±5 and RD = 82±4% relaxation), it increased the maximal response to acetylcholine in WT mice (WD = 87±2 vs. RD = 62±5% relaxation, p<0.05) to levels of p53+/−. In WT mice, the rise in TC associated with higher (p<0.05) plasma levels of pro-inflammatory keratinocyte-derived chemokine, and an over-activation (p<0.05) of the relaxant non-nitric oxide/non-prostacyclin endothelial pathway. It is likely that in WT mice, activations of these pathways are adaptive and contributed to maintain endothelial function, while the WD neither promoted inflammation nor affected endothelial function in p53+/− mice.ConclusionsOur data demonstrate that low endogenous p53 expression prevents the rise in circulating levels of cholesterol when fed a WD. Consequently, the endothelial stress of hypercholesterolemia is absent in young p53+/− mice as evidenced by the absence of endothelial adaptive pathway over-activation to minimize stress-related damage.

Impact of repeated administration of Cannabis sativa on some biochemical parameters in albino rats

Cannabis sativa is an illicit drug with proven anti-inflammatory, analgesic, antipyretic and anti diarrhea activities. This present study is aimed at ascertaining the impact of Cannabis sativa on some biochemical indices of albino rats. Twenty (20) male wistar albino rats ranging from180-200g were acclimated to laboratory conditions for 7days, following which they were randomly assigned into 4 groups i, ii, iii and iv of five animals each based on average body weight. Groups (ii-iv) were administered 0.5 ml aqueous extract of Cannabis sativa via oral route corresponding to 1, 2 and 3mg/Kg/body weight, while group i (control) received 0.5ml of distilled water orally. Rats in all groups were sacrificed 24hours after the experimental periods of 30daysoforaladministration of the extract. The extract significantly (p<0.05) increased serum Albumin, Globuli n, Total protein, Conjugated bilirubin while Total bilirubin, unconjugated bilirubin concentration showed a significant (p<0.05) increase only in group ii and iv when compared with the control. Likewise group ii and iii showed a significant (p<0.05) reduction in total cholesterol .The serum AST and ALT showed a significant (p<0.05) increase when compared with the control. The alterations by the results are manifestation of mild hepatorenal toxicity and anti-hypercholesterolemic effect.

Role of an Ethanolic Extract of Crotalaria juncea L. on High-Fat Diet-Induced Hypercholesterolemia.

ObjectiveTo evaluate the antihypercholesterolemic effects of 50 mg/kg BW and 100 mg/kg BW per day of an ethanolic extract of Crotalaria juncea Linn (whole plant) by performing in vivo studies.MethodsThe effects of oral administration of 50 mg/kg BW and 100 mg/kg BW per day of an ethanolic extract of Crotalaria juncea Linn (whole plant) in rats fed with a high-fat diet were investigated by evaluating parameters like food consumption, weight gain, fecal fat excretion, serum and liver lipids, and biochemical profiles as well as by histopathological studies. The results were compared to animals fed with the standard diet and animals fed with a high-fat diet and atorvastatin (10 mg/kg BW).ResultsThe animal group administered with the ethanolic extract for 35 days showed decreased levels of TC, LDL, VLDL, TG, HDL+VLDL, VLDL+LDL, LDL/TC, AI, SGOT, SGPT, and elevated levels of HDL, HDL/TC, significantly (p<0.01 & p<0.05) in a dose-dependent manner. The evaluation of liver tissues of the animal groups treated with the herbal extract and standard had shown increased levels of SOD, GSH, and catalase, whereas levels of SGOT, SGPT, total glucose, HMG-CoA, lipase, amylase, and the percentage of malon-dialdehyde were decreased when compared with the high-fat diet-fed rats. Body weight and food intake in the treated groups were significantly lower than that in the model control.ConclusionThe present study showed that an ethanolic extract of Crotalaria juncea L. influences several blood lipid and metabolic parameters in rats, suggesting a potential benefit as an antihypercholesterolemic agent.

English

Ambrex, a polyherbal formulation was tested for its antihypercholesterolemic effect against experimentally induced hypercholesterolemia in rats. Alteration in the levels of serum marker enzymes, alanine amino transferase (ALT), aspartate amino transferase (AST), lactate dehydrogenase (LDH), alkaline phosphatase (ALP) and general biochemical parameters glucose, protein, urea and creatinine were tested in both treated and untreated groups. Rats treated with high fat diet for four weeks exhibited hypercholesterolemia. Oral administration of ambrex at a dose of 40 mg/kg body weight significantly decreased serum total cholesterol, triglycerides and low density lipoproteins (LDL) levels and there was a concomitant increase in serum high density lipoprotein (HDL) levels. Atherogenic index of the ambrex treated group showed marked improvement compared to other groups. Studies established that ambrex might be a safe drug that exhibits negligible toxic effects on liver and kidney functions under experimental condition. The histopathalogical observation of liver tissue also confirms the same. The results shows the ambrex possess considerable antihypercholesterolemic activity compared with standard drug, atorvastatin. Key words: Hypercholesterolemia, amber, Withania somnifera, atorvastatin.

Antihypercholesterolemic Effect of Macrotyloma uniflorum (Lam.) Verdc (Fabaceae) Extract on High-Fat Diet-Induced Hypercholesterolemia in Sprague-Dawley Rats

ABSTRACTObjective: The present study evaluated the antihypercholesterolemic effect of Macrotyloma uniflorum (Lam.) Verdc extract by examining its effects on food consumption, weight gain, serum lipid profile, serum glutamate oxaloacetate transamianse (SGOT), serum glutamate pyruvate transaminase (SGPT), and body fat in rats. Methods: Animals were fed with experimental high-fat diets (HFD) that contain 10% lard plus, 5% corn oil. Food intake and body weight were measured. Serum samples were analyzed for the estimation of lipid profiles and other biochemical parameters by using kits. Results: The consumption of ethanol and water extract of experimental plant for 5 weeks caused the decrease in total cholesterol (TC), triglycerides, low-density lipoprotein (LDL), very low density lipoprotein (VLDL) SGOT and SGPT significantly (p < .01), and increase in high-density lipoprotein (HDL) significantly (p < .01). The rat group fed with HFD has shown higher serum TC, TG, LDL, VLDL, SGOT, SGPT, and lower HDL than in the normal control group (p < .01). Fecal excretion of cholesterol in the ethanol extract-treated groups was significantly higher than those treated with water extract. Body weight in the water extract-treated groups was significantly lower than that in the ethanol extract-treated groups. Conclusion: This study demonstrated that M. uniflorum (Lam.) Verdc extracts have strong activities against hypercholesterolemia and obesity suggesting a potential usefulness of M. uniflorum (Lam.) Verdc extracts as functional ingredients.

Anti-Hypercholesterolemic and Hepatoprotective effect of Aqueous Leaf Extract of Moringa oleifera in Rats fed with Thermoxidized Palm Oil Diet

In the impact of Moringa oleifera leaf extract on the lipid profile and key liver function enzymes of female rats fed with palm oil diets, twenty-five (25) female rats were divided into five groups (A-E). The rats in group A served as the control, fed with rat chow and water ad libitum, group B (FPO) and C (TPO) were fed with Fresh palm oil and thermoxidized palm oil diet respectively, while group D (FPO + MO) and E (TPO+MO) were fed with fresh palm oil diet and thermoxidized oil with 600mg/kgbody weight of Moringa leaf extract added in each respectively. Each of the palm oil diet contained 15 %( w/w) thermoxidized or fresh palm oil. At the end of 28 days administration, blood samples were collected for the analysis of lipid profile and key liver function enzymes. The result showed that HDL was significantly (P<0.05) increased in all the test samples except TPO, while LDL followed a reverse trend. The Serum alanine amino tranferase (ALT), aspartate amino transferase (AST) and alkaline phosphatase (ALP) were significantly (P<0.05) reduced in all the test samples except TPO which was significantly (P<0.05) increased. Serum gamma glutamyl transpeptidase (GGT) showed decrease in all the test samples except TPO which was significantly (P<0.05) increased. The results indicate that Moringa oleifera has anti-hypercholesterolemic effect. The serum enzyme levels also show that the liver tissue integrity was stabilized by the addition of Moringa oleifera leaf extract to thermoxidized palm oil diet hence its hepatoprotective activity.

Antihypercholesterolemic effect of Bacopa monniera linn. on high cholesterol diet induced hypercholesterolemia in rats

ObjectiveTo explore the effect of alcoholic extract of Bacopa monniera (AEBM) on high cholesterol diet-induced rats. MethodsThe shade-dried and coarsely powdered whole plant material (Bacopa monniera) was extracted with 90% ethanol, finally filtered and dried in vacuum pump. The experimental rats were divided into 4 groups: control (group-I), Rats fed with hypercholesterolemic diet (HCD) for 45 days [4% cholesterol (w/w) and 1% cholic acid], Rats fed with HCD for 45 days+AEBM (40mg/kg, body weight/day orally) for last 30 days (group-III) and AEBM alone (group-IV). Blood and tissues (Aorta) were removed to ice cold containers for various biochemical and histological analysis. ResultsAEBM treatment significantly decreased the levels of TC, TG, PL, LDL, VLDL, atherogenic index, LDL/HDL ratio, and TC/HDL ratio but significantly increased the level of HDL when compared to HCD induced rats. Activities on liver antioxidant status (SOD, CAT, GPx, GR, GST) were significantly raised with concomitant reduction in the level of LPO were obtained in AEBM treated rats when compared to HCD rats. Treatment with AEBM significantly lowered the activity of SGOT, LDH and CPK. Histopathology of aorta of cholesterol fed rat showed intimal thickening and foam cell deposition were noted. ConclusionsThese results suggests that AEBM extended protection against various biochemical changes and aortic pathology in hypercholesterolemic rats. Thus the plant may therefore be useful for therapeutic treatment of clinical conditions associated hypercholesterolemia.

Investigating the effectiveness of different tea types from various thyme kinds (Origanum onites, Thymbra spicata and Satureja cuneifolia) on anemia and anticholesterolemic activity

In a study on villagers settled on the outskirts of the Taurus Mountains and whose source of living is thyme, it was revealed that the villagers excessively consumed thyme by adding it to their tea and many of their foods; high incidences of anemia was found among these villagers. In this study, 42 male adult Wistar albino rats weighing 200-250g were used. The rats were divided to six equal groups as follows: control, cholesterol (Chol), 80mg/kg Origanum onites Labiatae (OOL), 80mg/kg Thymbra spicata Labiatae (TSL), 80mg/kg Satureja cuneifolia Labiatae (SCL), and 160mg/kg TSL, and each group consisted of seven rats. The control group was fed with normal pellet feed. The Chol group and all the other groups, except for the control group, were fed with 2% cholesterol-containing pellet feed. Physiological serum of 4ml was given to the control and Chol group, wheile 80 mg/kg of thymes tea was given to the OOL group, TSL group, and SCL group, and 160mg/kg of thymes tea was given to the TSL group by means of a gavage for 30days. In the blood samples, the hematologic parameters and the biochemical parameters of serum glucose, blood urea nitrogen, creatinine, total protein, albumin, iron (I), total iron-binding capacity, aminotransferase aspartate, alanine aminotransferase, alkaline phosphatase, lactate dehydrogenase, total cholesterol, low-density lipoprotein (LDL) cholesterol, high-density lipoprotein cholesterol, triglyceride, and oxidized LDL levels were examined. The kidney and liver tissues were examined histopathologically. The results of the study showed that different types of thymes had an antihypercholesterolemic effect. In addition to the anemic effect detected in group TSL and the mild granular degeneration found in the liver of 80mg/kg SCL group, distinct granular degeneration was observed in 160mg/kg TSL group.

김치에서 분리한 Lactobacillus plantarum CIB 001 급여가 고콜레스테롤혈증 흰쥐의 지질대사에 미치는 영향

The aim of the present study was to assess the anti-hypercholesterolemic effect of bile salt hydrolase-producing Lactobacillus plantarum CIB 001 (KCTC 11717 bp) in rats fed a high-cholesterol diet. Four treatment groups of rats (n

The medicinal value of honey: a review on its benefits to human health, with a special focus on its effects on glycemic regulation

Honey, a natural substance produced by honeybees, is composed of a complex mixture of carbohydrates, water, and a small amount of proteins, vitamins, minerals, and phenolic compounds. Fructose, glucose and maltose are among the various types of sugars present in honey. Used for millennia as both food and medicine, honey has been associated with improved antioxidant capacity, modulation of the immune system, antimicrobial activities, influence on lipid values (through antihypercholesterolemic effects) and regulation of glycemic responses, among other benefits. The aim of this article was to review the effects of natural honey intake on human health, with particular reference to its influence on glycemic regulation. Several studies have focused on the potential use of honey as a nutritional supplement for healthy individuals and for those with impaired glucose tolerance, diabetes, and their related comorbidities. Such investigations have found that, compared to glucose and sucrose, the consumption of honey decreases glycemic levels and blood lipids in healthy, diabetic and hyperlipidemic individuals. Moreover, long periods of honey intake seem to reduce fasting glucose levels in humans, suggesting that honey consumption influences plasma glucose regulation, mainly through a normo- or hypoglycemic effect. Therefore, honey may be proposed as a nutritional dietary supplement for healthy individuals and for those suffering from alterations in glycemic regulation