The rising concerns regarding the presence of antibiotic residues in food commodities underscore the imperative need for the establishment of methods that are both rapid and precise for their identification. Specifically, given the widespread use and subsequent likelihood of residues of antibiotics such as colistin being found in various meat products, there is an urgency to address this. Even though colistin, a peptide antibiotic, has been prohibited for human consumption, its use in veterinary medicine and as a livestock feed additive remains prevalent. Our current study elaborates on the development of an immunosensor created through the immobilization of anti-colistin antibodies onto a surface composed of MWCNTs and Al₂O₃. This was achieved using a screen-printed electrode. The immunosensor exhibited the ability to generate electrochemical responses upon exposure to chicken liver samples treated with colistin. Responses were recorded using cyclic voltammetry (CV). To ensure accuracy, extensive characterization of the electrode’s surface was carried out at each fabrication stage using Field Emission Scanning Electron Microscopy (FESEM), Fourier Transform Infrared Spectroscopy (FTIR), and Electrochemical Impedance Spectroscopy (EIS). The immunosensor showcased a low limit of detection at 0.89 µg.kg⁻¹ and a coefficient of determination R² of 0.91 when evaluated using CV. For a broader perspective, the immunosensor’s performance was benchmarked against results from commercial chicken liver samples obtained via conventional methodologies. The findings indicate that the innovative MWCNTs/Al₂O₃-based electrode not only functions with remarkable speed but is also highly sensitive. This makes it an ideal choice for applications in biomedical sensing Figure 1
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