Antioxidant Enzyme Activities Research Articles

Ziziphus jujuba (Jujube) in Metabolic Syndrome: From Traditional Medicine to Scientific Validation.

This review evaluates the therapeutic potential of Ziziphus jujuba and its main components in managing complications of metabolic syndrome, including diabetes, dyslipidemia, obesity, and hypertension. The reviewed studies provide evidence supporting the use of Z. jujuba and its main components (lupeol and betulinic acid) as natural treatments for complications of metabolic syndrome. These substances enhance glucose uptake through the activation of signaling pathways such as phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt), reduce hepatic glucose synthesis, and increase glucose uptake by adipocytes and skeletal muscle cells. They also improve insulin sensitivity by modulating AMP-activated protein kinase (AMPK) activity and regulating insulin signaling proteins and glucose transporters. In the field of dyslipidemia, they inhibit triglyceride synthesis, lipid accumulation, and adipogenic enzymes, while influencing key signaling pathways involved in adipogenesis. Z. jujuba and its constituents demonstrate anti-adipogenic effects, inhibiting lipid accumulation and modulating adipogenic enzymes and transcription factors. They also exhibit positive effects on endothelial function and vascular health by enhancing endothelial nitric oxide synthase (eNOS) expression, NO production, and antioxidant enzyme activity. Z. jujuba, lupeol, and betulinic acid hold promise as natural treatments for complications of metabolic syndrome. They improve glucose metabolism, insulin sensitivity, and lipid profiles while exerting anti-adipogenic effects and enhancing endothelial function. However, further research is needed to elucidate the mechanisms and confirm their efficacy in clinical trials. These natural compounds offer potential as alternative therapies for metabolic disorders and contribute to the growing body of evidence supporting the use of natural medicines in their management.

Polyvinyl chloride microplastics and drought co-exposure alter rice growth by affecting metabolomics and proteomics

Microplastics, interacting with drought stress, have become threat to crops by altering soil environment. Currently, the effect of combined microplastic and drought stress on crop growth remain poorly understood. In this work, the mechanism of multi-stress responses was investigated under the exposure of polvinylchloride microplastic (PV) and drought (D) individually and in combination (DPV) on rice varieties Hanyou73 and Q280 through proteomics and metabolomic analysis. All treatments negatively affect chlorophyll content, antioxidant enzyme activities, rice grain composition, metabolome and proteomic profiling of both rice varieties. Full rice grain yield was decreased under all treatments except PV treatment in which it was increased in both rice varieties. DPV treatment shows the lowest grain yield and more adverse effects on metabolome by affecting glycerophospholipid metabolism, tryptophan metabolism and alanine, aspartate and glutamate metabolism. Soluble sugar contents were decreased in H73 but in Q280 increased by 159 % under DPV and 123 % in PV treatment, compared to their control group. The results from metabolomics illustrate that glycerophospholipid metabolism is commonly altered in both rice types under all treatments. PV and drought alone and in combination induce extensive alterations in proteomics of rice leaves especially impacting proteins related to binding, translation and photosynthetic process. The results reveal that PV and DPV treatments highly distort the abundance of metabolites and proteins in both rice types, demonstrating that microplastic toxicity effects on rice plants become more severe when combined with drought stress.

Pear twig biochar combined with nitrogen fertilizer regulates morpho-physiological growth, copper uptake and tuber quality of potato (Solanum tuberosum L.) grown in polluted soil

Application of pear twig derived biochar and nitrogen fertilizer is strategic for addressing the challenges posed by copper pollution in soils. Their combined use aims to improve plant health and promote sustainable agricultural practices, which leads to better potato growth and quality. Therefore, this study was carried out to investigate the effects of different levels of pear twig biochar (B0:0, B1:3, B2:5, B3:7% w/w) combined with nitrogen fertilizer (N0:0, N1:150, N2:200, N3:250, N4:300 mg kg-1) on morpho-physiological growth and copper uptake of potato cultivated in Cu polluted soil. Results showed that combined approach of pear twig biochar and nitrogen significantly influenced morpho-physiology, antioxidant enzyme activity, mineral content and tuber quality of potato. B2N3 significantly increased the plant height and chlorophyll in plants as compared to B0N0 (control). Malondialdehyde and proline contents were highest in control; however, maximum reductions in MDA and proline contents were recorded at B2N4 (70.32% and 92.12% at budding stage, respectively) and at B2N3 (82.44% and 91.93% at flowering stage, respectively). Likewise, B2N3 showed maximum reduction in activities of peroxidase (7343.47 and 11077.27 U g-1), catalase (1184.98 and 165.64 U g-1) and superoxide dismutase (14.84 and 19.94 U g-1) at budding and flowering stages, respectively. However, lowest contents of soil available Cu (2.03 ± 0.5 μg g-1) and tuber flesh Cu (4.44 ± 0.3 μg g-1) were recorded at B2N3 as compared to control. Interestingly, 7% biochar at all levels of nitrogen exhibited a significant decrease in soil available Cu and tuber flesh Cu. Tuber quality traits were also significantly improved at B2N3 as compared to control. However, future research and field trials can help refine the best practices for integrating these elements in different agricultural systems.

Life on both environment in semi-aquatic frogs: Impact of aquatic microplastic (MP) from MP enrichment to growth, immune function and physiological stress

The pervasive distribution of microplastics (MPs) in aquatic ecosystems presents a significant threat to wildlife, with amphibians being particularly vulnerable due to their complex life cycles and ecological roles. This study investigates physiological and ecological impacts of aquatic MP exposure on juvenile black-spotted pond frogs (Pelophylax nigromaculatus), focusing on juvenile frog stage, history of life after metamorphosis. MP examinations in the intestine and body revealed accumulation primarily in the gastrointestinal tracts without evidence of systemic distribution. Experimental exposure to different concentrations of MPs demonstrated adverse effects on growth, physiological stress, and immune function. Notably, higher MP concentrations led to significant reductions in growth and innate immunity, indicative of compromised health. High concentrations of MPs were associated with elevated levels of corticosterone and antioxidant enzymes, indicating physiological stress. However, there was no evidence of extreme hormonal surges or imbalances in antioxidant enzyme activity, suggesting that amphibians were able to effectively cope with the levels of MPs used in the study. Changes in gastrointestinal morphology and fecal microbiota composition were observed, reflecting response of metabolic adaptation to MP exposure. At low concentrations of MPs, adaptive changes in digestive tract morphology and the maintenance of gut microbiota balance were observed, indicating that the frogs were able to manage the exposure below a certain threshold. In contrast, high concentrations of MPs had clear negative effects on amphibians, which could impact biodiversity and ecosystem stability. These findings also suggest that MPs may trigger adaptive responses at lower concentrations, while still posing significant environmental risks at higher levels.

Tolerance and phytoremediation capacity of atrazine and S-metolachlor by two duckweeds.

The phytotoxicity and removal of atrazine and S-metolachlor in sterile duckweed systems were estimated in this study. Herbicides were added at environmentally relevant ranges: 0-400µg/L for atrazine or 0-200µg/L for S-metolachlor in systems with Spirodela polyrhiza or Lemna minor. Toxicity biomarkers, i.e., changes in plant biomass, surface area, chlorophyll fluorescence parameters, pigments, lipid peroxidation, protein concentration, and antioxidative enzyme activities in plants were estimated after 7days. S. polyrhiza (RGRbiomass-EC50 = 164.8µg/L) was more tolerant to atrazine than L. minor (RGRbiomass-EC50 = 101.0µg/L). Atrazine caused damage to photosystem II (ΦM), a reduction in electron transport between PSII and PSI (Φ'M), as well as disruption in energy distribution pathways (decrease in qPrel and increase in UQFrel), most prominently in L. minor. However, L. minor (RGRbiomass-EC50 = 128.9µg/L) was more tolerant to S-metolachlor than S. polyrhiza (RGRbiomass-EC50 = 15.5μg/L). The highest sensitivity of S. polyrhiza to S-metolachlor was attributed to a decrease in absorbed energy used in photochemistry (qPrel) and an increase in lipid peroxidation, indicating that S. polyrhiza plants were experiencing oxidative stress. Residual pesticide analysis in the water after seven days allowed us to conclude that plants were responsible for reducing up to 16.5% of atrazine and 28.7% of S-metolachlor in the duckweed system. S. polyrhiza showed higher atrazine phytoremediation capacity than L. minor. S. polyrhiza was more efficient at an environmentally relevant concentration of S-metolachlor (25μg/L) and L. minor at higher concentrations (200μg/L).

The modulation of proteomics and antioxidant stress is involved in the effect of nitazoxanide against Japanese encephalitis virus in vitro

Japanese encephalitis virus (JEV) is a significant circulating arbovirus flavivirus and the primary cause of viral encephalitis in Asia. Previous studies have demonstrated that nitazoxanide (NTZ), an antiparasitic gastroenteritis medication classified as a thiazolide, exhibits efficacy against JEV both in vitro and in vivo. To explore the potential antiviral mechanisms, we employed Tandem Mass Tag (TMT)-based quantitative proteomics to identify differentially expressed proteins (DEPs) among three groups: Blank cell group, JEV-infected cell group, and JEV-infected cells treated with NTZ. Our analysis revealed that NTZ treatment led to the upregulation of 30 DEPs and downregulation of 54 DEPs in JEV-infected cells. Enrichment analysis using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) indicated that these DEPs are involved in various biological processes and signaling pathways, including transport, localization, response to wounding, P53 pathway activation, and fatty acid metabolism-related pathways. Moreover, we observed that the expression trend of TMX2, a protein associated with redox homeostasis, was consistent with findings from TMT-based quantitative proteomics. Further investigations into reactive oxygen species (ROS), mitochondrial membrane potential, antioxidant enzyme activity, and the KEAP1-NRF2 pathway demonstrated that NTZ effectively regulates the KEAP1-NRF2 pathway while suppressing oxidative stress induced by JEV infection. In conclusion, the proteomic data along with antioxidant stress results presented herein provide a foundational basis for further research into the molecular mechanisms and potential targets of NTZ against JEV.

Synergistic Benefits of Dietary Silymarin and Selenium on Growth, Immune Functions, Antioxidants, and Gut/Liver Health of Thinlip Mullet (Liza ramada) Juveniles

Abstract This study investigates the synergistic impact of silymarin (SI) levels combined with inorganic selenium (sodium selenite: Se) on growth, feed utilization, biochemical parameters, antioxidants, innate immunity, intestinal and liver histology, and gene expression of thinlip mullet (Liza ramada) juveniles. The experimental design involved thinlip mullets initially weighing 3.5±0.13 g, distributed in a completely randomized design with 30 fish per hapa (0.5 × 0.5 × 1 m), and conducted in triplicate over 60 days. Seven experimental diets were employed, including a control (without SI and Se supplementation), a negative control (with only Se supplementation), and four treatments with varying levels of silymarin (250, 450, 650, 850 mg/kg) alongside selenium (0.5 mg/kg diet). The growth performance results highlighted significant enhancements in final body weight, weight gain, and specific growth rate, particularly in the SI 850 mg/kg + Se treatment. Survival rates, feed intake, and feed conversion ratios showed positive trends across the SI-Se supplemented groups. Biochemical profiles of serum exhibited that the control diet induced elevated concentrations of glucose, cholesterol, alanine aminotransferase, aspartate aminotransferase, and urea, while Se or SI supplementation significantly mitigated these levels, with the lowest concentrations observed in the SI-Se supplemented groups. Moreover, SI supplementation increased serum protein content. Antioxidant enzyme activities, represented by superoxide dismutase (SOD), catalase (CAT), and catalase (GPx), demonstrated notable improvements in the SI-Se fortified groups, with significantly elevated GPx activity compared to the Se-supplemented and control groups. Immune system responses, including lysozyme, bactericidal, nitro-blue tetrazolium (NBT%), and serum alternative complement pathway (ACH50) activities, were highest in the SI-Se augmented groups. SI and Se in L. ramada reduce liver pro-inflammatory gene expression (IL-1 β, hepcidin) vs. control group. Histological examinations of the intestine and liver depicted structural enhancements, especially at moderate and high levels of SI with Se supplementation. The results indicate improved intestinal villi morphology and hepatic architecture, supporting the positive influence of dietary treatments on the health of thinlip mullet juveniles. In conclusion, the combined supplementation of SI at 850 mg/kg diet and Se at 0.5 mg/kg diet positively influenced the growth, biochemical profiles, antioxidant status, immune responses, gene expression, and histological integrity of thinlip mullet juveniles, providing valuable insights for optimizing aquafeed formulations.

Assessing the impact of weathered polystyrene collected from the marine environment on oxidative stress responses in Zophobas morio larvae: A preliminary study

Foamed polystyrene (PS) is a prevalent material in consumer products and thus represents one of the largest constituents of marine litter. PS may interact with and be ingested by various organisms, including insects, which are starting to be used in biodegradation of plastic wastes. This study examines the physiological performances and the potential oxidative stress related to dietary environmental PS exposure of Z. morio larvae. Experimental groups were fed different diets: bran and oatmeal (control), weathered PS collected from the marine environment, and virgin PS. Over a 30-day feeding period, larvae growth, survival, and PS consumption were measured, together with antioxidant enzymatic activities and gene expression profiles. Results showed that PS ingestion supports larval growth similarly to bran, but the two PS-fed groups consumed a different amount of plastic, suggesting that weathered PS might affect the patterns of PS consumption by larvae. We denoted that PS consumption was associated with a decline in total antioxidant capacity, which showed the highest decrease in the environmental PS group. Marked increases in oxidative stress biomarkers such as superoxide dismutase (SOD) activity/mRNA levels and malondialdehyde content were also observed in the environmental PS-fed group, indicating an elevated oxidative stress response, probably due to pollutants adsorbed/absorbed on PS. Differently to SOD, catalase at both mRNA and enzyme activity levels was found to be significantly reduced by PS digestion, regardless of PS type. The mRNA levels of glutathione s-transferase (GST) were significantly higher in the environmental PS-fed larvae compared to the virgin PS-fed group. In addition, Principal Component Analysis clearly distinguished between larvae fed different PS types, highlighting the enhanced oxidative stress caused by the ingestion of PS collected from marine environments. These results support the potential of insects in plastic biodegradation processes but also demonstrate the health hazards of using environmental PS as principal dietary component in Z. morio larvae, thus questioning their use in downstream productions.

Chromium uptake and its impact on antioxidant level, photosynthetic machinery, and related gene expression in Brassica napus cultivars.

The development of heavy metals, particularly chromium (Cr)-tolerant crop cultivars, is hampered due to lack of understanding of the mechanisms behind Cr stress tolerance. In this study, two Brassica napus cultivars, ZS758 and ZD622, were compared for Cr stress resistance by using the chlorophyll a fluorescence technique and biochemical characteristics. In both cultivars, Cr stress dramatically decreased PSII and PSI efficiency, biomass accumulation, and antioxidant enzyme levels. Although, cultivar ZS758 showed reduction in oxidative stress by decreasing the production of reactive oxygen species (ROS) in terms of reduced H2O2 and MDA content and increased enzymatic activities of key antioxidants enzymes including SOD, APX, CAT, and POD activities that play a crucial role in the regulation of numerous transcriptional pathways involved in oxidative stress responses. Higher non-photochemical quenching (NPQ) and QY were found in tolerant ZS758 cultivar under Cr stress, indicating that tolerant cultivar had a greater capacity to preserve PSII activity under Cr stress by enhancing heat dissipation as a photo-protective component of NPQ. Lower PSI activity and electron transfer from PSII were confirmed by lower PSI efficiency and higher donor end limitation of PSI in both rapeseed cultivars. The Cr concentration was greater in the ZD622 as compared to ZS758, which affected the mineral nutrients profile and damaged the cellular ultrastructure and related gene expression levels. However, current study suggest that cultivar ZS758 is more resistant to Cr stress than ZD622 due to improved metabolism and structural integrity and Cr stress tolerance that is linked with the increased PSII activity, NPQ, and antioxidant potential; these physiological characteristics can be exploited to select cultivars for Cr stress tolerance.

Assessing the impact of arsenite and arsenate on Sarcodia suae: a tale of two toxicities.

Inorganic arsenic (iAs), which predominantly occurs as arsenite (As3+) and arsenate (As5+) in natural water, is primarily accumulated by seaweed in marine environments. However, the detailed mechanisms through which As3+ and As5+ affect the physiological processes of these organisms remain largely unknown. This study focused on evaluating the toxicological effects of As3+ and As5+ on the seaweed Sarcodia suae. Exposure to As3+ and As5+ resulted in IC50 values of 401.5 ± 9.4 μg L-1 and 975.8 ± 13 μg L-1, respectively. Morphological alterations and a reduction in phycoerythrin content were observed, particularly under As3+ exposure, with increased lipid peroxidation as evidenced by higher malondialdehyde levels. Exposure to As3+ also elevated the production of superoxide radicals, while decreasing hydrogen peroxide levels specifically in the presence of As3+. The induction of antioxidative enzyme activities, namely superoxide dismutase, catalase, glutathione reductase, and ascorbate peroxidase was observed, signaling an adaptive response to iAs-induced oxidative stress. Moreover, levels of the antioxidants ascorbate and glutathione were elevated post-exposure, especially in response to As3+. Additionally, bioaccumulation of arsenic was significantly higher in the As3+ compared to As5+. Collectively, the data suggest that As3+ imposes greater adverse effects and oxidative stress to S. suae, which responds by adjusting its antioxidative defense mechanisms to mitigate oxidative stress.

The effects of Selenium phytotoxicity on two wheat (Triticum aestivum) cultivars differing in Se tolerance and the role of antioxidant enzymes in the tolerance mechanism

Abstract Wheat seedlings were hydroponically grown in Hoagland solution containing various levels of Se. Tolerance response to Se toxicity was investigated by determining the level of thiobarbituric acid reactive substance (TBARS), proline and chlorophyll content, the growth parameters, and the activity of antioxidant enzymes. The toxic level of Se treatment significantly retarded the seedling growth. A substantial amount of proline accumulation was also observed in response to toxic Se concentration, but it was more pronounced in putative-sensitive cultivars. Chlorophyll content significantly decreased in Se-intoxicated seedlings and increased at the lowest Se dose in both cultivars. Severe and mild chlorosis was observed in putative-sensitive and tolerant cultivars at the highest Se level. Alterations in the activities of glutathione reductase (GR, 1.6.4.2), glutathione S transferase (GST, 2.5.1.18), guaiacol peroxidase (GPX, 1.11.1.7), catalase (CAT, 1.11.1.6), and ascorbate peroxidase (APX, 1.11.1.11) and superoxide dismutase (SOD, EC 1.15.1.1) were determined. TBAR level did not significantly increase in putative tolerant cultivars as an indicator of membrane lipid peroxidation. However, a significant increase was observed in putative-sensitive cultivars in response to higher selenium concentrations. In higher Se treatment groups, CAT and GST activities significantly increased in putative Se tolerant cultivars. However, excluding SOD, the activity of all the studied enzymes was increased considerably in putative-sensitive cultivars in a dose-dependent manner. Higher antioxidant enzyme activities and a substantial amount of proline accumulation did not significantly contribute to overcoming Se phytotoxicity in wheat seedlings grown in media supplemented with toxic selenium levels.

Tenebrio molitor (Mealworm) protein as a sustainable dietary strategy to improve health span in D-galactose-induced aged mice

Aging is an irreversible and continuous biological process involving intricate and interconnected mechanisms. The present work is focused on unravelling the anti-aging mechanisms of mealworm protein and protein-enriched fruit bar and vegetable soup in D-galactose-induced aged mice. Mealworm protein and enriched products significantly enhanced body weight, organ indices, and gut health. Behavioral assessments reflected enhanced neuroprotective effects. Mealworm protein and its enriched products demonstrated protective effects through anti-inflammatory activity with the highest reduction of TNFα (17.1 %), IL-6 (55.5 %), and IL-1β (75.1 %) levels and upregulated the anti-inflammatory marker (IL-4). Gene expression studies confirmed the induction of anti-aging effects by promoting metabolism, reducing cellular senescence, and enhancing anti-oxidant enzyme activity. The treatments extended telomere lengths by 3–4 times, further affirming the potential anti-aging efficacy of mealworm protein and its enriched products. Mealworm protein demonstrated positive effects on weight gain, anti-inflammatory responses, and telomere length; while fruit and vegetable products enhanced antioxidant activity, and positively influenced gut health. Further, a synergistic effect was observed by combining them, which resulted in improved overall anti-aging effect. The present work provides valuable insights into the multifaceted anti-aging mechanisms associated with mealworm protein and enriched products, highlighting their potential as functional foods with significant health-promoting effects.

Silicon dioxide and selenium nanoparticles enhance vase life and physiological quality in black magic roses

In contemporary floriculture, particularly within the cut flower industry, there is a burgeoning interest in innovative methodologies aimed at enhancing the aesthetic appeal and prolonging the postharvest longevity of floral specimens. Within this context, the application of nanotechnology, specifically the utilization of silicon and selenium nanoparticles, has emerged as a promising approach for augmenting the qualitative attributes and extending the vase life of cut roses. This study evaluated the impact of silicon dioxide (SiO2-NPs) and selenium nanoparticles (Se-NPs) in preservative solutions on the physio-chemical properties of ‘Black Magic’ roses. Preservative solutions were formulated with varying concentrations of SiO2-NPs (25 and 50 mg L−1) and Se-NPs (10 and 20 mg L−1), supplemented with a continuous treatment of 3% sucrose. Roses treated with 20 mg L−1 Se-NPs exhibited the lowest relative water loss, highest solution uptake, maximum photochemical performance of PSII (Fv/Fm), and elevated antioxidative enzyme activities. The upward trajectory of hydrogen peroxide (H2O2) and malondialdehyde (MDA) levels in petals was mitigated by different levels of SiO2 and Se-NPs, with the lowest H2O2 and MDA observed in preservatives containing 50 mg L−1 SiO2- and 20 mg L−1 Se-NPs at the 15th day, surpassing controls and other treatments. Extended vase life and a substantial enhancement in antioxidative capacity were noted under Se and Si nanoparticles in preservatives. The levels of total phenols, flavonoids, and anthocyanin increased during the vase period, particularly in the 50 and 20 mg L−1 Se-NPs and SiO2-NPs. Petal carbohydrate exhibited a declining trend throughout the longevity, with reductions of 8% and 66% observed in 20 mg L−1 Se-NPs and controls, respectively. The longest vase life was achieved with Se-NPs (20 mg L−1), followed by SiO2-NPs (50 mg L−1) up to 16.6 and 15th days, respectively. These findings highlight the significant potential of SiO2- and Se-NPs in enhancing the vase life and physiological qualities of ‘Black Magic’ roses, with SiO2-NPs showing broad-spectrum efficacy.

Unpredictable chronic mild stress induced anxio-depressive disorders and enterobacteria dysbiosis: Potential protective effects of Detariummicrocarpum

Ethnopharmacological relevanceDetarium microcarpum Guill. & Perr. is used traditionally in Far North Cameroun to treat stomach aches, anxiety, epilepsy, and other mental disorders. Aim of the studyEvaluate the anxiolytic and antidepressant-like effects of D. microcarpum (DM) in unpredictable chronic mild stress (UCMS) model of depression in male rats and its impact on fecal enterobacteria of stressed rats. Materials and methodsRats were handled daily (control) or subjected to the UCMS procedure for 42 days. Anxiety-like behaviors were assessed using the light and dark box test (LBD) and the open field test (OFT). Depressive-like behaviors were assessed using the forced swimming test (FST), the sucrose preference test (SPT), and the novelty suppressed feeding test (NSFT). Feces were then collected, followed by blood, brain, and duodenum sections after sacrifice. Monoamine levels, pro-inflammatory cytokines, oxidative stress factors, and nitrosative stress were assessed. Feces were introduced into Hectoen enteric agar for the identification of enterobacteria. An in vitro growth test was performed. ResultsThe DM ethanolic extract has significantly increased the time spent in the light box, in the LBD, and in the center area of the OFT. Moreover, the extract has significantly reduced the preference for sucrose in the SPT, the time of immobility in the FST, and the latency period to consume the pet in the NSFT. DM extract has significantly reduced serum cortisol levels. It also significantly decreased the pro-inflammatory cytokines TNF-α and Il-1β in both brain and duodenum homogenate. DM has increased the brain's serotonin, GABA, and dopamine levels. The DM extract also decreased the MDA and nitrite levels. It also increased the SOD and CAT activities in both brain and duodenal homogenate. Histologically, the DM extract restored the cell's density in hippocampi sections and prevented gut inflammation and peroxidation characterizing leaky gut syndrome. DM extract has no effect on the growth of enterobacteria species isolated in vitro. ConclusionThe ethanolic extract of DM would have anxiolytic and antidepressant effects via the modulation of the HPA axis, brain antioxidant enzyme activities, inflammation, and nitrosative stress. Moreover, it could act by preventing leaky gut syndrome.

Selenium-containing peptides as effective alleviators for low-level lead induced neural damage

The TSeMMM and SeMDPGQQ, two selenium-containing peptides derived from Se-enriched rice protein hydrolysates, have previously been demonstrated to show neuroprotective properties against high-dose lead exposure. However, the potential toxicological effects of these functional peptides on low-dose lead-induced neurotoxicity remain poorly understood. The present study aimed to investigate the protective mechanism of two selenium-containing peptides in mitigating low-dose Pb2+-induced damage in HT22 cells. Results showed that treatment with 40 μmol/L Pb2+ significantly reduced cell viability and increased apoptosis, both of which were alleviated by 40 μmol/L TSeMMM and SeMDPGQQ treatment. Furthermore, two peptides exhibited antioxidant potentials by reducing reactive oxygen species levels to 32.19% and 79.29% of the model group, respectively. They also enhanced antioxidant enzyme activities and mitigated oxidative stress through activation of the Keap1/Nrf2 pathway. Additionally, two peptides improved mitochondrial function by elevating mitochondrial membrane potential, ATP production while dramatically reducing reactive oxygen species levels. Interestingly, TSeMMM exhibited superior neurointerventional effects compared to SeMDPGQQ. The RNA sequencing analysis revealed that TSeMMM modulated gene expressions related to oxidative stress and neuroprotective function. The study provides insights into the protective mechanism underlying selenium-containing peptides for mitigating neuronal damage.

Antioxidant Features of Humic Products by ABTS Assay.

Oxidative stress plays a pivotal role in driving immunosenescence by disrupting cellular homeostasis and impairing immune function. Humic substances exhibit scavenging activity against reactive oxygen species (ROS), inhibit ROS generation via metal chelation, and modulate endogenous antioxidant enzyme activity. Additionally, humic substances display anti-inflammatory effects, further supporting cellular redox balance. Given their antioxidant activity, humic substances hold promise as natural compounds for mitigating oxidative stress-associated immunosenescence. Here we describe the evaluation of antioxidant capacities of humic products by ABTS spectrophotometric assay.

The transcription factor MfbHLH104 from Myrothamnus flabellifolia promotes drought tolerance of Arabidopsis thaliana by enhancing stability of the photosynthesis system

The resurrection plant Myrothamnus flabellifolia can survive extreme drought and desiccation conditions, and quickly recover after rewatering. However, little is known about the mechanism underlying the drought tolerance of M. flabellifolia. In this study, MfbHLH104 was cloned and introduced into Arabidopsis thaliana due to the lack of a transgenic system for M. flabellifolia. MfbHLH104 is localized in the nucleus. Its N-terminal region has transactivation ability in yeast, and the C-terminal region may inhibit the transactivation ability. Overexpressing MfbHLH104 significantly increased drought and salt tolerance of A. thaliana at both seedling and adult stages. It enhanced leaf water retention capacity by decreasing water loss rate and increasing drought- and abscisic acid (ABA) -induced stomatal closure. Additionally, it boosted osmolyte accumulation and ROS scavenging ability by up-regulating genes associated with osmolyte biosynthesis and antioxidant enzymes, and enhancing antioxidant enzyme activities. The expression of ABA-responsive genes were also promoted by MfbHLH104. Remarkably, RNA-seq analysis indicated that MfbHLH104 significantly up-regulated 32 genes (FDR < 0.05 and fold change ≥1.5) involved in photosynthesis related pathways (KEGG pathway No: ko00195, ko00196) under drought, which account for 18.7% of the total up-regulated genes and the most enriched KEGG pathways. This result suggested that it may help to maintain the stability of the photosynthesis system under drought conditions.

Exogenous glycine betaine maintains postharvest blueberry quality by modulating antioxidant capacity and energy metabolism

Blueberries are susceptible to decay and spoilage due to increased respiration rates and metabolism after harvest. The aim of this study was to examine the impact of postharvest application of exogenous glycine betaine (GB) on blueberry storage quality, antioxidant system, and energy metabolism in order to enhance understanding of the regulatory mechanisms of GB on blueberry quality. Fresh blueberries were soaked for 5 min in different concentrations of GB. Preliminary pre-tests revealed that 10 mmol L−1 GB treatment significantly retarded the quality deterioration, decay and dent rate, while increasing the firmness and soluble solid content. Concurrently, exogenous GB treatment resulted in an increase in the content of anthocyanosides, total flavonoids, total phenols, and reduced glutathione, also exhibited higher antioxidant enzyme activities, including superoxide dismutase, catalase, ascorbate peroxidase, polyphenol oxidase, phenylalanine ammonia-lyase, glutathione reductase, dehydroascorbate reductase, and monodehydroascorbate reductase. In GB-treated blueberries, endogenous GB content was significantly higher. The hydrogen peroxide value, superoxide anion, and malondialdehyde content, and higher levels of free radical scavenging rate (DPPH), which significantly improved antioxidant capacity. Meanwhile, succinate dehydrogenase, cytochrome C oxidase, H+-ATPase, and Ca2+-ATPase, decreased and slowed the senescence of blueberries, the ATP content and energy charge were observed to be elevated. In summary, exogenous GB treatment maintained the quality of post-harvest blueberries by increasing their antioxidant capacity and inhibiting the decrease in the activity of energy metabolism-related enzymes. The results indicate that 10 mmol L−1 GB treatment may be an efficacious approach for maintaining the quality and prolonging the shelf life of blueberries, offering novel insights into the preservation of this fruit.

Mitigating water deficit stress in lemon balm (Melissa officinalis L.) through integrated soil amendments: A pathway to sustainable agriculture.

Lemon balm (Melissa officinalis L.) is a valuable medicinal plant, but its growth can be significantly impacted by drought stress. This study aimed to mitigate the adverse effects of water deficit stress on lemon balm biomass by integrating poultry manure compost, poultry manure biochar, NPK fertilizer, Trichoderma harzianum, Thiobacillus thioparus, and elemental sulfur as soil amendments. The experiment was conducted in a greenhouse using a completely randomized design with a factorial arrangement, consisting of three replicates. It included a water deficit stress factor at three levels (95-100%, 75-80%, and 55-60% of field capacity) and a soil amendment treatment factor with eleven different fertilizer levels. Treatments included control (no amendment), NPK fertilizer, poultry manure compost, poultry manure biochar, and combinations of these with T. harzianum, T. thioparus, and elemental sulfur under various water deficit levels. Water deficit stress significantly reduced photosynthetic pigments, gas exchange parameters, chlorophyll fluorescence, relative water content, and antioxidant enzyme activity, while increasing membrane permeability and lipid peroxidation in lemon balm plants. However, the integrated application of organic, biological, and chemical amendments mitigated these negative impacts. The combined treatment of poultry manure compost, poultry manure biochar, NPK fertilizer, T. harzianum, T. thioparus, and elemental sulfur was the most effective in improving the morpho-physiological properties (1.97-60%) and biomass (2.31-2.76 times) of lemon balm under water deficit stress. The results demonstrate the potential of this holistic approach to enhance the resilience of lemon balm cultivation in water-scarce environments. The integration of organic, biological, and chemical amendments can contribute to sustainable agricultural practices by improving plant morphological and physiological properties and plant performance under drought conditions.

Effects of 60Coγ-irradiation combined with sodium dehydrogenate on post-harvest preservation and physiological indices of Volvariella volvacea.

Volvariella volvacea is a mushroom known for its high palatability and nutritional value. However, it is susceptible to spoilage thus making it challenging to preserve and keep fresh after harvest, resulting in constraints in long-distance transportation and long-term storage. This study aimed to investigate the feasibility of using irradiation and sodium dehydrogenate (SD) as a preservative in the preservation process of V. volvacea. The effects of three treatments of 0.8 kGy 60Coγ irradiation (B), 0.04% SD (C), combined with 0.04% SD and 0.8 kGy 60Coγ irradiation (A) on the postharvest freshness of V. volvacea were investigated. The assessment indices for V. volvacea, including appearance, browning rate, weight loss, respiration rate, MDA content, antioxidant enzyme activities, vitamin C (Vc), and soluble protein content, were measured and compared. The three treatments were compared to determine the changes in storage time over 7 days post-harvest. The results demonstrated that the hardness of the fruiting body exhibited a significant increase of 81.19%, 97.96% and 168.81% in comparison to the control, B and C, respectively, following the application of the treatment A. Compared to the control group, the soluble protein content was significantly increased by 20.28%. Respiration intensity and browning rate were significantly lower in the control treatment, decreasing by 35.07% and 45.49% respectively. On the 6th day of storage, the activities of SOD and POD increased by 81.06% and 73.71%, respectively, compared to the control, which significantly delayed the senescence of the fruiting bodies. The Vc content was significantly increased by 50.27%, 133.90%, and 101.39% in treatment B, which received 0.8 kGy 60Coγ irradiation alone, compared to the control, treatment A, and treatment C, respectively. The treatment C alone significantly reduced respiratory intensity and MDA variables by 39.55% and 31.01%, respectively, compared to the control. The findings can provide theoretical references and technical support for extending the preservation period of V. volvacea after harvesting by using irradiation and sodium dehydrogenate as a preservative.