Antimutagenic Properties Research Articles

Melipona scutellaris Geopropolis: Chemical Composition and Bioactivity.

Geopropolis has been used in traditional medicine for centuries. In this study, the botanical origin, physicochemical profile, and biological activities of geopropolis from Melipona scutellaris harvested during rainy and dry seasons were investigated. Palynological analysis identified over 50 pollen types, with Schinus terebinthifolius and Cecropia being the predominant types. The analytical results were in line with those reported in the literature. Rainy-season geopropolis exhibited higher total phenol and flavonoid content (determined using High Performance Liquid Chromatography-25.13% and 3.92%, respectively) compared to the dry season (19.30% and 2.09%); the major peaks (naringin, gallic acid, and catechin) were similar among samples. Antioxidant capacity was assessed via DPPH, reducing power, and β-carotene/linoleic acid discoloration assays. Rainy-season samples displayed superior antioxidant activity across methods. Antimicrobial effects were determined using microdilution, while the impact on the cholinesterase enzyme was quantified using 5-thio-2-nitrobenzoic acid accumulation. Anti-inflammatory and antimutagenic activities were assessed through hyaluronidase enzyme inhibition and by utilizing Saccharomyces cerevisiae ATCC-20113 cells. Both samples exhibited anti-inflammatory and antimutagenic properties. Moreover, a significant inhibition of acetylcholinesterase was observed, with IC50 values of 0.35 µg/mL during the rainy season and 0.28 µg/mL during the dry season. Additionally, the geopropolis displayed antimicrobial activity, particularly against Staphylococcus aureus. These findings suggest the therapeutic potential of M. scutellaris geopropolis in the context of inflammatory, oxidative, and infectious diseases.

Black seed oil impact on authentic kefir microbiota

Introduction: Authentic kefir originates from the Caucasus mountains of Eastern Europe and is a fermented milk product made from kefir grains. Authentic kefir contains lactic acid bacteria, acetic acid bacteria and yeasts which provides kefir with numerous health benefits such as anticarcinogenic, antimutagenic and anti-allergic properties. Nigella sativa is a plant known by many regional names such as black cumin and black carraway. Seeds from the plant are processed to produce black seed oil. Black seed oil has many potential health benefits such as antibacterial and antifungal capabilities. In countries including Turkey and India, black seed oil is commonly added to kefir or yogurt. The purpose of this study was to determine if different concentrations of black seed oil would negatively impact beneficial kefir microorganisms when consumers mix black seed oil into kefir. Results: Black seed oil concentrations of 0%, 0.1%, 1% and 5% were added to milk with kefir grains and incubated. Each mixture was tested for pH, Lactobacillus spp., Lactococcus spp., yeast, and coliform microbial counts. Results indicated that the pH and microbial counts of the control (0%) and 0.1% black seed oil samples were not significantly different (P > 0.05). The pH and microbial count results of 1% black seed oil in kefir indicated slight although not significant inhibition (P > 0.05) as compared to the control and 0.1% black seed oil. The pH and microbial counts of 5% black seed oil were significantly different (P < 0.05) from the other samples indicating inhibition of the kefir microorganisms.Conclusion:Black seed oil inhibited kefir microorganism when added at the rate of 5%.Keywords: Kefir, Black Seed Oil, Lactobacillus spp., Lactococcus spp., Yeast, Probiotic

Mutagenic, Anti-mutagenic, and Cytotoxic Activities of Methanol, Hexane, and Ethyl Acetate Extracts of Zingiber zerumbet (L.) Smith

Background Zingiber zerumbet (L.) Smith (lempoyang) has a traditional application in the treatment of indigestion, worm infestation, loss of appetite, and postpartum conditions. Objectives Extracts of Z. zerumbet (methanol, hexane, and ethyl acetate) were utilized to investigate the mutagenic, anti-mutagenic, and cytotoxic properties. Materials and Methods Initially, a mutagenicity test (Ames test) was conducted, followed by an anti-mutagenicity test, to assess the potential of Z. zerumbet extracts in inhibiting mutagenicity induced by sodium azide and 9-aminoacridine. Furthermore, the cytotoxic ability of the extracts was evaluated using the methyl thiazolyl tetrazolium (MTT) assay. Results The mutagenicity assessment revealed that the methanol extract of Z. zerumbet exhibited a twofold increase in the number of revertants in Salmonella typhimurium strain TA 1537. Similar results were observed for the hexane extract, except at a concentration of 6.25 mg/mL, where no significant increase in revertants was observed. On the other hand, the ethyl acetate extract demonstrated a twofold increase in revertants in S. typhimurium strain TA 1535. Notably, the ethyl acetate extract displayed remarkable anti-mutagenic activity against 9-aminoacridine, while the hexane extract exhibited strong anti-mutagenic activity against sodium azide. Regarding cytotoxicity assessment using the MTT assay, the methanol extract of Z. zerumbet exhibited the highest cytotoxicity with an IC50 value estimated at 388.50 ± 135.75 µg/mL. The hexane and ethyl acetate extracts showed IC50 values of 514.17 ± 135.75 and 589.67 ± 241.67 µg/mL, respectively. Conclusion The extracts displayed both mutagenic and cytotoxic activities. However, they also exhibited promising anti-mutagenic potential, which could be harnessed for cancer prevention purposes.

Electrochemistry of Flavonoids: A Comprehensive Review.

Flavonoids represent a large group of aromatic amino acids that are extensively disseminated in plants. More than six thousand different flavonoids have been isolated and identified. They are important components of the human diet, presenting a broad spectrum of health benefits, including antibacterial, antiviral, antimicrobial, antineoplastic, anti-mutagenic, anti-inflammatory, anti-allergic, immunomodulatory, vasodilatory and cardioprotective properties. They are now considered indispensable compounds in the healthcare, food, pharmaceutical, cosmetic and biotechnology industries. All flavonoids are electroactive, and a relationship between their electron-transfer properties and radical-scavenging activity has been highlighted. This review seeks to provide a comprehensive overview concerning the electron-transfer reactions in flavonoids, from the point of view of their in-vitro antioxidant mode of action. Flavonoid redox behavior is related to the oxidation of the phenolic hydroxy groups present in their structures. The fundamental principles concerning the redox behavior of flavonoids will be described, and the phenol moiety oxidation pathways and the effect of substituents and experimental conditions on flavonoid electrochemical behavior will be discussed. The final sections will focus on the electroanalysis of flavonoids in natural products and their identification in highly complex matrixes, such as fruits, vegetables, beverages, food supplements, pharmaceutical compounds and human body fluids, relevant for food quality control, nutrition, and healthcare research.

Phyllanthus emblica: a comprehensive review of its phytochemical composition and pharmacological properties.

Phyllanthus emblica Linn, a prominent member of the euphorbiaceae family, exhibits extensive distribution across a multitude of tropical and subtropical nations. Referred to as "Balakka" in Indonesia, this plant assumes various names across regions, such as "kimalaka," "balakka," "metengo," "malaka," and "kemloko" in North Sumatra, Ternate, Sundanese, and Java respectively. Phyllanthus emblica thrives in tropical locales like Indonesia, Malaysia, and Thailand, while also making its presence felt in subtropical regions like India, China, Uzbekistan, and Sri Lanka. The fruits of Balakka are enriched with bioactive constituents recognized for their wide-ranging benefits, including antioxidant, anti-aging, anti-cholesterol, anti-diabetic, immunomodulatory, antipyretic, analgesic, anti-inflammatory, chemoprotective, hepatoprotective, cardioprotective, antimutagenic, and antimicrobial properties. Comprising a spectrum of phenolic compounds (such as tannins, phenolic acids, and flavonoids), alkaloids, phytosterols, terpenoids, organic acids, amino acids, and vitamins, the bioactive components of Malacca fruit offer a diverse array of health-promoting attributes. In light of these insights, this review aims to comprehensively examine the pharmacological activities associated with P. emblica and delve into the intricate composition of its phytochemical constituents.

The Antioxidative Effects of Flavones in Hypertensive Disease.

Hypertension is the leading remediable risk factor for cardiovascular morbidity and mortality in the United States. Excess dietary salt consumption, which is a catalyst of hypertension, initiates an inflammatory cascade via activation of antigen-presenting cells (APCs). This pro-inflammatory response is driven primarily by sodium ions (Na+) transporting into APCs by the epithelial sodium channel (ENaC) and subsequent NADPH oxidase activation, leading to high levels of oxidative stress. Oxidative stress, a well-known catalyst for hypertension-related illness development, disturbs redox homeostasis, which ultimately promotes lipid peroxidation, isolevuglandin production and an inflammatory response. Natural medicinal compounds derived from organic materials that are characterized by their anti-inflammatory, anti-oxidative, and anti-mutagenic properties have recently gained traction amongst the pharmacology community due to their therapeutic effects. Flavonoids, a natural phenolic compound, have these therapeutic benefits and can potentially serve as anti-hypertensives. Flavones are a type of flavonoid that have increased anti-inflammatory effects that may allow them to act as therapeutic agents for hypertension, including diosmetin, which is able to induce significant arterial vasodilation in several different animal models. This review will focus on the activity of flavones to illuminate potential preventative and potential therapeutic mechanisms against hypertension.

Antimutagenicity and Antioxidant Activity of Castanea sativa Mill. Bark Extract.

Castanea sativa Mill. (Cs), a plant traditionally employed in nutrition and to treat various respiratory and gastrointestinal infections, possesses cancer chemopreventive characteristics. In particular, Cs bark extract previously demonstrated antiproliferative and pro-apoptotic activities against a leukemic lymphoblastic cell line. Starting from this evidence, the aim of this paper was to investigate the possibility to affect also the earlier phases of the carcinogenic process by evaluating Cs bark extract's antimutagenic properties, in particular using the "In Vitro Mammalian Cell Micronucleus Test" on TK6 cells performed by flow cytometry. For this purpose, since an ideal chemopreventive agent should be virtually nontoxic, the first step was to exclude the extract's genotoxicity. Afterwards, the antimutagenic effect of the extract was evaluated against two known mutagens, the clastogen mitomycin C (MMC) and the aneugen vinblastine (VINB). Our results indicate that Cs bark extract protected cells from MMC-induced damage (micronuclei frequency fold increase reduction from 2.9 to 1.8) but not from VINB. Moreover, we demonstrated that Cs bark extract was a strong antioxidant and significantly reduced MMC-induced ROS levels by over 2 fold. Overall, our research supports the assumption that Cs bark extract can counteract MMC mutagenicity by possibly scavenging ROS production.

Use of probiotic preparations to increase productive qualities in goats: a review

An analytical review of scientific publications on probiotics in goat breeding was carried out. It has been established that the most popular probiotic microorganisms in goat farming are bacteria of the species Bacillus subtilis, Bifidobacterium bifidum, Enterococcus faecium, Lactobacillus acidophilus, Lactobacillus plantarum and single-celled yeast of the species Saccharomyces cerevisiae. Including probiotic microflora in the diet increases the number of bacteria in the rumen contents of goats. Reducing the number of Clostridia and Escherichia coli in animal faeces is achieved by replacing the native faecal microflora with streptococci and lactic acid bacteria. It has been shown that feeding probiotic preparations is accompanied by an improvement in the metabolic status in the body of goats and is expressed by an increase in the concentration of total protein and its fractions, haemoglobin, erythrocytes, macro- and microelements in the blood of animals, and optimisation of lipid metabolism. Probiotics help preserve villi and inhibit inflammation of the intestinal epithelium of animals, exhibit antimutagenic and anticarcinogenic properties, and help reduce putrescine concentration in faeces. Feeding probiotics ensures an increase in daily milk yield, fat and protein content by increasing the digestibility of diet solids, primarily crude fibre. The probiotic potential of some microorganisms is expressed in increased concentrations of unsaturated fatty acids in goat milk and meat with a decrease in the atherogenic index of raw materials. The use of drugs helps to increase the absolute increase in live weight of goat kids against the background of the harmonious development of the physique of young animals due to the production of microbial phytase, a decrease in the redox potential of chyme and an increase in the number of microorganisms that consume lactic acid. Some authors report the absence of a significant effect when using probiotics in feeding lactating animals and raising goat kids.

Phytochemicals and therapeutic use of date palm (Phoenix dactylifera L) – A narrative review

This article provides a summary of phytochemical composition, nutritional value and health benefits of Pheonix Dactylifera L, commonly known as dates. Additionally, the article explores the potential of date fruit to be utilized as a food medicine in the treatment of various human diseases. We conducted a comprehensive review of the existing literature on the biological functions of date palm and its potential health benefits, utilizing databases literature search engines including PubMed, Scopus, Science Direct and google scholar. Regular consumption of date palm fruits (DPFs) may lower the risk of developing various chronic diseases due to its antioxidant, antidiabetic, antibacterial, anticancer and anti-mutagenic properties. Additionally, the pollen of date palm trees contains high levels of protein, fat, and carbohydrates, making it an excellent natural supplement with substantial nutritional value and energy-boosting properties. Date palm components have demonstrated potent antioxidant effects and have played a crucial role in both male and female reproductive systems, promoting fertility in both genders. Date palm pollen (DPP) has shown gonadal-stimulating potency in females and can significantly increase testosterone levels in individuals with Oligoasthenozoospermia and follicle-stimulating hormone (FSH) levels in patients with Azoospermia, making it as a potential treatment for reproductive health. According to the literature, date fruit is a highly nutritious fruit compared to other commonly consumed fruits. In conclusion dates have been shown to have a significant impact on various female reproductive parameters such as oogenesis, oocyte development, hormone modulation, and pregnancy strength.

Alternative Processing Options for Improving the Proteins Functionality by Maillard Conjugation.

Conjugation of the proteins with carbohydrates, occurring in the early stages of the Maillard reactions, received increased attention because of the high potential to ensure the improvement of the biological activity and functional properties of the proteins of different origins. The Maillard conjugates are conventionally formed through wet or dry heating, but the use of alternative technologies involving ultrasound, microwave, pulsed electric fields, high-pressure, or electrodynamic treatments appears to be efficient in accelerating the reaction steps and limiting the formation of toxic compounds. An overview of the mechanisms of these processing technologies, the main parameters influencing the Maillard conjugate formation, as well as their advantages and disadvantages, is provided in this paper. Different strategies employing these alternative technologies are reported in the literature: as pretreatment of the proteins, either alone or in admixture with the carbohydrates, followed by conventional heating, as a single alternative treatment step, or as a combination of heating and alternative processing. The desired functional properties of the proteins can be achieved by selecting the appropriate processing strategy and optimizing the reaction parameters. Moreover, alternative technologies can be exploited to obtain Maillard conjugates with remarkable biological activity in terms of antioxidant, antimicrobial, antihypertensive, anti-inflammatory, antimutagenic, or bifidogenic properties.

Tannic acid purification from pomegranate peel via tannic acid imprinted particle-embedded cryogel column

Tannic acid (TA) is hydrolysable tannin found in the leaves and bark of many herbaceous and woody plants. Purification of TA is important due to its antibacterial, antihistaminic, antioxidant, antimutagenic and antitussive properties. In this study, 2-hydroxyethyl methacrylate-based TA-imprinted particle embedded cryogel (TA-MIP) was synthesized to purify TA from pomegranate peel. Furthermore, non-imprinted particle embedded cryogel (NIP) was synthesized to determine specific adsorption properties of TA-MIP, and control cryogel was synthesized without embedding procedure. The synthesized cryogel columns were characterized via scanning electron microscopy, Brunauer-Emmett-Teller surface area analysis, fourier-transform infrared spectroscopy, and swelling studies. Particle-embedding procedure resulted in a significantly higher specific surface area of particle-embedded columns (TA-MIP and NIP, 29 m2/g and 25 m2/g, respectively) than the specific surface area of control cryogel (9 m2/g). Adsorption studies were performed from aqueous solutions and maximum TA adsorption was found to be 34.4 mg/g for TA-MIP, 3.9 mg/g for NIP, and 2.8 mg/g for control cryogel. Within the scope of selectivity study, it was demonstrated that the synthesized columns have a high selectivity for TA against gallic acid (GA) and quercetin (QCT). Finally, purification of TA directly from pomegranate peel extract was studied and results were confirmed by HPLC. Furthermore, it has been proven that TA-MIP cryogel columns can be repeatedly used up to ten-times without any remarkable reduction in the TA adsorption amount.

Antioxidant, antimutagenic and antigenotoxic properties of Plectranthus species

Dichlomethane (DCM) and 90% methanol of sixteen Plectranthus species were investigated for antioxidant activity using the DPPH (2, 2-diphenyl-1-picrylhydrazyl), ABTS (2, 2-azinobis (3-ethylbenzothiazoline 6-sulfonate)) and FRAP (ferric reducing antioxidant power) assays. The Plectranthus species were also investigated for antimutagenicity using the Ames assay and antigenotoxicity using the vitotox assay. Dichloromethane extracts of Plectranthus barbatus, P. neochilus and P. argentatus, as well as 90 % methanolic extracts of Plectranthus caninus, P. fruticosus james and P. argentatus had the highest antioxidant activity in the DPPH assay. DCM extracts of P. amboinicus, P. montanus P. neochilus and P. madagascariensis had moderate to strong antimutagenicity in the Ames test at the highest concentration tested. In the vitotox assay, dichloromethane extracts of P. caninus, P. neochilus, P. ernstii, P. verticillatus, P. madagascariensis, P. montanus and the 90 % methanol extract of P. verticillatus were cytotoxic at the highest concentration tested. Of the 32 plant extracts investigated in this study, 68% showed better antioxidant activity than ascorbic acid (vitamin C), particularly in the DPPH and ABTS assays. Additionally, most plant extracts investigated in this study had antimutagenic properties against aflatoxin B1- induced mutagenicity in either the Ames or vitotox assay, or both, without a clear trend or correlation between antioxidant activity and antimutagenic properties.

Overview of Lemna sp. in Aquaculture: Flavonoids for Boosting the Immune System in Fish

This study aims to provide information that the content of flavonoids in Lemna sp. can be an effort to control disease in fish. The research method used is a quantitative descriptive method. The conclusion of the research is to show the ability of Lemna sp. can be used in the prevention of disease in fish. Lemna sp. has immune-modulatory properties, namely the ability to increase phagocytosis so that it can increase the body's immunity in fish. Flavonoid content in Lemna sp. has antioxidant, anti-inflammatory, antimutagenic, and anticarcinogenic properties coupled with their capacity to modulate cellular enzyme function. Flavonoids also have the potential to stimulate the immune system.

Efek konsumsi kurma ajwa (phoenix dactylifera L) terhadap kadar glukosa darah

ABSTRACTBackground: Ajwa dates (Phoenix Dactylifer L.) have many therapeutic potentials, including cell strengthening, anti-mutagenic, antidiabetic, antibacterial, antifungal, antitumor, neuroprotective, and gastroprotective properties. Ajwa dates as an antidiabetic agent, has phytochemical content such as phenolic acids possessed by Ajwa dates can inhibit α-amylase and α-glucosidase so as to reduce blood sugar levels in the body. flavonoids act as antidiabetic agents through their effects as antioxidants by reducing oxidative stress and maintaining liver and kidney function.Objective : To determine the effect of consumption of Ajwa dates (PhoenixDactylifera L.) on blood sugar levels during pregnancy in pregnant women at risk of preeclampsia. Methods : This study uses search databases used in PubMed, Science Direct, and Google Scholar. The keywords used in the initial search for articles were ajwa dates, Phoenix Dactylifera L, Antidiabetic, Blood glucose obtained as many as 3,820 articles. In the end, only 6 articles were used in accordance with the analysis of objectives, topic suitability, inclusion and exclusion criteria, research methods, samples, research ethics, and limitations.Results: The results showed that from all the literature used, the majority showed a decrease when giving ajwa dates to reduce blood glucose levels. Giving ajwa dates can reduce blood glucose levels because the phytochemical content in ajwa dates is very effective in raising blood glucose levels.Conclusion : There is an effect of giving ajwa dates on lowering blood glucose levels. Ajwa dates contain complex carbohydrates, beneficial phytochemicals, sterols, healthy fatty acids, carotene, and flavonoids which have many medicinal activities, one of which is antihyperglycemic or antidiabetic benefits that can reduce blood glucose levels.Keywords: Ajwa Date, Phoenix Dactylifera L, Blood Glucose.

Anticancer and Antimutagenic Properties of Pogonatherum paniceum on Colorectal Cancer Cells.

Pogonatherum paniceum (P. paniceum) (Lam.) Hack. plays an important role in detoxification. However, its anticancer activity has not yet been elucidated. The aim of our study was to examine the suppressive proliferation, anti-migration and mutagenic/antimutagenic properties of P. paniceum. Moreover, we set out to determine the cellular mechanism underlying its antiproliferation. To investigate P. paniceum's anticancer ability, HCT116 and HT29 cell lines were treated with a water extract containing P. paniceum, and then the cell viability was examined using the trypan blue exclusion method which were compared to HEK293 (non-cancerous cells). The anticancer effects were investigated by MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) and colony formation assay. Apoptosis induction, cell cycle distribution, and migration abilities were assessed by cell death detection enzyme-linked immunoassay (ELISA), flow cytometry, and wound healing assay. Finally, the mutagenicity and antimutagenicity were evaluated using the micronucleus assay. Treatment with P. paniceum caused a loss of cell viability in HCT116 and HT29 cells (not found in HEK293), which had an IC50 (half-maximal inhibitory concentration) of 1,156.2 and 1,207.0 µg/mL, respectively. We found that P. paniceum significantly inhibited the proliferative function of HCT116 and HT29 cells. To find the mechanism that exerts a suppressive proliferation effect on P. paniceum, we determined the DNA fragmentation and cell cycle distribution. We also found that P. paniceum treatment increased apoptosis and arrested of the cell cycle at G0/G1 remarkably when compared with the control group. Moreover, P. paniceum could decrease the migration of HCT116 and HT29 cancer cells. Finally, the treatment of P. paniceum did not induce micronucleus formation but did decrease the micronucleus frequency against mutagen-mitomycin C. P. paniceum did not possess any toxicity (cytotoxic and mutagenic) but has the potential for anticancer activity against human colorectal cells by increasing apoptosis, which leads to the suppression of cell proliferation. P. paniceum also inhibits cell migration and exerts antimutagenicity, thereby suggesting that P. paniceum might be useful for colorectal cancer treatment.

Metabolomic approach for phytochemical assessment of Murraya koenigii fruits during different maturity stages

A detailed metabolomic study was performed on various maturation stages of Murraya koenigii fruit pulps, seed, and leaf. Among the fruit pulps, stage 6 had the highest TPC (13.27 mg/g of GAE) and TFC content (6.16 mg/g RE). The extracts also showed promising free radical scavenging activity, especially in the seed (IC50DPPH 427 μg/mL). Metabolomics study revealed the identification of 133 metabolites in fruit pulps, seeds and leaves using the METLIN database. In silico PASS software analysis predicted the antimutagenic property of myricetin and bismurrayaquinone A. Pathway analysis revealed the phenylpropanoid biosynthesis pathway as one of the major pathways present in the fruit pulps. This detailed metabolic report of M. koenigii fruit maturation report brings a new insight into phytochemicals and their distribution in seed, pulps and leaves along with nutritive values and can be considered for nutritive and therapeutic purposes.

Enhancing immunity against carcinogens through probiotics: A literature review

This literature review explores various aspects of using probiotics as a dietary practice to mitigate the effects of toxic compounds. The discussion highlights the importance of considering factors such as timing and composition of probiotic consumption for maximum benefits. Studies have demonstrated the potential of probiotics to inhibit Deoxyribonucleic Acid (DNA) damage and reduce the occurrence of aberrant crypts in animal models when administered before exposure to toxicants. Furthermore, probiotics have been found to metabolize genotoxic compounds into inactive forms, indicating their potential role in detoxification processes. The binding activity of probiotics against toxicants has been widely studied, but there is a need for further research on the metabolites produced during these interactions. Additionally, the presence of other compounds in the food matrix and their competitive effects on probiotic binding should be investigated to understand the full picture. The strain-dependent nature of the probiotic activity and the variability of their antimutagenic properties for different mutagens further highlight the complexity of their functionality. Considering these findings, it is recommended to conduct a careful risk assessment to evaluate the safety of probiotics and their metabolites, taking into account the potential risks and benefits associated with their use. This will help ensure the responsible application of probiotics in food safety and human health initiatives.

Cellular and Molecular Activities of IP6 in Disease Prevention and Therapy

IP6 (phytic acid) is a naturally occurring compound in plant seeds and grains. It is a poly-phosphorylated inositol derivative that has been shown to exhibit many biological activities that accrue benefits in health and diseases (cancer, diabetes, renal lithiasis, cardiovascular diseases, etc.). IP6 has been shown to have several cellular and molecular activities associated with its potential role in disease prevention. These activities include anti-oxidant properties, chelation of metal ions, inhibition of inflammation, modulation of cell signaling pathways, and modulation of the activities of enzymes and hormones that are involved in carbohydrate and lipid metabolism. Studies have shown that IP6 has anti-oxidant properties and can scavenge free radicals known to cause cellular damage and contribute to the development of chronic diseases such as cancers and cardiovascular diseases, as well as diabetes mellitus. It has also been shown to possess anti-inflammatory properties that may modulate immune responses geared towards the prevention of inflammatory conditions. Moreover, IP6 exhibits anti-cancer properties through the induction of cell cycle arrest, promoting apoptosis and inhibiting cancer cell growth. Additionally, it has been shown to have anti-mutagenic properties, which reduce the risk of malignancies by preventing DNA damage and mutations. IP6 has also been reported to have a potential role in bone health. It inhibits bone resorption and promotes bone formation, which may help in the prevention of bone diseases such as osteoporosis. Overall, IP6’s cellular and molecular activities make it a promising candidate for disease prevention. As reported in many studies, its anti-inflammatory, anti-oxidant, and anti-cancer properties support its inclusion as a dietary supplement that may protect against the development of chronic diseases. However, further studies are needed to understand the mechanisms of action of this dynamic molecule and its derivatives and determine the optimal doses and appropriate delivery methods for effective therapeutic use.

Synthesis and Evaluation of Thioamide and Bromo Derivatives of 1,3-Diphenylpropane-1,3-dione as 5α-Reductase Inhibitors.

1,3-Diphenylpropane-1,3-dione (1) is an acetylacetone β-diketone in which both of the methyl groups have been replaced with phenyl groups. It is a component of licorice root extract (Glycyrrhiza glabra) and has anti-mutagenic and anti-cancer properties. It functions as a metabolite, an anti-mutagen, and an anti-neoplastic agent. It is an aromatic ketone and a β-diketone. 1,3-Diphenylpropane-1,3-dione (1) is primarily used in PVC hard and soft materials, including plates, films, profiles, pipes, and fittings. This research aims to examine the utility of 1,3-diphenylpropane-1,3-dione (1) for the synthesis of a variety of new heterocyclic compounds, such as thioamide, thiazolidine, thiophene-2-carbonitrile, phenylthiazole, thiadiazole-2-carboxylate, 1,3,4-thiadiazole derivatives, 2-bromo-1,3-diphenylpropane-1,3-dione, new substituted benzo[1,4]thiazines, phenylquinoxalines, and imidazo[1,2-b][1,2,4]triazole derivatives of potential biological activity Methods: 1,3-Diphenylpropane-1,3-dione (1) was used as a starting compound for the synthesis of 3-oxo-N,3-diphenyl-2-(phenylcarbonyl)propanethioamide (3) and 2-bromo-1,3-diphenylpropane-1,3-dione (25), which can be used for further preparations. The 5α-reductase inhibitor activity of some of the synthesized compounds was also tested in vivo; the ED50 and LD50 data were established, Results: Using IR, 1H-NMR, mass spectroscopy, and elemental analysis, the structures of all produced compounds were elucidated. Some of these prepared compounds were reported as 5α-reductase inhibitors. New heterocyclic compounds can be formed via 1,3-diphenylpropane-1,3-dione (1), and some of these compounds can act as 5α-reductase inhibitors.

A comprehensive review on the medicinal usage of Podocarpus species: Phytochemistry and pharmacology.

Plants of the Podocarpus species belong to the Podocarpaceae family and are largely distributed in the southern hemisphere. Beside the commercially and ecologically valuable, plants of the Podocarpus species are also used in traditional medicines in some countries for treating asthma, fever, venereal diseases, eye diseases, etc. AIM OF THE STUDY: In recent decades, the identities and pharmacological activities of phytochemicals extracted from Podocarpus plants have been widely studied. However, there have been no comprehensive and systematic reviews. This article aims to systematically review the latest research on the putative mechanisms underlying pharmacological actions of phytochemicals from the Podocarpus species, as well as to lay a foundation for promoting the development of plant resources from this genus, further drug research, and product development. A comprehensive search of PubMed, Google Scholar, Web of Science, Elsevier and CNKI databases was conducted using the keywords "Podocarpus", "traditional usage", "phytochemistry", "pharmacology", "nagilactone", etc. Related papers published among July 1964 to February 2023 were collected to summarize the research progress. All plant names were determined through the "The Plant List" (http://www.theplantlist.org/). To date, 262 chemical constituents have been isolated and identified from 26 Podocarpus plants; among these, norditerpene bilactone is the main pharmacologically active component. Norditerpene bilactones are reported to have anti-cancer, anti-inflammatory, antioxidant, antibacterial, anti-tyrosinase, neuroprotective, anti-plasmodial, anti-mutagenic, and anti-atherosclerotic properties as well as other pharmacological activities, which support its traditional uses. Extensive studies on phytochemistry and pharmacology of Podocarpus species lead to discovery of a series of hopeful leading compounds with unique chemical structure, especially the nor- and bis-norditerpenoid dilactones with four isoprene units. These compounds have been proved to possess various pharmacological activities. This review will provide a reference for further research and promote the idea of combining modern research with traditional medicinal applications of Podocarpus plants.