The present study was conducted to determine antimicrobial and radical scavenging activity of Atylosia lineata Wt. & Arn. (Fabaceae). The shade dried and powdered leaves were extracted using methanol. Antibacterial and antifungal activity of extract was determined by agar well diffusion and poisoned food technique respectively. Antiradical efficacy was determined by DPPH and ABTS radical scavenging assays. Total phenolic content of leaf extract was estimated by Folin-Ciocalteau Reagent method. The extract was effective against all test bacteria with marked inhibitory effect against Pseudomonas aeruginosa . Gram negative bacteria were susceptible to higher extent than Gram positive bacteria. The extract inhibited the growth of all test fungi to >50%. Among fungi, Colletotrichum capsici was inhibited to higher extent. The extract scavenged DPPH and ABTS radicals in dose dependent manner. At extract concentration 100µg/ml and higher, >90% scavenging of both radicals was observed. The total phenolic content of leaf extract was found to be 81.36mg GAE/g of extract. The plant appears promising to treat infectious diseases and oxidative damage. K e y w o r d s: A t y losia lineate Agar well diffusion, Poisoned food technique, DPPH, ABTS, Total phenolic