Antidandruff Activity Research Articles

IN VITRO STUDY OF AYURVEDIC CONDITIONER W.S.R. TO ANTIDANDRUFF ACTIVITY

Objective: Dandruff is the unusually high shedding of dead skin cells from the scalp. It is a common scalp disorder affecting almost half of the post pubertal population. In the market many treatment options are available but herbal preparation is the best option to treat dandruff. Thus, in the present research work Ayurvedic conditioner prepared with plant extract (decoction) was studied to evaluate antidandruff activity against Staphyllococus aureus & Candida albicans. Method In vitro study was conducted to evaluate the antidandruff effect of Ayurvedic conditioner against S. aureus & C. albicans. The evaluation done by adopting Kirby-Bauer Agar Well diffusion method. Antibacterial activity was compared with the standard references. Result Study revealed that Ayurvedic conditioner was susceptible for the same microbes in less concentration. Conclusion Ayurvedic conditioner was found to be the most effective formulation against dandruff causing factors; s.aureus and c.albicans.

Formulation and Evaluation of Polyherbal Shampoo to Promote Hair Growth and Provide Antidandruff Action

The assurance of therapy with minimal side effects has been proven that ayurvedic formulation is to be promising for cosmetic use too. Maintenance of other factor shall not fulfill the need so extraneous treatment is essential that to which is safe. In case of hair disorder like dandruff problem, proper selection of ayurvedic ingredient with their required amounts, dosage form can be formulated as powder shampoo to fight against dandruff. A more radical approach in reducing the synthetic ingredient is by incorporating natural extract whose functionality is comparable with their synthetic ingredient. A shampoo is a cleansing aid for the hair and is counted among the foremost beuty products. Several herbs are reported to be effecting is controlling such fungi in our laboratory an antidandruff herbal shampoo –Herbello was prepared and characterized. Certain improvements, especially with respect to viscosity, conditioning ability and antidandruff activity were needed. Most effective herbs were chosen and used in different combination for preparation of shampoo in order to improve antidandruff activity. This herbal shampoo was formulated using natural ingredient like Bhringraj (Eclipta Alba), Fenugreek(methi), Azadirachta indica (neem), Acacia concinna (shikakai), Sapindus mukorossi(reetha), Ocimum santum (tulsi), Piper betle (paan), Hhibiscus rosa sinensis (Gurhal), Camellia sinesis (green tea leaf), Citrus aurantifolia (lemon), Phyllanthus emblica (amla), Eclipta alba (Brhingaraj leaf powder), Aloe Vera (Aloe leaf Powder), Ashwagandha powder, Triphala powder Nagarmotha Powder, Brahmi Root. The formulation at laboratory scale was done and evaluated for number of parameters to ensure its safety, stability and efficacy. Keywords: Hair Dandruff shampoo, cream shampoo, Herbal Material, Antidandruff shampoo.

The Development of Creambath Preparation with Combination of Garlic Extract (Allium sativum L.) and Custard Apple Seeds Extract (Annona squamosa L.) as Anti-Dandruff and Anti Head Lice (Pediculus humanus capitis)

Allicin and ajoene are active compounds in garlic which have proven to be of benefit as antifungal. Meanwhile, the content of oleic acid and triglycerides from the custard apple seeds extract showed anti-fungal activity in vitro. This study aimed to obtain a preparation of creambath combination of garlic extract and custard apple seeds extract which have anti-dandruff and anti head lice activity. Garlic extract was obtained by adding phosphate buffer with freeze-dry method, while custard apple seeds extract was obtained from maceration using petroleum ether. Garlic extract provides an anti-dandruff effect with a minimum inhibitory concentration (MIC) of 6.25 mg/mL and the smallest custard apple seeds extract (LD 100%, 3 hours) which is 3.13 mg/mL can provide anti head lice effect. Variations in the concentration of the combination of garlic extract and custard apple seeds extract used in creambath preparations were (6.25 mg/mL and 3.13 mg/mL), (12.5 mg/mL and 6.25 mg/mL) and (25 mg/mL and 12.5 mg/mL). The resulting creambath preparations have characteristics that are light green, green tea flavored, homogeneous, semisolid form, o/w cream type, pH 5.85-6.25, viscosity 34,000-72,000 cps with thixotropic pseudoplastic flow properties, average size particle 34.31-57.66 μm, anti-dandruff activity with diameters of inhibitory 9, 12, and 16 mm in 72 hours incubation time and LD 100% hair lice activity in 157, 133 and 105 minutes.

Synthesis of palladium nanoparticles via a green route using Lagenaria siceraria: assessment of their innate antidandruff, insecticidal and degradation activities

The safe and green processes development for the nanoparticle synthesis is one of the key factors of nanotechnology concept. The present investigation describes the dye degradation, insecticidal and antidandruff activity of synthesized Palladium nanoparticles (Pd NPs) by utilizing the aqueous extract of Lagenaria siceraria peel which acts as the novel biomass product is reported for the first time. The synthesized Pd NPs were characterized by TEM, UV—visible spectroscopy, FTIR, SEM and XRD investigation. The absorption maxima noticed in the UV–vis show the highest peak close to 362 nm, which is the common characteristics for Pd NPs. FTIR spectra were recorded to recognize the biomolecules associated with the synthesis process, which indicated the absorption bands at 3288.63, 2926.01, 1597.06, 1379.10 cm−1. XRD examination demonstrated that these are very crystalline Pd NPs having a normal size of 21 nm. SEM and TEM images demonstrated the shape and the particles size range of 16–73 nm. The green synthesized Pd NPs showed significant antidandruff activity against Malassezia furfur and Malassezia pachydermatis assayed using agar diffusion assay. Phyto-synthesized Pd NPs exhibited significant insecticidal activity against Sitophilus oryzae. Furthermore, Pd NPs were also used for the remediation of a Bismarck brown. Synthesized Pd NPs exhibited 84% degradation activity of Bismarck brown. Overall, the results of our present study recommends that peel of L. siceraria is a best green source for the eco-friendly synthesis of Pd NPs which further can be utilized as a novel biocidal agent against dandruff causing pathogens, insects and also acts as a strong photocatalytic agent.

To study the antidandruff activity of rosemary oil, basil oil, coleus oil over selenium sulfide

To study the antidandruff activity of rosemary oil, basil oil, coleus oil over selenium sulfide

Evaluation of Anti-Acne and Anti-Dandruff Activity of Seed Protein Extracts

Propionibacterium acnes and Staphylococcus epidermidis have been recognized as pus-forming bacteria triggering an inflammation in acne and Malassezia spp, are the causative agent of dandruff in people who have over active sebaceous glands. The present study was conducted to evaluate antimicrobial activities of seed protein extracts from the seeds like flax seeds, soap nuts and pierre against the acne and dandruff causing organisms. The seed proteins were extracted by homogenizing the seeds powder with phosphate buffer followed by ammonium sulphate precipitation and dialysis for purification of protein. These proteins are used to assess the anti-acne and anti-dandruff activity using well diffusion method. MIC of the effective protein extract is determined.

Basic Phytochemical Study and Evaluation of Antidandruff Potencial of Fruit Juices of Citrus Limon. L

This study investigates the scientific proof for phytochemical composition and also the proof of antidandruff activity. Screening of phytoconstituents was carried out on the juice concentrates using established procedures. The anti-dandruff activity was carried out by Agar Well Diffusion Assay method against Malassezia furfur, which is the organism responsible for dandruff. The result of antidandruff activity revealed that juice concentrates of the fruits of citrus lemon (10 mg extract/mL) exhibited highly significant antifungal activity against Malassezia furfur. The results of Minimum inhibitory concentration (MIC) study reveal that the extract possesses anti-dandruff activity above the concentration of 0.1%w/v. The results of MIC study revealed that the extract possesses anti-dandruff activity in a concentration-dependent manner against the test organism and were comparable to the standard drug.

Discovering the Dandruff Remidial Property of Albizia saman Leaf Extract Towards Malassezia Species

Dandruff a potential threat in the modern day life especially in hair dressing can be overcome by using herbal treatment especially in using with Albizia saman methanolic leaf extract which yielded 34.72 % of the phytoconstituent in the 24 hour incubation. The dandruff causing fungi was isolated using Potato Dextrose agar and a yeast like was observed with a CFU of 48.5 and microbiologically screened using wet mount with Lacto Phenol Cotton Blue staining which showed oval button like appearance and physiological metabolite was detected for the presence of Catalase which also showed positive which confirms the presence of Malasszia ,the dandruff fungi. The methanolic extract of A. saman leaf was evaluated for the antidandruff potential against the dandruff causing fungi Malassezia species using diffusion assay and a MIC detected to be 78.4 mcg .To reveal the antidandruff potential of A. saman leaf extract, the extract was formulated in Oil and Shampoo which can be applied on day usage as Antidandruff agent .The formulated shampoo was evaluated for its detergency which exhibit 53.68 % foam dispersion. To conclude the formulated product – Oil and Shampoo containing A. saman methanolic extract was determined for its antidandruff activity against Malassezia species which exhibited a significant inhibitory zone activity of 19mm each respectively. Further study can be widely expanded to determine the potential substance along with its mechanism which inhibits the fungal growth through in vivo methods.

Evaluation of In Vitro Antidandruff Activity of Biotransformed Material (Extract) of <i>Nicotiana tabacum</i> Leaves

The methods of preparing new derivatives of known substrates were applied to modify organic compounds like Carbohydrates, steroids, sterols, terpenoids, flavonoids, alkaloids, antibiotics, amino acids etc. Such alteration encompasses many changes in the substrate moiety by the addition, degradation or modification. When basic structure of resultant molecule remains unaltered, the microbial biotransformation can be applied for formation of desired derivatives of precursor. <strong>Aim:</strong> To determine the<em> in-vitro</em> anti-dandruff activity of biotransformed material of <em>Nicotiana tabacum</em> leaves. <strong>Material and Method:</strong> The collected leaves about 200 g were macerated in cow urine (1 L) for time period of 28 days. After the completion of this time period, the biotransformed material was harvested, lyophilized and powdered (20 g). Regular aqueous extract of the leaves was also prepared for comparative study. Various concentrations, such as 12.5, 25, 50, 100, 500 mg/ml, of aq. extract and biotransformed material of Nicotiana tabacum leaves were prepared and tested for <em>in vitro</em> anti dandruff activity using strain of <em>Malassezia</em> (Pityrosporum) <em>furfur</em> and compared with marketed ayurvedic, homoepathic and allopathic (Ketoconazole 2 %) formulations.<strong> Result:</strong> The regular extract and the biotransformed material were screened for phytochemicals. It showed the presence of alkaloids, prominently. The strains responsible for the biotransformation process were also evaluated and identified as <em>Bacillus licheniformis</em> and <em>Bacillus megaterium</em>. The biotransformed material of <em>Nicotiana tabacum</em> leaves shows promising effect against <em>Malassezia</em> (Pityrosporum) <em>furfur</em>, in terms of zone of inhibition of about 7 mm at 100 mg/ml 26 mm at 500 mg /ml, respectively. The results were found promising as compared to the regular aqueous extract, marketed formulations including Ketoconazole 2 % preparation. <strong>Conclusion</strong>: The study shows the improved potential of the cow urine biotransformed material over the normal extract of the same plant. It was evident that the dandruff growth was significantly inhibited by treatment with biotransformed material. Hence, <em>in vitro</em> studies of microbial biotransformed <em>Nicotiana tabacum</em> leaves was found to have a potential anti-dandruff activity between 100 mg/ml to 500 mg/ml, compared with its aqueous extract, marketed ayurvedic, homoepathic and allopathic formulations.

Comparison of potency of antifungal action of dandruff shampoos and different plant extracts

Context: Dandruff a very common scalp disorder with high prevalence in population is caused by numerous host factors in conjunction with Malassezia furfur. Most of the commercially available anti-dandruff hair shampoos contain some form of antifungal agent(s) that appear to reduce the incidence of the disease. There are no good scientific studies done to prove the antifungal activity of commercially available hair shampoos. Aim: In this study commercially available shampoos were assessed for antifungal activity against a human dandruff isolate of M. furfur. The shampoos were Head & Shoulders, Clinic All Clear, and Pantene etc. The results demonstrated that all six of the assayed hair shampoos have some antifungal effect on growth of M. furfur. These products have poor efficacies, more side effects and give scope for recurrence of symptoms. Methods and Materials: Therefore different plant extracts that possess various active compounds which have antifungal activity could help to overcome the incidence of the disease and also avoid the emergence of resistance in the pathogen. The plant extracts were tested in different concentrations like 1:5, 1:10, 1:20 and they were hibiscus, neem, soap nut, etc. The inhibitory action was studied using agar well assay and disc diffusion method and the results indicated in percentage of inhibition. Conclusion: The study was significant as not only efficient known plant products with anti-dandruff activity could be compared with commercially available shampoos but also their better efficacies at minimum concentrations could be identified. This can help make a polyherbal mixture that could be incorporated in hair oil or shampoos for better anti-dandruff activity.

QUANTITATIVE MICROSCOPY AND IN VITRO ANTI DANDRUFF ACTIVITY OF DICHROSTACHYS CINEREA WIGHT AND ARN

Natural products have been used in traditional medicine for thousands of years and recently have been of increasing interest, since the cost are usually lower and they are considered less toxic by the public. The plant Dichrostachys cinerea is profusely branched thorny shrub distributed throughout the dry and warm parts of India belonging to the family Mimosaceae. The standardization of the leaves of D. cinerea were performed by Quantitative microscopy such as stomatal no, stomatal index, vein islet no. and vein termination no and foam index were examined. The aim of our study was to assess in vitro antidandruff activity of ehanolic extract of leaves of D. cinerea by disc diffusion method, the zone of inhibition were measured. It was observed that, no profound activity at lower concentration (50 mg/ml, 100 mg/ml) and showed only moderate activity at higher concentration (250 mg/ml and 500 mg/ml) against the fungi Mallazessia furfur or Pityrosporum ovale.

Eczémas de contact atypiques ou sévères à l’huile essentielle de cannelle

The intent of the present investigation was to explore the utility of alcoholic and aqueous extract of Punica granatum L. as hair growth promoter along with anti-lice and antidandruff activity. A filter paper diffusion approach was employed for screening of the pediculocidal and ovicidal activity. Albino mice, preselected for their telogen phase of hair growth were used during the study. The prepared extracts, Minoxidil and control were applied over shaved skin surface on to the backs of mice to assess telogen to anagen transition. The qualitative and quantitative analysis was performed. The outcome of the studies revealed that Punica granatum L. alcoholic and aqueous extracts exhibited prominent anti-lice activity. The transition of telogen to anagen phase of the number of anagen hair follicle was observed in approximately 45, 27 and 51% of animals treated with alcoholic and aqueous extract of Punica granatum L., and Minoxidil, respectively, which suggest the hair growth promoting potential of the extract of Punica granatum L. Also, 3 % Punica granatum L. alcoholic extracts exhibited a potent antidandruff activity against fungal strains tested. Maltol, was identified as a principal phytoconstituent in the alcoholic extract. The findings greatly suggest anti-lice, antidandruff and hair growth promoting potential of the extract of Punica granatum L.

Dano: A herbal solution for dandruff

Dano, a poly-herbal hair oil was studied for anti-dandruff activity using microbiological and clinical tests. There was a clear symptomatic relief from dandruff in all the volunteers after 10 days of use. Further, the isolation of Pityrosporum ovale, the causative organism of the dandruff in culture was not possible after use of the Dano oil. The plant extracts is from Wrightia tinctoria (Indrajev), Cassia alata (Dadmari) and bitter fraction of Azadirachta indica (Neem or Nimba). Methylene blue reductase test was employed to study the anti-dandruff efficacy of the oil

Enhancement of antidandruff activity of shampoo by biosynthesized silver nanoparticles from Solanum trilobatum plant leaf

The present investigation describes simple and effective method for synthesis of silver nanoparticles via green route. Solanum trilobatum Linn extract were prepared by both conventional and homogenization method. We optimized the production of silver nanoparticles under sunlight, microwave and room temperature. The best results were obtained with sunlight irradiation, exhibiting 15–20 nm silver nanoparticles having cubic and hexagonal shape. Biosynthesized nanoparticles were highly toxic to various bacterial strains tested. In this study we report antibacterial activity against various Gram negative (Klebsiella pneumoniae, Vibrio cholerae and Salmonella typhi) and Gram positive (Staphylococcus aureus, Bacillus cereus and Micrococcus luteus) bacterial strains. Screening was also performed for any antifungal properties of the nanoparticles against human pathogenic fungal strains (Candida albicans and Candida parapsilosis). We also demonstrated that these nanoparticles when mixed with shampoo enhance the anti-dandruff effect against dandruff causing fungal pathogens (Pityrosporum ovale and Pityrosporum folliculitis). The present study showed a simple, rapid and economical route to synthesize silver nanoparticles and their applications hence has a great potential in biomedical field.

Studies on the antidandruff activity of the essential oil of coleus amboinicus and eucalyptus globulus

ObjectiveTo determine the effect of the essential oil of Coleus amboinicus and Eucalyptus globules as potential antidandruff agent. MethodsFresh leaves of Coleus amboinicus and Eucalyptus globules were collected from the Horticultural Research Station, Yercaud, Salem, Tamil Nadu, India. The collected leaves were washed thoroughly and shade dried for 7-10 days. The essential oils from both the dried plant leaves were obtained by hydro distillation procedure. The chemical compositions of hydro distilled essential oils were analyzed by Gas Chromatography-Mass spectrometry (GC-MS). The pure essential oils and the essential oils diluted with coconut oil of the two plants were used to check antidandruff activity against the dandruff causing fungus Malassezia furfur by agar well diffusion assay. ResultsThe average yield of essential oil from Coleus amboinicus (0.89%) and Eucalyptus globules (0.77%). The results of GC-MS revealed that the presence of eight compounds from Coleus amboinicus essential oil and five constituents from Eucalyptus globules representing 90.45% and 96.32% of the total oil respectively. The major component was identified as Thymol (phenolic rich compocnd) (41.3%) in Coleus amboinicus and in Eucalyptus globules the major constituents was 1-8, Cineol (91.61%). In the antidandruff activity the broad range of inhibitory zone was observed in Eucalyptus globules (37mm) oil compare to Coleus amboinicus (31mm). Ketaconazole based shampoo used as a standard. ConclusionThis study would direct to the establishment of a natural compound from Coleus amboinicus and Eucalyptus globules as an antidandruff agent which can be used for the production of potential antifungal drug and novel pharmaceutical and cosmeceutical leads.

Evaluation of antidandruff activity using polyherbal oil from six medicinal plants

Evaluation of antidandruff activity using polyherbal oil from six medicinal plants

Formulation and evaluation of herbal anti-dandruff shampoo

The petroleum ether extract of Tridax procumbens was found to be effective against dandruff at minimum inhibitory concentration of 10000mg/ml, which was compared with standard Ketoconazole (10mg/ml). Eclipta alba does not show any effect against Ptyrosporum ovale. Formulation (liquid cream shampoo) was developed using active extract of Tridax procumbens and was evaluated using various parameters, which proves its efficacy and safety. The toxicity studies of formulation did not show any toxic effect after 24 hours of application. Keywords: Tridax procumbens, Eclipta alba, Pityrosporum ovale, anti-dandruff activity Nigerian Journal of Natural Products and Medicine Vol. 9 2005: 55-60

Flow injection analysis of zinc pyrithione in hair care products on a cobalt phthalocyanine modified screen-printed carbon electrode

Zinc pyrithione (ZPT) is an antibacterial and antifungal reagent that is often utilized for the antidandruff activity in hair-care shampoos with a composition level up to 1% in the formulation. It has some adverse effects to human and animal if consumed orally. A disposable type of cobalt phthalocyanide modified screen-printed carbon electrode (CoPc/SPE) in couple with flow injection analysis (FIA) was developed for easy and selective analysis of ZPT in commercial hair-care products. Under the optimized FIA conditions, the CoPc/SPE yielded a linear calibration plot in the window of 6–576 μM with sensitivity and detection limit of 1.65 nA μM −1 and 0.9 μM (i.e. 1.42 pg in 5 μl sample loop), respectively, in 0.1 M KOH solution at an applied potential of 0.3 V versus Ag/AgCl. Since the approach is simple, easy, selective, and inexpensive, it offers a potential application of daily ZPT analysis in hair-care products.