Antiallergic Agent Research Articles

Evaluation of the high affinity IgE receptor alpha-IgG1 Fc fusion protein (FCER1A-Fc) as a topical ocular anti-allergic agent

Evaluation of the high affinity IgE receptor alpha-IgG1 Fc fusion protein (FCER1A-Fc) as a topical ocular anti-allergic agent

Designing, docking and molecular dynamics simulation studies of novel cloperastine analogues as anti-allergic agents: homology modeling and active site prediction for the human histamine H1 receptor.

The present study predicts a three-dimensional model for the histamine H1 receptor and the design of antihistamine inhibitors using cloperastine as the core molecule by docking studies. In this work, we predicted a three-dimensional structure of the histamine H1 receptor using the MODELLER9V7 software. The protein structure was developed based on the crystal structure of the histamine H1 receptor, the lysozyme chimera of Escherichia virus T4 (PDB ID: 3RZE_A) target collected from the PDB data bank. Using molecular dynamics simulation methods, the final predicted structure is obtained and further analyzed by VERIFY3D and PROCHECK programs, confirming that the final model is reliable. The drug derivatives of cloperastine were designed and docking was performed with the designed ligands along with the drug. The predicted model of the histamine H1 receptor structure is stable and confirms that it is a reliable structure for docking studies. The results indicate that MET 183, THR 184 and ILE 187 in the histamine H1 receptor are important determinant residues for binding as they have strong hydrogen bonding with cloperastine derivatives. The drug derivatives were docked to the histamine H1 receptor protein by hydrogen bonding interactions and these interactions played an important role in the binding studies. The molecule 1-{2-[(4-chlorophenyl) (phenyl) methoxy] ethyl}-4-methylenepiperidine showed the best docking results with the histamine H1 receptor. The docking results predicted the best compounds, which may act as better drugs than cloperastine and in the future, these may be developed for anti-allergy therapy.

Study of Panchashirishadi Agad on the basis of physicochemical and phytochemical analysis

Background: Many antidotes are mentioned in the classical text for the management of animal poisoning. Panchashirishadi Agad is one of the formulations indicated for Kita Visha (insect bite). Aim: To assess the physicochemical and phytochemical properties of Panchashirishadi Agad.Setting and Design: This was an original analytical study. Materials and Methods: Five parts of Shirish (Albizia lebbeck) were collected from local area of Wardha (Sewagram) and Panchashirishadi Agad was formulated as per the reference of classical text. Result: After the analytical study, the physicochemical properties that include loss on drying at 105°C, total ash value, acid-insoluble ash, water-soluble extractive, alcohol-soluble extractive, pH, and particle size were analyzed and the obtained values were 12.0%, 10.0%, 1.0%, 11.0%, 13.0%, 5.45%, and 100 mesh, respectively. In the similar way, the herbal formulation is devoid of microbial contamination. Phytochemical analysis includes the presence of 48% polyphenols. Conclusion: As the formulation contains more percentage of polyphenols, it can be anticipated that it can act as antiallergic agent.

Synthesis and structure-activity relationships of novel 5-(hydroxamic acid)methyl oxazolidinone derivatives as 5-lipoxygenase inhibitors

Oxazolidinone hydroxamic acid derivatives were synthesised and evaluated for inhibitory activity against leukotriene (LT) biosynthesis in three in vitro cell-based test systems and on direct inhibition of recombinant human 5-lipoxygenase (5-LO). Thirteen of the 19 compounds synthesised were considered active ((50% inhibitory concentration (IC50) ≤ 10 µM in two or more test systems)). Increasing alkyl chain length on the hydroxamic acid moiety enhanced activity and morpholinyl-containing derivatives were more active than N-acetyl-piperizinyl derivatives. The IC50 values in cell-based assay systems were comparable to those obtained by direct inhibition of 5-LO activity, confirming that the compounds are direct inhibitors of 5-LO. Particularly, compounds PH-249 and PH-251 had outstanding potencies (IC50 < 1 µM), comparable to that of the prototype 5-LO inhibitor, zileuton. Pronounced in vivo activity was demonstrated in zymosan-induced peritonitis in mice. These novel oxazolidinone hydroxamic acid derivatives are, therefore, potent 5-LO inhibitors with potential application as anti-allergic and anti-inflammatory agents.

Advances in the chemical and biological diversity of heterocyclic systems incorporating pyrimido[1,6-a]pyrimidine and pyrimido[1,6-c]pyrimidine scaffolds.

Heterocycles incorporating a pyrimidopyrimidine scaffold have aroused great interest from researchers in the field of medical chemistry because of their privileged biological activities; they are used as anti-bacterial, antiviral, anti-tumor, anti-allergic, antihypertensive, anticancer, and hepatoprotective agents. Therefore, the present study aims to investigate the chemistry of heterocycles incorporating pyrimido[1,6-a]pyrimidine and pyrimido[1,6-c]pyrimidine skeletons and their biological characteristics. The main sections discuss (1) the synthetic routes to obtain substituted pyrimidopyrimidines, pyrimido[1,6-a]pyrimidin-diones, pyrimidoquinazolines, tricyclic, tetracyclic, and binary systems; (2) the reactivity of the substituents attached to the pyrimidopyrimidine skeleton, including thione and amide groups, nucleophilic substitutions, condensations, ring transformations, and coordination chemistry; (3) compounds of this class of heterocycles containing a significant characteristic scaffold and possessing a wide range of biological characteristics.

A Review on Therapeutic Potentials of Crocetin-A Carotenoid Derived from Saffron

Natural dyes recognized as carotenoids have found application in health care system due to their wide biological activities, high safety margins and lower cost. Crocetin, a carotenoid constituent of saffron has shown wide range of pharmacological applications due to its significant antioxidant properties. It is used as antifungal, antimicrobial and antiallergic agent. Crocetin has shown significant potential as an anti- tumor agent in animal models and cell culture systems. Current review has shown crocetin’s multispectrum pharmacological benefits for the treatment of various chronic diseases such as cancer, diabetes, parkinson’s and alzheimer’s disease. This review focus on various studies done on crocetin explaining its broa d spectrum pharmacological activities. In addition, this review will also focus on the pharmacokinetic studies of crocetin on human and animals. Crocetin is a highly potent molecule because of its strong antioxidant properties. In the near future, increasing its bioavailability using novel drug delivery methods having minimum side effects will get this promising natural molecule to the forefront of therapy for the treatment of various chronic human diseases like cancer, diabetes, parkinson’s and alzheimer’s disease.

The Putatively Specific Synthetic REV-ERB Agonist SR9009 Inhibits IgE- and IL-33-Mediated Mast Cell Activation Independently of the Circadian Clock.

The cell-autonomous circadian clock regulates IgE- and IL-33-mediated mast cell activation, both of which are key events in the development of allergic diseases. Accordingly, clock modifiers could be used to treat allergic diseases, as well as many other circadian-related diseases, such as sleep and metabolic disorders. The nuclear receptors REV-ERB-α and -β (REV-ERBs) are crucial components of the circadian clockwork. Efforts to pharmacologically target REV-ERBs using putatively specific synthetic agonists, particularly SR9009, have yielded beneficial effects on sleep and metabolism. Here, we sought to determine whether REV-ERBs are functional in the circadian clockwork in mast cells and, if so, whether SR9009 affects IgE- and IL-33-mediated mast cell activation. Bone marrow-derived mast cells (BMMCs) obtained from wild-type mice expressed REV-ERBs, and SR9009 or other synthetic REV-ERBs agonists affected the mast cell clockwork. SR9009 inhibited IgE- and IL-33-mediated mast cell activation in wild-type BMMCs in association with inhibition of Gab2/PI3K and NF-κB activation. Unexpectedly, these suppressive effects of SR9009 were observed in BMMCs following mutation of the core circadian gene Clock. These findings suggest that SR9009 inhibits IgE- and IL-33-mediated mast cell activation independently of the functional circadian clock activity. Thus, SR9009 or other synthetic REV-ERB agonists may have potential for anti-allergic agents.

Phyto-chemical screening of Root Extracts of Glycyrrhiza glabra by Spectroscopic Methods (UV-VIS Spectrophotometer, FTIR and HPLC)

Glycyrrhiza glabra, commonly known as licorice, has traditionally been used in various medicinal preparations as expectorant, antimicrobial, antiviral, anticancer, anti allergic agent. It has also prominent role in skin whitening and has anti-inflammatory and esterogenic properties. This study aims to the characterization of the bioactive constituent glabridin in plant extract of Glycyrrhiza glabra in ethanol, methanol and ethyl acetate using UV-VIS and FTIR and HPLC. A wavelength scan (200-600 nm) by UV-Vis spectrophotometer was performed and the characteristic peaks were recorded. The scan confirmed presence of glabridin with λmax at 281 nm with a small number of additional phytochemicals indicated by the extra peaks. FTIR spectrum of 62% pure glabridin was compared to the spectra shown by the three extracts. Presence of glabridin in all the three extracts was corroborated by HPLC analysis with the retention time of 4.05 minute. Glabridin was also evaluated for its antibacterial activity against Propionibacterium acne, causative agent of Acne vulgaris and found to have the detrimental effect at concentration 500, 1000 and 1500 ppm.

Comparison of cytotoxicities and anti-allergic effects of topical ocular dual-action anti-allergic agents

BackgroundTo investigate the cytotoxicities of the topical ocular dual-action anti-allergic agents (alcaftadine 0.25%, bepotastine besilate 1.5%, and olopatadine HCL 0.1%) on human corneal epithelial cells (HCECs) and their anti-allergic effects on cultured conjunctival epithelial cells.MethodsA Methylthiazolyltetrazolium(MTT)-based calorimetric assay was used to assess cytotoxicities using HCECs at concentrations of 10, 20 or 30% for exposure durations of 30 min, 1 h, 2 h, 12 h or 24 h. Cellular morphologies were evaluated by inverted phase-contrast and electron microscopy. Wound widths were measured 2 h, 18 h, or 24 h after confluent HCECs monolayers were scratched. Realtime PCR was used to quantify anti-allergic effects on cultured human conjunctival cells, in which allergic reactions were induced by treating them with Aspergillus antigen.ResultsCell viabilities decreased in time- and concentration-dependent manners. Cells were detached from dishes and showed microvilli loss, cytoplasmic vacuoles, and nuclear condensation when exposed to antiallergic agents; alcaftadine was found to be least cytotoxic. Alcaftadine treated HCECs monolayers showed the best wound healing followed by bepotastine and olopatadine (p < 0.0001). All agents significantly reduced the gene expressions of allergic cytokines (IL-5, IL-25, eotaxin, thymus and activation-regulated chemokine, and thymic stromal lymphopoietin) and alcaftadine had the greatest effect (p < 0.0001 in all cases).ConclusionsAlcaftadine seems to have less side effects and better therapeutic effects than the other two anti-allergic agents tested. It may be more beneficial to use less toxic agents for patients with ocular surface risk factors or presumed symptoms of toxicity.

Docosahexaenoyl ethanolamide mitigates IgE-mediated allergic reactions by inhibiting mast cell degranulation and regulating allergy-related immune cells

Docosahexaenoic acid (DHA) is a long-chain polyunsaturated fatty acid mainly found in fish oil. Although several studies have suggested that it can alleviate allergy symptoms, its mechanism of action remains to be elucidated. In the present study, we found that docosahexaenoyl ethanolamide (DHEA), a metabolite of DHA produced in the human body, exerts the anti-allergic activity in vitro and in vivo. DHEA suppressed degranulation of rat basophilic leukemia RBL-2H3 cells and bone marrow-derived mast cells in a dose-dependent manner without cytotoxicity. This occurred due to a decrease in Ca2+ influx, which is critical for mast cell degranulation. DHEA also suppressed IgE-mediated passive cutaneous anaphylaxis reaction in mice. In addition, DHEA was demonstrated to lessen an allergic symptom in a mouse model of pollinosis and to alter the production of IgE and cytokines secreted by splenocytes collected from the pollinosis mice. Taken together, this study indicates that DHEA is a promising anti-allergic agent as it inhibits mast cell degranulation and modulates other immune cells.

Design, synthesis and biological evaluation of novel desloratadine derivatives with anti-inflammatory and H1 antagonize activities

To improve the anti-inflammatory activity of desloratadine, we designed and synthesized a series of novel desloratadine derivatives. All compounds were evaluated for their anti-inflammatory and H1 antagonistic activities. Among them, compound 2c showed the strongest H1 antagonistic and anti-inflammatory activity. It also exhibited promising pharmacokinetic profiles and low toxicity. All these results suggest that compound 2c as a novel anti-allergic agent is worthy of further investigation.

Structural and clinical impact of anti-allergy agents: An overview.

Allergic disorders are markedly rising in industrialized countries. The identification of compounds that trigger the immunoglobulin E (IgE)-dependent allergic reaction remain the means to improve the quality of life by limiting patient's exposure to critical allergens. Information concerning the treatment and onset of allergic disorders including atopic dermatitis, allergic rhinitis, and bronchial asthma has been provided by the research over the past decade. Recent studies also indicated that allergic inflammation is associated closely with their exacerbation and progression and indeed is the basic pathophysiology of allergic diseases. As a result of immunological and molecular biological studies our understanding of the mechanism of allergic inflammation with regard to therapeutic agents has improved. While much effort has been paid to developing a new anti-allergic agent, the allergic disease has yet to be completely conquered. The more extensive research will allow the development of new therapeutics to combat allergic diseases. Currently, with respect to mechanism of action anti-allergy drugs are classified into five types including histamine H1 antagonists, leukotriene antagonists, Th2 cytokine inhibitors, thromboxane A2 inhibitors and mediator-release inhibitors. The use of two or more anti-allergy agents together is not acknowledged at present, but this will be the subject of research in the future because with different mechanisms of action anti-allergy agents used at the same time will theoretically increase their effects. This review article focuses on anti-allergy agents highlighting their applications, clinical trials and recent advancement on drugs.

Lamiaceae: An Insight on Their Anti-Allergic Potential and Its Mechanisms of Action.

The prevalence of allergic diseases such as asthma, allergic rhinitis, food allergy and atopic dermatitis has increased dramatically in recent decades. Conventional therapies for allergy can induce undesirable effects and hence patients tend to seek alternative therapies like natural compounds. Considering the fact above, there is an urgency to discover potential medicinal plants as future candidates in the development of novel anti-allergic therapeutic agents. The Lamiaceae family, or mint family, is a diverse plant family which encompasses more than 7,000 species and with a cosmopolitan distribution. A number of species from this family has been widely employed as ethnomedicine against allergic inflammatory skin diseases and allergic asthma in traditional practices. Phytochemical analysis of the Lamiaceae family has reported the presence of flavonoids, flavones, flavanones, flavonoid glycosides, monoterpenes, diterpenes, triterpenoids, essential oil and fatty acids. Numerous investigations have highlighted the anti-allergic activities of Lamiaceae species with their active principles and crude extracts. Henceforth, this review has the ultimate aim of compiling the up-to-date (2018) findings of published scientific information about the anti-allergic activities of Lamiaceae species. In addition, the botanical features, medicinal uses, chemical constituents and toxicological studies of Lamiaceae species were also documented. The method employed for data collection in this review was mainly the exploration of the PubMed, Ovid and Scopus databases. Additional research studies were obtained from the reference lists of retrieved articles. This comprehensive summarization serves as a useful resource for a better understanding of Lamiaceae species. The anti-allergic mechanisms related to Lamiaceae species are also reviewed extensively which aids in future exploration of the anti-allergic potential of Lamiaceae species.

THERAPEUTIC VALUE OF ARCTIUM LAPPA LINN. – A REVIEW

Arctium lappa (AL) (Burdock) has been used for centuries to treat a variety of ailments. Extracts of burdock root (BR) are found in a variety of herbal preparations, as well as homeopathic remedies. Arctium has been used as medicine and health supplement for hundreds of years in Europe, North America, and Asia. It is popular as a medicinal plant in traditional Chinese medicine where it is extensively used for various ailments. In recent years, immense phytochemical and pharmacological screening of Arctium has shown tremendous activity against various diseases. The plant has shown biological activity as anti-inflammatory, antiallergic, antidiabetic, antineoplastic, antimutagenic, antiviral, urolytic, antitoxic, antileukemic, hepatoprotective, growth stimulating, mild laxative, mild diuretic, depurative, diaphoretic, antibacterial, antirheumatic, and antipyretic agent. The present review highlights botanical description, distribution, chemical constituents, and the pharmacological effects of AL. The aim of this review is to correlate active principles of the plant to their pharmacological activity and thus explore the potential of AL as a source of therapeutic agents.

A clean and green approach for odd chain fatty acids production in Rhodococcus sp. YHY01 by medium engineering

Odd chain fatty acids serve as anti-allergic, anti-inflammatory, and antifungal agents, and are useful for the production of biodiesel. Rhodococcus sp. YHY01 utilizes a wide range of carbon sources and accumulate lipids i.e. fructose (37% w/w dcw) glucose (56% w/w dcw), glycerol (50% w/w dcw), acetate (42% w/w dcw), butyrate (65% w/w dcw), lactate (56% w/w dcw), and propionate (62% w/w dcw). In this study, propionate was proved as the best carbon source and produced 69% odd chain fatty acids of total fatty acids, followed by glycerol (13% odd chain fatty acids of total fatty acids). A synthetic medium optimized with response surface design containing glycerol, propionate, and ammonium chloride (0.32%:0.76%:0.040% w/v) facilitated the production of total fatty acids 69% w/w of dcw, and odd chain fatty acids comprised 85% w/w of total fatty acids. Major odd chain fatty acids were in the order C17:0 > C15:0 > Cis-10-C17:1 > 10Me-C17:0 > C19:0 > Cis-10-C19:1.

Clinical observation of the effect of intranasal corticosteroid plaster in the treatment of allergic rhinoconjunctivitis

Objective To investigate the therapeutic effect of short-term application of intranasal corticosteroid ointment in the treatment of allergic rhinoconjunctivitis (ARC). Methods From December 2016 to December 2017, one hundred and sixty-four patients with ARC were diagnosed in the ophthalmological clinic of the Second Affiliated Hospital of Zhengzhou University, and they were divided into observation group (83 cases) and control group(81 cases) according to the digital table.The observation group was treated with tobramycin dexamethasone ophthalmic ointment, and the control group was treated with azzolastine hydrochloride eye drops.The scores of ocular, nasal symptoms and signs were recorded and compared with before treatment, and the clinical effective rates of the two groups were compared. Results After 14 days of treatment, the scores of nasal symptoms and signs in the two groups were significantly lower than those before treatment(all P<0.05). In the observation group, the scores of nasal symptoms and signs after 14 days of treatment were lower than those in the control group, and the differences were statistically significant (t=3.048, P=0.008; t=3.098, P=0.028). The total effective rates of the observation group and control group were 79.51% and 48.19%, respectively, the difference was statistically significant (χ2=18.644, P<0.05). Conclusion The short-term application of intranasal corticosteroid ointment can effectively relieve the clinical symptoms of ARC.The therapeutics is simple and effective, and it is safe and economical.It is very suitable for wide application and popularization in primary clinical hospitals. Key words: Rhinoconjunctivitis; allergic; Allergic conjunctivitis; Allergic rhinitis; Corticosteroid; H1 antihistamines; Intranasal corticosteroid; Drug therapy; Anti-allergic agents; Ointment

Suplatast tosilate reduces radiation-induced lung injury in mice through suppression of oxidative stress

PurposeAlthough radiotherapy is important in the treatment of malignant thoracic tumors, it has harmful effects on healthy tissues. We previously showed that suplatast tosilate, an anti-allergic agent, scavenged reactive oxygen species (ROS), including hydroxyl radicals. Because ROS-mediated oxidative stress is involved in radiation-induced lung injury, we hypothesized that suplatast tosilate could reduce radiation-induced lung injury via suppression of oxidative stress. Methods and materialsMurine alveolar epithelial cells were irradiated with or without a medium containing suplatast tosilate in vitro to determine whether the agent had cytoprotective effects against radiation-induced injury. On the other hand, the thoracic region of C57BL/6 mice was exposed to a single irradiation dose of 15 Gy and the effects of suplatast tosilate were determined by a histological evaluation and assessment of the following parameters: cell number and inflammatory cytokine levels in bronchoalveolar lavage fluid, and oxidative stress markers and hydroxyproline content in pulmonary tissues. ResultsSuplatast tosilate protected murine alveolar epithelial cells in vitro from irradiation-induced inhibition of cell proliferation, which was accompanied by the suppression of intracellular ROS and DNA double-strand breaks induced by irradiation. Oxidative stress markers and the levels of inflammatory and fibrogenic cytokines were upregulated in irradiated murine lungs in vivo. Suplatast tosilate suppressed both oxidative stress markers and the levels of cytokines, which resulted in reduced pulmonary fibrosis and clearly improved the survival rate after irradiation. ConclusionsThese findings demonstrate that suplatast tosilate could be a useful lung-protective agent that acts via suppression of oxidative stress associated with thoracic radiotherapy.

Suppressive effects of type I angiotensin receptor antagonists, candesartan and irbesartan on allergic asthma

The effects of candesartan and irbesartan, antagonists of the type I angiotensin II receptor, were investigated on allergic asthma. The antigen-induced degranulation was measured by evaluating β-hexosaminidase activity in vitro. Additionally, a murine ovalbumin-induced allergic asthma model was used to test the in vivo efficacy. It was observed that while candesartan inhibited the antigen-induced degranulation in rat RBL-2H3 mast cells, irbesartan did not. Administration of candesartan and irbesartan decreased the number of immune cells in the bronchoalveolar lavage fluid and reduced the expression of Th2 (IL-4, IL-5, and IL-13) and Th1 cytokines (IL-2 and IFN-γ) in the lung tissues of mice with ovalbumin-induced allergic asthma. Histological studies revealed that both antagonists reduced inflammation and mucin production in the lungs. Therefore, these findings provide evidence that candesartan and irbesartan could have potential applications as anti-allergic agents.

Traditional Tibetan medicine Anzhijinhua San attenuates ovalbumin-induced diarrhea by regulating the serotonin signaling system in mice

Ethnopharmacological relevanceTibetan medicine has been practiced for 3800 years. Anzhijinhua San (AZJHS), which is a traditional Tibetan medicine, has been effective in the treatment of indigestion, anorexia and cold diarrhea. However, the effects of AZJHS on allergic diarrhea have not been reported. Aim of the studyThe aim of the present study was to elucidate the effect of AZJHS on experimental ovalbumin-induced diarrhea and elucidate its possible mechanism. Materials and methodsFemale BALB/c mice were sensitized by intraperitoneal injection with 50 μg ovalbumin (OVA) and 1 mg alum in saline twice during a 2-week period. From day 28, mice were orally challenged with OVA (50 mg) every other day for a total of ten times. AZJHS (46.8 and 468.0 mg/kg) was orally administered every other day from day 0–46. Food allergy symptoms were evaluated. OVA- specific IgE, 5-HT and its metabolites in serum were determined. Immunohistochemical and histopathology were performed in gastrointestinal tract tissues. 5-HT-related gene expression was assayed in the colon. ResultsSevere symptoms of allergic diarrhea were observed in the model group (diarrhea, anaphylactic response, and rectal temperature). AZJHS (46.8 and 468.0 mg/kg) significantly reduced mouse diarrhea and significantly prevented the increases in OVA-specific IgE levels (P < 0.05), which challenge with OVA. AZJHS (46.8 and 468.0 mg/kg) significantly prevented the increases in 5-HT-positive cells. The nuclei of EC cells in the AZJHS (46.8 and 468.0 mg/kg) group increased in size and the secretory granules were fewer in number compared with those in the model group. AZJHS (46.8 and 468.0 mg/kg) significantly increased the relative fold changes of 5-HTP and 5-HT compared with the model group. The mRNA expression of the serotonin transporter (Sert) and serotonin receptor 3A (Htr3a) was significantly decreased after the 10th challenge with OVA, and AZJHS (46.8 and 468.0 mg/kg) significantly increased these levels. ConclusionsWe demonstrated that the administration of AZJHS attenuated OVA-induced diarrhea by regulating the serotonin pathway. These results indicated that AZJHS may be a potential candidate as an anti-allergic diarrhea agent.

Clinical analysis of eosinophilic gastroenteritis in children

Objective To discuss the clinical manifestations, laboratory indexes, imageological examination results, endoscopic and histopathological features of eosinophilic gastroenteritis(EG) in children. Methods Thirty-three children with EG were collected with clinical manifestations, allergic history, laboratory examination, endoscopic and histopathological features and treatments, who were hospitalized at department of pediatric gastroenterology of Shengjing Hospital of China Medical University between January 2012 and December 2017. Results The main clinical manifestations of EG were abdominal pain(75.8%), vomiting(30.3%)and diarrhea(21.2%). The hemoglobin level decreased in 7 cases(21.2%). The eosinophil count increased in 13 cases(39.4%). Total serum IgE elevated in 18 cases(54.5%), while 23 cases were positive in serum food or respiratory specific IgE.The fecal occult blood test showed positive in 7 cases(21.2%). Among 28 children conducted with whole abdominal CT, there were 13 cases of pelvis fluid, 12 cases of enlarged lymph nodes and 4 cases of thickened intestinal wall.All 33 children went through the endoscopic examination, the main histological change were erythema-exudation(31 cases, 93.9%), mucosal hyperemia edema(5 cases, 15.2%) and hemorrhage(4 cases, 12.1%). The main pathological change was mucosal inflammation with a large number of eosinophil infiltration(≥20 per HPF). Among the 33 cases, 17 cases relieved through treatment of avoiding allergen, antacids, anti-allergic agents and leukotriene receptor antagonist, with 1 case recurrence.The other 16 cases relieved after treatment of glucocorticoid and the above therapy, with 3 cases recurrence. Conclusion The clinical manifestations of children with EG are various and lack of specificity.In terms of diagnosis, the elevated level of eosinophil count and total serum IgE, or positive in serum food specific IgE may be taken as reference.The definite diagnosis is based on pathological examination (eosinophil infiltration≥20 per HPF). For treatment, avoiding allergen, antacids, anti-allergic agents and leukotriene receptor antagonist are effective in several children.Glucocorticoid can obtain satisfactory curative effect in the uneffective children. Key words: Eosinophilic gastroenteritis; Children; Clinical features; Endoscopy