BackgroundClinically, normalization of gait following anterior cruciate ligament reconstruction (ACLr) is defined as the absence of observable deviations. However, biomechanical studies report altered knee mechanics during loading response (LR); a time of double limb support and weight transfer between limbs. It is conceivable that subtle adjustments in whole body mechanics, including center of mass (COM) velocity and ground reaction force (GRF) peaks and timing, are present. Research questionThe purpose was to compare limb and whole body mechanics during LR of gait in the surgical and non-surgical limbs post-ACLr. MethodsAnterior and vertical COM velocity at initial contact; knee flexion range of motion, peak knee extensor moment, peak vertical and posterior GRF, minimum vertical COM position and maximum anterior and vertical COM velocity during LR were identified for twenty individuals 112 ± 17 days post-ACLr without observable gait deficits. To assess differences in timing of COM variables, coupling angles (vector coding) were calculated for multidirectional coordination of vertical and anteroposterior COM velocities and GRFs and categorized as in-phase, anti-phase, vertical phase, or anteroposterior phase coordination. Paired t-tests compared peaks between limbs; non-parametric Wilcoxon signed-rank tests compared coordination pattern frequency. ResultsLess knee range of motion (5.6 °), 30% smaller knee extensor moment, 11% smaller posterior GRF, and slower anterior COM velocity at initial contact (2%) and peak during LR (1.3%; all p < 0.05) were observed in the surgical compared to the non-surgical limb. For COM velocity coordination, lesser anti-phase (7.38%) and greater in-phase coordination (2.88%) were observed in the surgical limb. For GRF coordination, less in-phase coordination (1.94%) was observed in the surgical limb. SignificanceDifferences in coordination patterns, suggest that individuals post-ACLr make subtle adjustments in timing of whole body mechanics; particularly in COM velocity during gait. These adjustments are consistent with reduced sagittal plane loading in the surgical knee.