This study evaluated that grafting as an effective measure on resistance disease in continuous cropping system has a remarkable impact on improving the microbial community population and structure conditions in the rhizosphere of eggplant, which lead to a highly suppressive on the disease incidence. <i>Verticillium dahliae</i>, microbial community and soil enzyme activities in the rhizosphere of both grafted and self-rooted eggplants investigations were conducted in different eggplant growth stages in pot trials to determine the effects of grafting on the soil microbiological properties. In addition, an assessment of the bio-control effects of antagonistic microbes isolated from rhizosphere of grafted eggplants on the incidence of <i>Verticillium</i> wilt was performed. Grafted eggplants exhibited strong resistance (nearly 90%) with a lower pathological incident index. Amounts of <i>Verticillium dahliae</i> in the rhizosphere of grafted eggplants were lower before the fruiting stage compared to non-grafted self-rooting eggplants. Although each general classification of microbes (bacteria, actinomyces and fungi) was reduced over all growth stages by grafting, the ranges of bacterial and actinomycete reduction in the rhizosphere of grafted eggplants were lower compared to self-rooted eggplants. Experimental evidence indicated that grafting increased the relative ratios of Bacteria to Fungi and Actinomycete to Fungi. In terms of functional microbes, amounts of azotobacteria, ammonifying and nitrifying bacteria were significantly higher in grafted eggplants than self-rooted eggplants, especially after floral initiation. Compared to Control, grafting leaded to a significantly positive impacts on invertase, protein enzyme, urease, peroxidase and catalase activities in the rhizosphere of eggplants. Using antagonistic strains have showed strong suppression of <i>Verticillium dahliae</i> growth to study the prevention and curative effects on <i>Verticillium</i> wilt in pot trials.