ANTAGONISTIC AND DESTRUCTIVE ACTIVITY OF MARINE BACTERIA

Abstract

Aim. To investigate antagonistic, oil and surfactant-destructive activity of bacteria isolated from the coastal zones of the Black Sea. Methods. For isolation of bacteria-destructors from the sea and pore waters of the zone of splashes of the Odesa bay, the Zmiiny island there were used the method of accumulative crops on liquid synthetic medium MKD with further sowing on the nutrient medium MPB and M-9, contained separately oil sodium dodecyl sulfate in concentration of 1%. Researches of antagonistic activity, estimated according to the size of the growth inhibition zones of the test strains, were conducted by means of the method of the delayed crops. Results. The destructive and antagonistic activity of 58 strains of bacteria isolated from the coastal zones of the Black Sea was studied. The destructive activity in relation to oil and surfactants showed 36 strains studied. Of the most active, 3 strain-destructors were selected, which showed the greatest inhibitory effect on test strains of conditionally pathogenic microorganisms, including sanitary-indicative. The largest number of antagonistic strains is isolated from porous and sea water in the area of discharging urban residental sewage in the Dacha Kovalevsky, indicating the chronic chemical and biological contamination of the zone of splash in the area of the Odesa border of the Black Sea. Conclusions. Polyfunctional strains M. barkeri 5, M. barkeri 13, P. fluorescens 55, which showed a high level of antagonistic activity with respect to a wide spectrum of conditionally pathogenic microbiota representatives and had a high ability to oxidize hydrocarbon oil and surfactant, they can be recommended for further use in the biotechnology of remediation of marine waters from biological and chemical contamination.