Abstract Twenty-nine groundwater samples, collected from Wadi Nu'man wells, were analyzed for natural radioactivity to check for compliance with the national guideline values. Gross α and gross β screening, in addition to radon ( 222Rn) measurements, were performed for all samples. Samples of gross activity levels exceeding the national guideline values set out for drinking water were subjected to further investigation for radium and uranium isotopes using liquid scintillation counting and α-spectrometry, respectively. The results showed that the anomalous source was natural uranium. The water contains high concentrations of 222Rn and considerable levels of natural uranium. The 222Rn concentration ranged from 10–100 Bq/L with an average value of about 40 Bq/L. The uranium concentration, in samples of gross activity levels exceeding the national guideline value, ranged from 9 to 55 μg/L. The levels of 226Ra and 228Ra were below the detection limit of the counting system in all samples. The variation in the 222Rn and uranium concentrations was found to be linked with the local lithology. The higher values were recorded in the wells of Nu'man complex aquifer, where the predominating mineral is foliated monzonite. Recommendations and advices for water management to reduce radiation exposure to users are presented.