Electro analysis of 3-Aminopyridine (3-AP) by a simple and economic poly Eriochrome black T modified glassy carbon electrode (EBT/GC) was studied using cyclic voltammetric (CV) and square-wave voltammetric (SWV) techniques. The variation of the current with pH, concentration and scan rate was investigated to optimize the experimental condition for the determination of 3-AP. The electrochemical behavior of the 3-AP at poly EBT/GC was a diffusion-controlled process. A probable electro oxidation mechanism was proposed. Under the optimal conditions, the anodic peak current of SWV was linearly proportional to the concentration of 3-AP in the range from 2.0 × 10−6 to 1.2 × 10−5 M with a limit of detection 5.4 × 10−7 M. This method was applied for quantitative determination of 3-AP levels in urine and capsules as real samples. Further, study for interference was also made using some common interfering substances.